UniProtKB - Q96P16 (RPR1A_HUMAN)
Regulation of nuclear pre-mRNA domain-containing protein 1A
RPRD1A
Functioni
Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. May act as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle.
3 PublicationsGO - Molecular functioni
- identical protein binding Source: IntAct
- RNA polymerase II complex binding Source: GO_Central
GO - Biological processi
- dephosphorylation of RNA polymerase II C-terminal domain Source: UniProtKB
- mRNA 3'-end processing Source: GO_Central
Enzyme and pathway databases
PathwayCommonsi | Q96P16 |
Reactomei | R-HSA-6807505, RNA polymerase II transcribes snRNA genes |
SignaLinki | Q96P16 |
Names & Taxonomyi
Protein namesi | Recommended name: Regulation of nuclear pre-mRNA domain-containing protein 1AAlternative name(s): Cyclin-dependent kinase inhibitor 2B-related protein p15INK4B-related protein |
Gene namesi | Name:RPRD1A Synonyms:P15RS |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25560, RPRD1A |
MIMi | 610347, gene |
neXtProti | NX_Q96P16 |
VEuPathDBi | HostDB:ENSG00000141425 |
Subcellular locationi
Nucleus
- nucleoplasm Source: Reactome
- RNA polymerase II, holoenzyme Source: UniProtKB
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 65 | D → A: Partial loss of binding to POLR2A CTD in vitro. 1 Publication | 1 | |
Mutagenesisi | 106 | R → A: Partial loss of binding to POLR2A CTD phosphorylated at 'Ser-2' in the heptad repeats in vitro. 1 Publication | 1 | |
Mutagenesisi | 114 | R → A: Partial loss of binding to POLR2A CTD in vitro. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 55197 |
OpenTargetsi | ENSG00000141425 |
PharmGKBi | PA162402042 |
Miscellaneous databases
Pharosi | Q96P16, Tbio |
Genetic variation databases
BioMutai | RPRD1A |
DMDMi | 74717048 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000311344 | 2 – 312 | Regulation of nuclear pre-mRNA domain-containing protein 1AAdd BLAST | 311 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources1 Publication | 1 | |
Modified residuei | 153 | PhosphoserineCombined sources | 1 | |
Modified residuei | 156 | PhosphoserineCombined sources | 1 | |
Modified residuei | 285 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q96P16 |
jPOSTi | Q96P16 |
MassIVEi | Q96P16 |
MaxQBi | Q96P16 |
PaxDbi | Q96P16 |
PeptideAtlasi | Q96P16 |
PRIDEi | Q96P16 |
ProteomicsDBi | 77597 [Q96P16-1] 77598 [Q96P16-3] |
PTM databases
GlyGeni | Q96P16, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96P16 |
PhosphoSitePlusi | Q96P16 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSG00000141425, Expressed in primary visual cortex and 238 other tissues |
ExpressionAtlasi | Q96P16, baseline and differential |
Genevisiblei | Q96P16, HS |
Organism-specific databases
HPAi | ENSG00000141425, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Q96P16
With | #Exp. | IntAct |
---|---|---|
KPNA3 [O00505] | 3 | EBI-1053506,EBI-358297 |
POLR2A [P24928] | 3 | EBI-1053506,EBI-295301 |
RPRD1B [Q9NQG5] | 4 | EBI-1053506,EBI-747925 |
RPRD1A - isoform 1 [Q96P16-1]
With | #Exp. | IntAct |
---|---|---|
RPAP2 [Q8IXW5] | 3 | EBI-16112633,EBI-395878 |
itself | 3 | EBI-16112633,EBI-16112633 |
RPRD1B [Q9NQG5] | 4 | EBI-16112633,EBI-747925 |
Isoform 2 [Q96P16-3]
GO - Molecular functioni
- identical protein binding Source: IntAct
- RNA polymerase II complex binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 120494, 153 interactors |
DIPi | DIP-50901N |
IntActi | Q96P16, 46 interactors |
MINTi | Q96P16 |
STRINGi | 9606.ENSP00000381984 |
Miscellaneous databases
