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Protein

Cytosolic 5'-nucleotidase 1B

Gene

NT5C1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels (By similarity).By similarity

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Mg2+By similarity

Activity regulationi

Activated by ADP.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei467NucleophileBy similarity1

GO - Molecular functioni

  • 5'-nucleotidase activity Source: GO_Central
  • magnesium ion binding Source: InterPro
  • nucleotide binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium

Enzyme and pathway databases

ReactomeiR-HSA-74259 Purine catabolism
SABIO-RKiQ96P26

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic 5'-nucleotidase 1B (EC:3.1.3.5)
Short name:
cN1B
Alternative name(s):
Autoimmune infertility-related protein
Cytosolic 5'-nucleotidase IB
Short name:
cN-IB
Gene namesi
Name:NT5C1B
Synonyms:AIRP
ORF Names:FKSG85
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000185013.16
HGNCiHGNC:17818 NT5C1B
MIMi610526 gene
neXtProtiNX_Q96P26

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi93034
OpenTargetsiENSG00000185013
PharmGKBiPA31800

Polymorphism and mutation databases

BioMutaiNT5C1B
DMDMi47116569

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447961 – 610Cytosolic 5'-nucleotidase 1BAdd BLAST610

Proteomic databases

PaxDbiQ96P26
PeptideAtlasiQ96P26
PRIDEiQ96P26
ProteomicsDBi77604
77605 [Q96P26-2]
77606 [Q96P26-3]
77607 [Q96P26-4]
TopDownProteomicsiQ96P26-3 [Q96P26-3]

PTM databases

DEPODiQ96P26
iPTMnetiQ96P26
PhosphoSitePlusiQ96P26

Expressioni

Tissue specificityi

Highly expressed in testis, placenta and pancreas. Detected at lower levels in heart, kidney, liver and lung.1 Publication

Gene expression databases

BgeeiENSG00000185013 Expressed in 86 organ(s), highest expression level in sperm
CleanExiHS_NT5C1B
ExpressionAtlasiQ96P26 baseline and differential
GenevisibleiQ96P26 HS

Organism-specific databases

HPAiHPA056683

Interactioni

Protein-protein interaction databases

BioGridi124995, 1 interactor
STRINGi9606.ENSP00000352904

Structurei

3D structure databases

ProteinModelPortaliQ96P26
SMRiQ96P26
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi102 – 176Ser-richAdd BLAST75
Compositional biasi157 – 201Pro-richAdd BLAST45
Compositional biasi267 – 271Poly-Gln5

Sequence similaritiesi

Belongs to the 5'-nucleotidase type 3 family.Curated

Phylogenomic databases

eggNOGiENOG410IE01 Eukaryota
ENOG410XPRD LUCA
GeneTreeiENSGT00390000017767
HOVERGENiHBG050426
KOiK01081
OMAiMEYQLNN
OrthoDBiEOG091G0EZE
PhylomeDBiQ96P26
TreeFamiTF329831

Family and domain databases

InterProiView protein in InterPro
IPR010394 5-nucleotidase
PANTHERiPTHR31367 PTHR31367, 1 hit
PfamiView protein in Pfam
PF06189 5-nucleotidase, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96P26-1) [UniParc]FASTAAdd to basket
Also known as: CN-Ib alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQTSLKQKK NEPGMRSSKE SLEAEKRKES DKTGVRLSNQ MRRAVNPNHS
60 70 80 90 100
LRCCPFQGHS SCRRCLCAAE GTALGPCHTI RIYIHMCLLW EQGQQITMMR
110 120 130 140 150
GSQESSLRKT DSRGYLVRSQ WSRISRSPST KAPSIDEPRS RNTSAKLPSS
160 170 180 190 200
STSSRTPSTS PSLHDSSPPP LSGQPSLQPP ASPQLPRSLD SRPPTPPEPD
210 220 230 240 250
PGSRRSTKMQ ENPEAWAQGI VREIRQTRDS QPLEYSRTSP TEWKSSSQRR
260 270 280 290 300
GIYPASTQLD RNSLSEQQQQ QREDEDDYEA AYWASMRSFY EKNPSCSRPW
310 320 330 340 350
PPKPKNAITI ALSSCALFNM VDGRKIYEQE GLEKYMEYQL TNENVILTPG
360 370 380 390 400
PAFRFVKALQ YVNARLRDLY PDEQDLFDIV LMTNNHAQVG VRLINSVNHY
410 420 430 440 450
GLLIDRFCLT GGKDPIGYLK AYLTNLYIAA DSEKVQEAIQ EGIASATMFD
460 470 480 490 500
GAKDMAYCDT QLRVAFDGDA VLFSDESEHF TKEHGLDKFF QYDTLCESKP
510 520 530 540 550
LAQGPLKGFL EDLGRLQKKF YAKNERLLCP IRTYLVTARS AASSGARVLK
560 570 580 590 600
TLRRWGLEID EALFLAGAPK SPILVKIRPH IFFDDHMFHI EGAQRLGSIA
610
AYGFNKKFSS
Length:610
Mass (Da):68,804
Last modified:May 10, 2004 - v2
Checksum:iEEC0BE9E5498B3E9
GO
Isoform 2 (identifier: Q96P26-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-100: Missing.

