UniProtKB - Q96SB3 (NEB2_HUMAN)
Protein
Neurabin-2
Gene
PPP1R9B
Organism
Homo sapiens (Human)
Status
Functioni
Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth factor (HGF)-induced cell migration.By similarity1 Publication
GO - Molecular functioni
- actin filament binding Source: GO_Central
- D2 dopamine receptor binding Source: Ensembl
- ion channel binding Source: Ensembl
- kinase binding Source: Ensembl
- protein C-terminus binding Source: Ensembl
- protein kinase activity Source: Ensembl
- protein phosphatase 1 binding Source: UniProtKB
- protein phosphatase inhibitor activity Source: UniProtKB
GO - Biological processi
- actin filament depolymerization Source: Ensembl
- actin filament organization Source: GO_Central
- aging Source: Ensembl
- calcium-mediated signaling Source: GO_Central
- cell cycle arrest Source: UniProtKB
- cell migration Source: UniProtKB
- cellular response to drug Source: Ensembl
- cellular response to epidermal growth factor stimulus Source: Ensembl
- cellular response to estradiol stimulus Source: Ensembl
- cellular response to morphine Source: UniProtKB
- cellular response to peptide Source: Ensembl
- cerebral cortex development Source: Ensembl
- dendrite development Source: Ensembl
- developmental process involved in reproduction Source: Ensembl
- filopodium assembly Source: UniProtKB
- hippocampus development Source: Ensembl
- learning Source: Ensembl
- male mating behavior Source: Ensembl
- modulation of chemical synaptic transmission Source: InterPro
- negative regulation of cell growth Source: UniProtKB
- neuron projection development Source: GO_Central
- positive regulation of protein localization to actin cortical patch Source: Ensembl
- positive regulation of protein localization to plasma membrane Source: Ensembl
- protein localization to actin cytoskeleton Source: Ensembl
- protein localization to cell periphery Source: Ensembl
- regulation of cell growth by extracellular stimulus Source: UniProtKB
- regulation of cell population proliferation Source: UniProtKB
- regulation of exit from mitosis Source: UniProtKB
- regulation of opioid receptor signaling pathway Source: UniProtKB
- regulation of protein phosphorylation Source: Ensembl
- reproductive system development Source: Ensembl
- response to amphetamine Source: Ensembl
- response to clozapine Source: Ensembl
- response to immobilization stress Source: Ensembl
- response to kainic acid Source: Ensembl
- response to L-phenylalanine derivative Source: Ensembl
- response to nicotine Source: Ensembl
- response to prostaglandin E Source: Ensembl
- response to steroid hormone Source: Ensembl
- RNA splicing Source: UniProtKB
Keywordsi
Molecular function | Actin-binding, Developmental protein |
Biological process | Differentiation, Neurogenesis |
Enzyme and pathway databases
PathwayCommonsi | Q96SB3 |
SignaLinki | Q96SB3 |
SIGNORi | Q96SB3 |
Names & Taxonomyi
Protein namesi | Recommended name: Neurabin-2Alternative name(s): Neurabin-II Protein phosphatase 1 regulatory subunit 9B Spinophilin |
Gene namesi | Name:PPP1R9B Synonyms:PPP1R6 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9298, PPP1R9B |
MIMi | 603325, gene |
neXtProti | NX_Q96SB3 |
VEuPathDBi | HostDB:ENSG00000108819.10 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Plasma membrane
Cytoskeleton
- cytoskeleton By similarity
Cytoplasm and Cytosol
Other locations
- dendritic spine By similarity
- postsynaptic density By similarity
- synapse
- adherens junction By similarity
- lamellipodium
- filopodium
