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Protein

RING finger and CHY zinc finger domain-containing protein 1

Gene

RCHY1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.7 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri13 – 80CHY-typePROSITE-ProRule annotationAdd BLAST68
Zinc fingeri82 – 144CTCHY-typePROSITE-ProRule annotationAdd BLAST63
Zinc fingeri145 – 189RING-typePROSITE-ProRule annotationAdd BLAST45

GO - Molecular functioni

  • p53 binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • signaling receptor binding Source: Ensembl
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.2.B10 2681
6.3.2.19 2681
ReactomeiR-HSA-110320 Translesion Synthesis by POLH
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SIGNORiQ96PM5
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
RING finger and CHY zinc finger domain-containing protein 1 (EC:2.3.2.27)
Alternative name(s):
Androgen receptor N-terminal-interacting protein
CH-rich-interacting match with PLAG1
E3 ubiquitin-protein ligase Pirh2
RING finger protein 199
RING-type E3 ubiquitin transferase RCHY1
Zinc finger protein 363
p53-induced RING-H2 protein
Short name:
hPirh2
Gene namesi
Name:RCHY1
Synonyms:ARNIP, CHIMP, PIRH2, RNF199, ZNF363
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000163743.13
HGNCiHGNC:17479 RCHY1
MIMi607680 gene
neXtProtiNX_Q96PM5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176M → E: Abolishes E3 ubiquitin-protein ligase activity. 1 Publication1
Mutagenesisi186C → A: Abolishes E3 ubiquitin-protein ligase activity. 1 Publication1

Organism-specific databases

DisGeNETi25898
OpenTargetsiENSG00000163743
PharmGKBiPA38240

Polymorphism and mutation databases

BioMutaiRCHY1
DMDMi32700008

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000563121 – 261RING finger and CHY zinc finger domain-containing protein 1Add BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei257PhosphoserineCombined sources1

Post-translational modificationi

Subject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability.2 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96PM5
MaxQBiQ96PM5
PaxDbiQ96PM5
PeptideAtlasiQ96PM5
PRIDEiQ96PM5
ProteomicsDBi77709
77710 [Q96PM5-2]
77711 [Q96PM5-3]

2D gel databases

REPRODUCTION-2DPAGEiQ96PM5

PTM databases

iPTMnetiQ96PM5
PhosphoSitePlusiQ96PM5

Expressioni

Inductioni

Up-regulated during the S phase of the cell cycle. Expressed at low levels during G phase.

Gene expression databases

BgeeiENSG00000163743 Expressed in 236 organ(s), highest expression level in sperm
CleanExiHS_RCHY1
ExpressionAtlasiQ96PM5 baseline and differential
GenevisibleiQ96PM5 HS

Organism-specific databases

HPAiHPA030339

Interactioni

Subunit structurei

Monomer and homodimer. Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, CDKN1B, COPE, UBE2D2 and GORAB/NTKLBP1.8 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117406, 84 interactors
DIPiDIP-43981N
IntActiQ96PM5, 51 interactors
MINTiQ96PM5
STRINGi9606.ENSP00000321239

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96PM5
SMRiQ96PM5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96PM5

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri13 – 80CHY-typePROSITE-ProRule annotationAdd BLAST68
Zinc fingeri82 – 144CTCHY-typePROSITE-ProRule annotationAdd BLAST63
Zinc fingeri145 – 189RING-typePROSITE-ProRule annotationAdd BLAST45

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1940 Eukaryota
ENOG410XXHF LUCA
GeneTreeiENSGT00390000008853
HOGENOMiHOG000231827
HOVERGENiHBG062959
InParanoidiQ96PM5
KOiK10144
OMAiGMKCNIC
OrthoDBiEOG091G094U
PhylomeDBiQ96PM5
TreeFamiTF323762

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR039512 RCHY1_zinc-ribbon
IPR008913 Znf_CHY
IPR037274 Znf_CHY_sf
IPR017921 Znf_CTCHY
IPR037275 Znf_CTCHY_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF05495 zf-CHY, 1 hit
PF13639 zf-RING_2, 1 hit
PF14599 zinc_ribbon_6, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SUPFAMiSSF161219 SSF161219, 1 hit
SSF161245 SSF161245, 1 hit
PROSITEiView protein in PROSITE
PS51266 ZF_CHY, 1 hit
PS51270 ZF_CTCHY, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (8+)i

Sequence statusi: Complete.

