UniProtKB - Q96RN5 (MED15_HUMAN)
Mediator of RNA polymerase II transcription subunit 15
MED15
Functioni
GO - Molecular functioni
- transcription coregulator activity Source: InterPro
GO - Biological processi
- regulation of transcription by RNA polymerase II Source: InterPro
- stem cell population maintenance Source: Ensembl
- transcription initiation from RNA polymerase II promoter Source: Reactome
Keywordsi
Molecular function | Activator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q96RN5 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-212436, Generic Transcription Pathway R-HSA-381340, Transcriptional regulation of white adipocyte differentiation |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 15Alternative name(s): Activator-recruited cofactor 105 kDa component Short name: ARC105 CTG repeat protein 7a Mediator complex subunit 15 Positive cofactor 2 glutamine/Q-rich-associated protein Short name: PC2 glutamine/Q-rich-associated protein TPA-inducible gene 1 protein Short name: TIG-1 Trinucleotide repeat-containing gene 7 protein |
Gene namesi | Name:MED15 Synonyms:ARC105, CTG7A, PCQAP, TIG1, TNRC7 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000099917.17 |
HGNCi | HGNC:14248, MED15 |
MIMi | 607372, gene |
neXtProti | NX_Q96RN5 |
Subcellular locationi
Nucleus
- mediator complex Source: InterPro
- nucleoplasm Source: HPA
Other locations
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 42 | E → A: Abrogates interaction with SREBF1. 1 Publication | 1 | |
Mutagenesisi | 58 | L → D: Abrogates interaction with SREBF1. 1 Publication | 1 | |
Mutagenesisi | 60 | A → D: Abrogates interaction with SREBF1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 51586 |
OpenTargetsi | ENSG00000099917 |
PharmGKBi | PA33088 |
Miscellaneous databases
Pharosi | Q96RN5, Tbio |
Polymorphism and mutation databases
BioMutai | MED15 |
DMDMi | 27734440 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058264 | 1 – 788 | Mediator of RNA polymerase II transcription subunit 15Add BLAST | 788 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 349 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 603 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q96RN5 |
jPOSTi | Q96RN5 |
MassIVEi | Q96RN5 |
MaxQBi | Q96RN5 |
PaxDbi | Q96RN5 |
PeptideAtlasi | Q96RN5 |
PRIDEi | Q96RN5 |
ProteomicsDBi | 77993 [Q96RN5-1] 77994 [Q96RN5-2] 77995 [Q96RN5-3] |
PTM databases
iPTMneti | Q96RN5 |
PhosphoSitePlusi | Q96RN5 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000099917, Expressed in sural nerve and 224 other tissues |
ExpressionAtlasi | Q96RN5, baseline and differential |
Genevisiblei | Q96RN5, HS |
Organism-specific databases
HPAi | ENSG00000099917, Low tissue specificity |
Interactioni
Subunit structurei
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
Interacts with SMAD2, SMAD3, SREBF1 and SREBF2.
Interacts with WWTR1.
Interacts with TRIM11.
