UniProtKB - Q96PM5 (ZN363_HUMAN)
RING finger and CHY zinc finger domain-containing protein 1
RCHY1
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 13 – 80 | CHY-typePROSITE-ProRule annotationAdd BLAST | 68 | |
Zinc fingeri | 82 – 144 | CTCHY-typePROSITE-ProRule annotationAdd BLAST | 63 | |
Zinc fingeri | 145 – 189 | RING-typePROSITE-ProRule annotationAdd BLAST | 45 |
GO - Molecular functioni
- p53 binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- ubiquitin protein ligase activity Source: UniProtKB
- ubiquitin-protein transferase activity Source: Reactome
- zinc ion binding Source: UniProtKB
GO - Biological processi
- error-free translesion synthesis Source: Reactome
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- positive regulation of protein ubiquitination Source: UniProtKB
- protein autoubiquitination Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 2.3.2.B10, 2681 6.3.2.19, 2681 |
PathwayCommonsi | Q96PM5 |
Reactomei | R-HSA-110320, Translesion Synthesis by POLH R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SIGNORi | Q96PM5 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: RING finger and CHY zinc finger domain-containing protein 1 (EC:2.3.2.27)Alternative name(s): Androgen receptor N-terminal-interacting protein CH-rich-interacting match with PLAG1 E3 ubiquitin-protein ligase Pirh2 RING finger protein 199 RING-type E3 ubiquitin transferase RCHY1 Zinc finger protein 363 p53-induced RING-H2 protein Short name: hPirh2 |
Gene namesi | Name:RCHY1 Synonyms:ARNIP, CHIMP, PIRH2, RNF199, ZNF363 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000163743.13 |
HGNCi | HGNC:17479, RCHY1 |
MIMi | 607680, gene |
neXtProti | NX_Q96PM5 |
Subcellular locationi
Nucleus
Other locations
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: HGNC-UCL
- intracellular membrane-bounded organelle Source: HPA
- ubiquitin ligase complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 176 | M → E: Abolishes E3 ubiquitin-protein ligase activity. 1 Publication | 1 | |
Mutagenesisi | 186 | C → A: Abolishes E3 ubiquitin-protein ligase activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 25898 |
OpenTargetsi | ENSG00000163743 |
PharmGKBi | PA38240 |
Miscellaneous databases
Pharosi | Q96PM5, Tbio |
Polymorphism and mutation databases
BioMutai | RCHY1 |
DMDMi | 32700008 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056312 | 1 – 261 | RING finger and CHY zinc finger domain-containing protein 1Add BLAST | 261 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 257 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q96PM5 |
jPOSTi | Q96PM5 |
MassIVEi | Q96PM5 |
MaxQBi | Q96PM5 |
PaxDbi | Q96PM5 |
PeptideAtlasi | Q96PM5 |
PRIDEi | Q96PM5 |
ProteomicsDBi | 16915 18304 61327 77709 [Q96PM5-1] 77710 [Q96PM5-2] 77711 [Q96PM5-3] |
2D gel databases
REPRODUCTION-2DPAGEi | Q96PM5 |
PTM databases
iPTMneti | Q96PM5 |
PhosphoSitePlusi | Q96PM5 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSG00000163743, Expressed in sperm and 250 other tissues |
ExpressionAtlasi | Q96PM5, baseline and differential |
Genevisiblei | Q96PM5, HS |
Organism-specific databases
HPAi | ENSG00000163743, Low tissue specificity |
Interactioni
Subunit structurei
Monomer and homodimer.
Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, CDKN1B, COPE, UBE2D2 and GORAB/NTKLBP1.
