UniProtKB - Q96QF0 (RAB3I_HUMAN)
Rab-3A-interacting protein
RAB3IP
Functioni
Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B (PubMed:12221131, PubMed:26824392).
Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (PubMed:12221131, PubMed:26824392).
Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5 (PubMed:20937701, PubMed:26824392).
Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface (PubMed:12221131).
Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297).
4 PublicationsGO - Molecular functioni
- GTPase binding Source: Ensembl
- guanyl-nucleotide exchange factor activity Source: UniProtKB
- identical protein binding Source: IntAct
GO - Biological processi
- ciliary basal body-plasma membrane docking Source: Reactome
- cilium assembly Source: BHF-UCL
- exocytosis Source: GO_Central
- Golgi to plasma membrane transport Source: BHF-UCL
- negative regulation of filopodium assembly Source: Ensembl
- protein localization to organelle Source: BHF-UCL
- protein targeting to membrane Source: UniProtKB
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q96QF0 |
Reactomei | R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-5620916, VxPx cargo-targeting to cilium R-HSA-5620922, BBSome-mediated cargo-targeting to cilium R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
SignaLinki | Q96QF0 |
SIGNORi | Q96QF0 |
Names & Taxonomyi
Protein namesi | Recommended name: Rab-3A-interacting proteinShort name: Rab3A-interacting protein Alternative name(s): Rabin-3 SSX2-interacting protein |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16508, RAB3IP |
MIMi | 608686, gene |
neXtProti | NX_Q96QF0 |
VEuPathDBi | HostDB:ENSG00000127328 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Cytoskeleton
- cytoskeleton 1 Publication
- centrosome 1 Publication
Other locations
- lamellipodium
- Vesicle 1 Publication
Note: Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus. Activation of protein kinase C results in redistribution to the periphery of lamellipodia.1 Publication
Cytoskeleton
- centrosome Source: BHF-UCL
- ciliary basal body Source: BHF-UCL
- microtubule organizing center Source: UniProtKB-SubCell
Cytosol
- cytosol Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: Ensembl
Nucleus
- nucleus Source: UniProtKB
Other locations
- Golgi to plasma membrane transport vesicle Source: GO_Central
- lamellipodium Source: UniProtKB-SubCell
- perinuclear region of cytoplasm Source: Ensembl
- proximal dendrite Source: Ensembl
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 117177 |
OpenTargetsi | ENSG00000127328 |
PharmGKBi | PA34136 |
Miscellaneous databases
Pharosi | Q96QF0, Tbio |
Genetic variation databases
BioMutai | RAB3IP |
DMDMi | 71152025 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097144 | 1 – 476 | Rab-3A-interacting proteinAdd BLAST | 476 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 163 | PhosphoserineCombined sources | 1 | |
Modified residuei | 263 | PhosphoserineBy similarity | 1 | |
Modified residuei | 266 | PhosphoserineCombined sources | 1 | |
Modified residuei | 288 | PhosphoserineCombined sources | 1 | |
Modified residuei | 296 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q96QF0 |
jPOSTi | Q96QF0 |
MassIVEi | Q96QF0 |
MaxQBi | Q96QF0 |
PaxDbi | Q96QF0 |
PeptideAtlasi | Q96QF0 |
PRIDEi | Q96QF0 |
ProteomicsDBi | 77867 [Q96QF0-1] 77868 [Q96QF0-2] 77869 [Q96QF0-3] 77870 [Q96QF0-4] 77871 [Q96QF0-5] 77872 [Q96QF0-6] 77873 [Q96QF0-7] 77874 [Q96QF0-8] |
PTM databases
GlyGeni | Q96QF0, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96QF0 |
PhosphoSitePlusi | Q96QF0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000127328, Expressed in corpus callosum and 176 other tissues |
ExpressionAtlasi | Q96QF0, baseline and differential |
Genevisiblei | Q96QF0, HS |
Organism-specific databases
HPAi | ENSG00000127328, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with the N-terminal region of SSX2 (PubMed:12007189).
Interacts with the GDP-bound forms of RAB8A and RAB8B (PubMed:26824392, PubMed:12221131). The interaction with RAB8A is prevented by phosphorylation of RAB8A at 'Thr-72' (PubMed:26824392).
Interacts with the GDP-bound forms of RAB3A and RAB3D (PubMed:12221131).
Interacts with DCDC1 (PubMed:22159412, PubMed:12007189, PubMed:12221131, PubMed:26824392).