Note: No experimental confirmation available.
Show »
Length:550
Mass (Da):61,934
Checksum:iC0C913137DFD2479
GO
Isoform 3 (identifier: Q96P26-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-208: Missing.

Note: No experimental confirmation available.
Show »
Length:402
Mass (Da):45,860
Checksum:iBFF1852B61C0EA5B
GO
Isoform 4 (identifier: Q96P26-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     596-610: LGSIAAYGFNKKFSS → KSLGWMS

Note: No experimental confirmation available.
Show »
Length:602
Mass (Da):68,022
Checksum:i1D2A9C840FE9ED0D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C4AM88C4AM88_HUMAN
Cytosolic 5'-nucleotidase 1B
NT5C1B
203Annotation score:
H7BZQ4H7BZQ4_HUMAN
Cytosolic 5'-nucleotidase 1B
NT5C1B
265Annotation score:
C9JW75C9JW75_HUMAN
Cytosolic 5'-nucleotidase 1B
NT5C1B
205Annotation score:

Sequence cautioni

The sequence AAK39108 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAK83285 differs from that shown. Reason: Frameshift at position 320.Curated
The sequence BAC04103 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAC44363 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173G → R in AAL11910 (Ref. 2) Curated1
Sequence conflicti287R → K in AAK39108 (Ref. 1) Curated1
Sequence conflicti297S → W in AAK39108 (Ref. 1) Curated1
Sequence conflicti351P → Q in AAL11910 (Ref. 2) Curated1
Sequence conflicti362V → I in BAC04174 (PubMed:14702039).Curated1
Sequence conflicti528L → P in CAC44363 (PubMed:11690631).Curated1
Sequence conflicti552L → F in AAK39108 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0102021 – 208Missing in isoform 3. 1 PublicationAdd BLAST208
Alternative sequenceiVSP_01020141 – 100Missing in isoform 2. 2 PublicationsAdd BLAST60
Alternative sequenceiVSP_010203596 – 610LGSIA…KKFSS → KSLGWMS in isoform 4. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF356185 mRNA Translation: AAK39108.1 Different initiation.
AF417165 mRNA Translation: AAL11910.1
AK093234 mRNA Translation: BAC04103.1 Different initiation.
AK093464 mRNA Translation: BAC04174.1
AK057444 mRNA Translation: BAB71489.1
AC079148 Genomic DNA Translation: AAX93260.1
CH471053 Genomic DNA Translation: EAX00853.1
BC136387 mRNA Translation: AAI36388.1
BC171761 mRNA Translation: AAI71761.1
AF352326 mRNA Translation: AAK83285.1 Frameshift.
AJ295254 mRNA Translation: CAC44363.1 Different initiation.
CCDSiCCDS33149.1 [Q96P26-2]
CCDS33150.1 [Q96P26-1]
RefSeqiNP_001002006.1, NM_001002006.2 [Q96P26-1]
NP_001186015.1, NM_001199086.1
NP_001186016.1, NM_001199087.1
NP_001186017.1, NM_001199088.1
NP_001186033.1, NM_001199104.1 [Q96P26-4]
NP_150278.2, NM_033253.3 [Q96P26-2]
UniGeneiHs.744358