8 PublicationsBinary interactionsi
Hide detailsQ96PM5
GO - Molecular functioni
- p53 binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 117406, 96 interactors |
DIPi | DIP-43981N |
IntActi | Q96PM5, 53 interactors |
MINTi | Q96PM5 |
STRINGi | 9606.ENSP00000321239 |
Miscellaneous databases
RNActi | Q96PM5, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q96PM5 |
SMRi | Q96PM5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96PM5 |
Family & Domainsi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 13 – 80 | CHY-typePROSITE-ProRule annotationAdd BLAST | 68 | |
Zinc fingeri | 82 – 144 | CTCHY-typePROSITE-ProRule annotationAdd BLAST | 63 | |
Zinc fingeri | 145 – 189 | RING-typePROSITE-ProRule annotationAdd BLAST | 45 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1940, Eukaryota |
GeneTreei | ENSGT00390000008853 |
HOGENOMi | CLU_013368_3_0_1 |
InParanoidi | Q96PM5 |
OMAi | TCFDDMV |
OrthoDBi | 1303946at2759 |
PhylomeDBi | Q96PM5 |
TreeFami | TF323762 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR039512, RCHY1_zinc-ribbon IPR008913, Znf_CHY IPR037274, Znf_CHY_sf IPR017921, Znf_CTCHY IPR037275, Znf_CTCHY_sf IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF05495, zf-CHY, 1 hit PF13639, zf-RING_2, 1 hit PF14599, zinc_ribbon_6, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
SUPFAMi | SSF161219, SSF161219, 1 hit SSF161245, SSF161245, 1 hit |
PROSITEi | View protein in PROSITE PS51266, ZF_CHY, 1 hit PS51270, ZF_CTCHY, 1 hit PS50089, ZF_RING_2, 1 hit |
s (8+)i Sequence
Sequence statusi: Complete.
This entry describes 8 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 8 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAATAREDGA SGQERGQRGC EHYDRGCLLK APCCDKLYTC RLCHDNNEDH
60 70 80 90 100
QLDRFKVKEV QCINCEKIQH AQQTCEECST LFGEYYCDIC HLFDKDKKQY
110 120 130 140 150
HCENCGICRI GPKEDFFHCL KCNLCLAMNL QGRHKCIENV SRQNCPICLE
160 170 180 190 200
DIHTSRVVAH VLPCGHLLHR TCYEEMLKEG YRCPLCMHSA LDMTRYWRQL
210 220 230 240 250
DDEVAQTPMP SEYQNMTVDI LCNDCNGRST VQFHILGMKC KICESYNTAQ
260
AGGRRISLDQ Q
The sequence of this isoform differs from the canonical sequence as follows:
180-261: GYRCPLCMHS...GGRRISLDQQ → YDQVLETAG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YAI4 | H0YAI4_HUMAN | RING finger and CHY zinc finger dom... | RCHY1 | 111 | Annotation score: | ||
D6RAF6 | D6RAF6_HUMAN | RING finger and CHY zinc finger dom... | RCHY1 | 107 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 11 – 13 | SGQ → TGE in ACT35531 (PubMed:19483087).Curated | 3 | |
Sequence conflicti | 11 – 13 | SGQ → TGE in ACT35532 (PubMed:19483087).Curated | 3 | |
Sequence conflicti | 11 – 13 | SGQ → TGE in ACT35533 (PubMed:19483087).Curated | 3 | |
Sequence conflicti | 11 – 13 | SGQ → TGE in AAK96896 (Ref. 3) Curated | 3 | |
Sequence conflicti | 142 | R → Q in AAH47393 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 220 | I → F in BAD92309 (Ref. 8) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053787 | 1 – 30 | MAATA…GCLLK → MAPAVKSE in isoform 6. CuratedAdd BLAST | 30 | |
Alternative sequenceiVSP_053788 | 31 – 70 | Missing in isoform 7 and isoform 8. CuratedAdd BLAST | 40 | |
