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Entry version 185 (16 Oct 2019)
Sequence version 2 (25 Nov 2002)
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Protein

Vacuolar protein sorting-associated protein 35

Gene

VPS35

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15078903, PubMed:15247922, PubMed:20164305). Required for endosomal localization of WASHC2C (PubMed:22070227, PubMed:28892079). Mediates the association of the CSC with the WASH complex via WASHC2 (PubMed:22070227, PubMed:24980502, PubMed:24819384). Required for the endosomal localization of TBC1D5 (PubMed:20923837).4 Publications9 Publications
(Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus.2 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processHost-virus interaction, Protein transport, Transport

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-3238698 WNT ligand biogenesis and trafficking

Protein family/group databases

Transport Classification Database

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TCDBi
9.A.3.1.1 the sorting nexin27 (snx27)-retromer assembly apparatus

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 35
Short name:
hVPS35
Alternative name(s):
Maternal-embryonic 3
Vesicle protein sorting 35
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:VPS351 PublicationImported
Synonyms:MEM3
ORF Names:TCCCTA00141
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 16

Organism-specific databases

Human Gene Nomenclature Database

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HGNCi
HGNC:13487 VPS35

Online Mendelian Inheritance in Man (OMIM)

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MIMi
601501 gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_Q96QK1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Parkinson disease 17 (PARK17)6 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal dominant, adult-onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_066659620D → N in PARK17; decreases interaction with WASHC2C, FKBP15 and the WASH complex; impairs recruitment of the WASH complex to endosomes; shows reduced retrograde transport of selective cargo between lysosomes and the Golgi apparatus; shows a progressive reduction in neurite length and branching. 6 PublicationsCorresponds to variant dbSNP:rs188286943EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi108L → P: Disrupts interaction with VPS26; no effect on interaction with VPS29. 1 Publication1
Mutagenesisi675H → R: Disrupts interaction with VPS29. Does not effect interaction with VPS26. 1 Publication1

Keywords - Diseasei

Neurodegeneration, Parkinson disease, Parkinsonism

Organism-specific databases

DisGeNET

More...
DisGeNETi
55737

GeneReviews a resource of expert-authored, peer-reviewed disease descriptions.

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GeneReviewsi
VPS35

MalaCards human disease database

More...
MalaCardsi
VPS35
MIMi614203 phenotype

Open Targets

More...
OpenTargetsi
ENSG00000069329

Orphanet; a database dedicated to information on rare diseases and orphan drugs

More...
Orphaneti
411602 Hereditary late-onset Parkinson disease

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...
PharmGKBi
PA37783

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...
Pharosi
Q96QK1

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

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ChEMBLi
CHEMBL2216744

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

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BioMutai
VPS35

Domain mapping of disease mutations (DMDM)

More...
DMDMi
25453321

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000658961 – 796Vacuolar protein sorting-associated protein 35Add BLAST796

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei7PhosphoserineCombined sources1
Modified residuei783PhosphoserineBy similarity1
Modified residuei791PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

The CPTAC Assay portal

More...
CPTACi
CPTAC-605

Encyclopedia of Proteome Dynamics

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EPDi
Q96QK1

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q96QK1

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
Q96QK1

MaxQB - The MaxQuant DataBase

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MaxQBi
Q96QK1

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q96QK1

PeptideAtlas

More...
PeptideAtlasi
Q96QK1

PRoteomics IDEntifications database

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PRIDEi
Q96QK1

ProteomicsDB: a multi-organism proteome resource

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ProteomicsDBi
77884

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
Q96QK1

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
Q96QK1

SwissPalm database of S-palmitoylation events

More...
SwissPalmi
Q96QK1

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000069329 Expressed in 98 organ(s), highest expression level in intestine

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q96QK1 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q96QK1 HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
HPA040802

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the heterotrimeric retromer cargo-selective complex (CSC), also decribed as vacuolar protein sorting subcomplex (VPS), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35 (PubMed:11102511, PubMed:28892079). The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform (By similarity). The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants. The originally described retromer complex (also called SNX-BAR retromer) is a pentamer containing the CSC and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. The CSC associates with SNX3 to form a SNX3-retromer complex. The CSC associates with SNX27, the WASH complex and the SNX-BAR subcomplex to form the SNX27-retromer complex (Probable).