Interacts (via the N-terminal region) with TRAPPC14; this interaction mediates RAB3IP association with the TRAPP II complex (PubMed:31467083).
5 PublicationsBinary interactionsi
Q96QF0
Isoform 2 [Q96QF0-1]
With | #Exp. | IntAct |
---|---|---|
BBS1 [Q8NFJ9] | 2 | EBI-747860,EBI-1805484 |
RAB3IP - isoform 1 [Q96QF0-2]
Isoform 7 [Q96QF0-7]
GO - Molecular functioni
- GTPase binding Source: Ensembl
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 125566, 108 interactors |
DIPi | DIP-41703N |
IntActi | Q96QF0, 90 interactors |
MINTi | Q96QF0 |
STRINGi | 9606.ENSP00000447300 |
Miscellaneous databases
RNActi | Q96QF0, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q96QF0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 262 – 297 | DisorderedSequence analysisAdd BLAST | 36 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 165 – 260 | Sequence analysisAdd BLAST | 96 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4324, Eukaryota |
GeneTreei | ENSGT00940000157998 |
HOGENOMi | CLU_038204_2_0_1 |
InParanoidi | Q96QF0 |
OMAi | AFTMAND |
OrthoDBi | 619794at2759 |
PhylomeDBi | Q96QF0 |
TreeFami | TF313748 |
Family and domain databases
InterProi | View protein in InterPro IPR040351, RAB3IL/RAB3IP/Sec2 IPR009449, Sec2_N |
PANTHERi | PTHR14430, PTHR14430, 1 hit |
Pfami | View protein in Pfam PF06428, Sec2p, 1 hit |
s (8+)i Sequence
Sequence statusi: Complete.
This entry describes 8 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 8 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGLKKMKGLS YDEAFAMAND PLEGFHEVNL ASPTSPDLLG VYESGTQEQT
60 70 80 90 100
TSPSVIYRPH PSALSSVPIQ ANALDVSELP TQPVYSSPRR LNCAEISSIS
110 120 130 140 150
FHVTDPAPCS TSGVTAGLTK LTTRKDNYNA EREFLQGATI TEACDGSDDI
160 170 180 190 200
FGLSTDSLSR LRSPSVLEVR EKGYERLKEE LAKAQRELKL KDEECERLSK
210 220 230 240 250
VRDQLGQELE ELTASLFEEA HKMVREANIK QATAEKQLKE AQGKIDVLQA
260 270 280 290 300
EVAALKTLVL SSSPTSPTQE PLPGGKTPFK KGHTRNKSTS SAMSGSHQDL
310 320 330 340 350
SVIQPIVKDC KEADLSLYNE FRLWKDEPTM DRTCPFLDKI YQEDIFPCLT
360 370 380 390 400
FSKSELASAV LEAVENNTLS IEPVGLQPIR FVKASAVECG GPKKCALTGQ
410 420 430 440 450
SKSCKHRIKL GDSSNYYYIS PFCRYRITSV CNFFTYIRYI QQGLVKQQDV
460 470
DQMFWEVMQL RKEMSLAKLG YFKEEL
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VNX9 | F8VNX9_HUMAN | Rab-3A-interacting protein | RAB3IP | 165 | Annotation score: | ||
H0YI02 | H0YI02_HUMAN | Rab-3A-interacting protein | RAB3IP | 237 | Annotation score: | ||
J3KPG1 | J3KPG1_HUMAN | Rab-3A-interacting protein | RAB3IP | 369 | Annotation score: | ||
H0YI66 | H0YI66_HUMAN | Rab-3A-interacting protein | RAB3IP | 91 | Annotation score: | ||
F8VTR6 | F8VTR6_HUMAN | Rab-3A-interacting protein | RAB3IP | 167 | Annotation score: | ||
H0YD03 | H0YD03_HUMAN | Rab-3A-interacting protein | RAB3IP | 189 | Annotation score: | ||
H0YHP4 | H0YHP4_HUMAN | Rab-3A-interacting protein | RAB3IP | 21 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 164 | P → Q in AAH59358 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 286 | N → D in BAB15391 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 356 | Missing in BAB15391 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 464 | M → V in BAB15391 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_051756 | 1 – 222 | Missing in isoform 8. 2 PublicationsAdd BLAST | 222 | |