Alternative sequenceiVSP_053385 | 68 – 75 | IQHAQQTC → NSTCPTDL in isoform 5. 1 Publication | 8 | |
Alternative sequenceiVSP_053386 | 76 – 261 | Missing in isoform 5. 1 PublicationAdd BLAST | 186 | |
Alternative sequenceiVSP_038467 | 171 – 179 | Missing in isoform 2 and isoform 8. 1 Publication | 9 | |
Alternative sequenceiVSP_038468 | 180 – 261 | Missing in isoform 3. 1 PublicationAdd BLAST | 82 | |
Alternative sequenceiVSP_044085 | 180 – 261 | GYRCP…SLDQQ → YDQVLETAG in isoform 4. 1 PublicationAdd BLAST | 82 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GQ250944 mRNA Translation: ACT35531.1 GQ250945 mRNA Translation: ACT35532.1 GQ250946 mRNA Translation: ACT35533.1 AF247041 mRNA Translation: AAL76101.1 AF255666 mRNA Translation: AAK96896.1 AF305424 mRNA Translation: AAL09356.1 AB209072 mRNA Translation: BAD92309.1 Different initiation. AY888047 mRNA Translation: AAX78233.1 GU937000 mRNA Translation: ADD21555.1 AK091501 mRNA Translation: BAG52375.1 AC096759 Genomic DNA No translation available. CH471057 Genomic DNA Translation: EAX05725.1 BC047393 mRNA Translation: AAH47393.1 |
CCDSi | CCDS34012.1 [Q96PM5-2] CCDS3567.1 [Q96PM5-1] CCDS63990.1 [Q96PM5-8] CCDS63991.1 [Q96PM5-7] CCDS63992.1 [Q96PM5-6] |
RefSeqi | NP_001009922.1, NM_001009922.2 [Q96PM5-2] NP_001265465.1, NM_001278536.1 [Q96PM5-7] NP_001265466.1, NM_001278537.1 [Q96PM5-8] NP_001265467.1, NM_001278538.1 [Q96PM5-6] NP_056251.2, NM_015436.3 [Q96PM5-1] |
Genome annotation databases
Ensembli | ENST00000324439; ENSP00000321239; ENSG00000163743 [Q96PM5-1] ENST00000380840; ENSP00000370220; ENSG00000163743 [Q96PM5-7] ENST00000505105; ENSP00000424631; ENSG00000163743 [Q96PM5-4] ENST00000507014; ENSP00000424472; ENSG00000163743 [Q96PM5-8] ENST00000512706; ENSP00000423976; ENSG00000163743 [Q96PM5-6] ENST00000513257; ENSP00000421084; ENSG00000163743 [Q96PM5-2] |
GeneIDi | 25898 |
KEGGi | hsa:25898 |
UCSCi | uc003hik.4, human [Q96PM5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GQ250944 mRNA Translation: ACT35531.1 GQ250945 mRNA Translation: ACT35532.1 GQ250946 mRNA Translation: ACT35533.1 AF247041 mRNA Translation: AAL76101.1 AF255666 mRNA Translation: AAK96896.1 AF305424 mRNA Translation: AAL09356.1 AB209072 mRNA Translation: BAD92309.1 Different initiation. AY888047 mRNA Translation: AAX78233.1 GU937000 mRNA Translation: ADD21555.1 AK091501 mRNA Translation: BAG52375.1 AC096759 Genomic DNA No translation available. CH471057 Genomic DNA Translation: EAX05725.1 BC047393 mRNA Translation: AAH47393.1 |
CCDSi | CCDS34012.1 [Q96PM5-2] CCDS3567.1 [Q96PM5-1] CCDS63990.1 [Q96PM5-8] CCDS63991.1 [Q96PM5-7] CCDS63992.1 [Q96PM5-6] |
RefSeqi | NP_001009922.1, NM_001009922.2 [Q96PM5-2] NP_001265465.1, NM_001278536.1 [Q96PM5-7] NP_001265466.1, NM_001278537.1 [Q96PM5-8] NP_001265467.1, NM_001278538.1 [Q96PM5-6] NP_056251.2, NM_015436.3 [Q96PM5-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JRJ | NMR | - | A | 138-189 | [»] | |
2K2C | NMR | - | A | 1-137 | [»] | |
2K2D | NMR | - | A | 187-261 | [»] | |
BMRBi | Q96PM5 | |||||
SMRi | Q96PM5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117406, 96 interactors |