Interacts with VPS26A, VPS26B, VPS29, SNX1, SNX2, IGF2R, SNX3, GOLPH3, LRRK2, SLC11A2, WASHC2A, WASHC2C, FKBP15, WASHC1, RAB7A, SNX27, WASHC5, EHD1 (PubMed:11102511, PubMed:15078903, PubMed:17868075PubMed:22070227, PubMed:19553991, PubMed:21725319, PubMed:22070227, PubMed:22513087, PubMed:23331060, PubMed:23563491, PubMed:23395371, PubMed:24344282, PubMed:24980502, PubMed:17891154, PubMed:19531583).

Interacts with MAGEL2; leading to recruitment of the TRIM27:MAGEL2 E3 ubiquitin ligase complex retromer-containing endosomes (PubMed:23452853).

Interacts with SORCS2 (By similarity).

2 PublicationsBy similarity15 Publications

(Microbial infection) Interacts with human papillomavirus 16 minor capsid protein L2 (via C-terminus); this interaction mediates the transport of the capsid from the early endosome to the Golgi apparatus.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
120855, 88 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
Q96QK1

Database of interacting proteins

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DIPi
DIP-29076N

Protein interaction database and analysis system

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IntActi
Q96QK1, 41 interactors

Molecular INTeraction database

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MINTi
Q96QK1

STRING: functional protein association networks

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STRINGi
9606.ENSP00000299138

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1796
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q96QK1

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

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PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q96QK1

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni25 – 44Interaction with SNX31 PublicationAdd BLAST20
Regioni205 – 215Interaction with SNX31 PublicationAdd BLAST11
Regioni438 – 796Interaction with SLC11A21 PublicationAdd BLAST359
Regioni500 – 693Interaction with IGF2R cytoplasmic domain1 PublicationAdd BLAST194

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the VPS35 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1107 Eukaryota
ENOG410XNXC LUCA

Ensembl GeneTree

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GeneTreei
ENSGT00390000007315

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q96QK1

KEGG Orthology (KO)

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KOi
K18468

Identification of Orthologs from Complete Genome Data

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OMAi
HLWWATP

Database of Orthologous Groups

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OrthoDBi
716326at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q96QK1

TreeFam database of animal gene trees

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TreeFami
TF105659

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
1.25.40.660, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR016024 ARM-type_fold
IPR005378 Vps35
IPR042491 Vps35_C

The PANTHER Classification System

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PANTHERi
PTHR11099 PTHR11099, 1 hit

Pfam protein domain database

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Pfami
View protein in Pfam
PF03635 Vps35, 1 hit

PIRSF; a whole-protein classification database

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PIRSFi
PIRSF009375 Retromer_Vps35, 1 hit

