UniProtKB - Q96PD7 (DGAT2_HUMAN)
Diacylglycerol O-acyltransferase 2
DGAT2
Functioni
Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides (PubMed:27184406).
Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity).
Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity).
Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).
By similarity2 PublicationsCatalytic activityi
- EC:2.3.1.202 PublicationsThis reaction proceeds in the forwardCurated direction.
- EC:2.3.1.761 PublicationThis reaction proceeds in the forwardCurated direction.
- (9Z)-octadecenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-glycerol = 1-O-(9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1,3-di-(9Z-octadecenoyl)-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 2,3-di-(9Z)-octadecenoyl-sn-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- 2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
: triacylglycerol biosynthesis Pathwayi
This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.
GO - Molecular functioni
- 2-acylglycerol O-acyltransferase activity Source: Ensembl
- diacylglycerol O-acyltransferase activity Source: UniProtKB
- protein homodimerization activity Source: BHF-UCL
- retinol O-fatty-acyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- bile acid signaling pathway Source: Ensembl
- cellular response to oleic acid Source: BHF-UCL
- cellular triglyceride homeostasis Source: BHF-UCL
- cholesterol homeostasis Source: BHF-UCL
- diacylglycerol biosynthetic process Source: UniProtKB
- diacylglycerol metabolic process Source: BHF-UCL
- fat pad development Source: BHF-UCL
- fatty acid homeostasis Source: BHF-UCL
- glycerol metabolic process Source: UniProtKB-KW
- lipid metabolic process Source: GO_Central
- lipid storage Source: BHF-UCL
- long-chain fatty-acyl-CoA metabolic process Source: BHF-UCL
- low-density lipoprotein particle clearance Source: BHF-UCL
- monoacylglycerol biosynthetic process Source: UniProtKB
- negative regulation of fatty acid oxidation Source: Ensembl
- positive regulation of gluconeogenesis Source: Ensembl
- positive regulation of triglyceride biosynthetic process Source: Ensembl
- regulation of cholesterol metabolic process Source: Ensembl
- regulation of lipoprotein metabolic process Source: Ensembl
- regulation of plasma lipoprotein particle levels Source: BHF-UCL
- triglyceride biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Glycerol metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.20, 2681 |
PathwayCommonsi | Q96PD7 |
Reactomei | R-HSA-1482883, Acyl chain remodeling of DAG and TAG R-HSA-75109, Triglyceride biosynthesis |
SABIO-RKi | Q96PD7 |
SignaLinki | Q96PD7 |
UniPathwayi | UPA00282 |
Chemistry databases
SwissLipidsi | SLP:000000304 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DGAT2Imported ORF Names:HMFN1045, UNQ738/PRO1433 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16940, DGAT2 |
MIMi | 606983, gene |
neXtProti | NX_Q96PD7 |
VEuPathDBi | HostDB:ENSG00000062282 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 1 Publication
Other locations
- Lipid droplet 1 Publication
- perinuclear region 1 Publication
Cytosol
- cytosol Source: HPA
Endoplasmic reticulum
- endoplasmic reticulum Source: BHF-UCL
- endoplasmic reticulum membrane Source: UniProtKB
- integral component of endoplasmic reticulum membrane Source: BHF-UCL
- perinuclear endoplasmic reticulum membrane Source: UniProtKB
Mitochondrion
- mitochondrion Source: Ensembl
Other locations
- integral component of membrane Source: BHF-UCL
- intracellular membrane-bounded organelle Source: HPA
- lipid droplet Source: UniProtKB
- perinuclear region of cytoplasm Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 69 | CytoplasmicSequence analysisAdd BLAST | 69 | |
Transmembranei | 70 – 88 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 89 – 92 | LumenalSequence analysis | 4 | |
Transmembranei | 93 – 112 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 113 – 388 | CytoplasmicSequence analysisAdd BLAST | 276 |
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Lipid droplet, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 84649 |
GeneReviewsi | DGAT2 |
MalaCardsi | DGAT2 |
OpenTargetsi | ENSG00000062282 |
Orphaneti | 487814, Autosomal dominant Charcot-Marie-Tooth disease type 2 due to DGAT2 mutation |
PharmGKBi | PA27304 |
Miscellaneous databases
Pharosi | Q96PD7, Tchem |
Chemistry databases
ChEMBLi | CHEMBL5853 |
DrugBanki | DB13961, Fish oil DB08949, Inositol nicotinate DB09568, Omega-3-carboxylic acids |
Genetic variation databases
BioMutai | DGAT2 |
DMDMi | 74732654 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249045 | 1 – 388 | Diacylglycerol O-acyltransferase 2Add BLAST | 388 |
Proteomic databases
EPDi | Q96PD7 |
jPOSTi | Q96PD7 |
MassIVEi | Q96PD7 |
PaxDbi | Q96PD7 |
PeptideAtlasi | Q96PD7 |
PRIDEi | Q96PD7 |
PTM databases
iPTMneti | Q96PD7 |
PhosphoSitePlusi | Q96PD7 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000062282, Expressed in upper arm skin and 178 other tissues |
ExpressionAtlasi | Q96PD7, baseline and differential |
Genevisiblei | Q96PD7, HS |
Organism-specific databases
HPAi | ENSG00000062282, Group enriched (adipose tissue, breast, liver, skin) |
Interactioni
Subunit structurei
Forms multimeric complexes consisting of several DGAT2 subunits (By similarity).
