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UniProtKB - Q96Q83 (ALKB3_HUMAN)

Entry version 167 (07 Apr 2021)
Sequence version 1 (01 Dec 2001)
Previous versions | rss
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Protein

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3

Gene

ALKBH3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N1-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410).8 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activated by ascorbate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei115SubstrateBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi191Iron; catalyticPROSITE-ProRule annotationCombined sources1 Publication1
Metal bindingi193Iron; catalyticPROSITE-ProRule annotationCombined sources1 Publication1
Binding sitei194SubstrateBy similarity1
Metal bindingi257Iron; catalyticPROSITE-ProRule annotationCombined sources1 Publication1
Binding sitei275Alpha-ketoglutarateCombined sources1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processDNA damage, DNA repair
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		1.14.11.33, 2681

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q96Q83

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-112126, ALKBH3 mediated reversal of alkylation damage

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3Curated (EC:1.14.11.541 Publication2 Publications)
Alternative name(s):
Alkylated DNA repair protein alkB homolog 31 Publication
Short name:
hABH31 Publication
DEPC-11 Publication
Prostate cancer antigen 11 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ALKBH3Imported
Synonyms:ABH31 Publication, DEPC11 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 11

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:30141, ALKBH3

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		610603, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q96Q83

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000166199.12

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi122R → A: Decreases activity towards ssDNA by 25%. Loss of activity towards dsDNA. 1
Mutagenesisi123E → A: Strongly increases activity towards dsDNA, possibly by facilitating access to the active site. 1
Mutagenesisi131R → A: Loss of activity. 1
Mutagenesisi177L → A or N: Loss of activity against 1-methyladenine. 1
Mutagenesisi177L → E or Q: Loss of activity. 1
Mutagenesisi177L → I: Decreases activity against 1-methyladenine. 1
Mutagenesisi177L → M: No effect. 1
Mutagenesisi179N → A: Decreases activity by about 60%. 1
Mutagenesisi181Y → A: Strong decrease of activity. 1
Mutagenesisi189D → A: Strongly increases activity towards dsDNA, possibly by facilitating access to the active site. 1
Mutagenesisi191H → A: Loss of activity. 1
Mutagenesisi193D → A: Loss of activity. 2 Publications1
Mutagenesisi257H → A: Decreases activity by about 65%. 2 Publications1
Mutagenesisi269R → A: Strong decrease of activity. 1
Mutagenesisi271N → A: No effect. 1
Mutagenesisi275R → A: Loss of activity. 1

Organism-specific databases

DisGeNET

More...DisGeNETi		221120

Open Targets

More...OpenTargetsi		ENSG00000166199

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA143485293

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q96Q83, Tchem

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL3112376

Drug and drug target database

More...DrugBanki		DB00126, Ascorbic acid

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		ALKBH3

Domain mapping of disease mutations (DMDM)

More...DMDMi		74752087

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002392781 – 286Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3Add BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei177(4R)-5-hydroxyleucine; alternate1 Publication1
Modified residuei177(4R)-5-oxoleucine; alternate1 Publication1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination. OTUD4 promotes USP7 and USP9X-dependent deubiquitination of 'Lys-48'-polyubiquitinated ALKBH3 promoting the repair of alkylated DNA lesions.1 Publication

Keywords - PTMi

Hydroxylation, Oxidation, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q96Q83

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q96Q83

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q96Q83

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q96Q83

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q96Q83

PeptideAtlas

More...PeptideAtlasi		Q96Q83

PRoteomics IDEntifications database

More...PRIDEi		Q96Q83

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		77836 [Q96Q83-1]
77837 [Q96Q83-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q96Q83

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q96Q83

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.3 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000166199, Expressed in skeletal muscle tissue and 187 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q96Q83, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q96Q83, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000166199, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with the ASCC complex composed of ASCC1, ASCC2 and ASCC3 (PubMed:29144457, PubMed:22055184).

Interacts directly with ASCC3, and is thereby recruited to the ASCC complex (PubMed:22055184, PubMed:29144457).

Interacts with OTUD4; the interaction is direct (PubMed:25944111).

Interacts with USP7 and USP9X (PubMed:25944111).