Note: Enriched at synapse and cadherin-based cell-cell adhesion sites. In neurons, both cytosolic and membrane-associated, and highly enriched in the postsynaptic density apposed to exitatory synapses. Colocalizes with PPP1R2 at actin-rich adherens junctions in epithelial cells and in dendritic spines (By similarity). Accumulates in the lamellipodium, filopodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.By similarity
Cytoskeleton
- actin cytoskeleton Source: GO_Central
- cortical actin cytoskeleton Source: Ensembl
Nucleus
- nucleoplasm Source: UniProtKB
Plasma Membrane
- cytoplasmic side of dendritic spine plasma membrane Source: Ensembl
- plasma membrane Source: UniProtKB
- ruffle membrane Source: UniProtKB
Other locations
- adherens junction Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB
- dendrite Source: GO_Central
- dendritic spine head Source: Ensembl
- dendritic spine neck Source: Ensembl
- filopodium Source: UniProtKB
- growth cone Source: Ensembl
- lamellipodium Source: UniProtKB
- neuronal cell body Source: Ensembl
- postsynaptic density Source: GO_Central
- protein phosphatase type 1 complex Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus, SynapsePathology & Biotechi
Organism-specific databases
DisGeNETi | 84687 |
OpenTargetsi | ENSG00000108819 |
PharmGKBi | PA33662 |
Miscellaneous databases
Pharosi | Q96SB3, Tdark |
Genetic variation databases
BioMutai | PPP1R9B |
DMDMi | 90101416 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000228614 | 1 – 817 | Neurabin-2Add BLAST | 817 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | PhosphoserineBy similarity | 1 | |
Modified residuei | 17 | PhosphoserineBy similarity | 1 | |
Modified residuei | 94 | PhosphoserineBy similarity | 1 | |
Modified residuei | 100 | PhosphoserineBy similarity | 1 | |
Modified residuei | 116 | PhosphoserineBy similarity | 1 | |
Modified residuei | 192 | PhosphoserineCombined sources | 1 | |
Modified residuei | 193 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 205 | PhosphoserineCombined sources | 1 | |
Modified residuei | 207 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 438 | PhosphoserineCombined sources | 1 | |
Modified residuei | 658 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1 (By similarity).By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q96SB3 |
jPOSTi | Q96SB3 |
MassIVEi | Q96SB3 |
MaxQBi | Q96SB3 |
PeptideAtlasi | Q96SB3 |
PRIDEi | Q96SB3 |
ProteomicsDBi | 78097 |
PTM databases
iPTMneti | Q96SB3 |
PhosphoSitePlusi | Q96SB3 |
Expressioni
Gene expression databases
Bgeei | ENSG00000108819, Expressed in cerebellar vermis and 201 other tissues |
Interactioni
Subunit structurei
Interacts with DCLK2 (By similarity). Possibly exists as a homodimer, homotrimer or a homotetramer.
Interacts with F-actin, PPP1CA, neurabin-1, TGN38 and D2 dopamine receptor.
Interacts with RGS1, RGS2, RGS4, RGS19 and ADRA1B, ADRA2A, ADRA2B, ADRA2C, CDKN2A, PPP1R2, RASGFR1 and TIAM1.
Interacts (via C-terminus) with SPATA13 (via C-terminal tail).
Interacts with ADRA2B.
By similarity3 PublicationsBinary interactionsi
Hide detailsQ96SB3
With | #Exp. | IntAct |
---|---|---|
PPP1CA [P62136] | 6 | EBI-351275,EBI-357253 |
PPP1CB [P62140] | 2 | EBI-351275,EBI-352350 |
PPP1CC [P36873] | 5 | EBI-351275,EBI-356283 |
GO - Molecular functioni
- actin filament binding Source: GO_Central
- D2 dopamine receptor binding Source: Ensembl
- ion channel binding Source: Ensembl
- kinase binding Source: Ensembl
- protein C-terminus binding Source: Ensembl