7 PublicationsBinary interactionsi
Hide detailsQ96RN5
Protein-protein interaction databases
BioGRIDi | 119623, 94 interactors |
ComplexPortali | CPX-3227, Core mediator complex |
CORUMi | Q96RN5 |
DIPi | DIP-32908N |
IntActi | Q96RN5, 43 interactors |
MINTi | Q96RN5 |
STRINGi | 9606.ENSP00000263205 |
Miscellaneous databases
RNActi | Q96RN5, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q96RN5 |
SMRi | Q96RN5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96RN5 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 9 – 73 | Interaction with SREBF11 PublicationAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 547 – 564 | Nuclear localization signalSequence analysisAdd BLAST | 18 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 161 – 174 | Poly-GlnAdd BLAST | 14 | |
Compositional biasi | 178 – 193 | Poly-GlnAdd BLAST | 16 | |
Compositional biasi | 205 – 218 | Poly-GlnAdd BLAST | 14 | |
Compositional biasi | 226 – 239 | Poly-GlnAdd BLAST | 14 | |
Compositional biasi | 243 – 262 | Poly-GlnAdd BLAST | 20 | |
Compositional biasi | 266 – 515 | Pro-richAdd BLAST | 250 | |
Compositional biasi | 360 – 367 | Poly-Gln | 8 | |
Compositional biasi | 449 – 456 | Poly-Pro | 8 | |
Compositional biasi | 602 – 611 | Poly-Pro | 10 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4274, Eukaryota |
GeneTreei | ENSGT00730000111140 |
HOGENOMi | CLU_023921_0_0_1 |
InParanoidi | Q96RN5 |
OMAi | QQNQMGM |
PhylomeDBi | Q96RN5 |
TreeFami | TF324988 |
Family and domain databases
Gene3Di | 1.10.246.20, 1 hit |
InterProi | View protein in InterPro IPR036529, KIX_dom_sf IPR019087, Med15 |
Pfami | View protein in Pfam PF09606, Med15, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 15 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDVSGQETDW RSTAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK
60 70 80 90 100
TRDEYLSLVA RLIIHFRDIH NKKSQASVSD PMNALQSLTG GPAAGAAGIG
110 120 130 140 150
MPPRGPGQSL GGMGSLGAMG QPMSLSGQPP PGTSGMAPHS MAVVSTATPQ
160 170 180 190 200
TQLQLQQVAL QQQQQQQQFQ QQQQAALQQQ QQQQQQQQFQ AQQSAMQQQF
210 220 230 240 250
QAVVQQQQQL QQQQQQQQHL IKLHHQNQQQ IQQQQQQLQR IAQLQLQQQQ
260 270 280 290 300
QQQQQQQQQQ QQALQAQPPI QQPPMQQPQP PPSQALPQQL QQMHHTQHHQ
310 320 330 340 350
PPPQPQQPPV AQNQPSQLPP QSQTQPLVSQ AQALPGQMLY TQPPLKFVRA
360 370 380 390 400
PMVVQQPPVQ PQVQQQQTAV QTAQAAQMVA PGVQMITEAL AQGGMHIRAR
410 420 430 440 450
FPPTTAVSAI PSSSIPLGRQ PMAQVSQSSL PMLSSPSPGQ QVQTPQSMPP
460 470 480 490 500
PPQPSPQPGQ PSSQPNSNVS SGPAPSPSSF LPSPSPQPSQ SPVTARTPQN
510 520 530 540 550
FSVPSPGPLN TPVNPSSVMS PAGSSQAEEQ QYLDKLKQLS KYIEPLRRMI
560 570 580 590 600
NKIDKNEDRK KDLSKMKSLL DILTDPSKRC PLKTLQKCEI ALEKLKNDMA
610 620 630 640 650
VPTPPPPPVP PTKQQYLCQP LLDAVLANIR SPVFNHSLYR TFVPAMTAIH
660 670 680 690 700
GPPITAPVVC TRKRRLEDDE RQSIPSVLQG EVARLDPKFL VNLDPSHCSN
710 720 730 740 750
NGTVHLICKL DDKDLPSVPP LELSVPADYP AQSPLWIDRQ WQYDANPFLQ
760 770 780
SVHRCMTSRL LQLPDKHSVT ALLNTWAQSV HQACLSAA
Computationally mapped potential isoform sequencesi
There are 15 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V1P5 | G3V1P5_HUMAN | Mediator of RNA polymerase II trans... | MED15 PCQAP, hCG_41277 | 722 | Annotation score: | ||
C9J1I1 | C9J1I1_HUMAN | Mediator of RNA polymerase II trans... | MED15 | 141 | Annotation score: | ||
C9JGN2 | C9JGN2_HUMAN | Mediator of RNA polymerase II trans... | MED15 | 124 | Annotation score: | ||
C9JZV5 | C9JZV5_HUMAN | Mediator of RNA polymerase II trans... | MED15 | 120 | Annotation score: | ||