3 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		128686, 53 interactors

Protein interaction database and analysis system

More...IntActi		Q96Q83, 21 interactors

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000302232

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		Q96Q83

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q96Q83, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q96Q83

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		Q96Q83

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini172 – 278Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST107

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni141 – 143Substrate bindingBy similarity3
Regioni179 – 181Alpha-ketoglutarate bindingCombined sources1 Publication3
Regioni269 – 275Alpha-ketoglutarate bindingCombined sources1 Publication7

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the alkB family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG502QW9E, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000157226

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_048788_2_1_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q96Q83

Identification of Orthologs from Complete Genome Data

More...OMAi		TMQPNPN

Database of Orthologous Groups

More...OrthoDBi		1494495at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q96Q83

TreeFam database of animal gene trees

More...TreeFami		TF331732

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.60.120.590, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR027450, AlkB-like
IPR037151, AlkB-like_sf
IPR032854, ALKBH3
IPR005123, Oxoglu/Fe-dep_dioxygenase

The PANTHER Classification System

More...PANTHERi		PTHR31212, PTHR31212, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF13532, 2OG-FeII_Oxy_2, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51471, FE2OG_OXY, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96Q83-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEEKRRRARV QGAWAAPVKS QAIAQPATTA KSHLHQKPGQ TWKNKEHHLS 
        60         70         80         90        100
DREFVFKEPQ QVVRRAPEPR VIDREGVYEI SLSPTGVSRV CLYPGFVDVK 
       110        120        130        140        150
EADWILEQLC QDVPWKQRTG IREDITYQQP RLTAWYGELP YTYSRITMEP 
       160        170        180        190        200
NPHWHPVLRT LKNRIEENTG HTFNSLLCNL YRNEKDSVDW HSDDEPSLGR 
       210        220        230        240        250
CPIIASLSFG ATRTFEMRKK PPPEENGDYT YVERVKIPLD HGTLLIMEGA 
       260        270        280 
TQADWQHRVP KEYHSREPRV NLTFRTVYPD PRGAPW
Length:286
Mass (Da):33,375
Last modified:December 1, 2001 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF6563635B1F217D7
GO
Isoform 2 (identifier: Q96Q83-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     73-74: DR → E
     125-172: ITYQQPRLTA...NRIEENTGHT → SILQLTFKKS...ILTRTRLWAP
     173-286: Missing.

Show »
Length:170
Mass (Da):19,310
Checksum:i9BC42BA5B40B035C
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PSA5E9PSA5_HUMAN
Alpha-ketoglutarate-dependent dioxy...
ALKBH3
223Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0YDH4H0YDH4_HUMAN
Alpha-ketoglutarate-dependent dioxy...
ALKBH3
156Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9PN35E9PN35_HUMAN
Alpha-ketoglutarate-dependent dioxy...
ALKBH3
171Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9PRJ2E9PRJ2_HUMAN
Alpha-ketoglutarate-dependent dioxy...
ALKBH3
66Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9PRD0E9PRD0_HUMAN
Alpha-ketoglutarate-dependent dioxy...
ALKBH3
60Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAH15155 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_026632164R → C1 PublicationCorresponds to variant dbSNP:rs2271815Ensembl.1
Natural variantiVAR_026631228D → E2 PublicationsCorresponds to variant dbSNP:rs1130290Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_01912573 – 74DR → E in isoform 2. 1 Publication2
Alternative sequenceiVSP_019126125 – 172ITYQQ…NTGHT → SILQLTFKKSAPVSGTATAP QSCWYERPSPPHIPGPAILT RTRLWAP in isoform 2. 1 PublicationAdd BLAST48
Alternative sequenceiVSP_019127173 – 286Missing in isoform 2. 1 PublicationAdd BLAST114

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AB042029 mRNA Translation: BAB70508.1
DQ196343 Genomic DNA Translation: ABA27096.1
AC087521 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68078.1
BC015155 mRNA Translation: AAH15155.1 Different initiation.
BC103812 mRNA Translation: AAI03813.1
BC103813 mRNA Translation: AAI03814.1
BC103814 mRNA Translation: AAI03815.1
BC067257 mRNA Translation: AAH67257.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS7906.1 [Q96Q83-1]

