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Protein

Testis anion transporter 1

Gene

SLC26A8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a DIDS-sensitive anion exchanger mediating chloride, sulfate and oxalate transport. May fulfill critical anion exchange functions in male germ line during meiosis and hence may play a role in spermatogenesis. May be involved in a new regulatory pathway linking sulfate transport to RhoGTPase signaling in male germ cells. A critical component of the sperm annulus that is essential for correct sperm tail differentiation and motility and hence male fertility. May form a molecular complex involved in the regulation of chloride and bicarbonate ions fluxes during sperm capacitation.3 Publications

Activity regulationi

Activity is inhibited by 4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS - an inhibitor of several anion channels and transporters), gluconate, and by thiosulfate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processAnion exchange, Differentiation, Ion transport, Meiosis, Spermatogenesis, Transport

Protein family/group databases

TCDBi2.A.53.2.14 the sulfate permease (sulp) family

Names & Taxonomyi

Protein namesi
Recommended name:
Testis anion transporter 1
Alternative name(s):
Anion exchange transporter
Solute carrier family 26 member 8
Gene namesi
Name:SLC26A8Imported
Synonyms:TAT11 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000112053.13
HGNCiHGNC:14468 SLC26A8
MIMi608480 gene
neXtProtiNX_Q96RN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 95CytoplasmicSequence analysisAdd BLAST95
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 119ExtracellularSequence analysis3
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Topological domaini141 – 146CytoplasmicSequence analysis6
Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
Topological domaini168 – 202ExtracellularSequence analysisAdd BLAST35
Transmembranei203 – 223HelicalSequence analysisAdd BLAST21
Topological domaini224 – 232CytoplasmicSequence analysis9
Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
Topological domaini254 – 270ExtracellularSequence analysisAdd BLAST17
Transmembranei271 – 291HelicalSequence analysisAdd BLAST21
Topological domaini292 – 307CytoplasmicSequence analysisAdd BLAST16
Transmembranei308 – 328HelicalSequence analysisAdd BLAST21
Topological domaini329 – 355ExtracellularSequence analysisAdd BLAST27
Transmembranei356 – 376HelicalSequence analysisAdd BLAST21
Topological domaini377 – 392CytoplasmicSequence analysisAdd BLAST16
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 429ExtracellularSequence analysisAdd BLAST16
Transmembranei430 – 450HelicalSequence analysisAdd BLAST21
Topological domaini451 – 452CytoplasmicSequence analysis2
Transmembranei453 – 473HelicalSequence analysisAdd BLAST21
Topological domaini474 – 497ExtracellularSequence analysisAdd BLAST24
Transmembranei498 – 518HelicalSequence analysisAdd BLAST21
Topological domaini519 – 970CytoplasmicSequence analysisAdd BLAST452

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Spermatogenic failure 3 (SPGF3)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder characterized by primary infertility, sperm morphologic abnormalities, and moderate to severe asthenozoospermia, condition in which the percentage of progressively motile sperm is abnormally low.
See also OMIM:606766
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07005887R → Q in SPGF3; there is a reduced interactions with CFTR and complete failure to activate CFTR-dependent anion transport. 1 PublicationCorresponds to variant dbSNP:rs140210148EnsemblClinVar.1
Natural variantiVAR_070059812E → K in SPGF3; there is a reduced interactions with CFTR and complete failure to activate CFTR-dependent anion transport. 1 PublicationCorresponds to variant dbSNP:rs142724470EnsemblClinVar.1
Natural variantiVAR_070060954R → C in SPGF3; there is a reduced interactions with CFTR and complete failure to activate CFTR-dependent anion transport. 1 PublicationCorresponds to variant dbSNP:rs398123027EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi914P → S: Not a cause of male infertility. 1 Publication1

Organism-specific databases

DisGeNETi116369
MalaCardsiSLC26A8
MIMi606766 phenotype
OpenTargetsiENSG00000112053
Orphaneti276234 Non-syndromic male infertility due to sperm motility disorder
PharmGKBiPA37885

Polymorphism and mutation databases

BioMutaiSLC26A8
DMDMi74761075

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003225861 – 970Testis anion transporter 1Add BLAST970

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi192N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ96RN1
PaxDbiQ96RN1
PeptideAtlasiQ96RN1
PRIDEiQ96RN1
ProteomicsDBi77989
77990 [Q96RN1-2]
77991 [Q96RN1-3]
77992 [Q96RN1-4]