C9JM58 | C9JM58_HUMAN | Mediator of RNA polymerase II trans... | MED15 | 105 | Annotation score: | ||
C9JJ12 | C9JJ12_HUMAN | Mediator of RNA polymerase II trans... | MED15 | 160 | Annotation score: | ||
F2Z2B7 | F2Z2B7_HUMAN | Mediator of RNA polymerase II trans... | MED15 | 55 | Annotation score: | ||
C9JCQ3 | C9JCQ3_HUMAN | Mediator of RNA polymerase II trans... | MED15 | 165 | Annotation score: | ||
C9JLN7 | C9JLN7_HUMAN | Mediator of RNA polymerase II trans... | MED15 | 105 | Annotation score: | ||
C9JWK5 | C9JWK5_HUMAN | Mediator of RNA polymerase II trans... | MED15 | 82 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 12 | S → R in AAC12944 (PubMed:11024300).Curated | 1 | |
Sequence conflicti | 116 | L → F in AAC12944 (PubMed:11024300).Curated | 1 | |
Sequence conflicti | 154 | Q → H in BAC03446 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 161 | Q → R in BAB85034 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 185 – 186 | QQ → EL in AAB91443 (PubMed:9225980).Curated | 2 | |
Sequence conflicti | 186 – 187 | Missing in CAG30423 (PubMed:15461802).Curated | 2 | |
Sequence conflicti | 218 | Missing in CAG30423 (PubMed:15461802).Curated | 1 | |
Sequence conflicti | 232 – 287 | Missing in BAB85034 (PubMed:14702039).CuratedAdd BLAST | 56 | |
Sequence conflicti | 265 | Q → E in AAC12944 (PubMed:11024300).Curated | 1 | |
Sequence conflicti | 265 | Q → E in AAB91443 (PubMed:9225980).Curated | 1 | |
Sequence conflicti | 572 – 573 | IL → GI in AAB91443 (PubMed:9225980).Curated | 2 | |
Sequence conflicti | 685 | L → V in BAB85034 (PubMed:14702039).Curated | 1 |
Polymorphismi
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_013136 | 261 – 262 | Missing . | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013024 | 80 – 151 | DPMNA…ATPQT → A in isoform 3. 1 PublicationAdd BLAST | 72 | |
Alternative sequenceiVSP_003922 | 385 – 424 | Missing in isoform 2 and isoform 3. 6 PublicationsAdd BLAST | 40 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF056191 mRNA Translation: AAC12944.1 Frameshift. AF328769 mRNA Translation: AAK58423.1 AK074268 mRNA Translation: BAB85034.1 Sequence problems. AK090465 mRNA Translation: BAC03446.1 Different initiation. CR456537 mRNA Translation: CAG30423.1 CH471176 Genomic DNA Translation: EAX02951.1 CH471176 Genomic DNA Translation: EAX02952.1 CH471176 Genomic DNA Translation: EAX02954.1 CH471176 Genomic DNA Translation: EAX02956.1 BC007529 mRNA Translation: AAH07529.1 BC013985 mRNA Translation: AAH13985.1 U80745 mRNA Translation: AAB91443.1 |
CCDSi | CCDS13781.1 [Q96RN5-2] CCDS33602.1 [Q96RN5-1] CCDS74824.1 [Q96RN5-3] |
RefSeqi | NP_001003891.1, NM_001003891.2 [Q96RN5-1] NP_001280164.1, NM_001293235.1 NP_001280165.1, NM_001293236.1 [Q96RN5-3] NP_056973.2, NM_015889.4 [Q96RN5-2] |
Genome annotation databases
Ensembli | ENST00000263205; ENSP00000263205; ENSG00000099917 [Q96RN5-1] ENST00000292733; ENSP00000292733; ENSG00000099917 [Q96RN5-2] ENST00000382974; ENSP00000372434; ENSG00000099917 [Q96RN5-3] |
GeneIDi | 51586 |
KEGGi | hsa:51586 |
UCSCi | uc002zsp.4, human [Q96RN5-1] |
Keywords - Coding sequence diversityi