RNActi | Q96P16, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q96P16 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 133 | CIDPROSITE-ProRule annotationAdd BLAST | 132 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 244 – 286 | Sequence analysisAdd BLAST | 43 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2669, Eukaryota |
GeneTreei | ENSGT00950000183094 |
HOGENOMi | CLU_055523_1_0_1 |
InParanoidi | Q96P16 |
OMAi | TAAQWIM |
PhylomeDBi | Q96P16 |
TreeFami | TF320926 |
Family and domain databases
Gene3Di | 1.25.40.90, 1 hit |
InterProi | View protein in InterPro IPR006569, CID_dom IPR032337, CREPT IPR008942, ENTH_VHS |
Pfami | View protein in Pfam PF04818, CID, 1 hit PF16566, CREPT, 1 hit |
SMARTi | View protein in SMART SM00582, RPR, 1 hit |
SUPFAMi | SSF48464, SSF48464, 1 hit |
PROSITEi | View protein in PROSITE PS51391, CID, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSAFSEAALE KKLSELSNSQ QSVQTLSLWL IHHRKHSRPI VTVWERELRK
60 70 80 90 100
AKPNRKLTFL YLANDVIQNS KRKGPEFTKD FAPVIVEAFK HVSSETDESC
110 120 130 140 150
KKHLGRVLSI WEERSVYEND VLEQLKQALY GDKKPRKRTY EQIKVDENEN
160 170 180 190 200
CSSLGSPSEP PQTLDLVRAL QDLENAASGD AAVHQRIASL PVEVQEVSLL
210 220 230 240 250
DKITDKESGE RLSKMVEDAC MLLADYNGRL AAEIDDRKQL TRMLADFLRC
260 270 280 290 300
QKEALAEKEH KLEEYKRKLA RVSLVRKELR SRIQSLPDLS RLPNVTGSHM
310
HLPFAGDIYS ED
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0C4DGQ6 | A0A0C4DGQ6_HUMAN | Regulation of nuclear pre-mRNA doma... | RPRD1A | 289 | Annotation score: | ||
K7EQR8 | K7EQR8_HUMAN | Regulation of nuclear pre-mRNA doma... | RPRD1A | 73 | Annotation score: | ||
K7EP82 | K7EP82_HUMAN | Regulation of nuclear pre-mRNA doma... | RPRD1A | 123 | Annotation score: | ||
K7ER50 | K7ER50_HUMAN | Regulation of nuclear pre-mRNA doma... | RPRD1A | 49 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 193 | E → D in BAA91736 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 234 | I → V in BAA91736 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_037229 | 21 | Q → H in a breast cancer sample; somatic mutation. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029531 | 1 – 51 | MSAFS…ELRKA → MRNSNWRFCQTGIYS in isoform 2. 2 PublicationsAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF419845 mRNA Translation: AAL15972.1 AK001518 mRNA Translation: BAA91736.1 AK292907 mRNA Translation: BAF85596.1 AK314569 mRNA Translation: BAG37150.1 CH471088 Genomic DNA Translation: EAX01365.1 CH471088 Genomic DNA Translation: EAX01366.1 CH471088 Genomic DNA Translation: EAX01367.1 BC000225 mRNA Translation: AAH00225.2 BC010136 mRNA Translation: AAH10136.1 Sequence problems. |
CCDSi | CCDS11917.1 [Q96P16-1] CCDS77178.1 [Q96P16-3] |
RefSeqi | NP_001290340.1, NM_001303411.1 [Q96P16-3] NP_001290341.1, NM_001303412.1 [Q96P16-3] NP_001290342.1, NM_001303413.1 NP_060640.2, NM_018170.4 [Q96P16-1] XP_011524350.1, XM_011526048.1 [Q96P16-1] XP_011524351.1, XM_011526049.1 [Q96P16-3] XP_011524352.1, XM_011526050.1 [Q96P16-3] |
Genome annotation databases
Ensembli | ENST00000357384; ENSP00000349955; ENSG00000141425 ENST00000399022; ENSP00000381984; ENSG00000141425 ENST00000588737; ENSP00000465444; ENSG00000141425 [Q96P16-3] ENST00000590898; ENSP00000467991; ENSG00000141425 [Q96P16-3] |
GeneIDi | 55197 |
KEGGi | hsa:55197 |
MANE-Selecti | ENST00000399022.9; ENSP00000381984.3; NM_018170.5; NP_060640.2 |
UCSCi | uc002kze.2, human [Q96P16-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF419845 mRNA Translation: AAL15972.1 AK001518 mRNA Translation: BAA91736.1 AK292907 mRNA Translation: BAF85596.1 AK314569 mRNA Translation: BAG37150.1 CH471088 Genomic DNA Translation: EAX01365.1 CH471088 Genomic DNA Translation: EAX01366.1 CH471088 Genomic DNA Translation: EAX01367.1 BC000225 mRNA Translation: AAH00225.2 BC010136 mRNA Translation: AAH10136.1 Sequence problems. |
