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Protein

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1

Gene

WFIKKN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei122 – 123Reactive bondPROSITE-ProRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMetalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.953

Names & Taxonomyi

Protein namesi
Recommended name:
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1
Alternative name(s):
Growth and differentiation factor-associated serum protein 2
Short name:
GASP-2
Short name:
hGASP-2
WAP, follistatin, immunoglobulin, Kunitz and NTR domain-containing protein
Gene namesi
Name:WFIKKN1
Synonyms:C16orf12, GASP2, WFIKKN
ORF Names:PSEC0040
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000127578.6
HGNCiHGNC:30912 WFIKKN1
MIMi608021 gene
neXtProtiNX_Q96NZ8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000127578
PharmGKBiPA134897686

Polymorphism and mutation databases

BioMutaiWFIKKN1
DMDMi74717044

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000030781620 – 548WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1Add BLAST529

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 66By similarity
Disulfide bondi49 ↔ 70By similarity
Disulfide bondi53 ↔ 65By similarity
Disulfide bondi59 ↔ 75By similarity
Disulfide bondi116 ↔ 146By similarity
Disulfide bondi120 ↔ 139By similarity
Disulfide bondi128 ↔ 157By similarity
Disulfide bondi207 ↔ 263By similarity
Disulfide bondi299 ↔ 351By similarity
Disulfide bondi306 ↔ 334By similarity
Disulfide bondi326 ↔ 347By similarity
Disulfide bondi359 ↔ 409By similarity
Disulfide bondi368 ↔ 392By similarity
Disulfide bondi384 ↔ 405By similarity
Disulfide bondi417 ↔ 489By similarity
Disulfide bondi420 ↔ 491By similarity
Disulfide bondi431 ↔ 540By similarity
Glycosylationi493N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ96NZ8
PaxDbiQ96NZ8
PeptideAtlasiQ96NZ8
PRIDEiQ96NZ8
ProteomicsDBi77582
TopDownProteomicsiQ96NZ8

PTM databases

iPTMnetiQ96NZ8
PhosphoSitePlusiQ96NZ8

Expressioni

Tissue specificityi

Expressed in pancreas, kidney, liver, placenta, and lung.

Gene expression databases

BgeeiENSG00000127578 Expressed in 87 organ(s), highest expression level in cerebellum
CleanExiHS_WFIKKN1
GenevisibleiQ96NZ8 HS

Organism-specific databases

HPAiCAB025858
HPA043362
HPA044237

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MSTNO147934EBI-2363713,EBI-8542977

GO - Molecular functioni

Protein-protein interaction databases

BioGridi125565, 6 interactors
IntActiQ96NZ8, 10 interactors
MINTiQ96NZ8
STRINGi9606.ENSP00000324763

Structurei

Secondary structure

1548
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96NZ8
SMRiQ96NZ8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96NZ8

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 79WAPPROSITE-ProRule annotationAdd BLAST54
Domaini108 – 159Kazal-likePROSITE-ProRule annotationAdd BLAST52
Domaini186 – 279Ig-like C2-typeAdd BLAST94
Domaini299 – 351BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd BLAST53
Domaini359 – 409BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd BLAST51
Domaini409 – 540NTRPROSITE-ProRule annotationAdd BLAST132

Domaini

The second BPTI/Kunitz inhibitor domain is able to inhibit trypsin. It has however no activity toward chymotrypsin, elastase, plasmin, pancreatic kallikrein, lung tryptase, plasma kallikrein, thrombin, urokinase or tissue plasminogen activator.1 Publication

Sequence similaritiesi

Belongs to the WFIKKN family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG4597 Eukaryota
ENOG41102GD LUCA
GeneTreeiENSGT00740000114929
HOGENOMiHOG000154855
HOVERGENiHBG108676
InParanoidiQ96NZ8
OMAiQRENLIM
OrthoDBiEOG091G09P2
PhylomeDBiQ96NZ8
TreeFamiTF315349

Family and domain databases

CDDicd00109 KU, 1 hit
Gene3Di2.60.40.10, 1 hit
4.10.410.10, 2 hits
4.10.75.10, 1 hit
InterProiView protein in InterPro
IPR036645 Elafin-like_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR001134 Netrin_domain
IPR020901 Prtase_inh_Kunz-CS
IPR008993 TIMP-like_OB-fold
IPR008197 WAP_dom
IPR033638 WFIKKN1
PANTHERiPTHR10083:SF212 PTHR10083:SF212, 1 hit
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 2 hits
PF00095 WAP, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00408 IGc2, 1 hit
SM00131 KU, 2 hits
SM00217 WAP, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
SSF48726 SSF48726, 1 hit
SSF50242 SSF50242, 1 hit
SSF57256 SSF57256, 1 hit
SSF57362 SSF57362, 2 hits
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 2 hits
PS50279 BPTI_KUNITZ_2, 2 hits
PS50835 IG_LIKE, 1 hit
PS51465 KAZAL_2, 1 hit
PS50189 NTR, 1 hit
PS51390 WAP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96NZ8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPALRPLLPL LLLLRLTSGA GLLPGLGSHP GVCPNQLSPN LWVDAQSTCE
60 70 80 90 100
RECSRDQDCA AAEKCCINVC GLHSCVAARF PGSPAAPTTA ASCEGFVCPQ
110 120 130 140 150
QGSDCDIWDG QPVCRCRDRC EKEPSFTCAS DGLTYYNRCY MDAEACLRGL
160 170 180 190 200
HLHIVPCKHV LSWPPSSPGP PETTARPTPG AAPVPPALYS SPSPQAVQVG
210 220 230 240 250
GTASLHCDVS GRPPPAVTWE KQSHQRENLI MRPDQMYGNV VVTSIGQLVL
260 270 280 290 300
YNARPEDAGL YTCTARNAAG LLRADFPLSV VQREPARDAA PSIPAPAECL
310 320 330 340 350
PDVQACTGPT SPHLVLWHYD PQRGGCMTFP ARGCDGAARG FETYEACQQA
360 370 380 390 400
CARGPGDACV LPAVQGPCRG WEPRWAYSPL LQQCHPFVYG GCEGNGNNFH
410 420 430 440 450
SRESCEDACP VPRTPPCRAC RLRSKLALSL CRSDFAIVGR LTEVLEEPEA
460 470 480 490 500
AGGIARVALE DVLKDDKMGL KFLGTKYLEV TLSGMDWACP CPNMTAGDGP
510 520 530 540
LVIMGEVRDG VAVLDAGSYV RAASEKRVKK ILELLEKQAC ELLNRFQD
Length:548
Mass (Da):58,798
Last modified:December 1, 2001 - v1
Checksum:i72BB28708D3EFB1F
GO

