UniProtKB - Q96NY7 (CLIC6_HUMAN)
Protein
Chloride intracellular channel protein 6
Gene
CLIC6
Organism
Homo sapiens (Human)
Status
Functioni
May insert into membranes and form chloride ion channels. May play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport (By similarity).By similarity
GO - Molecular functioni
- chloride channel activity Source: UniProtKB-KW
- D2 dopamine receptor binding Source: GO_Central
- D3 dopamine receptor binding Source: GO_Central
- D4 dopamine receptor binding Source: GO_Central
- voltage-gated ion channel activity Source: UniProtKB-KW
GO - Biological processi
- regulation of ion transmembrane transport Source: UniProtKB-KW
Keywordsi
Molecular function | Chloride channel, Ion channel, Voltage-gated channel |
Biological process | Ion transport, Transport |
Ligand | Chloride |
Enzyme and pathway databases
PathwayCommonsi | Q96NY7 |
Protein family/group databases
TCDBi | 1.A.12.1.4, the intracellular chloride channel (clic) family |
Names & Taxonomyi
Protein namesi | Recommended name: Chloride intracellular channel protein 6Alternative name(s): Parchorin |
Gene namesi | Name:CLIC6 Synonyms:CLIC1L |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2065, CLIC6 |
MIMi | 615321, gene |
neXtProti | NX_Q96NY7 |
VEuPathDBi | HostDB:ENSG00000159212.12 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass membrane protein By similarity
Other locations
- Cytoplasm By similarity
Note: Predominantly cytoplasmic. Upon chloride ion efflux from the cell, it is translocated to the plasma membrane (By similarity).By similarity
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- chloride channel complex Source: UniProtKB-KW
- cytoplasm Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 489 – 509 | Helical; Note=After insertion into the membraneSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 54102 |
OpenTargetsi | ENSG00000159212 |
PharmGKBi | PA26593 |
Miscellaneous databases
Pharosi | Q96NY7, Tbio |
Genetic variation databases
BioMutai | CLIC6 |
DMDMi | 38372885 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144217 | 1 – 704 | Chloride intracellular channel protein 6Add BLAST | 704 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 44 | PhosphoserineBy similarity | 1 | |
Modified residuei | 397 | PhosphoserineBy similarity | 1 | |
Modified residuei | 442 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q96NY7 |
MassIVEi | Q96NY7 |
MaxQBi | Q96NY7 |
PaxDbi | Q96NY7 |
PeptideAtlasi | Q96NY7 |
PRIDEi | Q96NY7 |
ProteomicsDBi | 77576 [Q96NY7-1] 77577 [Q96NY7-2] |
PTM databases
iPTMneti | Q96NY7 |
PhosphoSitePlusi | Q96NY7 |
Expressioni
Tissue specificityi
Expressed in brain, placenta, pancreas and liver.1 Publication
Gene expression databases
Bgeei | ENSG00000159212, Expressed in pigmented layer of retina and 159 other tissues |
Genevisiblei | Q96NY7, HS |
Organism-specific databases
HPAi | ENSG00000159212, Group enriched (brain, retina, stomach) |
Interactioni
Subunit structurei
Interacts with dopamine receptors DRD2, DRD3 and DRD4.