- protein phosphatase 1 binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 124202, 81 interactors |
IntActi | Q96SB3, 43 interactors |
MINTi | Q96SB3 |
STRINGi | 9606.ENSP00000478767 |
Miscellaneous databases
RNActi | Q96SB3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 496 – 584 | PDZPROSITE-ProRule annotationAdd BLAST | 89 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 154 | Actin-bindingBy similarityAdd BLAST | 154 | |
Regioni | 100 – 371 | Interaction with D(2) dopamine receptorBy similarityAdd BLAST | 272 | |
Regioni | 164 – 283 | Actin-bindingBy similarityAdd BLAST | 120 | |
Regioni | 169 – 255 | Interaction with ADRA2A, ADRA2B and ADRA2CBy similarityAdd BLAST | 87 | |
Regioni | 417 – 494 | Interaction with protein phosphatase 1By similarityAdd BLAST | 78 | |
Regioni | 480 – 525 | Interaction with RGS2By similarityAdd BLAST | 46 | |
Regioni | 595 – 816 | Interaction with TGN38By similarityAdd BLAST | 222 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 671 – 788 | Sequence analysisAdd BLAST | 118 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 447 – 451 | PP1-binding motifBy similarity | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 253 – 261 | Poly-Pro | 9 |
Domaini
The PP1 binding region is natively unstructured, upon PP1 binding, it acquires structure, blocks a substrate-binding site, and restricts PP1 phosphatase specificity to a subset of substrates.By similarity
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1945, Eukaryota |
GeneTreei | ENSGT00940000158833 |
HOGENOMi | CLU_007401_1_0_1 |
InParanoidi | Q96SB3 |
OMAi | PESSCME |
OrthoDBi | 128743at2759 |
PhylomeDBi | Q96SB3 |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR029921, NEB2 IPR040645, Neurabin-1/2_PDZ IPR043446, Neurabin-like IPR001478, PDZ IPR036034, PDZ_sf |
PANTHERi | PTHR16154, PTHR16154, 1 hit PTHR16154:SF24, PTHR16154:SF24, 1 hit |
Pfami | View protein in Pfam PF00595, PDZ, 1 hit PF17817, PDZ_5, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS50106, PDZ, 1 hit |
i Sequence
Sequence statusi: Complete.
Q96SB3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMKTEPRGPG GPLRSASPHR SAYEAGIQAL KPPDAPGPDE APKGAHHKKY
60 70 80 90 100
GSNVHRIKSM FLQMGTTAGP SGEAGGGAGL AEAPRASERG VRLSLPRASS
110 120 130 140 150
LNENVDHSAL LKLGTSVSER VSRFDSKPAP SAQPAPPPHP PSRLQETRKL
160 170 180 190 200
FERSAPAAAG GDKEAAARRL LRQERAGLQD RKLDVVVRFN GSTEALDKLD
210 220 230 240 250
ADAVSPTVSQ LSAVFEKADS RTGLHRGPGL PRAAGVPQVN SKLVSKRSRV
260 270 280 290 300
FQPPPPPPPA PSGDAPAEKE RCPAGQQPPQ HRVAPARPPP KPREVRKIKP
310 320 330 340 350
VEVEESGESE AESAPGEVIQ AEVTVHAALE NGSTVATAAS PAPEEPKAQA
360 370 380 390 400
APEKEAAAVA PPERGVGNGR APDVAPEEVD ESKKEDFSEA DLVDVSAYSG
410 420 430 440 450
LGEDSAGSAL EEDDEDDEED GEPPYEPESG CVEIPGLSEE EDPAPSRKIH
460 470 480 490 500
FSTAPIQVFS TYSNEDYDRR NEDVDPMAAS AEYELEKRVE RLELFPVELE
510 520 530 540 550
KDSEGLGISI IGMGAGADMG LEKLGIFVKT VTEGGAAHRD GRIQVNDLLV
560 570 580 590 600
EVDGTSLVGV TQSFAASVLR NTKGRVRFMI GRERPGEQSE VAQLIQQTLE
610 620 630 640 650
QERWQREMME QRYAQYGEDD EETGEYATDE DEELSPTFPG GEMAIEVFEL
660 670 680 690 700
AENEDALSPV DMEPEKLVHK FKELQIKHAV TEAEIQQLKR KLQSLEQEKG
710 720 730 740 750
RWRVEKAQLE QSVEENKERM EKLEGYWGEA QSLCQAVDEH LRETQAQYQA
760 770 780 790 800
LERKYSKAKR LIKDYQQKEI EFLKKETAQR RVLEESELAR KEEMDKLLDK
810
ISELEGNLQT LRNSNST
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 159 | A → G in CAC37685 (PubMed:11278317).Curated | 1 | |
Sequence conflicti | 161 | Missing in CAC37685 (PubMed:11278317).Curated | 1 | |
Sequence conflicti | 165 | Missing in CAC37685 (PubMed:11278317).Curated | 1 | |
