UniProtKB - Q96QR8 (PURB_HUMAN)
Protein
Transcriptional activator protein Pur-beta
Gene
PURB
Organism
Homo sapiens (Human)
Status
Functioni
Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity).By similarity1 Publication
Miscellaneous
Defects in PURB may be a cause of progression of myelodysplastic syndrome (MDS) towards acute myelogenous leukemia (AML). MDS refers to a heterogeneous group of closely related hematopoietic disorders. All are characterized by a cellular marrow with impaired morphology and maturation (dysmyelopoiesis) and peripheral blood cytopenias, resulting from ineffective blood cell production. Some patients with MDS develop acute myelogenous leukemia (AML), a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 28 – 254 | By similarityAdd BLAST | 227 |
GO - Molecular functioni
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: GO_Central
- double-stranded DNA binding Source: GO_Central
- mRNA binding Source: UniProtKB
- purine-rich negative regulatory element binding Source: GO_Central
- RNA binding Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: GO_Central
- single-stranded DNA binding Source: UniProtKB
- SMAD binding Source: Ensembl
- transcription factor binding Source: UniProtKB
GO - Biological processi
- cell differentiation Source: InterPro
- cell population proliferation Source: InterPro
- regulation of myeloid cell differentiation Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q96QR8 |
SIGNORi | Q96QR8 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional activator protein Pur-betaAlternative name(s): Purine-rich element-binding protein B |
Gene namesi | Name:PURB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000146676.6 |
HGNCi | HGNC:9702, PURB |
MIMi | 608887, gene |
neXtProti | NX_Q96QR8 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 5814 |
OpenTargetsi | ENSG00000146676 |
PharmGKBi | PA34046 |
Miscellaneous databases
Pharosi | Q96QR8, Tbio |
Polymorphism and mutation databases
BioMutai | PURB |
DMDMi | 74732688 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000225615 | 2 – 312 | Transcriptional activator protein Pur-betaAdd BLAST | 311 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 6 | PhosphoserineCombined sources | 1 | |
Modified residuei | 8 | PhosphoserineCombined sources | 1 | |
Modified residuei | 24 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 31 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 101 | PhosphoserineCombined sources | 1 | |
Modified residuei | 152 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 267 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 294 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 298 | PhosphoserineCombined sources | 1 | |
Modified residuei | 304 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | Q96QR8 |
jPOSTi | Q96QR8 |
MassIVEi | Q96QR8 |
MaxQBi | Q96QR8 |
PaxDbi | Q96QR8 |
PeptideAtlasi | Q96QR8 |
PRIDEi | Q96QR8 |
ProteomicsDBi | 77888 |
PTM databases
iPTMneti | Q96QR8 |
PhosphoSitePlusi | Q96QR8 |
Expressioni
Tissue specificityi
Expressed in myocardium of heart failure patients.1 Publication
Gene expression databases
Bgeei | ENSG00000146676, Expressed in metanephros and 224 other tissues |
Genevisiblei | Q96QR8, HS |
Organism-specific databases
HPAi | ENSG00000146676, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer, heterodimer with PURA and heterotrimer with PURA and YBX1/Y-box protein 1.
By similarityBinary interactionsi
Hide detailsQ96QR8
GO - Molecular functioni
- SMAD binding Source: Ensembl
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 111773, 69 interactors |
IntActi | Q96QR8, 24 interactors |
STRINGi | 9606.ENSP00000379051 |
Miscellaneous databases
RNActi | Q96QR8, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 153 – 222 | Gly-richAdd BLAST | 70 |
Sequence similaritiesi
Belongs to the PUR DNA-binding protein family.Curated
Phylogenomic databases
eggNOGi | KOG3074, Eukaryota |
GeneTreei | ENSGT00950000183162 |
HOGENOMi | CLU_057873_1_1_1 |
InParanoidi | Q96QR8 |
OMAi | KMYERRD |