Interacts with SLC27A1 and this interaction is enhanced in the presence of ZFYVE1 (PubMed:30970241).
By similarity1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 124172, 6 interactors |
CORUMi | Q96PD7 |
IntActi | Q96PD7, 2 interactors |
MINTi | Q96PD7 |
STRINGi | 9606.ENSP00000228027 |
Chemistry databases
BindingDBi | Q96PD7 |
Miscellaneous databases
RNActi | Q96PD7, protein |
Structurei
3D structure databases
AlphaFoldDBi | Q96PD7 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 16 – 40 | DisorderedSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT01030000234582 |
HOGENOMi | CLU_023995_0_1_1 |
InParanoidi | Q96PD7 |
OMAi | STMFYVP |
OrthoDBi | 1347007at2759 |
PhylomeDBi | Q96PD7 |
TreeFami | TF314707 |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKTLIAAYSG VLRGERQAEA DRSQRSHGGP ALSREGSGRW GTGSSILSAL
60 70 80 90 100
QDLFSVTWLN RSKVEKQLQV ISVLQWVLSF LVLGVACSAI LMYIFCTDCW
110 120 130 140 150
LIAVLYFTWL VFDWNTPKKG GRRSQWVRNW AVWRYFRDYF PIQLVKTHNL
160 170 180 190 200
LTTRNYIFGY HPHGIMGLGA FCNFSTEATE VSKKFPGIRP YLATLAGNFR
210 220 230 240 250
MPVLREYLMS GGICPVSRDT IDYLLSKNGS GNAIIIVVGG AAESLSSMPG
260 270 280 290 300
KNAVTLRNRK GFVKLALRHG ADLVPIYSFG ENEVYKQVIF EEGSWGRWVQ
310 320 330 340 350
KKFQKYIGFA PCIFHGRGLF SSDTWGLVPY SKPITTVVGE PITIPKLEHP
360 370 380
TQQDIDLYHT MYMEALVKLF DKHKTKFGLP ETEVLEVN
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketS4R3S3 | S4R3S3_HUMAN | Diacylglycerol O-acyltransferase 2 | DGAT2 | 295 | Annotation score: | ||
S4R383 | S4R383_HUMAN | Diacylglycerol O-acyltransferase 2 | DGAT2 | 160 | Annotation score: | ||
S4R3Z3 | S4R3Z3_HUMAN | Diacylglycerol O-acyltransferase 2 | DGAT2 | 112 | Annotation score: | ||
S4R449 | S4R449_HUMAN | Diacylglycerol O-acyltransferase 2 | DGAT2 | 113 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 295 | W → C in AAV35727 (Ref. 7) Curated | 1 | |
Sequence conflicti | 304 | Q → H in AAV35727 (Ref. 7) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_077236 | 223 | Y → H Found in patients with Charcot-Marie-Tooth disease; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs869025595EnsemblClinVar. | 1 | |
Natural variantiVAR_033864 | 317 | R → G. Corresponds to variant dbSNP:rs34421064Ensembl. | 1 | |
Natural variantiVAR_033865 | 361 | M → I. Corresponds to variant dbSNP:rs34113941Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020356 | 41 – 83 | Missing in isoform 2. 1 PublicationAdd BLAST | 43 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF384161 mRNA Translation: AAK84176.2 AB048286 mRNA Translation: BAB40641.2 AY358532 mRNA Translation: AAQ88896.1 AL834287 mRNA Translation: CAD38961.1 Different initiation. CR749377 mRNA Translation: CAH18230.1 AP001922 Genomic DNA No translation available. BC015234 mRNA Translation: AAH15234.1 AY780647 mRNA Translation: AAV35727.1 AB073384 mRNA Translation: BAD38635.1 Different initiation. |
CCDSi | CCDS31642.1 [Q96PD7-1] CCDS58162.1 [Q96PD7-2] |
RefSeqi | NP_001240820.1, NM_001253891.1 [Q96PD7-2] NP_115953.2, NM_032564.4 [Q96PD7-1] |
Genome annotation databases
Ensembli | ENST00000228027.12; ENSP00000228027.6; ENSG00000062282.15 ENST00000376262.7; ENSP00000365438.3; ENSG00000062282.15 [Q96PD7-2] |
GeneIDi | 84649 |
KEGGi | hsa:84649 |
MANE-Selecti | ENST00000228027.12; ENSP00000228027.6; NM_032564.5; NP_115953.2 |