Alternative sequenceiVSP_051755 | 1 – 16 | Missing in isoform 1, isoform 3, isoform 5 and isoform 7. 3 PublicationsAdd BLAST | 16 | |
Alternative sequenceiVSP_051758 | 313 – 331 | ADLSL…EPTMD → VTHQGLSPLTLLILVSSHH in isoform 7. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_051757 | 313 – 320 | ADLSLYNE → KMCSHWPE in isoform 5 and isoform 6. 1 Publication | 8 | |
Alternative sequenceiVSP_051759 | 321 – 476 | Missing in isoform 5 and isoform 6. 1 PublicationAdd BLAST | 156 | |
Alternative sequenceiVSP_051760 | 332 – 476 | Missing in isoform 7. 1 PublicationAdd BLAST | 145 | |
Alternative sequenceiVSP_051761 | 394 – 411 | KCALT…RIKLG → SLLYVTFLHTFDTFSRDS in isoform 3 and isoform 4. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_051762 | 412 – 476 | Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST | 65 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ312896 mRNA Translation: CAC59835.1 AJ312897 mRNA Translation: CAC59836.1 AJ312898 mRNA Translation: CAC59837.1 AJ312899 mRNA Translation: CAC59838.1 AJ312900 mRNA Translation: CAC59839.1 AJ312901 mRNA Translation: CAC59840.1 AK026201 mRNA Translation: BAB15391.1 AK027566 mRNA Translation: BAB55202.1 AK027671 mRNA Translation: BAB55283.1 AC025263 Genomic DNA No translation available. BC015548 mRNA Translation: AAH15548.1 BC059358 mRNA Translation: AAH59358.1 |
CCDSi | CCDS41811.1 [Q96QF0-4] CCDS44942.1 [Q96QF0-8] CCDS8993.1 [Q96QF0-1] CCDS8995.1 [Q96QF0-2] CCDS8996.1 [Q96QF0-3] |
RefSeqi | NP_001019818.1, NM_001024647.3 [Q96QF0-8] NP_001265331.1, NM_001278402.1 [Q96QF0-8] NP_071901.2, NM_022456.4 [Q96QF0-2] NP_783322.1, NM_175623.3 [Q96QF0-1] NP_783323.1, NM_175624.3 [Q96QF0-3] NP_783324.1, NM_175625.3 [Q96QF0-4] XP_016874262.1, XM_017018773.1 [Q96QF0-2] XP_016874265.1, XM_017018776.1 XP_016874266.1, XM_017018777.1 [Q96QF0-8] |
Genome annotation databases
Ensembli | ENST00000247833; ENSP00000247833; ENSG00000127328 [Q96QF0-2] ENST00000362025; ENSP00000355381; ENSG00000127328 [Q96QF0-4] ENST00000378815; ENSP00000368092; ENSG00000127328 [Q96QF0-7] ENST00000417413; ENSP00000436304; ENSG00000127328 [Q96QF0-5] ENST00000483530; ENSP00000419216; ENSG00000127328 [Q96QF0-3] ENST00000550536; ENSP00000447300; ENSG00000127328 ENST00000551641; ENSP00000448773; ENSG00000127328 [Q96QF0-8] ENST00000552199; ENSP00000448944; ENSG00000127328 [Q96QF0-6] ENST00000553099; ENSP00000448027; ENSG00000127328 [Q96QF0-8] |
GeneIDi | 117177 |
KEGGi | hsa:117177 |
MANE-Selecti | ENST00000247833.12; ENSP00000247833.7; NM_022456.5; NP_071901.2 [Q96QF0-2] |
UCSCi | uc001svm.5, human [Q96QF0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ312896 mRNA Translation: CAC59835.1 AJ312897 mRNA Translation: CAC59836.1 AJ312898 mRNA Translation: CAC59837.1 AJ312899 mRNA Translation: CAC59838.1 AJ312900 mRNA Translation: CAC59839.1 AJ312901 mRNA Translation: CAC59840.1 AK026201 mRNA Translation: BAB15391.1 AK027566 mRNA Translation: BAB55202.1 AK027671 mRNA Translation: BAB55283.1 AC025263 Genomic DNA No translation available. BC015548 mRNA Translation: AAH15548.1 BC059358 mRNA Translation: AAH59358.1 |
CCDSi | CCDS41811.1 [Q96QF0-4] CCDS44942.1 [Q96QF0-8] CCDS8993.1 [Q96QF0-1] CCDS8995.1 [Q96QF0-2] CCDS8996.1 [Q96QF0-3] |
