PPP1R9B

Functionⁱ

Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth factor (HGF)-induced cell migration.By similarity1 Publication

GO - Molecular functionⁱ

actin filament binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein phosphatase 1 binding
Source: UniProtKB
Non-traceable author statementⁱ
- 11278317
protein phosphatase inhibitor activity
Source: UniProtKB
Non-traceable author statementⁱ
- 11278317

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin filament organization
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
calcium-mediated signaling
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
cell cycle arrest
Source: UniProtKB
Traceable author statementⁱ
- 11278317
cell migration
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19151759
cellular response to morphine Source: UniProtKB
filopodium assembly
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19151759
modulation of chemical synaptic transmission Source: InterPro
negative regulation of cell growth
Source: UniProtKB
Inferred from direct assayⁱ
- 11278317
neuron projection development
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of cell growth by extracellular stimulus
Source: UniProtKB
Traceable author statementⁱ
- 11278317
regulation of cell proliferation
Source: UniProtKB
Non-traceable author statementⁱ
- 11278317
regulation of exit from mitosis
Source: UniProtKB
Non-traceable author statementⁱ
- 11278317
regulation of opioid receptor signaling pathway Source: UniProtKB
RNA splicing
Source: UniProtKB
Non-traceable author statementⁱ
- 11278317

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Actin-binding, Developmental protein
Biological process	Differentiation, Neurogenesis

Enzyme and pathway databases

SignaLinkⁱ	Q96SB3
SIGNORⁱ	Q96SB3

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Neurabin-2 Alternative name(s): Neurabin-II Protein phosphatase 1 regulatory subunit 9B Spinophilin
Gene namesⁱ	Name:PPP1R9B Synonyms:PPP1R6
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Unplaced

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:9298 PPP1R9B
MIMⁱ	603325 gene
neXtProtⁱ	NX_Q96SB3

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus, Synapse

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	84687
PharmGKBⁱ	PA33662

Polymorphism and mutation databases

BioMutaⁱ	PPP1R9B
DMDMⁱ	90101416

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000228614	1 – 815	Neurabin-2Add BLAST		815

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	15	PhosphoserineBy similarity	1
Modified residueⁱ	17	PhosphoserineBy similarity	1
Modified residueⁱ	94	PhosphoserineBy similarity	1
Modified residueⁱ	100	PhosphoserineBy similarity	1
Modified residueⁱ	116	PhosphoserineBy similarity	1
Modified residueⁱ	190	PhosphoserineCombined sources	1
Modified residueⁱ	191	PhosphothreonineCombined sources	1
Modified residueⁱ	203	PhosphoserineCombined sources	1
Modified residueⁱ	205	PhosphothreonineCombined sources	1
Modified residueⁱ	436	PhosphoserineCombined sources	1
Modified residueⁱ	656	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1 (By similarity).By similarity

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q96SB3
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q96SB3
PeptideAtlas More...PeptideAtlasⁱ	Q96SB3
PRIDEⁱ	Q96SB3
ProteomicsDBⁱ	78097

PTM databases

iPTMnetⁱ	Q96SB3
PhosphoSitePlusⁱ	Q96SB3

Expressionⁱ

Gene expression databases

CleanExⁱ	HS_PPP1R9B

CleanExⁱ

HS_PPP1R9B

Interactionⁱ

Subunit structureⁱ

Interacts with DCLK2 (By similarity). Possibly exists as a homodimer, homotrimer or a homotetramer. Interacts with F-actin, PPP1CA, neurabin-1, TGN38 and D₂ dopamine receptor. Interacts with RGS1, RGS2, RGS4, RGS19 and ADRA1B, ADRA2A, ADRA2B, ADRA2C, CDKN2A, PPP1R2, RASGFR1 and TIAM1. Interacts (via C-terminus) with SPATA13 (via C-terminal tail). Interacts with ADRA2B.By similarity3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
PPP1CA	P62136	5	EBI-351275,EBI-357253
PPP1CB	P62140	2	EBI-351275,EBI-352350
PPP1CC	P36873	5	EBI-351275,EBI-356283

GO - Molecular functionⁱ

actin filament binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein phosphatase 1 binding
Source: UniProtKB
Non-traceable author statementⁱ
- 11278317

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	124202, 49 interactors
IntActⁱ	Q96SB3, 36 interactors
Molecular INTeraction database More...MINTⁱ	Q96SB3

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q96SB3
SMRⁱ	Q96SB3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	494 – 582	PDZPROSITE-ProRule annotationAdd BLAST		89