UCSCi | uc001oxa.4, human [Q96PD7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF384161 mRNA Translation: AAK84176.2 AB048286 mRNA Translation: BAB40641.2 AY358532 mRNA Translation: AAQ88896.1 AL834287 mRNA Translation: CAD38961.1 Different initiation. CR749377 mRNA Translation: CAH18230.1 AP001922 Genomic DNA No translation available. BC015234 mRNA Translation: AAH15234.1 AY780647 mRNA Translation: AAV35727.1 AB073384 mRNA Translation: BAD38635.1 Different initiation. |
CCDSi | CCDS31642.1 [Q96PD7-1] CCDS58162.1 [Q96PD7-2] |
RefSeqi | NP_001240820.1, NM_001253891.1 [Q96PD7-2] NP_115953.2, NM_032564.4 [Q96PD7-1] |
3D structure databases
AlphaFoldDBi | Q96PD7 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 124172, 6 interactors |
CORUMi | Q96PD7 |
IntActi | Q96PD7, 2 interactors |
MINTi | Q96PD7 |
STRINGi | 9606.ENSP00000228027 |
Chemistry databases
BindingDBi | Q96PD7 |
ChEMBLi | CHEMBL5853 |
DrugBanki | DB13961, Fish oil DB08949, Inositol nicotinate DB09568, Omega-3-carboxylic acids |
SwissLipidsi | SLP:000000304 |
PTM databases
iPTMneti | Q96PD7 |
PhosphoSitePlusi | Q96PD7 |
Genetic variation databases
BioMutai | DGAT2 |
DMDMi | 74732654 |
Proteomic databases
EPDi | Q96PD7 |
jPOSTi | Q96PD7 |
MassIVEi | Q96PD7 |
PaxDbi | Q96PD7 |
PeptideAtlasi | Q96PD7 |
PRIDEi | Q96PD7 |
Protocols and materials databases
Antibodypediai | 1632, 312 antibodies from 35 providers |
DNASUi | 84649 |
Genome annotation databases
Ensembli | ENST00000228027.12; ENSP00000228027.6; ENSG00000062282.15 ENST00000376262.7; ENSP00000365438.3; ENSG00000062282.15 [Q96PD7-2] |
GeneIDi | 84649 |
KEGGi | hsa:84649 |
MANE-Selecti | ENST00000228027.12; ENSP00000228027.6; NM_032564.5; NP_115953.2 |
UCSCi | uc001oxa.4, human [Q96PD7-1] |
Organism-specific databases
CTDi | 84649 |
DisGeNETi | 84649 |
GeneCardsi | DGAT2 |
GeneReviewsi | DGAT2 |
HGNCi | HGNC:16940, DGAT2 |
HPAi | ENSG00000062282, Group enriched (adipose tissue, breast, liver, skin) |
MalaCardsi | DGAT2 |
MIMi | 606983, gene |
neXtProti | NX_Q96PD7 |
OpenTargetsi | ENSG00000062282 |
Orphaneti | 487814, Autosomal dominant Charcot-Marie-Tooth disease type 2 due to DGAT2 mutation |
PharmGKBi | PA27304 |
VEuPathDBi | HostDB:ENSG00000062282 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT01030000234582 |
HOGENOMi | CLU_023995_0_1_1 |
InParanoidi | Q96PD7 |
OMAi | STMFYVP |
OrthoDBi | 1347007at2759 |
PhylomeDBi | Q96PD7 |
TreeFami | TF314707 |
Enzyme and pathway databases
UniPathwayi | UPA00282 |
BRENDAi | 2.3.1.20, 2681 |
PathwayCommonsi | Q96PD7 |
Reactomei | R-HSA-1482883, Acyl chain remodeling of DAG and TAG R-HSA-75109, Triglyceride biosynthesis |
SABIO-RKi | Q96PD7 |
SignaLinki | Q96PD7 |
Miscellaneous databases
BioGRID-ORCSi | 84649, 15 hits in 1081 CRISPR screens |
ChiTaRSi | DGAT2, human |
GenomeRNAii | 84649 |
Pharosi | Q96PD7, Tchem |
PROi | PR:Q96PD7 |
RNActi | Q96PD7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000062282, Expressed in upper arm skin and 178 other tissues |
ExpressionAtlasi | Q96PD7, baseline and differential |
Genevisiblei | Q96PD7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DGAT2_HUMAN | |
Accessioni | Q96PD7Primary (citable) accession number: Q96PD7 Secondary accession number(s): A6ND76 Q9BYE5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families