OrthoDBi | 1248813at2759 |
PhylomeDBi | Q96QR8 |
TreeFami | TF313701 |
Family and domain databases
InterProi | View protein in InterPro IPR006628, PUR-bd_fam IPR030499, PURbeta |
PANTHERi | PTHR12611, PTHR12611, 1 hit PTHR12611:SF4, PTHR12611:SF4, 1 hit |
Pfami | View protein in Pfam PF04845, PurA, 1 hit |
SMARTi | View protein in SMART SM00712, PUR, 3 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q96QR8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADGDSGSER GGGGGPCGFQ PASRGGGEQE TQELASKRLD IQNKRFYLDV
60 70 80 90 100
KQNAKGRFLK IAEVGAGGSK SRLTLSMAVA AEFRDSLGDF IEHYAQLGPS
110 120 130 140 150
SPEQLAAGAE EGGGPRRALK SEFLVRENRK YYLDLKENQR GRFLRIRQTV
160 170 180 190 200
NRGGGGFGAG PGPGGLQSGQ TIALPAQGLI EFRDALAKLI DDYGGEDDEL
210 220 230 240 250
AGGPGGGAGG PGGGLYGELP EGTSITVDSK RFFFDVGCNK YGVFLRVSEV
260 270 280 290 300
KPSYRNAITV PFKAWGKFGG AFCRYADEMK EIQERQRDKL YERRGGGSGG
310
GEESEGEEVD ED
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY039216 mRNA Translation: AAK72462.1 AC004854 Genomic DNA Translation: AAS00366.1 CH236960 Genomic DNA Translation: EAL23749.1 CH471128 Genomic DNA Translation: EAW61073.1 BC101735 mRNA Translation: AAI01736.1 BC101737 mRNA Translation: AAI01738.1 |
CCDSi | CCDS5499.1 |
PIRi | B45036 |
RefSeqi | NP_150093.1, NM_033224.4 |
Genome annotation databases
Ensembli | ENST00000395699; ENSP00000379051; ENSG00000146676 |
GeneIDi | 5814 |
KEGGi | hsa:5814 |
UCSCi | uc003tme.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY039216 mRNA Translation: AAK72462.1 AC004854 Genomic DNA Translation: AAS00366.1 CH236960 Genomic DNA Translation: EAL23749.1 CH471128 Genomic DNA Translation: EAW61073.1 BC101735 mRNA Translation: AAI01736.1 BC101737 mRNA Translation: AAI01738.1 |
CCDSi | CCDS5499.1 |
PIRi | B45036 |
RefSeqi | NP_150093.1, NM_033224.4 |
3D structure databases
SMRi | Q96QR8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111773, 69 interactors |
IntActi | Q96QR8, 24 interactors |
STRINGi | 9606.ENSP00000379051 |
PTM databases
iPTMneti | Q96QR8 |
PhosphoSitePlusi | Q96QR8 |
Polymorphism and mutation databases
BioMutai | PURB |
DMDMi | 74732688 |
Proteomic databases
EPDi | Q96QR8 |
jPOSTi | Q96QR8 |
MassIVEi | Q96QR8 |
MaxQBi | Q96QR8 |
PaxDbi | Q96QR8 |
PeptideAtlasi | Q96QR8 |
PRIDEi | Q96QR8 |
ProteomicsDBi | 77888 |
Protocols and materials databases
Antibodypediai | 44598, 90 antibodies |
Genome annotation databases
Ensembli | ENST00000395699; ENSP00000379051; ENSG00000146676 |
GeneIDi | 5814 |
KEGGi | hsa:5814 |
UCSCi | uc003tme.5, human |
Organism-specific databases
CTDi | 5814 |
DisGeNETi | 5814 |
EuPathDBi | HostDB:ENSG00000146676.6 |
GeneCardsi | PURB |
HGNCi | HGNC:9702, PURB |
HPAi | ENSG00000146676, Low tissue specificity |
MIMi | 608887, gene |
neXtProti | NX_Q96QR8 |
OpenTargetsi | ENSG00000146676 |
PharmGKBi | PA34046 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3074, Eukaryota |
GeneTreei | ENSGT00950000183162 |
HOGENOMi | CLU_057873_1_1_1 |
InParanoidi | Q96QR8 |
OMAi | KMYERRD |
OrthoDBi | 1248813at2759 |
PhylomeDBi | Q96QR8 |
TreeFami | TF313701 |
Enzyme and pathway databases
PathwayCommonsi | Q96QR8 |
SIGNORi | Q96QR8 |
Miscellaneous databases
BioGRID-ORCSi | 5814, 6 hits in 848 CRISPR screens |
ChiTaRSi | PURB, human |
GeneWikii | PURB |
GenomeRNAii | 5814 |
Pharosi | Q96QR8, Tbio |
PROi | PR:Q96QR8 |
RNActi | Q96QR8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000146676, Expressed in metanephros and 224 other tissues |
Genevisiblei | Q96QR8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR006628, PUR-bd_fam IPR030499, PURbeta |
PANTHERi | PTHR12611, PTHR12611, 1 hit PTHR12611:SF4, PTHR12611:SF4, 1 hit |
Pfami | View protein in Pfam PF04845, PurA, 1 hit |
SMARTi | View protein in SMART SM00712, PUR, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PURB_HUMAN | |
Accessioni | Q96QR8Primary (citable) accession number: Q96QR8 Secondary accession number(s): A4D2L7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 7, 2006 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families