Superfamily database of structural and functional annotation

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SUPFAMi
SSF48371 SSF48371, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q96QK1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPTTQQSPQD EQEKLLDEAI QAVKVQSFQM KRCLDKNKLM DALKHASNML
60 70 80 90 100
GELRTSMLSP KSYYELYMAI SDELHYLEVY LTDEFAKGRK VADLYELVQY
110 120 130 140 150
AGNIIPRLYL LITVGVVYVK SFPQSRKDIL KDLVEMCRGV QHPLRGLFLR
160 170 180 190 200
NYLLQCTRNI LPDEGEPTDE ETTGDISDSM DFVLLNFAEM NKLWVRMQHQ
210 220 230 240 250
GHSRDREKRE RERQELRILV GTNLVRLSQL EGVNVERYKQ IVLTGILEQV
260 270 280 290 300
VNCRDALAQE YLMECIIQVF PDEFHLQTLN PFLRACAELH QNVNVKNIII
310 320 330 340 350
ALIDRLALFA HREDGPGIPA DIKLFDIFSQ QVATVIQSRQ DMPSEDVVSL
360 370 380 390 400
QVSLINLAMK CYPDRVDYVD KVLETTVEIF NKLNLEHIAT SSAVSKELTR
410 420 430 440 450
LLKIPVDTYN NILTVLKLKH FHPLFEYFDY ESRKSMSCYV LSNVLDYNTE
460 470 480 490 500
IVSQDQVDSI MNLVSTLIQD QPDQPVEDPD PEDFADEQSL VGRFIHLLRS
510 520 530 540 550
EDPDQQYLIL NTARKHFGAG GNQRIRFTLP PLVFAAYQLA FRYKENSKVD
560 570 580 590 600
DKWEKKCQKI FSFAHQTISA LIKAELAELP LRLFLQGALA AGEIGFENHE
610 620 630 640 650
TVAYEFMSQA FSLYEDEISD SKAQLAAITL IIGTFERMKC FSEENHEPLR
660 670 680 690 700
TQCALAASKL LKKPDQGRAV STCAHLFWSG RNTDKNGEEL HGGKRVMECL
710 720 730 740 750
KKALKIANQC MDPSLQVQLF IEILNRYIYF YEKENDAVTI QVLNQLIQKI
760 770 780 790
REDLPNLESS EETEQINKHF HNTLEHLRLR RESPESEGPI YEGLIL
Length:796
Mass (Da):91,707
Last modified:November 25, 2002 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i28D2DD1C6B920A0A
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BRJ7H3BRJ7_HUMAN
Vacuolar protein sorting-associated...
VPS35
48Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
I3L4S0I3L4S0_HUMAN
Vacuolar protein sorting-associated...
VPS35
41Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A1W2PP10A0A1W2PP10_HUMAN
Vacuolar protein sorting-associated...
VPS35
46Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A3B3ITD4A0A3B3ITD4_HUMAN
Vacuolar protein sorting-associated...
VPS35
47Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAG01989 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAA91137 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB14626 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti42A → S in CAB66822 (PubMed:11230166).Curated1
Sequence conflicti160I → T in BAB14626 (PubMed:14702039).Curated1
Sequence conflicti168T → P in AAF89953 (PubMed:11102511).Curated1
Sequence conflicti453S → F in AAH10362 (PubMed:15489334).Curated1
Sequence conflicti526R → G in BAA91790 (PubMed:14702039).Curated1
Sequence conflicti694K → E in BAA91790 (PubMed:14702039).Curated1
Sequence conflicti796L → H in BAA91137 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06665351G → S1 PublicationCorresponds to variant dbSNP:rs193077277EnsemblClinVar.1
Natural variantiVAR_06665457M → I1 PublicationCorresponds to variant dbSNP:rs183554824EnsemblClinVar.1
Natural variantiVAR_06665582T → R1 PublicationCorresponds to variant dbSNP:rs188245364Ensembl.1
Natural variantiVAR_066656241I → M Found in a patient with Parkinson disease. 1 PublicationCorresponds to variant dbSNP:rs192783364EnsemblClinVar.1
Natural variantiVAR_066657316P → S Found in a patient with Parkinson disease. 1 PublicationCorresponds to variant dbSNP:rs770029606EnsemblClinVar.1
Natural variantiVAR_080769469Q → P Found in a consanguineous family with intellectual disability; unknown pathological significance. 1 Publication1
Natural variantiVAR_066658524R → W Found in a patient with Parkinson disease. 1 PublicationCorresponds to variant dbSNP:rs184277092EnsemblClinVar.1
Natural variantiVAR_054046602V → D. Corresponds to variant dbSNP:rs34687100Ensembl.1
Natural variantiVAR_066659620D → N in PARK17; decreases interaction with WASHC2C, FKBP15 and the WASH complex; impairs recruitment of the WASH complex to endosomes; shows reduced retrograde transport of selective cargo between lysosomes and the Golgi apparatus; shows a progressive reduction in neurite length and branching. 6 PublicationsCorresponds to variant dbSNP:rs188286943EnsemblClinVar.1
Natural variantiVAR_066660737A → V1 PublicationCorresponds to variant dbSNP:rs749516404EnsemblClinVar.1
Natural variantiVAR_066661774L → M1 PublicationCorresponds to variant dbSNP:rs192419029EnsemblClinVar.1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AF191298 mRNA Translation: AAF02778.2
AF186382 mRNA Translation: AAG40619.1
AF175265 mRNA Translation: AAF89953.1
AF183418 mRNA Translation: AAG09687.1
AK001614 mRNA Translation: BAA91790.1
AK023650 mRNA Translation: BAB14626.1 Different initiation.
AK000395 mRNA Translation: BAA91137.1 Different initiation.
AL136888 mRNA Translation: CAB66822.1
AL512769 mRNA Translation: CAC21686.1
BC002414 mRNA Translation: AAH02414.1
BC010362 mRNA Translation: AAH10362.1
BC093036 mRNA Translation: AAH93036.1
AY007112 mRNA Translation: AAG01989.1 Different initiation.