PTM databases

iPTMnetiQ96RN1
PhosphoSitePlusiQ96RN1

Expressioni

Tissue specificityi

Expression observed exclusively in testis, restricted to the meiotic phase of the germ cell. Abundant expression located in the seminiferous tubules, concentrated on the luminal side of the tubuli harboring the spermatocytes and spermatids. Expressed in spermatozoa.2 Publications

Inductioni

Repressed by tunicamycin, an inhibitor of N-glycosylation.1 Publication

Gene expression databases

BgeeiENSG00000112053 Expressed in 91 organ(s), highest expression level in testis
CleanExiHS_SLC26A8
ExpressionAtlasiQ96RN1 baseline and differential
GenevisibleiQ96RN1 HS

Organism-specific databases

HPAiHPA038081

Interactioni

Subunit structurei

Interacts with RACGAP1. Interacts with CFTR.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi125501, 2 interactors
IntActiQ96RN1, 2 interactors
STRINGi9606.ENSP00000347778

Structurei

3D structure databases

ProteinModelPortaliQ96RN1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini543 – 795STASPROSITE-ProRule annotationAdd BLAST253

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni664 – 970Interaction with RACGAP11 PublicationAdd BLAST307

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0236 Eukaryota
COG0659 LUCA
GeneTreeiENSGT00760000119026
HOGENOMiHOG000154234
HOVERGENiHBG108443
InParanoidiQ96RN1
KOiK14705
OMAiKIFQCCN
OrthoDBiEOG091G025O
PhylomeDBiQ96RN1
TreeFamiTF313784

Family and domain databases

InterProiView protein in InterPro
IPR011547 SLC26A/SulP_dom
IPR001902 SLC26A/SulP_fam
IPR030303 SLC26A8
IPR002645 STAS_dom
IPR036513 STAS_dom_sf
PANTHERiPTHR11814 PTHR11814, 1 hit
PTHR11814:SF11 PTHR11814:SF11, 1 hit
PfamiView protein in Pfam
PF01740 STAS, 1 hit
PF00916 Sulfate_transp, 1 hit
SUPFAMiSSF52091 SSF52091, 1 hit
PROSITEiView protein in PROSITE
PS50801 STAS, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 13 Publications (identifier: Q96RN1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAQLERSAIS GFSSKSRRNS FAYDVKREVY NEETFQQEHK RKASSSGNMN
60 70 80 90 100
INITTFRHHV QCRCSWHRFL RCVLTIFPFL EWMCMYRLKD WLLGDLLAGI
110 120 130 140 150
SVGLVQVPQG LTLSLLARQL IPPLNIAYAA FCSSVIYVIF GSCHQMSIGS
160 170 180 190 200
FFLVSALLIN VLKVSPFNNG QLVMGSFVKN EFSAPSYLMG YNKSLSVVAT
210 220 230 240 250
TTFLTGIIQL IMGVLGLGFI ATYLPESAMS AYLAAVALHI MLSQLTFIFG
260 270 280 290 300
IMISFHAGPI SFFYDIINYC VALPKANSTS ILVFLTVVVA LRINKCIRIS
310 320 330 340 350
FNQYPIEFPM ELFLIIGFTV IANKISMATE TSQTLIDMIP YSFLLPVTPD
360 370 380 390 400
FSLLPKIILQ AFSLSLVSSF LLIFLGKKIA SLHNYSVNSN QDLIAIGLCN
410 420 430 440 450
VVSSFFRSCV FTGAIARTII QDKSGGRQQF ASLVGAGVML LLMVKMGHFF
460 470 480 490 500
YTLPNAVLAG IILSNVIPYL ETISNLPSLW RQDQYDCALW MMTFSSSIFL
510 520 530 540 550
GLDIGLIISV VSAFFITTVR SHRAKILLLG QIPNTNIYRS INDYREIITI
560 570 580 590 600
PGVKIFQCCS SITFVNVYYL KHKLLKEVDM VKVPLKEEEI FSLFNSSDTN
610 620 630 640 650
LQGGKICRCF CNCDDLEPLP RILYTERFEN KLDPEASSIN LIHCSHFESM
660 670 680 690 700
NTSQTASEDQ VPYTVSSVSQ KNQGQQYEEV EEVWLPNNSS RNSSPGLPDV
710 720 730 740 750
AESQGRRSLI PYSDASLLPS VHTIILDFSM VHYVDSRGLV VLRQICNAFQ
760 770 780 790 800
NANILILIAG CHSSIVRAFE RNDFFDAGIT KTQLFLSVHD AVLFALSRKV
810 820 830 840 850
IGSSELSIDE SETVIRETYS ETDKNDNSRY KMSSSFLGSQ KNVSPGFIKI
860 870 880 890 900
QQPVEEESEL DLELESEQEA GLGLDLDLDR ELEPEMEPKA ETETKTQTEM
910 920 930 940 950
EPQPETEPEM EPNPKSRPRA HTFPQQRYWP MYHPSMASTQ SQTQTRTWSV
960 970
ERRRHPMDSY SPEGNSNEDV
Length:970
Mass (Da):109,006
Last modified:December 1, 2001 - v1
Checksum:i7F932A9CFE3A9EC4
GO
Isoform 21 Publication (identifier: Q96RN1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     210-314: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:865
Mass (Da):97,353
Checksum:i95925D014048905D
GO
Isoform 31 Publication (identifier: Q96RN1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-419: Missing.
     420-428: IQDKSGGRQ → VSLQLALSP