By similarityGO - Molecular functioni
- D2 dopamine receptor binding Source: GO_Central
- D3 dopamine receptor binding Source: GO_Central
- D4 dopamine receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 119900, 4 interactors |
IntActi | Q96NY7, 1 interactor |
STRINGi | 9606.ENSP00000290332 |
Miscellaneous databases
RNActi | Q96NY7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 157 – 166 | 1 | 10 | |
Repeati | 167 – 176 | 2 | 10 | |
Repeati | 177 – 186 | 3 | 10 | |
Repeati | 187 – 196 | 4 | 10 | |
Repeati | 197 – 206 | 5 | 10 | |
Repeati | 207 – 216 | 6 | 10 | |
Repeati | 217 – 226 | 7 | 10 | |
Repeati | 227 – 236 | 8 | 10 | |
Repeati | 237 – 246 | 9 | 10 | |
Repeati | 247 – 256 | 10 | 10 | |
Repeati | 257 – 266 | 11 | 10 | |
Repeati | 267 – 276 | 12 | 10 | |
Repeati | 277 – 286 | 13 | 10 | |
Domaini | 556 – 704 | GST C-terminalAdd BLAST | 149 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 157 – 282 | 13 X 10 AA tandem repeat of G-D-[SNG]-[VIM]-[DEQ]-A-[EAG]-[GDVE]-[PRG]-[LAVP]Add BLAST | 126 |
Domaini
Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity
Sequence similaritiesi
Belongs to the chloride channel CLIC family.Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1422, Eukaryota |
GeneTreei | ENSGT00940000159602 |
HOGENOMi | CLU_023994_0_0_1 |
InParanoidi | Q96NY7 |
OMAi | QGASMEA |
OrthoDBi | 974249at2759 |
PhylomeDBi | Q96NY7 |
TreeFami | TF315438 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR002946, CLIC IPR030257, CLIC-4/6 IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR45476:SF1, PTHR45476:SF1, 2 hits |
PRINTSi | PR01263, INTCLCHANNEL |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00862, O-ClC, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform B (identifier: Q96NY7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEAAEPEGV APGPQGPPEV PAPLAERPGE PGAAGGEAEG PEGSEGAEEA
60 70 80 90 100
PRGAAAVKEA GGGGPDRGPE AEARGTRGAH GETEAEEGAP EGAEVPQGGE
110 120 130 140 150
ETSGAQQVEG ASPGRGAQGE PRGEAQREPE DSAAPERQEE AEQRPEVPEG
160 170 180 190 200
SASGEAGDSV DAEGPLGDNI EAEGPAGDSV EAEGRVGDSV DAEGPAGDSV
210 220 230 240 250
DAEGPLGDNI QAEGPAGDSV DAEGRVGDSV DAEGPAGDSV DAEGRVGDSV
260 270 280 290 300
EAGDPAGDGV EAGVPAGDSV EAEGPAGDSM DAEGPAGRAR RVSGEPQQSG
310 320 330 340 350
DGSLSPQAEA IEVAAGESAG RSPGELAWDA AEEAEVPGVK GSEEAAPGDA
360 370 380 390 400
RADAGEDRVG DGPQQEPGED EERRERSPEG PREEEAAGGE EESPDSSPHG
410 420 430 440 450
EASRGAAEPE AQLSNHLAEE GPAEGSGEAA RVNGRREDGE ASEPRALGQE
460 470 480 490 500
HDITLFVKVK LTALGCSRIA IKKYLRAGYD GESIGNCPFS QRLFMILWLK
510 520 530 540 550
GVIFNVTTVD LKRKPADLQN LAPGTNPPFM TFDGEVKTDV NKIEEFLEEK
560 570 580 590 600
LAPPRYPKLG TQHPESNSAG NDVFAKFSAF IKNTKKDANE IHEKNLLKAL
610 620 630 640 650
RKLDNYLNSP LPDEIDAYST EDVTVSGRKF LDGDELTLAD CNLLPKLHII
660 670 680 690 700
KIVAKKYRDF EFPSEMTGIW RYLNNAYARD EFTNTCPADQ EIEHAYSDVA
KRMK
Sequence cautioni
The sequence BAC03959 differs from that shown. Reason: Erroneous initiation.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_014139 | 632 | D → G. Corresponds to variant dbSNP:rs3171439Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008963 | 459 – 476 | Missing in isoform A. 4 PublicationsAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF448439 mRNA Translation: AAN76730.1 AF448438 mRNA Translation: AAN76729.1 AF426169 mRNA Translation: AAL24813.1 AK092733 mRNA Translation: BAC03959.1 Different initiation. AK289663 mRNA Translation: BAF82352.1 AP001720 Genomic DNA No translation available. CH471079 Genomic DNA Translation: EAX09775.1 BC040196 mRNA Translation: AAH40196.1 |