Genome annotation databases

EnsembliENST00000304081; ENSP00000305979; ENSG00000185013 [Q96P26-2]
ENST00000359846; ENSP00000352904; ENSG00000185013 [Q96P26-1]
GeneIDi100526794
93034
KEGGihsa:100526794
hsa:93034
UCSCiuc002rcz.4 human [Q96P26-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF356185 mRNA Translation: AAK39108.1 Different initiation.
AF417165 mRNA Translation: AAL11910.1
AK093234 mRNA Translation: BAC04103.1 Different initiation.
AK093464 mRNA Translation: BAC04174.1
AK057444 mRNA Translation: BAB71489.1
AC079148 Genomic DNA Translation: AAX93260.1
CH471053 Genomic DNA Translation: EAX00853.1
BC136387 mRNA Translation: AAI36388.1
BC171761 mRNA Translation: AAI71761.1
AF352326 mRNA Translation: AAK83285.1 Frameshift.
AJ295254 mRNA Translation: CAC44363.1 Different initiation.
CCDSiCCDS33149.1 [Q96P26-2]
CCDS33150.1 [Q96P26-1]
RefSeqiNP_001002006.1, NM_001002006.2 [Q96P26-1]
NP_001186015.1, NM_001199086.1
NP_001186016.1, NM_001199087.1
NP_001186017.1, NM_001199088.1
NP_001186033.1, NM_001199104.1 [Q96P26-4]
NP_150278.2, NM_033253.3 [Q96P26-2]
UniGeneiHs.744358

3D structure databases

ProteinModelPortaliQ96P26
SMRiQ96P26
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124995, 1 interactor
STRINGi9606.ENSP00000352904

PTM databases

DEPODiQ96P26
iPTMnetiQ96P26
PhosphoSitePlusiQ96P26

Polymorphism and mutation databases

BioMutaiNT5C1B
DMDMi47116569

Proteomic databases

PaxDbiQ96P26
PeptideAtlasiQ96P26
PRIDEiQ96P26
ProteomicsDBi77604
77605 [Q96P26-2]
77606 [Q96P26-3]
77607 [Q96P26-4]
TopDownProteomicsiQ96P26-3 [Q96P26-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304081; ENSP00000305979; ENSG00000185013 [Q96P26-2]
ENST00000359846; ENSP00000352904; ENSG00000185013 [Q96P26-1]
GeneIDi100526794
93034
KEGGihsa:100526794
hsa:93034
UCSCiuc002rcz.4 human [Q96P26-1]

Organism-specific databases

CTDi100526794
93034
DisGeNETi93034
EuPathDBiHostDB:ENSG00000185013.16
GeneCardsiNT5C1B
HGNCiHGNC:17818 NT5C1B
HPAiHPA056683
MIMi610526 gene
neXtProtiNX_Q96P26
OpenTargetsiENSG00000185013
PharmGKBiPA31800
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IE01 Eukaryota
ENOG410XPRD LUCA
GeneTreeiENSGT00390000017767
HOVERGENiHBG050426
KOiK01081
OMAiMEYQLNN
OrthoDBiEOG091G0EZE
PhylomeDBiQ96P26
TreeFamiTF329831

Enzyme and pathway databases

ReactomeiR-HSA-74259 Purine catabolism
SABIO-RKiQ96P26

Miscellaneous databases

PROiPR:Q96P26
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000185013 Expressed in 86 organ(s), highest expression level in sperm
CleanExiHS_NT5C1B
ExpressionAtlasiQ96P26 baseline and differential
GenevisibleiQ96P26 HS

Family and domain databases

InterProiView protein in InterPro
IPR010394 5-nucleotidase
PANTHERiPTHR31367 PTHR31367, 1 hit
PfamiView protein in Pfam
PF06189 5-nucleotidase, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei5NT1B_HUMAN
AccessioniPrimary (citable) accession number: Q96P26
Secondary accession number(s): B5MCR0
, B7ZVX7, Q53RX2, Q8N9W3, Q8NA26, Q96DU5, Q96KE6, Q96M25, Q96SA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 7, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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