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 154	Actin-bindingBy similarityAdd BLAST	154
Regionⁱ	100 – 369	Interaction with D(2) dopamine receptorBy similarityAdd BLAST	270
Regionⁱ	163 – 281	Actin-bindingBy similarityAdd BLAST	119
Regionⁱ	167 – 253	Interaction with ADRA2A, ADRA2B and ADRA2CBy similarityAdd BLAST	87
Regionⁱ	415 – 492	Interaction with protein phosphatase 1By similarityAdd BLAST	78
Regionⁱ	478 – 523	Interaction with RGS2By similarityAdd BLAST	46
Regionⁱ	593 – 814	Interaction with TGN38By similarityAdd BLAST	222

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	593 – 614	Sequence analysisAdd BLAST		22
Coiled coilⁱ	663 – 814	Sequence analysisAdd BLAST		152

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	445 – 449	PP1-binding motifBy similarity		5

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	251 – 259	Poly-Pro		9

Domainⁱ

The PP1 binding region is natively unstructured, upon PP1 binding, it acquires structure, blocks a substrate-binding site, and restricts PP1 phosphatase specificity to a subset of substrates.By similarity

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

HOVERGENⁱ	HBG005213
InParanoidⁱ	Q96SB3
KEGG Orthology (KO) More...KOⁱ	K17551
PhylomeDBⁱ	Q96SB3

Family and domain databases

InterProⁱ	View protein in InterPro IPR029921 NEB2 IPR001478 PDZ IPR036034 PDZ_sf
PANTHERⁱ	PTHR16154:SF24 PTHR16154:SF24, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00595 PDZ, 1 hit
SMARTⁱ	View protein in SMART SM00228 PDZ, 1 hit
SUPFAMⁱ	SSF50156 SSF50156, 1 hit
PROSITEⁱ	View protein in PROSITE PS50106 PDZ, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q96SB3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MMKTEPRGPG GPLRSASPHR SAYEAGIQAL KPPDAPGPDE APKGAHHKKY 
        60         70         80         90        100
GSNVHRIKSM FLQMGTTAGP SGEAGGGAGL AEAPRASERG VRLSLPRASS 
       110        120        130        140        150
LNENVDHSAL LKLGTSVSER VSRFDSKPAP SAQPAPPPHP PSRLQETRKL 
       160        170        180        190        200
FERSAPAAGG DKEAARRLLR QERAGLQDRK LDVVVRFNGS TEALDKLDAD 
       210        220        230        240        250
AVSPTVSQLS AVFEKADSRT GLHRGPGLPR AAGVPQVNSK LVSKRSRVFQ 
       260        270        280        290        300
PPPPPPPAPS GDAPAEKERC PAGQQPPQHR VAPARPPPKP REVRKIKPVE 
       310        320        330        340        350
VEESGESEAE SAPGEVIQAE VTVHAALENG STVATAASPA PEEPKAQAAP 
       360        370        380        390        400
EKEAAAVAPP ERGVGNGRAP DVAPEEVDES KKEDFSEADL VDVSAYSGLG 
       410        420        430        440        450
EDSAGSALEE DDEDDEEDGE PPYEPESGCV EIPGLSEEED PAPSRKIHFS 
       460        470        480        490        500
TAPIQVFSTY SNEDYDRRNE DVDPMAASAE YELEKRVERL ELFPVELEKD 
       510        520        530        540        550
SEGLGISIIG MGAGADMGLE KLGIFVKTVT EGGAAHRDGR IQVNDLLVEV 
       560        570        580        590        600
DGTSLVGVTQ SFAASVLRNT KGRVRFMIGR ERPGEQSEVA QLIQQTLEQE 
       610        620        630        640        650
RWQREMMEQR YAQYGEDDEE TGEYATDEDE ELSPTFPGGE MAIEVFELAE 
       660        670        680        690        700
NEDALSPVDM EPEKLVHKFK ELQIKHAVTE AEIQQLKRKL QSLEQEKGRW 
       710        720        730        740        750
RVEKAQLEQS VEENKERMEK LEGYWGEAQS LCQAVDEHLR ETQAQYQALE 
       760        770        780        790        800
RKYSKAKRLI KDYQQKEIEF LKKETAQRRV LEESELARKE EMDKLLDKIS 
       810 
ELEGNLQTLR NSNST