Note: No experimental confirmation available.Curated
Show »
Length:552
Mass (Da):62,455
Checksum:iFBB6C75D929BB281
GO
Isoform 41 Publication (identifier: Q96RN1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     745-768: ICNAFQNANILILIAGCHSSIVRA → VSTEEALAGALIPLLPSQPHPDPD
     769-970: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:768
Mass (Da):85,680
Checksum:i2A3BD6BC6188401F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7C4T4H7C4T4_HUMAN
Testis anion transporter 1
SLC26A8
320Annotation score:
C9JMV8C9JMV8_HUMAN
Testis anion transporter 1
SLC26A8
190Annotation score:
H7C5E6H7C5E6_HUMAN
Testis anion transporter 1
SLC26A8
184Annotation score:

Sequence cautioni

The sequence AAO26699 differs from that shown. Incorrectly indicated as originating from mouse.Curated
The sequence BAB71408 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti168N → D in AAL26868 (Ref. 3) Curated1
Sequence conflicti168N → D in AAO26699 (Ref. 3) Curated1
Sequence conflicti560S → C in AAL26868 (Ref. 3) Curated1
Sequence conflicti560S → C in AAO26699 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03946473V → M Not a cause of male infertility. 4 PublicationsCorresponds to variant dbSNP:rs743923Ensembl.1
Natural variantiVAR_07005887R → Q in SPGF3; there is a reduced interactions with CFTR and complete failure to activate CFTR-dependent anion transport. 1 PublicationCorresponds to variant dbSNP:rs140210148EnsemblClinVar.1
Natural variantiVAR_039465148I → V Not a cause of male infertility. 2 PublicationsCorresponds to variant dbSNP:rs17713154Ensembl.1
Natural variantiVAR_039466230S → N Not a cause of male infertility. 2 PublicationsCorresponds to variant dbSNP:rs17707331Ensembl.1
Natural variantiVAR_039467639I → V Not a cause of male infertility. 4 PublicationsCorresponds to variant dbSNP:rs2295852Ensembl.1
Natural variantiVAR_070059812E → K in SPGF3; there is a reduced interactions with CFTR and complete failure to activate CFTR-dependent anion transport. 1 PublicationCorresponds to variant dbSNP:rs142724470EnsemblClinVar.1
Natural variantiVAR_070060954R → C in SPGF3; there is a reduced interactions with CFTR and complete failure to activate CFTR-dependent anion transport. 1 PublicationCorresponds to variant dbSNP:rs398123027EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0527042 – 419Missing in isoform 3. 1 PublicationAdd BLAST418
Alternative sequenceiVSP_052705210 – 314Missing in isoform 2. 1 PublicationAdd BLAST105
Alternative sequenceiVSP_052706420 – 428IQDKSGGRQ → VSLQLALSP in isoform 3. 1 Publication9
Alternative sequenceiVSP_052707745 – 768ICNAF…SIVRA → VSTEEALAGALIPLLPSQPH PDPD in isoform 4. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_052708769 – 970Missing in isoform 4. 1 PublicationAdd BLAST202

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF331522 mRNA Translation: AAK95666.1
AF314959 mRNA Translation: AAL26868.1
AF403499 mRNA Translation: AAO26699.1
AL133507 Genomic DNA No translation available.
Z95152 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03862.1
BC025408 mRNA Translation: AAH25408.1
AK057276 mRNA Translation: BAB71408.1 Different initiation.
CCDSiCCDS4813.1 [Q96RN1-1]
CCDS4814.1 [Q96RN1-2]
RefSeqiNP_001180405.1, NM_001193476.1 [Q96RN1-1]
NP_443193.1, NM_052961.3 [Q96RN1-1]
NP_619732.2, NM_138718.2 [Q96RN1-2]
XP_016865724.1, XM_017010235.1 [Q96RN1-1]
UniGeneiHs.435836