Alternative splicing, Polymorphism, Triplet repeat expansionSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF056191 mRNA Translation: AAC12944.1 Frameshift. AF328769 mRNA Translation: AAK58423.1 AK074268 mRNA Translation: BAB85034.1 Sequence problems. AK090465 mRNA Translation: BAC03446.1 Different initiation. CR456537 mRNA Translation: CAG30423.1 CH471176 Genomic DNA Translation: EAX02951.1 CH471176 Genomic DNA Translation: EAX02952.1 CH471176 Genomic DNA Translation: EAX02954.1 CH471176 Genomic DNA Translation: EAX02956.1 BC007529 mRNA Translation: AAH07529.1 BC013985 mRNA Translation: AAH13985.1 U80745 mRNA Translation: AAB91443.1 |
CCDSi | CCDS13781.1 [Q96RN5-2] CCDS33602.1 [Q96RN5-1] CCDS74824.1 [Q96RN5-3] |
RefSeqi | NP_001003891.1, NM_001003891.2 [Q96RN5-1] NP_001280164.1, NM_001293235.1 NP_001280165.1, NM_001293236.1 [Q96RN5-3] NP_056973.2, NM_015889.4 [Q96RN5-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2GUT | NMR | - | A | 5-78 | [»] | |
BMRBi | Q96RN5 | |||||
SMRi | Q96RN5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119623, 94 interactors |
ComplexPortali | CPX-3227, Core mediator complex |
CORUMi | Q96RN5 |
DIPi | DIP-32908N |
IntActi | Q96RN5, 43 interactors |
MINTi | Q96RN5 |
STRINGi | 9606.ENSP00000263205 |
PTM databases
iPTMneti | Q96RN5 |
PhosphoSitePlusi | Q96RN5 |
Polymorphism and mutation databases
BioMutai | MED15 |
DMDMi | 27734440 |
Proteomic databases
EPDi | Q96RN5 |
jPOSTi | Q96RN5 |
MassIVEi | Q96RN5 |
MaxQBi | Q96RN5 |
PaxDbi | Q96RN5 |
PeptideAtlasi | Q96RN5 |
PRIDEi | Q96RN5 |
ProteomicsDBi | 77993 [Q96RN5-1] 77994 [Q96RN5-2] 77995 [Q96RN5-3] |
Protocols and materials databases
Antibodypediai | 282, 258 antibodies |
DNASUi | 51586 |
Genome annotation databases
Ensembli | ENST00000263205; ENSP00000263205; ENSG00000099917 [Q96RN5-1] ENST00000292733; ENSP00000292733; ENSG00000099917 [Q96RN5-2] ENST00000382974; ENSP00000372434; ENSG00000099917 [Q96RN5-3] |
GeneIDi | 51586 |
KEGGi | hsa:51586 |
UCSCi | uc002zsp.4, human [Q96RN5-1] |
Organism-specific databases
CTDi | 51586 |
DisGeNETi | 51586 |
EuPathDBi | HostDB:ENSG00000099917.17 |
GeneCardsi | MED15 |
HGNCi | HGNC:14248, MED15 |
HPAi | ENSG00000099917, Low tissue specificity |
MIMi | 607372, gene |
neXtProti | NX_Q96RN5 |
OpenTargetsi | ENSG00000099917 |
PharmGKBi | PA33088 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4274, Eukaryota |
GeneTreei | ENSGT00730000111140 |
HOGENOMi | CLU_023921_0_0_1 |
InParanoidi | Q96RN5 |
OMAi | QQNQMGM |
PhylomeDBi | Q96RN5 |
TreeFami | TF324988 |
Enzyme and pathway databases
PathwayCommonsi | Q96RN5 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-212436, Generic Transcription Pathway R-HSA-381340, Transcriptional regulation of white adipocyte differentiation |
Miscellaneous databases
BioGRID-ORCSi | 51586, 109 hits in 865 CRISPR screens |
ChiTaRSi | MED15, human |
EvolutionaryTracei | Q96RN5 |
GeneWikii | MED15 |
GenomeRNAii | 51586 |
Pharosi | Q96RN5, Tbio |
PROi | PR:Q96RN5 |
RNActi | Q96RN5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000099917, Expressed in sural nerve and 224 other tissues |
ExpressionAtlasi | Q96RN5, baseline and differential |
Genevisiblei | Q96RN5, HS |
Family and domain databases
Gene3Di | 1.10.246.20, 1 hit |
InterProi | View protein in InterPro IPR036529, KIX_dom_sf IPR019087, Med15 |
Pfami | View protein in Pfam PF09606, Med15, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MED15_HUMAN | |
Accessioni | Q96RN5Primary (citable) accession number: Q96RN5 Secondary accession number(s): D3DX31 Q9P1T3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2002 |
Last sequence update: | January 10, 2003 | |
Last modified: | December 2, 2020 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations