UniProtKB - Q96PC3 (AP1S3_HUMAN)
AP-1 complex subunit sigma-3
AP1S3
Functioni
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
- post-Golgi vesicle-mediated transport Source: Reactome
- protein targeting Source: UniProtKB
- regulation of defense response to virus by virus Source: Reactome
- vesicle-mediated transport Source: GO_Central
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q96PC3 |
Reactomei | R-HSA-164940, Nef mediated downregulation of MHC class I complex cell surface expression R-HSA-2132295, MHC class II antigen presentation R-HSA-432720, Lysosome Vesicle Biogenesis R-HSA-432722, Golgi Associated Vesicle Biogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: AP-1 complex subunit sigma-3Alternative name(s): Adaptor protein complex AP-1 subunit sigma-1C Adaptor-related protein complex 1 subunit sigma-1C Clathrin assembly protein complex 1 sigma-1C small chain Golgi adaptor HA1/AP1 adaptin sigma-1C subunit Sigma 1C subunit of AP-1 clathrin Sigma-adaptin 1C Sigma1C-adaptin |
Gene namesi | Name:AP1S3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18971, AP1S3 |
MIMi | 615781, gene |
neXtProti | NX_Q96PC3 |
VEuPathDBi | HostDB:ENSG00000152056.16 |
Subcellular locationi
Golgi apparatus
Other locations
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Cytosol
- cytosol Source: Reactome
Golgi apparatus
- Golgi membrane Source: Reactome
- trans-Golgi network membrane Source: Reactome
Lysosome
- lysosomal membrane Source: Reactome
Plasma Membrane
- clathrin-coated pit Source: UniProtKB-SubCell
Other locations
- cytoplasmic vesicle membrane Source: Reactome
- intracellular membrane-bounded organelle Source: HPA
- membrane coat Source: InterPro
Keywords - Cellular componenti
Coated pit, Cytoplasmic vesicle, Golgi apparatus, MembranePathology & Biotechi
Involvement in diseasei
Psoriasis 15, pustular (PSORS15)1 Publication
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_072545 | 4 | F → C in PSORS15; results in decreased protein levels. 1 PublicationCorresponds to variant dbSNP:rs116107386EnsemblClinVar. | 1 | |
Natural variantiVAR_072549 | 33 | R → W in PSORS15; results in decreased TLR3 targeting to the endosomes indicating impaired function. 1 PublicationCorresponds to variant dbSNP:rs138292988EnsemblClinVar. | 1 |
Keywords - Diseasei
Disease variantOrganism-specific databases
DisGeNETi | 130340 |
MalaCardsi | AP1S3 |
MIMi | 616106, phenotype |
OpenTargetsi | ENSG00000152056 |
Orphaneti | 163931, Acrodermatitis continua of Hallopeau 247353, Generalized pustular psoriasis 163927, Pustulosis palmaris et plantaris |
Miscellaneous databases
Pharosi | Q96PC3, Tbio |
Genetic variation databases
BioMutai | AP1S3 |
DMDMi | 21541959 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193801 | 1 – 154 | AP-1 complex subunit sigma-3Add BLAST | 154 |
Proteomic databases
jPOSTi | Q96PC3 |
MassIVEi | Q96PC3 |
MaxQBi | Q96PC3 |
PaxDbi | Q96PC3 |
PeptideAtlasi | Q96PC3 |
PRIDEi | Q96PC3 |
ProteomicsDBi | 77660 [Q96PC3-1] 77661 [Q96PC3-2] 77662 [Q96PC3-3] 77663 [Q96PC3-4] |
PTM databases
iPTMneti | Q96PC3 |
PhosphoSitePlusi | Q96PC3 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000152056, Expressed in corpus epididymis and 150 other tissues |
ExpressionAtlasi | Q96PC3, baseline and differential |
Genevisiblei | Q96PC3, HS |
Organism-specific databases
HPAi | ENSG00000152056, Group enriched (ductus deferens, epididymis, seminal vesicle) |
Interactioni
Subunit structurei
Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).
Binary interactionsi
Hide detailsQ96PC3
With | #Exp. | IntAct |
---|---|---|
AAGAB [Q6PD74] | 3 | EBI-3923129,EBI-719906 |
Isoform 2 [Q96PC3-2]
With | #Exp. | IntAct |
---|---|---|
AAGAB [Q6PD74] | 3 | EBI-12780485,EBI-719906 |
Protein-protein interaction databases
BioGRIDi | 126229, 16 interactors |
ComplexPortali | CPX-5049, Ubiquitous AP-1 Adaptor complex, sigma1c variant |
CORUMi | Q96PC3 |
IntActi | Q96PC3, 7 interactors |
STRINGi | 9606.ENSP00000379891 |
Miscellaneous databases
RNActi | Q96PC3, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0934, Eukaryota |
GeneTreei | ENSGT00970000193372 |
HOGENOMi | CLU_061221_4_1_1 |
InParanoidi | Q96PC3 |
OMAi | RMSSFID |
OrthoDBi | 1307450at2759 |
PhylomeDBi | Q96PC3 |
TreeFami | TF312921 |
Family and domain databases
InterProi | View protein in InterPro IPR016635, AP_complex_ssu IPR022775, AP_mu_sigma_su IPR000804, Clathrin_sm-chain_CS IPR011012, Longin-like_dom_sf |
PANTHERi | PTHR11753, PTHR11753, 1 hit |
Pfami | View protein in Pfam PF01217, Clat_adaptor_s, 1 hit |
PIRSFi | PIRSF015588, AP_complex_sigma, 1 hit |
SUPFAMi | SSF64356, SSF64356, 1 hit |
PROSITEi | View protein in PROSITE PS00989, CLAT_ADAPTOR_S, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIHFILLFSR QGKLRLQKWY ITLPDKERKK ITREIVQIIL SRGHRTSSFV
60 70 80 90 100
DWKELKLVYK RYASLYFCCA IENQDNELLT LEIVHRYVEL LDKYFGNVCE
110 120 130 140 150
LDIIFNFEKA YFILDEFIIG GEIQETSKKI AVKAIEDSDM LQEVSTVSQT
MGER
The sequence of this isoform differs from the canonical sequence as follows:
144-154: VSTVSQTMGER → NRLSPRGRDCSEPRSCHCTLA
The sequence of this isoform differs from the canonical sequence as follows:
98-104: VCELDII → TWPFARA
105-154: Missing.
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB8ZZZ1 | B8ZZZ1_HUMAN | AP complex subunit sigma | AP1S3 | 137 | Annotation score: | ||
F8W8K9 | F8W8K9_HUMAN | AP-1 complex subunit sigma-3 | AP1S3 | 63 | Annotation score: | ||
C9JZK4 | C9JZK4_HUMAN | AP-1 complex subunit sigma-3 | AP1S3 | 56 | Annotation score: | ||
G5E966 | G5E966_HUMAN | AP-1 complex subunit sigma-3 | AP1S3 hCG_21436 | 81 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_072545 | 4 | F → C in PSORS15; results in decreased protein levels. 1 PublicationCorresponds to variant dbSNP:rs116107386EnsemblClinVar. | 1 | |
Natural variantiVAR_072546 | 17 | Q → K1 PublicationCorresponds to variant dbSNP:rs750870128Ensembl. | 1 | |
Natural variantiVAR_072547 | 22 | T → A1 PublicationCorresponds to variant dbSNP:rs149183052Ensembl. | 1 | |
Natural variantiVAR_072548 | 32 | T → I1 PublicationCorresponds to variant dbSNP:rs78536455Ensembl. | 1 | |
Natural variantiVAR_072549 | 33 | R → W in PSORS15; results in decreased TLR3 targeting to the endosomes indicating impaired function. 1 PublicationCorresponds to variant dbSNP:rs138292988EnsemblClinVar. | 1 | |
Natural variantiVAR_072550 | 79 | L → V1 PublicationCorresponds to variant dbSNP:rs34353588Ensembl. | 1 | |
Natural variantiVAR_072551 | 83 | I → T1 PublicationCorresponds to variant dbSNP:rs202157374Ensembl. | 1 | |
Natural variantiVAR_072552 | 124 | Q → E1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015942 | 98 – 104 | VCELDII → TWPFARA in isoform 3. 1 Publication | 7 | |
Alternative sequenceiVSP_015943 | 105 – 154 | Missing in isoform 3. 1 PublicationAdd BLAST | 50 | |
Alternative sequenceiVSP_015944 | 144 – 154 | VSTVSQTMGER → NRLSPRGRDCSEPRSCHCTL A in isoform 2. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_040384 | 144 – 154 | VSTVSQTMGER → TMEEYMNKPTF in isoform 4. 1 PublicationAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF393369 mRNA Translation: AAL09586.1 AK299026 mRNA Translation: BAG61103.1 AC012512 Genomic DNA No translation available. AC093884 Genomic DNA No translation available. BC009606 mRNA Translation: AAH09606.1 BC021898 mRNA Translation: AAH21898.1 |
CCDSi | CCDS42827.1 [Q96PC3-4] |
RefSeqi | NP_001034658.1, NM_001039569.1 [Q96PC3-4] |
Genome annotation databases
Ensembli | ENST00000396654; ENSP00000379891; ENSG00000152056 [Q96PC3-4] ENST00000415298; ENSP00000401705; ENSG00000152056 [Q96PC3-3] ENST00000443700; ENSP00000397155; ENSG00000152056 [Q96PC3-2] ENST00000446015; ENSP00000388738; ENSG00000152056 [Q96PC3-1] |
GeneIDi | 130340 |
KEGGi | hsa:130340 |
UCSCi | uc002vnn.4, human [Q96PC3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF393369 mRNA Translation: AAL09586.1 AK299026 mRNA Translation: BAG61103.1 AC012512 Genomic DNA No translation available. AC093884 Genomic DNA No translation available. BC009606 mRNA Translation: AAH09606.1 BC021898 mRNA Translation: AAH21898.1 |
CCDSi | CCDS42827.1 [Q96PC3-4] |
RefSeqi | NP_001034658.1, NM_001039569.1 [Q96PC3-4] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4HMY | X-ray | 7.00 | S | 1-154 | [»] | |
6CM9 | electron microscopy | 3.73 | S | 1-154 | [»] | |
6CRI | electron microscopy | 6.80 | S/a/b | 1-142 | [»] | |
6D83 | electron microscopy | 4.27 | S | 1-154 | [»] | |
6D84 | electron microscopy | 6.72 | Q/S | 1-154 | [»] | |
6DFF | electron microscopy | 3.90 | S | 1-154 | [»] | |
SMRi | Q96PC3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 126229, 16 interactors |
ComplexPortali | CPX-5049, Ubiquitous AP-1 Adaptor complex, sigma1c variant |
CORUMi | Q96PC3 |
IntActi | Q96PC3, 7 interactors |
STRINGi | 9606.ENSP00000379891 |
PTM databases
iPTMneti | Q96PC3 |
PhosphoSitePlusi | Q96PC3 |
Genetic variation databases
BioMutai | AP1S3 |
DMDMi | 21541959 |
Proteomic databases
jPOSTi | Q96PC3 |
MassIVEi | Q96PC3 |
MaxQBi | Q96PC3 |
PaxDbi | Q96PC3 |
PeptideAtlasi | Q96PC3 |
PRIDEi | Q96PC3 |
ProteomicsDBi | 77660 [Q96PC3-1] 77661 [Q96PC3-2] 77662 [Q96PC3-3] 77663 [Q96PC3-4] |
Protocols and materials databases
Antibodypediai | 54195, 99 antibodies |
Genome annotation databases
Ensembli | ENST00000396654; ENSP00000379891; ENSG00000152056 [Q96PC3-4] ENST00000415298; ENSP00000401705; ENSG00000152056 [Q96PC3-3] ENST00000443700; ENSP00000397155; ENSG00000152056 [Q96PC3-2] ENST00000446015; ENSP00000388738; ENSG00000152056 [Q96PC3-1] |
GeneIDi | 130340 |
KEGGi | hsa:130340 |
UCSCi | uc002vnn.4, human [Q96PC3-1] |
Organism-specific databases
CTDi | 130340 |
DisGeNETi | 130340 |
GeneCardsi | AP1S3 |
HGNCi | HGNC:18971, AP1S3 |
HPAi | ENSG00000152056, Group enriched (ductus deferens, epididymis, seminal vesicle) |
MalaCardsi | AP1S3 |
MIMi | 615781, gene 616106, phenotype |
neXtProti | NX_Q96PC3 |
OpenTargetsi | ENSG00000152056 |
Orphaneti | 163931, Acrodermatitis continua of Hallopeau 247353, Generalized pustular psoriasis 163927, Pustulosis palmaris et plantaris |
VEuPathDBi | HostDB:ENSG00000152056.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0934, Eukaryota |
GeneTreei | ENSGT00970000193372 |
HOGENOMi | CLU_061221_4_1_1 |
InParanoidi | Q96PC3 |
OMAi | RMSSFID |
OrthoDBi | 1307450at2759 |
PhylomeDBi | Q96PC3 |
TreeFami | TF312921 |
Enzyme and pathway databases
PathwayCommonsi | Q96PC3 |
Reactomei | R-HSA-164940, Nef mediated downregulation of MHC class I complex cell surface expression R-HSA-2132295, MHC class II antigen presentation R-HSA-432720, Lysosome Vesicle Biogenesis R-HSA-432722, Golgi Associated Vesicle Biogenesis |
Miscellaneous databases
BioGRID-ORCSi | 130340, 9 hits in 996 CRISPR screens |
ChiTaRSi | AP1S3, human |
GenomeRNAii | 130340 |
Pharosi | Q96PC3, Tbio |
PROi | PR:Q96PC3 |
RNActi | Q96PC3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000152056, Expressed in corpus epididymis and 150 other tissues |
ExpressionAtlasi | Q96PC3, baseline and differential |
Genevisiblei | Q96PC3, HS |
Family and domain databases
InterProi | View protein in InterPro IPR016635, AP_complex_ssu IPR022775, AP_mu_sigma_su IPR000804, Clathrin_sm-chain_CS IPR011012, Longin-like_dom_sf |
PANTHERi | PTHR11753, PTHR11753, 1 hit |
Pfami | View protein in Pfam PF01217, Clat_adaptor_s, 1 hit |
PIRSFi | PIRSF015588, AP_complex_sigma, 1 hit |
SUPFAMi | SSF64356, SSF64356, 1 hit |
PROSITEi | View protein in PROSITE PS00989, CLAT_ADAPTOR_S, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AP1S3_HUMAN | |
Accessioni | Q96PC3Primary (citable) accession number: Q96PC3 Secondary accession number(s): B4DQZ1, Q8WTY1, Q96DD1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2002 |
Last sequence update: | December 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families