DIPi | DIP-43981N |
IntActi | Q96PM5, 53 interactors |
MINTi | Q96PM5 |
STRINGi | 9606.ENSP00000321239 |
PTM databases
iPTMneti | Q96PM5 |
PhosphoSitePlusi | Q96PM5 |
Polymorphism and mutation databases
BioMutai | RCHY1 |
DMDMi | 32700008 |
2D gel databases
REPRODUCTION-2DPAGEi | Q96PM5 |
Proteomic databases
EPDi | Q96PM5 |
jPOSTi | Q96PM5 |
MassIVEi | Q96PM5 |
MaxQBi | Q96PM5 |
PaxDbi | Q96PM5 |
PeptideAtlasi | Q96PM5 |
PRIDEi | Q96PM5 |
ProteomicsDBi | 16915 18304 61327 77709 [Q96PM5-1] 77710 [Q96PM5-2] 77711 [Q96PM5-3] |
Protocols and materials databases
Antibodypediai | 24650, 184 antibodies |
DNASUi | 25898 |
Genome annotation databases
Ensembli | ENST00000324439; ENSP00000321239; ENSG00000163743 [Q96PM5-1] ENST00000380840; ENSP00000370220; ENSG00000163743 [Q96PM5-7] ENST00000505105; ENSP00000424631; ENSG00000163743 [Q96PM5-4] ENST00000507014; ENSP00000424472; ENSG00000163743 [Q96PM5-8] ENST00000512706; ENSP00000423976; ENSG00000163743 [Q96PM5-6] ENST00000513257; ENSP00000421084; ENSG00000163743 [Q96PM5-2] |
GeneIDi | 25898 |
KEGGi | hsa:25898 |
UCSCi | uc003hik.4, human [Q96PM5-1] |
Organism-specific databases
CTDi | 25898 |
DisGeNETi | 25898 |
EuPathDBi | HostDB:ENSG00000163743.13 |
GeneCardsi | RCHY1 |
HGNCi | HGNC:17479, RCHY1 |
HPAi | ENSG00000163743, Low tissue specificity |
MIMi | 607680, gene |
neXtProti | NX_Q96PM5 |
OpenTargetsi | ENSG00000163743 |
PharmGKBi | PA38240 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1940, Eukaryota |
GeneTreei | ENSGT00390000008853 |
HOGENOMi | CLU_013368_3_0_1 |
InParanoidi | Q96PM5 |
OMAi | TCFDDMV |
OrthoDBi | 1303946at2759 |
PhylomeDBi | Q96PM5 |
TreeFami | TF323762 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
BRENDAi | 2.3.2.B10, 2681 6.3.2.19, 2681 |
PathwayCommonsi | Q96PM5 |
Reactomei | R-HSA-110320, Translesion Synthesis by POLH R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SIGNORi | Q96PM5 |
Miscellaneous databases
BioGRID-ORCSi | 25898, 6 hits in 850 CRISPR screens |
ChiTaRSi | RCHY1, human |
EvolutionaryTracei | Q96PM5 |
GeneWikii | RCHY1 |
GenomeRNAii | 25898 |
Pharosi | Q96PM5, Tbio |
PROi | PR:Q96PM5 |
RNActi | Q96PM5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163743, Expressed in sperm and 250 other tissues |
ExpressionAtlasi | Q96PM5, baseline and differential |
Genevisiblei | Q96PM5, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR039512, RCHY1_zinc-ribbon IPR008913, Znf_CHY IPR037274, Znf_CHY_sf IPR017921, Znf_CTCHY IPR037275, Znf_CTCHY_sf IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF05495, zf-CHY, 1 hit PF13639, zf-RING_2, 1 hit PF14599, zinc_ribbon_6, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
SUPFAMi | SSF161219, SSF161219, 1 hit SSF161245, SSF161245, 1 hit |
PROSITEi | View protein in PROSITE PS51266, ZF_CHY, 1 hit PS51270, ZF_CTCHY, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZN363_HUMAN | |
Accessioni | Q96PM5Primary (citable) accession number: Q96PM5 Secondary accession number(s): B3KRG3 Q96PR5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 28, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references