Sequence conflicti | 576 | V → VRC in CAC37685 (PubMed:11278317).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_059776 | 201 | A → T. Corresponds to variant dbSNP:rs8079707Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ401189 mRNA Translation: CAC37685.1 ABBA01041227 Genomic DNA No translation available. AC002401 Genomic DNA No translation available. KF495721 Genomic DNA No translation available. CH471109 Genomic DNA Translation: EAW94638.1 CH471109 Genomic DNA Translation: EAW94637.1 AL713642 mRNA Translation: CAD28455.2 |
CCDSi | CCDS74102.1 |
RefSeqi | NP_115984.3, NM_032595.4 |
Genome annotation databases
Ensembli | ENST00000612501; ENSP00000478767; ENSG00000108819 |
GeneIDi | 84687 |
KEGGi | hsa:84687 |
UCSCi | uc032fmj.2, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ401189 mRNA Translation: CAC37685.1 ABBA01041227 Genomic DNA No translation available. AC002401 Genomic DNA No translation available. KF495721 Genomic DNA No translation available. CH471109 Genomic DNA Translation: EAW94638.1 CH471109 Genomic DNA Translation: EAW94637.1 AL713642 mRNA Translation: CAD28455.2 |
CCDSi | CCDS74102.1 |
RefSeqi | NP_115984.3, NM_032595.4 |
3D structure databases
BMRBi | Q96SB3 |
SMRi | Q96SB3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 124202, 81 interactors |
IntActi | Q96SB3, 43 interactors |
MINTi | Q96SB3 |
STRINGi | 9606.ENSP00000478767 |
PTM databases
iPTMneti | Q96SB3 |
PhosphoSitePlusi | Q96SB3 |
Genetic variation databases
BioMutai | PPP1R9B |
DMDMi | 90101416 |
Proteomic databases
EPDi | Q96SB3 |
jPOSTi | Q96SB3 |
MassIVEi | Q96SB3 |
MaxQBi | Q96SB3 |
PeptideAtlasi | Q96SB3 |
PRIDEi | Q96SB3 |
ProteomicsDBi | 78097 |
Protocols and materials databases
Antibodypediai | 30484, 146 antibodies |
Genome annotation databases
Ensembli | ENST00000612501; ENSP00000478767; ENSG00000108819 |
GeneIDi | 84687 |
KEGGi | hsa:84687 |
UCSCi | uc032fmj.2, human |
Organism-specific databases
CTDi | 84687 |
DisGeNETi | 84687 |
GeneCardsi | PPP1R9B |
HGNCi | HGNC:9298, PPP1R9B |
MIMi | 603325, gene |
neXtProti | NX_Q96SB3 |
OpenTargetsi | ENSG00000108819 |
PharmGKBi | PA33662 |
VEuPathDBi | HostDB:ENSG00000108819.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1945, Eukaryota |
GeneTreei | ENSGT00940000158833 |
HOGENOMi | CLU_007401_1_0_1 |
InParanoidi | Q96SB3 |
OMAi | PESSCME |
OrthoDBi | 128743at2759 |
PhylomeDBi | Q96SB3 |
Enzyme and pathway databases
PathwayCommonsi | Q96SB3 |
SignaLinki | Q96SB3 |
SIGNORi | Q96SB3 |
Miscellaneous databases
BioGRID-ORCSi | 84687, 14 hits in 171 CRISPR screens |
ChiTaRSi | PPP1R9B, human |
GeneWikii | PPP1R9B |
GenomeRNAii | 84687 |
Pharosi | Q96SB3, Tdark |
PROi | PR:Q96SB3 |
RNActi | Q96SB3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000108819, Expressed in cerebellar vermis and 201 other tissues |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR029921, NEB2 IPR040645, Neurabin-1/2_PDZ IPR043446, Neurabin-like IPR001478, PDZ IPR036034, PDZ_sf |
PANTHERi | PTHR16154, PTHR16154, 1 hit PTHR16154:SF24, PTHR16154:SF24, 1 hit |
Pfami | View protein in Pfam PF00595, PDZ, 1 hit PF17817, PDZ_5, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS50106, PDZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NEB2_HUMAN | |
Accessioni | Q96SB3Primary (citable) accession number: Q96SB3 Secondary accession number(s): D3DTX6, Q8TCR9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2006 |
Last sequence update: | May 8, 2019 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot