UniProtKB - Q96Q83 (ALKB3_HUMAN)
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
ALKBH3
Functioni
Catalytic activityi
- 2-oxoglutarate + N1-methyladenosine in mRNA + O2 = adenosine in mRNA + CO2 + formaldehyde + succinate1 PublicationEC:1.14.11.541 Publication
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 115 | SubstrateBy similarity | 1 | |
Metal bindingi | 191 | Iron; catalyticPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 193 | Iron; catalyticPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 194 | SubstrateBy similarity | 1 | |
Metal bindingi | 257 | Iron; catalyticPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 275 | Alpha-ketoglutarateCombined sources1 Publication | 1 |
GO - Molecular functioni
- DNA-N1-methyladenine dioxygenase activity Source: UniProtKB
- ferrous iron binding Source: UniProtKB
- L-ascorbic acid binding Source: UniProtKB-KW
- RNA N1-methyladenosine dioxygenase activity Source: UniProtKB
GO - Biological processi
- cell population proliferation Source: UniProtKB
- DNA dealkylation involved in DNA repair Source: UniProtKB
- DNA repair Source: UniProtKB
- oxidative single-stranded DNA demethylation Source: UniProtKB
- oxidative single-stranded RNA demethylation Source: UniProtKB
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | DNA damage, DNA repair |
Ligand | Iron, Metal-binding, Vitamin C |
Enzyme and pathway databases
BRENDAi | 1.14.11.33, 2681 |
PathwayCommonsi | Q96Q83 |
Reactomei | R-HSA-112126, ALKBH3 mediated reversal of alkylation damage |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3Curated (EC:1.14.11.541 Publication2 Publications)Alternative name(s): Alkylated DNA repair protein alkB homolog 31 Publication Short name: hABH31 Publication DEPC-11 Publication Prostate cancer antigen 11 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30141, ALKBH3 |
MIMi | 610603, gene |
neXtProti | NX_Q96Q83 |
VEuPathDBi | HostDB:ENSG00000166199.12 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Mitochondrion
- mitochondrion Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 122 | R → A: Decreases activity towards ssDNA by 25%. Loss of activity towards dsDNA. | 1 | |
Mutagenesisi | 123 | E → A: Strongly increases activity towards dsDNA, possibly by facilitating access to the active site. | 1 | |
Mutagenesisi | 131 | R → A: Loss of activity. | 1 | |
Mutagenesisi | 177 | L → A or N: Loss of activity against 1-methyladenine. | 1 | |
Mutagenesisi | 177 | L → E or Q: Loss of activity. | 1 | |
Mutagenesisi | 177 | L → I: Decreases activity against 1-methyladenine. | 1 | |
Mutagenesisi | 177 | L → M: No effect. | 1 | |
Mutagenesisi | 179 | N → A: Decreases activity by about 60%. | 1 | |
Mutagenesisi | 181 | Y → A: Strong decrease of activity. | 1 | |
Mutagenesisi | 189 | D → A: Strongly increases activity towards dsDNA, possibly by facilitating access to the active site. | 1 | |
Mutagenesisi | 191 | H → A: Loss of activity. | 1 | |
Mutagenesisi | 193 | D → A: Loss of activity. 2 Publications | 1 | |
Mutagenesisi | 257 | H → A: Decreases activity by about 65%. 2 Publications | 1 | |
Mutagenesisi | 269 | R → A: Strong decrease of activity. | 1 | |
Mutagenesisi | 271 | N → A: No effect. | 1 | |
Mutagenesisi | 275 | R → A: Loss of activity. | 1 |
Organism-specific databases
DisGeNETi | 221120 |
OpenTargetsi | ENSG00000166199 |
PharmGKBi | PA143485293 |
Miscellaneous databases
Pharosi | Q96Q83, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3112376 |
DrugBanki | DB00126, Ascorbic acid |
Genetic variation databases
BioMutai | ALKBH3 |
DMDMi | 74752087 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000239278 | 1 – 286 | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3Add BLAST | 286 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 177 | (4R)-5-hydroxyleucine; alternate1 Publication | 1 | |
Modified residuei | 177 | (4R)-5-oxoleucine; alternate1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Hydroxylation, Oxidation, Ubl conjugationProteomic databases
EPDi | Q96Q83 |
jPOSTi | Q96Q83 |
MassIVEi | Q96Q83 |
MaxQBi | Q96Q83 |
PaxDbi | Q96Q83 |
PeptideAtlasi | Q96Q83 |
PRIDEi | Q96Q83 |
ProteomicsDBi | 77836 [Q96Q83-1] 77837 [Q96Q83-2] |
PTM databases
iPTMneti | Q96Q83 |
PhosphoSitePlusi | Q96Q83 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000166199, Expressed in skeletal muscle tissue and 187 other tissues |
ExpressionAtlasi | Q96Q83, baseline and differential |
Genevisiblei | Q96Q83, HS |
Organism-specific databases
HPAi | ENSG00000166199, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with the ASCC complex composed of ASCC1, ASCC2 and ASCC3 (PubMed:29144457, PubMed:22055184).