Length:815

Mass (Da):89,192

Last modified:March 21, 2006 - v2

Checksum:ⁱD004CAA7030D3267

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	D3DTX6	D3DTX6_HUMAN		Neurabin-2 Neurabin-2 (Protein phosphatase 1, regulatory subunit 9B, spinophilin, isoform CRA_b)	PPP1R9B hCG_29611	817	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	574	V → VRC in CAC37685 (PubMed:11278317).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_059776	199	A → T. Corresponds to variant dbSNP:rs8079707Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ401189 mRNA Translation: CAC37685.1 AL713642 mRNA Translation: CAD28455.2
NCBI Reference Sequences More...RefSeqⁱ	NP_115984.3, NM_032595.4
UniGeneⁱ	Hs.514323

Genome annotation databases

Ensemblⁱ	ENST00000316878; ENSP00000475417; ENSG00000108819
GeneIDⁱ	84687
KEGGⁱ	hsa:84687

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q96SB3	neurabin-2		ERIEU		817	UniRef90_Q96SB3
Uncharacterized protein		CHLSB		816
Uncharacterized protein		NEOLE		816
Neurabin-2		CALJA		815
Uncharacterized protein		Cebus capucinus imitator		815
+21

Protein	Similar proteins		Species	Score	Length	Source
Q96SB3	Neurabin-2	)	RAT		817	UniRef50_O35274
Neurabin-2		MOUSE		817
Protein phosphatase 1 regulatory subunit 9B		CANLF		830
Uncharacterized protein		FELCA		821
Uncharacterized protein		CAVPO		821
+129

Cross-referencesⁱ

Web resourcesⁱ

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ401189 mRNA Translation: CAC37685.1 AL713642 mRNA Translation: CAD28455.2
RefSeqⁱ	NP_115984.3, NM_032595.4
UniGeneⁱ	Hs.514323

3D structure databases

ProteinModelPortalⁱ	Q96SB3
SMRⁱ	Q96SB3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	124202, 49 interactors
IntActⁱ	Q96SB3, 36 interactors
MINTⁱ	Q96SB3

PTM databases

iPTMnetⁱ	Q96SB3
PhosphoSitePlusⁱ	Q96SB3

Polymorphism and mutation databases

BioMutaⁱ	PPP1R9B
DMDMⁱ	90101416

Proteomic databases

EPDⁱ	Q96SB3
MaxQBⁱ	Q96SB3
PeptideAtlasⁱ	Q96SB3
PRIDEⁱ	Q96SB3
ProteomicsDBⁱ	78097

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000316878; ENSP00000475417; ENSG00000108819
GeneIDⁱ	84687
KEGGⁱ	hsa:84687

Organism-specific databases

CTDⁱ	84687
DisGeNETⁱ	84687
GeneCardsⁱ	PPP1R9B
H-InvDBⁱ	HIX0202435
HGNCⁱ	HGNC:9298 PPP1R9B
MIMⁱ	603325 gene
neXtProtⁱ	NX_Q96SB3
PharmGKBⁱ	PA33662
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

HOVERGENⁱ	HBG005213
InParanoidⁱ	Q96SB3
KOⁱ	K17551
PhylomeDBⁱ	Q96SB3

Enzyme and pathway databases

SignaLinkⁱ	Q96SB3
SIGNORⁱ	Q96SB3

Miscellaneous databases

ChiTaRSⁱ	PPP1R9B human
GeneWikiⁱ	PPP1R9B
GenomeRNAiⁱ	84687
Protein Ontology More...PROⁱ	PR:Q96SB3
SOURCEⁱ	Search...

Gene expression databases

CleanExⁱ	HS_PPP1R9B

CleanExⁱ

HS_PPP1R9B

Family and domain databases

InterProⁱ	View protein in InterPro IPR029921 NEB2 IPR001478 PDZ IPR036034 PDZ_sf
PANTHERⁱ	PTHR16154:SF24 PTHR16154:SF24, 1 hit
Pfamⁱ	View protein in Pfam PF00595 PDZ, 1 hit
SMARTⁱ	View protein in SMART SM00228 PDZ, 1 hit
SUPFAMⁱ	SSF50156 SSF50156, 1 hit
PROSITEⁱ	View protein in PROSITE PS50106 PDZ, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NEB2_HUMAN
Accessionⁱ	Q96SB3Primary (citable) accession number: Q96SB3 Secondary accession number(s): Q8TCR9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
	Last sequence update:	March 21, 2006
	Last modified:	November 7, 2018
	This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM

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UniProtKB - Q96SB3 (NEB2_HUMAN)

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Neurabin-2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Nucleus

Cytoskeleton

Other locations

Cytoskeleton

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Coiled coil

Motif

Compositional bias

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