NCBI Reference Sequences

More...RefSeqi		NP_631917.1, NM_139178.3 [Q96Q83-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000302708; ENSP00000302232; ENSG00000166199 [Q96Q83-1]
ENST00000530803; ENSP00000436788; ENSG00000166199 [Q96Q83-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		221120

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:221120

UCSC genome browser

More...UCSCi		uc001mxs.3, human [Q96Q83-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross%5Freferences%5Fsection">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042029 mRNA Translation: BAB70508.1
DQ196343 Genomic DNA Translation: ABA27096.1
AC087521 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68078.1
BC015155 mRNA Translation: AAH15155.1 Different initiation.
BC103812 mRNA Translation: AAI03813.1
BC103813 mRNA Translation: AAI03814.1
BC103814 mRNA Translation: AAI03815.1
BC067257 mRNA Translation: AAH67257.1
CCDSiCCDS7906.1 [Q96Q83-1]
RefSeqiNP_631917.1, NM_139178.3 [Q96Q83-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IUWX-ray1.50A70-286[»]
SMRiQ96Q83
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi128686, 53 interactors
IntActiQ96Q83, 21 interactors
STRINGi9606.ENSP00000302232

Chemistry databases

BindingDBiQ96Q83
ChEMBLiCHEMBL3112376
DrugBankiDB00126, Ascorbic acid

PTM databases

iPTMnetiQ96Q83
PhosphoSitePlusiQ96Q83

Genetic variation databases

BioMutaiALKBH3
DMDMi74752087

Proteomic databases

EPDiQ96Q83
jPOSTiQ96Q83
MassIVEiQ96Q83
MaxQBiQ96Q83
PaxDbiQ96Q83
PeptideAtlasiQ96Q83
PRIDEiQ96Q83
ProteomicsDBi77836 [Q96Q83-1]
77837 [Q96Q83-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		1876, 253 antibodies

Genome annotation databases

EnsembliENST00000302708; ENSP00000302232; ENSG00000166199 [Q96Q83-1]
ENST00000530803; ENSP00000436788; ENSG00000166199 [Q96Q83-2]
GeneIDi221120
KEGGihsa:221120
UCSCiuc001mxs.3, human [Q96Q83-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		221120
DisGeNETi221120

GeneCards: human genes, protein and diseases

More...GeneCardsi		ALKBH3
HGNCiHGNC:30141, ALKBH3
HPAiENSG00000166199, Low tissue specificity
MIMi610603, gene
neXtProtiNX_Q96Q83
OpenTargetsiENSG00000166199
PharmGKBiPA143485293
VEuPathDBiHostDB:ENSG00000166199.12

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiENOG502QW9E, Eukaryota
GeneTreeiENSGT00940000157226
HOGENOMiCLU_048788_2_1_1
InParanoidiQ96Q83
OMAiTMQPNPN
OrthoDBi1494495at2759
PhylomeDBiQ96Q83
TreeFamiTF331732

Enzyme and pathway databases

BRENDAi1.14.11.33, 2681
PathwayCommonsiQ96Q83
ReactomeiR-HSA-112126, ALKBH3 mediated reversal of alkylation damage

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		221120, 14 hits in 999 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		ALKBH3, human
EvolutionaryTraceiQ96Q83

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		221120
PharosiQ96Q83, Tchem

Protein Ontology

More...PROi		PR:Q96Q83
RNActiQ96Q83, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000166199, Expressed in skeletal muscle tissue and 187 other tissues
ExpressionAtlasiQ96Q83, baseline and differential
GenevisibleiQ96Q83, HS

Family and domain databases

Gene3Di2.60.120.590, 1 hit
InterProiView protein in InterPro
IPR027450, AlkB-like
IPR037151, AlkB-like_sf
IPR032854, ALKBH3
IPR005123, Oxoglu/Fe-dep_dioxygenase
PANTHERiPTHR31212, PTHR31212, 1 hit
PfamiView protein in Pfam
PF13532, 2OG-FeII_Oxy_2, 1 hit
PROSITEiView protein in PROSITE
PS51471, FE2OG_OXY, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiALKB3_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q96Q83
Secondary accession number(s): A6NDJ1
, Q3SYI0, Q6NX57, Q96BU8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: December 1, 2001
Last modified: April 7, 2021
This is version 167 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families
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