RefSeqi | NP_001019818.1, NM_001024647.3 [Q96QF0-8] NP_001265331.1, NM_001278402.1 [Q96QF0-8] NP_071901.2, NM_022456.4 [Q96QF0-2] NP_783322.1, NM_175623.3 [Q96QF0-1] NP_783323.1, NM_175624.3 [Q96QF0-3] NP_783324.1, NM_175625.3 [Q96QF0-4] XP_016874262.1, XM_017018773.1 [Q96QF0-2] XP_016874265.1, XM_017018776.1 XP_016874266.1, XM_017018777.1 [Q96QF0-8] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4LHX | X-ray | 3.05 | C/D/E/F | 173-248 | [»] | |
4LHY | X-ray | 3.10 | C/D/E/F | 173-248 | [»] | |
4LHZ | X-ray | 3.20 | C/D/E/F | 173-248 | [»] | |
4UJ3 | X-ray | 3.00 | B/E/H/K/N/Q/T/W | 286-476 | [»] | |
4UJ4 | X-ray | 4.20 | B/E/H/K | 286-476 | [»] | |
4UJ5 | X-ray | 2.60 | C/D | 286-476 | [»] | |
6F6P | X-ray | 2.45 | A/B/C/D | 159-261 | [»] | |
SMRi | Q96QF0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 125566, 108 interactors |
DIPi | DIP-41703N |
IntActi | Q96QF0, 90 interactors |
MINTi | Q96QF0 |
STRINGi | 9606.ENSP00000447300 |
PTM databases
GlyGeni | Q96QF0, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96QF0 |
PhosphoSitePlusi | Q96QF0 |
Genetic variation databases
BioMutai | RAB3IP |
DMDMi | 71152025 |
Proteomic databases
EPDi | Q96QF0 |
jPOSTi | Q96QF0 |
MassIVEi | Q96QF0 |
MaxQBi | Q96QF0 |
PaxDbi | Q96QF0 |
PeptideAtlasi | Q96QF0 |
PRIDEi | Q96QF0 |
ProteomicsDBi | 77867 [Q96QF0-1] 77868 [Q96QF0-2] 77869 [Q96QF0-3] 77870 [Q96QF0-4] 77871 [Q96QF0-5] 77872 [Q96QF0-6] 77873 [Q96QF0-7] 77874 [Q96QF0-8] |
Protocols and materials databases
Antibodypediai | 29437, 219 antibodies from 24 providers |
DNASUi | 117177 |
Genome annotation databases
Organism-specific databases
CTDi | 117177 |
DisGeNETi | 117177 |
GeneCardsi | RAB3IP |
HGNCi | HGNC:16508, RAB3IP |
HPAi | ENSG00000127328, Low tissue specificity |
MIMi | 608686, gene |
neXtProti | NX_Q96QF0 |
OpenTargetsi | ENSG00000127328 |
PharmGKBi | PA34136 |
VEuPathDBi | HostDB:ENSG00000127328 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4324, Eukaryota |
GeneTreei | ENSGT00940000157998 |
HOGENOMi | CLU_038204_2_0_1 |
InParanoidi | Q96QF0 |
OMAi | AFTMAND |
OrthoDBi | 619794at2759 |
PhylomeDBi | Q96QF0 |
TreeFami | TF313748 |
Enzyme and pathway databases
PathwayCommonsi | Q96QF0 |
Reactomei | R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-5620916, VxPx cargo-targeting to cilium R-HSA-5620922, BBSome-mediated cargo-targeting to cilium R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
SignaLinki | Q96QF0 |
SIGNORi | Q96QF0 |
Miscellaneous databases
BioGRID-ORCSi | 117177, 6 hits in 1047 CRISPR screens |
ChiTaRSi | RAB3IP, human |
GeneWikii | RAB3IP |
GenomeRNAii | 117177 |
Pharosi | Q96QF0, Tbio |
PROi | PR:Q96QF0 |
RNActi | Q96QF0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000127328, Expressed in corpus callosum and 176 other tissues |
ExpressionAtlasi | Q96QF0, baseline and differential |
Genevisiblei | Q96QF0, HS |
Family and domain databases
InterProi | View protein in InterPro IPR040351, RAB3IL/RAB3IP/Sec2 IPR009449, Sec2_N |
PANTHERi | PTHR14430, PTHR14430, 1 hit |
Pfami | View protein in Pfam PF06428, Sec2p, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAB3I_HUMAN | |
Accessioni | Q96QF0Primary (citable) accession number: Q96QF0 Secondary accession number(s): B7WPJ6 Q9H673 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families