This entry describes 8 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 8 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96PM5-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAATAREDGA SGQERGQRGC EHYDRGCLLK APCCDKLYTC RLCHDNNEDH
60 70 80 90 100
QLDRFKVKEV QCINCEKIQH AQQTCEECST LFGEYYCDIC HLFDKDKKQY
110 120 130 140 150
HCENCGICRI GPKEDFFHCL KCNLCLAMNL QGRHKCIENV SRQNCPICLE
160 170 180 190 200
DIHTSRVVAH VLPCGHLLHR TCYEEMLKEG YRCPLCMHSA LDMTRYWRQL
210 220 230 240 250
DDEVAQTPMP SEYQNMTVDI LCNDCNGRST VQFHILGMKC KICESYNTAQ
260
AGGRRISLDQ Q
Length:261
Mass (Da):30,110
Last modified:December 1, 2001 - v1
Checksum:iAC03786F6B42A03D
GO
Isoform 2 (identifier: Q96PM5-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     171-179: Missing.

Show »
Length:252
Mass (Da):28,983
Checksum:i0079B97723751278
GO
Isoform 3 (identifier: Q96PM5-3) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     180-261: Missing.

Show »
Length:179
Mass (Da):20,720
Checksum:iB38F75D4374FA2F8
GO
Isoform 4 (identifier: Q96PM5-4) [UniParc]FASTAAdd to basket
Also known as: Pirh2b

The sequence of this isoform differs from the canonical sequence as follows:
     180-261: GYRCPLCMHS...GGRRISLDQQ → YDQVLETAG

Note: No ubiquitin protein ligase activity. Down-regulated in hepatocellular carcinoma.
Show »
Length:188
Mass (Da):21,697
Checksum:i0DEFC07DCEDBA3A7
GO
Isoform 5 (identifier: Q96PM5-5) [UniParc]FASTAAdd to basket
Also known as: Pirh2D

The sequence of this isoform differs from the canonical sequence as follows:
     68-75: IQHAQQTC → NSTCPTDL
     76-261: Missing.

Show »
Length:75
Mass (Da):8,494
Checksum:i2FE97DE89608DE6D
GO
Isoform 6 (identifier: Q96PM5-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: MAATAREDGASGQERGQRGCEHYDRGCLLK → MAPAVKSE

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:239
Mass (Da):27,676
Checksum:i0DD872A14E2E217A
GO
Isoform 7 (identifier: Q96PM5-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     31-70: Missing.

Note: No experimental confirmation available. Gene prediction based on partial mRNA data.
Show »
Length:221
Mass (Da):25,337
Checksum:i7B96797039B25C4D
GO
Isoform 8 (identifier: Q96PM5-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     31-70: Missing.
     171-179: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:212
Mass (Da):24,210
Checksum:iA4EA203D3FBB7F21
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RAF6D6RAF6_HUMAN
RING finger and CHY zinc finger dom...
RCHY1
107Annotation score:
H0YAI4H0YAI4_HUMAN
RING finger and CHY zinc finger dom...
RCHY1
111Annotation score:

Sequence cautioni

The sequence BAD92309 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11 – 13SGQ → TGE in ACT35531 (PubMed:19483087).Curated3
Sequence conflicti11 – 13SGQ → TGE in ACT35532 (PubMed:19483087).Curated3
Sequence conflicti11 – 13SGQ → TGE in ACT35533 (PubMed:19483087).Curated3
Sequence conflicti11 – 13SGQ → TGE in AAK96896 (Ref. 3) Curated3
Sequence conflicti142R → Q in AAH47393 (PubMed:15489334).Curated1
Sequence conflicti220I → F in BAD92309 (Ref. 8) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0537871 – 30MAATA…GCLLK → MAPAVKSE in isoform 6. CuratedAdd BLAST30
Alternative sequenceiVSP_05378831 – 70Missing in isoform 7 and isoform 8. CuratedAdd BLAST40
Alternative sequenceiVSP_05338568 – 75IQHAQQTC → NSTCPTDL in isoform 5. 1 Publication8
Alternative sequenceiVSP_05338676 – 261Missing in isoform 5. 1 PublicationAdd BLAST186
Alternative sequenceiVSP_038467171 – 179Missing in isoform 2 and isoform 8. 1 Publication9
Alternative sequenceiVSP_038468180 – 261Missing in isoform 3. 1 PublicationAdd BLAST82
Alternative sequenceiVSP_044085180 – 261GYRCP…SLDQQ → YDQVLETAG in isoform 4. 1 PublicationAdd BLAST82

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ250944 mRNA Translation: ACT35531.1
GQ250945 mRNA Translation: ACT35532.1
GQ250946 mRNA Translation: ACT35533.1
AF247041 mRNA Translation: AAL76101.1
AF255666 mRNA Translation: AAK96896.1
AF305424 mRNA Translation: AAL09356.1
AB209072 mRNA Translation: BAD92309.1 Different initiation.
AY888047 mRNA Translation: AAX78233.1
GU937000 mRNA Translation: ADD21555.1
AK091501 mRNA Translation: BAG52375.1
AC096759 Genomic DNA No translation available.
CH471057 Genomic DNA Translation: EAX05725.1
BC047393 mRNA Translation: AAH47393.1
CCDSiCCDS34012.1 [Q96PM5-2]
CCDS3567.1 [Q96PM5-1]
CCDS63990.1 [Q96PM5-8]
CCDS63991.1 [Q96PM5-7]
CCDS63992.1 [Q96PM5-6]
RefSeqiNP_001009922.1, NM_001009922.2 [Q96PM5-2]
NP_001265465.1, NM_001278536.1 [Q96PM5-7]
NP_001265466.1, NM_001278537.1 [Q96PM5-8]
NP_001265467.1, NM_001278538.1 [Q96PM5-6]
NP_056251.2, NM_015436.3 [Q96PM5-1]
UniGeneiHs.48297

Genome annotation databases

EnsembliENST00000324439; ENSP00000321239; ENSG00000163743 [Q96PM5-1]
ENST00000380840; ENSP00000370220; ENSG00000163743 [Q96PM5-7]
ENST00000505105; ENSP00000424631; ENSG00000163743 [Q96PM5-4]
ENST00000507014; ENSP00000424472; ENSG00000163743 [Q96PM5-8]
ENST00000512706; ENSP00000423976; ENSG00000163743 [Q96PM5-6]
ENST00000513257; ENSP00000421084; ENSG00000163743 [Q96PM5-2]
GeneIDi25898
KEGGihsa:25898
UCSCiuc003hik.4 human [Q96PM5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ250944 mRNA Translation: ACT35531.1
GQ250945 mRNA Translation: ACT35532.1
GQ250946 mRNA Translation: ACT35533.1
AF247041 mRNA Translation: AAL76101.1
AF255666 mRNA Translation: AAK96896.1
AF305424 mRNA Translation: AAL09356.1
AB209072 mRNA Translation: BAD92309.1 Different initiation.
AY888047 mRNA Translation: AAX78233.1
GU937000 mRNA Translation: ADD21555.1
AK091501 mRNA Translation: BAG52375.1
AC096759 Genomic DNA No translation available.
CH471057 Genomic DNA Translation: EAX05725.1
BC047393 mRNA Translation: AAH47393.1
CCDSiCCDS34012.1 [Q96PM5-2]
CCDS3567.1 [Q96PM5-1]
CCDS63990.1 [Q96PM5-8]
CCDS63991.1 [Q96PM5-7]
CCDS63992.1 [Q96PM5-6]
RefSeqiNP_001009922.1, NM_001009922.2 [Q96PM5-2]
NP_001265465.1, NM_001278536.1 [Q96PM5-7]
NP_001265466.1, NM_001278537.1 [Q96PM5-8]
NP_001265467.1, NM_001278538.1 [Q96PM5-6]
NP_056251.2, NM_015436.3 [Q96PM5-1]
UniGeneiHs.48297