Interacts directly with ASCC3, and is thereby recruited to the ASCC complex (PubMed:22055184, PubMed:29144457).
Interacts with OTUD4; the interaction is direct (PubMed:25944111).
Interacts with USP7 and USP9X (PubMed:25944111).
3 PublicationsBinary interactionsi
Hide detailsQ96Q83
With | #Exp. | IntAct |
---|---|---|
AK8 [Q96MA6] | 3 | EBI-6658697,EBI-8466265 |
GLRX3 [O76003] | 3 | EBI-6658697,EBI-374781 |
GOLGA2 [Q08379] | 5 | EBI-6658697,EBI-618309 |
IKZF1 [Q13422] | 5 | EBI-6658697,EBI-745305 |
LNX1 [Q8TBB1] | 3 | EBI-6658697,EBI-739832 |
Isoform 2 [Q96Q83-2]
Protein-protein interaction databases
BioGRIDi | 128686, 53 interactors |
IntActi | Q96Q83, 21 interactors |
STRINGi | 9606.ENSP00000302232 |
Chemistry databases
BindingDBi | Q96Q83 |
Miscellaneous databases
RNActi | Q96Q83, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q96Q83 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96Q83 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 172 – 278 | Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST | 107 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 141 – 143 | Substrate bindingBy similarity | 3 | |
Regioni | 179 – 181 | Alpha-ketoglutarate bindingCombined sources1 Publication | 3 | |
Regioni | 269 – 275 | Alpha-ketoglutarate bindingCombined sources1 Publication | 7 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QW9E, Eukaryota |
GeneTreei | ENSGT00940000157226 |
HOGENOMi | CLU_048788_2_1_1 |
InParanoidi | Q96Q83 |
OMAi | TMQPNPN |
OrthoDBi | 1494495at2759 |
PhylomeDBi | Q96Q83 |
TreeFami | TF331732 |
Family and domain databases
Gene3Di | 2.60.120.590, 1 hit |
InterProi | View protein in InterPro IPR027450, AlkB-like IPR037151, AlkB-like_sf IPR032854, ALKBH3 IPR005123, Oxoglu/Fe-dep_dioxygenase |
PANTHERi | PTHR31212, PTHR31212, 1 hit |
Pfami | View protein in Pfam PF13532, 2OG-FeII_Oxy_2, 1 hit |
PROSITEi | View protein in PROSITE PS51471, FE2OG_OXY, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEEKRRRARV QGAWAAPVKS QAIAQPATTA KSHLHQKPGQ TWKNKEHHLS
60 70 80 90 100
DREFVFKEPQ QVVRRAPEPR VIDREGVYEI SLSPTGVSRV CLYPGFVDVK
110 120 130 140 150
EADWILEQLC QDVPWKQRTG IREDITYQQP RLTAWYGELP YTYSRITMEP
160 170 180 190 200
NPHWHPVLRT LKNRIEENTG HTFNSLLCNL YRNEKDSVDW HSDDEPSLGR
210 220 230 240 250
CPIIASLSFG ATRTFEMRKK PPPEENGDYT YVERVKIPLD HGTLLIMEGA
260 270 280
TQADWQHRVP KEYHSREPRV NLTFRTVYPD PRGAPW
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PSA5 | E9PSA5_HUMAN | Alpha-ketoglutarate-dependent dioxy... | ALKBH3 | 223 | Annotation score: | ||
H0YDH4 | H0YDH4_HUMAN | Alpha-ketoglutarate-dependent dioxy... | ALKBH3 | 156 | Annotation score: | ||
E9PN35 | E9PN35_HUMAN | Alpha-ketoglutarate-dependent dioxy... | ALKBH3 | 171 | Annotation score: | ||
E9PRJ2 | E9PRJ2_HUMAN | Alpha-ketoglutarate-dependent dioxy... | ALKBH3 | 66 | Annotation score: | ||
E9PRD0 | E9PRD0_HUMAN | Alpha-ketoglutarate-dependent dioxy... | ALKBH3 | 60 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_026632 | 164 | R → C1 PublicationCorresponds to variant dbSNP:rs2271815Ensembl. | 1 | |
Natural variantiVAR_026631 | 228 | D → E2 PublicationsCorresponds to variant dbSNP:rs1130290Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019125 | 73 – 74 | DR → E in isoform 2. 1 Publication | 2 | |
Alternative sequenceiVSP_019126 | 125 – 172 | ITYQQ…NTGHT → SILQLTFKKSAPVSGTATAP QSCWYERPSPPHIPGPAILT RTRLWAP in isoform 2. 1 PublicationAdd BLAST | 48 | |
Alternative sequenceiVSP_019127 | 173 – 286 | Missing in isoform 2. 1 PublicationAdd BLAST | 114 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB042029 mRNA Translation: BAB70508.1 DQ196343 Genomic DNA Translation: ABA27096.1 AC087521 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW68078.1 BC015155 mRNA Translation: AAH15155.1 Different initiation. BC103812 mRNA Translation: AAI03813.1 BC103813 mRNA Translation: AAI03814.1 BC103814 mRNA Translation: AAI03815.1 BC067257 mRNA Translation: AAH67257.1 |