CCDSi | CCDS11917.1 [Q96P16-1] CCDS77178.1 [Q96P16-3] |
RefSeqi | NP_001290340.1, NM_001303411.1 [Q96P16-3] NP_001290341.1, NM_001303412.1 [Q96P16-3] NP_001290342.1, NM_001303413.1 NP_060640.2, NM_018170.4 [Q96P16-1] XP_011524350.1, XM_011526048.1 [Q96P16-1] XP_011524351.1, XM_011526049.1 [Q96P16-3] XP_011524352.1, XM_011526050.1 [Q96P16-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4JXT | X-ray | 1.90 | A | 1-137 | [»] | |
4NAC | X-ray | 2.00 | A/B | 1-131 | [»] | |
SMRi | Q96P16 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120494, 153 interactors |
DIPi | DIP-50901N |
IntActi | Q96P16, 46 interactors |
MINTi | Q96P16 |
STRINGi | 9606.ENSP00000381984 |
PTM databases
GlyGeni | Q96P16, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96P16 |
PhosphoSitePlusi | Q96P16 |
Genetic variation databases
BioMutai | RPRD1A |
DMDMi | 74717048 |
Proteomic databases
EPDi | Q96P16 |
jPOSTi | Q96P16 |
MassIVEi | Q96P16 |
MaxQBi | Q96P16 |
PaxDbi | Q96P16 |
PeptideAtlasi | Q96P16 |
PRIDEi | Q96P16 |
ProteomicsDBi | 77597 [Q96P16-1] 77598 [Q96P16-3] |
Protocols and materials databases
ABCDi | Q96P16, 6 sequenced antibodies |
Antibodypediai | 22307, 123 antibodies from 23 providers |
DNASUi | 55197 |
Genome annotation databases
Ensembli | ENST00000357384; ENSP00000349955; ENSG00000141425 ENST00000399022; ENSP00000381984; ENSG00000141425 ENST00000588737; ENSP00000465444; ENSG00000141425 [Q96P16-3] ENST00000590898; ENSP00000467991; ENSG00000141425 [Q96P16-3] |
GeneIDi | 55197 |
KEGGi | hsa:55197 |
MANE-Selecti | ENST00000399022.9; ENSP00000381984.3; NM_018170.5; NP_060640.2 |
UCSCi | uc002kze.2, human [Q96P16-1] |
Organism-specific databases
CTDi | 55197 |
DisGeNETi | 55197 |
GeneCardsi | RPRD1A |
HGNCi | HGNC:25560, RPRD1A |
HPAi | ENSG00000141425, Low tissue specificity |
MIMi | 610347, gene |
neXtProti | NX_Q96P16 |
OpenTargetsi | ENSG00000141425 |
PharmGKBi | PA162402042 |
VEuPathDBi | HostDB:ENSG00000141425 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2669, Eukaryota |
GeneTreei | ENSGT00950000183094 |
HOGENOMi | CLU_055523_1_0_1 |
InParanoidi | Q96P16 |
OMAi | TAAQWIM |
PhylomeDBi | Q96P16 |
TreeFami | TF320926 |
Enzyme and pathway databases
PathwayCommonsi | Q96P16 |
Reactomei | R-HSA-6807505, RNA polymerase II transcribes snRNA genes |
SignaLinki | Q96P16 |
Miscellaneous databases
BioGRID-ORCSi | 55197, 37 hits in 1052 CRISPR screens |
ChiTaRSi | RPRD1A, human |
GenomeRNAii | 55197 |
Pharosi | Q96P16, Tbio |
PROi | PR:Q96P16 |
RNActi | Q96P16, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000141425, Expressed in primary visual cortex and 238 other tissues |
ExpressionAtlasi | Q96P16, baseline and differential |
Genevisiblei | Q96P16, HS |
Family and domain databases
Gene3Di | 1.25.40.90, 1 hit |
InterProi | View protein in InterPro IPR006569, CID_dom IPR032337, CREPT IPR008942, ENTH_VHS |
Pfami | View protein in Pfam PF04818, CID, 1 hit PF16566, CREPT, 1 hit |
SMARTi | View protein in SMART SM00582, RPR, 1 hit |
SUPFAMi | SSF48464, SSF48464, 1 hit |
PROSITEi | View protein in PROSITE PS51391, CID, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RPR1A_HUMAN | |
Accessioni | Q96P16Primary (citable) accession number: Q96P16 Secondary accession number(s): A8KA42 Q9NVL4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - Human chromosome 18
Human chromosome 18: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families