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JRJNMR-A138-189[»]
2K2CNMR-A1-137[»]
2K2DNMR-A187-261[»]
ProteinModelPortaliQ96PM5
SMRiQ96PM5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117406, 84 interactors
DIPiDIP-43981N
IntActiQ96PM5, 51 interactors
MINTiQ96PM5
STRINGi9606.ENSP00000321239

PTM databases

iPTMnetiQ96PM5
PhosphoSitePlusiQ96PM5

Polymorphism and mutation databases

BioMutaiRCHY1
DMDMi32700008

2D gel databases

REPRODUCTION-2DPAGEiQ96PM5

Proteomic databases

EPDiQ96PM5
MaxQBiQ96PM5
PaxDbiQ96PM5
PeptideAtlasiQ96PM5
PRIDEiQ96PM5
ProteomicsDBi77709
77710 [Q96PM5-2]
77711 [Q96PM5-3]

Protocols and materials databases

DNASUi25898
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324439; ENSP00000321239; ENSG00000163743 [Q96PM5-1]
ENST00000380840; ENSP00000370220; ENSG00000163743 [Q96PM5-7]
ENST00000505105; ENSP00000424631; ENSG00000163743 [Q96PM5-4]
ENST00000507014; ENSP00000424472; ENSG00000163743 [Q96PM5-8]
ENST00000512706; ENSP00000423976; ENSG00000163743 [Q96PM5-6]
ENST00000513257; ENSP00000421084; ENSG00000163743 [Q96PM5-2]
GeneIDi25898
KEGGihsa:25898
UCSCiuc003hik.4 human [Q96PM5-1]

Organism-specific databases

CTDi25898
DisGeNETi25898
EuPathDBiHostDB:ENSG00000163743.13
GeneCardsiRCHY1
HGNCiHGNC:17479 RCHY1
HPAiHPA030339
MIMi607680 gene
neXtProtiNX_Q96PM5
OpenTargetsiENSG00000163743
PharmGKBiPA38240
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1940 Eukaryota
ENOG410XXHF LUCA
GeneTreeiENSGT00390000008853
HOGENOMiHOG000231827
HOVERGENiHBG062959
InParanoidiQ96PM5
KOiK10144
OMAiGMKCNIC
OrthoDBiEOG091G094U
PhylomeDBiQ96PM5
TreeFamiTF323762

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi2.3.2.B10 2681
6.3.2.19 2681
ReactomeiR-HSA-110320 Translesion Synthesis by POLH
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SIGNORiQ96PM5

Miscellaneous databases

ChiTaRSiRCHY1 human
EvolutionaryTraceiQ96PM5
GeneWikiiRCHY1
GenomeRNAii25898
PROiPR:Q96PM5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163743 Expressed in 236 organ(s), highest expression level in sperm
CleanExiHS_RCHY1
ExpressionAtlasiQ96PM5 baseline and differential
GenevisibleiQ96PM5 HS

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR039512 RCHY1_zinc-ribbon
IPR008913 Znf_CHY
IPR037274 Znf_CHY_sf
IPR017921 Znf_CTCHY
IPR037275 Znf_CTCHY_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF05495 zf-CHY, 1 hit
PF13639 zf-RING_2, 1 hit
PF14599 zinc_ribbon_6, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SUPFAMiSSF161219 SSF161219, 1 hit
SSF161245 SSF161245, 1 hit
PROSITEiView protein in PROSITE
PS51266 ZF_CHY, 1 hit
PS51270 ZF_CTCHY, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZN363_HUMAN
AccessioniPrimary (citable) accession number: Q96PM5
Secondary accession number(s): B3KRG3
, C7E541, C7E542, C7E543, D3YRV2, E7EMC8, E7ETW5, J3KPI0, Q2KN33, Q59GN7, Q86X26, Q96PR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: December 1, 2001
Last modified: October 10, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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