Genome annotation databases

EnsembliENST00000355574; ENSP00000347778; ENSG00000112053 [Q96RN1-1]
ENST00000394602; ENSP00000378100; ENSG00000112053 [Q96RN1-2]
ENST00000490799; ENSP00000417638; ENSG00000112053 [Q96RN1-1]
GeneIDi116369
KEGGihsa:116369
UCSCiuc003oll.4 human [Q96RN1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF331522 mRNA Translation: AAK95666.1
AF314959 mRNA Translation: AAL26868.1
AF403499 mRNA Translation: AAO26699.1
AL133507 Genomic DNA No translation available.
Z95152 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03862.1
BC025408 mRNA Translation: AAH25408.1
AK057276 mRNA Translation: BAB71408.1 Different initiation.
CCDSiCCDS4813.1 [Q96RN1-1]
CCDS4814.1 [Q96RN1-2]
RefSeqiNP_001180405.1, NM_001193476.1 [Q96RN1-1]
NP_443193.1, NM_052961.3 [Q96RN1-1]
NP_619732.2, NM_138718.2 [Q96RN1-2]
XP_016865724.1, XM_017010235.1 [Q96RN1-1]
UniGeneiHs.435836

3D structure databases

ProteinModelPortaliQ96RN1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125501, 2 interactors
IntActiQ96RN1, 2 interactors
STRINGi9606.ENSP00000347778

Protein family/group databases

TCDBi2.A.53.2.14 the sulfate permease (sulp) family

PTM databases

iPTMnetiQ96RN1
PhosphoSitePlusiQ96RN1

Polymorphism and mutation databases

BioMutaiSLC26A8
DMDMi74761075

Proteomic databases

EPDiQ96RN1
PaxDbiQ96RN1
PeptideAtlasiQ96RN1
PRIDEiQ96RN1
ProteomicsDBi77989
77990 [Q96RN1-2]
77991 [Q96RN1-3]
77992 [Q96RN1-4]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355574; ENSP00000347778; ENSG00000112053 [Q96RN1-1]
ENST00000394602; ENSP00000378100; ENSG00000112053 [Q96RN1-2]
ENST00000490799; ENSP00000417638; ENSG00000112053 [Q96RN1-1]
GeneIDi116369
KEGGihsa:116369
UCSCiuc003oll.4 human [Q96RN1-1]

Organism-specific databases

CTDi116369
DisGeNETi116369
EuPathDBiHostDB:ENSG00000112053.13
GeneCardsiSLC26A8
HGNCiHGNC:14468 SLC26A8
HPAiHPA038081
MalaCardsiSLC26A8
MIMi606766 phenotype
608480 gene
neXtProtiNX_Q96RN1
OpenTargetsiENSG00000112053
Orphaneti276234 Non-syndromic male infertility due to sperm motility disorder
PharmGKBiPA37885
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0236 Eukaryota
COG0659 LUCA
GeneTreeiENSGT00760000119026
HOGENOMiHOG000154234
HOVERGENiHBG108443
InParanoidiQ96RN1
KOiK14705
OMAiKIFQCCN
OrthoDBiEOG091G025O
PhylomeDBiQ96RN1
TreeFamiTF313784

Miscellaneous databases

ChiTaRSiSLC26A8 human
GeneWikiiSLC26A8
GenomeRNAii116369
PROiPR:Q96RN1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112053 Expressed in 91 organ(s), highest expression level in testis
CleanExiHS_SLC26A8
ExpressionAtlasiQ96RN1 baseline and differential
GenevisibleiQ96RN1 HS

Family and domain databases

InterProiView protein in InterPro
IPR011547 SLC26A/SulP_dom
IPR001902 SLC26A/SulP_fam
IPR030303 SLC26A8
IPR002645 STAS_dom
IPR036513 STAS_dom_sf
PANTHERiPTHR11814 PTHR11814, 1 hit
PTHR11814:SF11 PTHR11814:SF11, 1 hit
PfamiView protein in Pfam
PF01740 STAS, 1 hit
PF00916 Sulfate_transp, 1 hit
SUPFAMiSSF52091 SSF52091, 1 hit
PROSITEiView protein in PROSITE
PS50801 STAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiS26A8_HUMAN
AccessioniPrimary (citable) accession number: Q96RN1
Secondary accession number(s): Q5JVR5
, Q812C7, Q8TC65, Q96MA0, Q96PK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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