Sequence cautioni

The sequence AAK61237 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAC11566 differs from that shown. Reason: Frameshift at position 147.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF422194 mRNA Translation: AAL18839.1
AK075356 mRNA Translation: BAC11566.1 Frameshift.
AE006464 Genomic DNA Translation: AAK61237.1 Sequence problems.
BC101602 mRNA Translation: AAI01603.1
BC101606 mRNA Translation: AAI01607.1
CCDSiCCDS10414.1
RefSeqiNP_444514.1, NM_053284.2
UniGeneiHs.345818

Genome annotation databases

EnsembliENST00000319070; ENSP00000324763; ENSG00000127578
GeneIDi117166
KEGGihsa:117166
UCSCiuc002cht.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF422194 mRNA Translation: AAL18839.1
AK075356 mRNA Translation: BAC11566.1 Frameshift.
AE006464 Genomic DNA Translation: AAK61237.1 Sequence problems.
BC101602 mRNA Translation: AAI01603.1
BC101606 mRNA Translation: AAI01607.1
CCDSiCCDS10414.1
RefSeqiNP_444514.1, NM_053284.2
UniGeneiHs.345818

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DDINMR-A357-411[»]
2DDJNMR-A357-411[»]
ProteinModelPortaliQ96NZ8
SMRiQ96NZ8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125565, 6 interactors
IntActiQ96NZ8, 10 interactors
MINTiQ96NZ8
STRINGi9606.ENSP00000324763

Protein family/group databases

MEROPSiI02.953

PTM databases

iPTMnetiQ96NZ8
PhosphoSitePlusiQ96NZ8

Polymorphism and mutation databases

BioMutaiWFIKKN1
DMDMi74717044

Proteomic databases

EPDiQ96NZ8
PaxDbiQ96NZ8
PeptideAtlasiQ96NZ8
PRIDEiQ96NZ8
ProteomicsDBi77582
TopDownProteomicsiQ96NZ8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000319070; ENSP00000324763; ENSG00000127578
GeneIDi117166
KEGGihsa:117166
UCSCiuc002cht.2 human

Organism-specific databases

CTDi117166
EuPathDBiHostDB:ENSG00000127578.6
GeneCardsiWFIKKN1
HGNCiHGNC:30912 WFIKKN1
HPAiCAB025858
HPA043362
HPA044237
MIMi608021 gene
neXtProtiNX_Q96NZ8
OpenTargetsiENSG00000127578
PharmGKBiPA134897686
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4597 Eukaryota
ENOG41102GD LUCA
GeneTreeiENSGT00740000114929
HOGENOMiHOG000154855
HOVERGENiHBG108676
InParanoidiQ96NZ8
OMAiQRENLIM
OrthoDBiEOG091G09P2
PhylomeDBiQ96NZ8
TreeFamiTF315349

Miscellaneous databases

ChiTaRSiWFIKKN1 human
EvolutionaryTraceiQ96NZ8
GeneWikiiWFIKKN1
GenomeRNAii117166
PROiPR:Q96NZ8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000127578 Expressed in 87 organ(s), highest expression level in cerebellum
CleanExiHS_WFIKKN1
GenevisibleiQ96NZ8 HS

Family and domain databases

CDDicd00109 KU, 1 hit
Gene3Di2.60.40.10, 1 hit
4.10.410.10, 2 hits
4.10.75.10, 1 hit
InterProiView protein in InterPro
IPR036645 Elafin-like_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR001134 Netrin_domain
IPR020901 Prtase_inh_Kunz-CS
IPR008993 TIMP-like_OB-fold
IPR008197 WAP_dom
IPR033638 WFIKKN1
PANTHERiPTHR10083:SF212 PTHR10083:SF212, 1 hit
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 2 hits
PF00095 WAP, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00408 IGc2, 1 hit
SM00131 KU, 2 hits
SM00217 WAP, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
SSF48726 SSF48726, 1 hit
SSF50242 SSF50242, 1 hit
SSF57256 SSF57256, 1 hit
SSF57362 SSF57362, 2 hits
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 2 hits
PS50279 BPTI_KUNITZ_2, 2 hits
PS50835 IG_LIKE, 1 hit
PS51465 KAZAL_2, 1 hit
PS50189 NTR, 1 hit
PS51390 WAP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWFKN1_HUMAN
AccessioniPrimary (citable) accession number: Q96NZ8
Secondary accession number(s): Q7LDW0, Q8NBQ1, Q96S20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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