CCDSi | CCDS13638.1 [Q96NY7-2] CCDS82669.1 [Q96NY7-1] |
RefSeqi | NP_001303938.1, NM_001317009.1 [Q96NY7-1] NP_444507.1, NM_053277.2 [Q96NY7-2] |
Genome annotation databases
Ensembli | ENST00000349499; ENSP00000290332; ENSG00000159212 [Q96NY7-2] ENST00000360731; ENSP00000353959; ENSG00000159212 [Q96NY7-1] |
GeneIDi | 54102 |
KEGGi | hsa:54102 |
UCSCi | uc002yuf.2, human [Q96NY7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF448439 mRNA Translation: AAN76730.1 AF448438 mRNA Translation: AAN76729.1 AF426169 mRNA Translation: AAL24813.1 AK092733 mRNA Translation: BAC03959.1 Different initiation. AK289663 mRNA Translation: BAF82352.1 AP001720 Genomic DNA No translation available. CH471079 Genomic DNA Translation: EAX09775.1 BC040196 mRNA Translation: AAH40196.1 |
CCDSi | CCDS13638.1 [Q96NY7-2] CCDS82669.1 [Q96NY7-1] |
RefSeqi | NP_001303938.1, NM_001317009.1 [Q96NY7-1] NP_444507.1, NM_053277.2 [Q96NY7-2] |
3D structure databases
SMRi | Q96NY7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 119900, 4 interactors |
IntActi | Q96NY7, 1 interactor |
STRINGi | 9606.ENSP00000290332 |
Protein family/group databases
TCDBi | 1.A.12.1.4, the intracellular chloride channel (clic) family |
PTM databases
iPTMneti | Q96NY7 |
PhosphoSitePlusi | Q96NY7 |
Genetic variation databases
BioMutai | CLIC6 |
DMDMi | 38372885 |
Proteomic databases
jPOSTi | Q96NY7 |
MassIVEi | Q96NY7 |
MaxQBi | Q96NY7 |
PaxDbi | Q96NY7 |
PeptideAtlasi | Q96NY7 |
PRIDEi | Q96NY7 |
ProteomicsDBi | 77576 [Q96NY7-1] 77577 [Q96NY7-2] |
Protocols and materials databases
Antibodypediai | 8114, 189 antibodies |
Genome annotation databases
Ensembli | ENST00000349499; ENSP00000290332; ENSG00000159212 [Q96NY7-2] ENST00000360731; ENSP00000353959; ENSG00000159212 [Q96NY7-1] |
GeneIDi | 54102 |
KEGGi | hsa:54102 |
UCSCi | uc002yuf.2, human [Q96NY7-1] |
Organism-specific databases
CTDi | 54102 |
DisGeNETi | 54102 |
GeneCardsi | CLIC6 |
HGNCi | HGNC:2065, CLIC6 |
HPAi | ENSG00000159212, Group enriched (brain, retina, stomach) |
MIMi | 615321, gene |
neXtProti | NX_Q96NY7 |
OpenTargetsi | ENSG00000159212 |
PharmGKBi | PA26593 |
VEuPathDBi | HostDB:ENSG00000159212.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1422, Eukaryota |
GeneTreei | ENSGT00940000159602 |
HOGENOMi | CLU_023994_0_0_1 |
InParanoidi | Q96NY7 |
OMAi | QGASMEA |
OrthoDBi | 974249at2759 |
PhylomeDBi | Q96NY7 |
TreeFami | TF315438 |
Enzyme and pathway databases
PathwayCommonsi | Q96NY7 |
Miscellaneous databases
BioGRID-ORCSi | 54102, 3 hits in 875 CRISPR screens |
ChiTaRSi | CLIC6, human |
GeneWikii | CLIC6 |
GenomeRNAii | 54102 |
Pharosi | Q96NY7, Tbio |
PROi | PR:Q96NY7 |
RNActi | Q96NY7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000159212, Expressed in pigmented layer of retina and 159 other tissues |
Genevisiblei | Q96NY7, HS |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR002946, CLIC IPR030257, CLIC-4/6 IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR45476:SF1, PTHR45476:SF1, 2 hits |
PRINTSi | PR01263, INTCLCHANNEL |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00862, O-ClC, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLIC6_HUMAN | |
Accessioni | Q96NY7Primary (citable) accession number: Q96NY7 Secondary accession number(s): A8K0U8, Q8IX31 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2002 |
Last sequence update: | November 14, 2003 | |
Last modified: | February 10, 2021 | |
This is version 171 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 21
Human chromosome 21: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families