CCDSi | CCDS7906.1 [Q96Q83-1] |
RefSeqi | NP_631917.1, NM_139178.3 [Q96Q83-1] |
Genome annotation databases
Ensembli | ENST00000302708; ENSP00000302232; ENSG00000166199 [Q96Q83-1] ENST00000530803; ENSP00000436788; ENSG00000166199 [Q96Q83-2] |
GeneIDi | 221120 |
KEGGi | hsa:221120 |
UCSCi | uc001mxs.3, human [Q96Q83-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB042029 mRNA Translation: BAB70508.1 DQ196343 Genomic DNA Translation: ABA27096.1 AC087521 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW68078.1 BC015155 mRNA Translation: AAH15155.1 Different initiation. BC103812 mRNA Translation: AAI03813.1 BC103813 mRNA Translation: AAI03814.1 BC103814 mRNA Translation: AAI03815.1 BC067257 mRNA Translation: AAH67257.1 |
CCDSi | CCDS7906.1 [Q96Q83-1] |
RefSeqi | NP_631917.1, NM_139178.3 [Q96Q83-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2IUW | X-ray | 1.50 | A | 70-286 | [»] | |
SMRi | Q96Q83 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 128686, 53 interactors |
IntActi | Q96Q83, 21 interactors |
STRINGi | 9606.ENSP00000302232 |
Chemistry databases
BindingDBi | Q96Q83 |
ChEMBLi | CHEMBL3112376 |
DrugBanki | DB00126, Ascorbic acid |
PTM databases
iPTMneti | Q96Q83 |
PhosphoSitePlusi | Q96Q83 |
Genetic variation databases
BioMutai | ALKBH3 |
DMDMi | 74752087 |
Proteomic databases
EPDi | Q96Q83 |
jPOSTi | Q96Q83 |
MassIVEi | Q96Q83 |
MaxQBi | Q96Q83 |
PaxDbi | Q96Q83 |
PeptideAtlasi | Q96Q83 |
PRIDEi | Q96Q83 |
ProteomicsDBi | 77836 [Q96Q83-1] 77837 [Q96Q83-2] |
Protocols and materials databases
Antibodypediai | 1876, 253 antibodies |
Genome annotation databases
Ensembli | ENST00000302708; ENSP00000302232; ENSG00000166199 [Q96Q83-1] ENST00000530803; ENSP00000436788; ENSG00000166199 [Q96Q83-2] |
GeneIDi | 221120 |
KEGGi | hsa:221120 |
UCSCi | uc001mxs.3, human [Q96Q83-1] |
Organism-specific databases
CTDi | 221120 |
DisGeNETi | 221120 |
GeneCardsi | ALKBH3 |
HGNCi | HGNC:30141, ALKBH3 |
HPAi | ENSG00000166199, Low tissue specificity |
MIMi | 610603, gene |
neXtProti | NX_Q96Q83 |
OpenTargetsi | ENSG00000166199 |
PharmGKBi | PA143485293 |
VEuPathDBi | HostDB:ENSG00000166199.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QW9E, Eukaryota |
GeneTreei | ENSGT00940000157226 |
HOGENOMi | CLU_048788_2_1_1 |
InParanoidi | Q96Q83 |
OMAi | TMQPNPN |
OrthoDBi | 1494495at2759 |
PhylomeDBi | Q96Q83 |
TreeFami | TF331732 |
Enzyme and pathway databases
BRENDAi | 1.14.11.33, 2681 |
PathwayCommonsi | Q96Q83 |
Reactomei | R-HSA-112126, ALKBH3 mediated reversal of alkylation damage |
Miscellaneous databases
BioGRID-ORCSi | 221120, 14 hits in 999 CRISPR screens |
ChiTaRSi | ALKBH3, human |
EvolutionaryTracei | Q96Q83 |
GenomeRNAii | 221120 |
Pharosi | Q96Q83, Tchem |
PROi | PR:Q96Q83 |
RNActi | Q96Q83, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000166199, Expressed in skeletal muscle tissue and 187 other tissues |
ExpressionAtlasi | Q96Q83, baseline and differential |
Genevisiblei | Q96Q83, HS |
Family and domain databases
Gene3Di | 2.60.120.590, 1 hit |
InterProi | View protein in InterPro IPR027450, AlkB-like IPR037151, AlkB-like_sf IPR032854, ALKBH3 IPR005123, Oxoglu/Fe-dep_dioxygenase |
PANTHERi | PTHR31212, PTHR31212, 1 hit |
Pfami | View protein in Pfam PF13532, 2OG-FeII_Oxy_2, 1 hit |
PROSITEi | View protein in PROSITE PS51471, FE2OG_OXY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALKB3_HUMAN | |
Accessioni | Q96Q83Primary (citable) accession number: Q96Q83 Secondary accession number(s): A6NDJ1 Q96BU8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2006 |
Last sequence update: | December 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families