The Consensus CDS (CCDS) project

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CCDSi
CCDS10721.1

Protein sequence database of the Protein Information Resource

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PIRi
JC7516

NCBI Reference Sequences

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RefSeqi
NP_060676.2, NM_018206.5

Genome annotation databases

Ensembl eukaryotic genome annotation project

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Ensembli
ENST00000299138; ENSP00000299138; ENSG00000069329

Database of genes from NCBI RefSeq genomes

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GeneIDi
55737

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
hsa:55737

UCSC genome browser

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UCSCi
uc002eef.5 human

Keywords - Coding sequence diversityi

Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF191298 mRNA Translation: AAF02778.2
AF186382 mRNA Translation: AAG40619.1
AF175265 mRNA Translation: AAF89953.1
AF183418 mRNA Translation: AAG09687.1
AK001614 mRNA Translation: BAA91790.1
AK023650 mRNA Translation: BAB14626.1 Different initiation.
AK000395 mRNA Translation: BAA91137.1 Different initiation.
AL136888 mRNA Translation: CAB66822.1
AL512769 mRNA Translation: CAC21686.1
BC002414 mRNA Translation: AAH02414.1
BC010362 mRNA Translation: AAH10362.1
BC093036 mRNA Translation: AAH93036.1
AY007112 mRNA Translation: AAG01989.1 Different initiation.
CCDSiCCDS10721.1
PIRiJC7516
RefSeqiNP_060676.2, NM_018206.5

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R17X-ray2.80C/D483-780[»]
5F0JX-ray2.70A14-470[»]
5F0KX-ray3.07A/B/C/D/E14-470[»]
5F0LX-ray3.20A14-470[»]
5F0MX-ray3.10A14-470[»]
5F0PX-ray2.78A14-470[»]
5OSHX-ray4.30B/E/H/K482-780[»]
5OSIX-ray2.52B/E/H/K471-781[»]
SMRiQ96QK1
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi120855, 88 interactors
CORUMiQ96QK1
DIPiDIP-29076N
IntActiQ96QK1, 41 interactors
MINTiQ96QK1
STRINGi9606.ENSP00000299138

Chemistry databases

ChEMBLiCHEMBL2216744

Protein family/group databases

TCDBi9.A.3.1.1 the sorting nexin27 (snx27)-retromer assembly apparatus

PTM databases

iPTMnetiQ96QK1
PhosphoSitePlusiQ96QK1
SwissPalmiQ96QK1

Polymorphism and mutation databases

BioMutaiVPS35
DMDMi25453321

Proteomic databases

CPTACiCPTAC-605
EPDiQ96QK1
jPOSTiQ96QK1
MassIVEiQ96QK1
MaxQBiQ96QK1
PaxDbiQ96QK1
PeptideAtlasiQ96QK1
PRIDEiQ96QK1
ProteomicsDBi77884

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
55737

Genome annotation databases

EnsembliENST00000299138; ENSP00000299138; ENSG00000069329
GeneIDi55737
KEGGihsa:55737
UCSCiuc002eef.5 human

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
55737
DisGeNETi55737

GeneCards: human genes, protein and diseases

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GeneCardsi
VPS35
GeneReviewsiVPS35
HGNCiHGNC:13487 VPS35
HPAiHPA040802
MalaCardsiVPS35
MIMi601501 gene
614203 phenotype
neXtProtiNX_Q96QK1
OpenTargetsiENSG00000069329
Orphaneti411602 Hereditary late-onset Parkinson disease
PharmGKBiPA37783

GenAtlas: human gene database

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GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1107 Eukaryota
ENOG410XNXC LUCA
GeneTreeiENSGT00390000007315
InParanoidiQ96QK1
KOiK18468
OMAiHLWWATP
OrthoDBi716326at2759
PhylomeDBiQ96QK1
TreeFamiTF105659

Enzyme and pathway databases

ReactomeiR-HSA-3238698 WNT ligand biogenesis and trafficking

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
VPS35 human
EvolutionaryTraceiQ96QK1

The Gene Wiki collection of pages on human genes and proteins

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GeneWikii
VPS35

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

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GenomeRNAii
55737
PharosiQ96QK1

Protein Ontology

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PROi
PR:Q96QK1

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000069329 Expressed in 98 organ(s), highest expression level in intestine
ExpressionAtlasiQ96QK1 baseline and differential
GenevisibleiQ96QK1 HS

Family and domain databases

Gene3Di1.25.40.660, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR005378 Vps35
IPR042491 Vps35_C
PANTHERiPTHR11099 PTHR11099, 1 hit
PfamiView protein in Pfam
PF03635 Vps35, 1 hit
PIRSFiPIRSF009375 Retromer_Vps35, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiVPS35_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q96QK1
Secondary accession number(s): Q561W2
, Q9H016, Q9H096, Q9H4P3, Q9H8J0, Q9NRS7, Q9NVG2, Q9NX80, Q9NZK2
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: October 16, 2019
This is version 185 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  6. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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