UniProtKB - Q96NN9 (AIFM3_HUMAN)
Protein
Apoptosis-inducing factor 3
Gene
AIFM3
Organism
Homo sapiens (Human)
Status
Functioni
Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 109 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 111 | Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 128 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 131 | Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation | 1 | |
Binding sitei | 235 | FADSequence analysis | 1 | |
Binding sitei | 240 | FADSequence analysis | 1 | |
Binding sitei | 270 | FAD; via amide nitrogen and carbonyl oxygenSequence analysis | 1 | |
Binding sitei | 467 | FADSequence analysis | 1 | |
Binding sitei | 514 | FAD; via carbonyl oxygenSequence analysis | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 201 – 205 | FADSequence analysis | 5 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- flavin adenine dinucleotide binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- oxidoreductase activity Source: UniProtKB-KW
GO - Biological processi
- execution phase of apoptosis Source: BHF-UCL
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Apoptosis, Electron transport, Transport |
Ligand | 2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q96NN9 |
Names & Taxonomyi
Protein namesi | Recommended name: Apoptosis-inducing factor 3 (EC:1.-.-.-)Alternative name(s): Apoptosis-inducing factor-like protein |
Gene namesi | Name:AIFM3 Synonyms:AIFL |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26398, AIFM3 |
MIMi | 617298, gene |
neXtProti | NX_Q96NN9 |
VEuPathDBi | HostDB:ENSG00000183773.15 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Note: Does not translocate to the nucleus upon induction of apoptosis.
Cytosol
- cytosol Source: HPA
Endoplasmic reticulum
- endoplasmic reticulum Source: HGNC-UCL
Mitochondrion
- mitochondrial inner membrane Source: HGNC-UCL
- mitochondrion Source: HGNC-UCL
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Organism-specific databases
DisGeNETi | 150209 |
OpenTargetsi | ENSG00000183773 |
PharmGKBi | PA162376173 |
Miscellaneous databases
Pharosi | Q96NN9, Tbio |
Genetic variation databases
BioMutai | AIFM3 |
DMDMi | 74732608 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000255660 | 1 – 605 | Apoptosis-inducing factor 3Add BLAST | 605 |
Proteomic databases
MassIVEi | Q96NN9 |
PaxDbi | Q96NN9 |
PeptideAtlasi | Q96NN9 |
PRIDEi | Q96NN9 |
ProteomicsDBi | 77540 [Q96NN9-1] 77541 [Q96NN9-2] 77542 [Q96NN9-3] |
PTM databases
iPTMneti | Q96NN9 |
PhosphoSitePlusi | Q96NN9 |
Expressioni
Tissue specificityi
Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000183773, Expressed in mucosa of transverse colon and 135 other tissues |
ExpressionAtlasi | Q96NN9, baseline and differential |
Genevisiblei | Q96NN9, HS |
Organism-specific databases
HPAi | ENSG00000183773, Group enriched (brain, intestine) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 127270, 9 interactors |
IntActi | Q96NN9, 6 interactors |
MINTi | Q96NN9 |
STRINGi | 9606.ENSP00000382120 |
Miscellaneous databases
RNActi | Q96NN9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 70 – 165 | RieskePROSITE-ProRule annotationAdd BLAST | 96 |
Domaini
The Rieske domain induces apoptosis.
Sequence similaritiesi
Belongs to the FAD-dependent oxidoreductase family.Curated
Phylogenomic databases
eggNOGi | KOG1336, Eukaryota |
GeneTreei | ENSGT00940000160448 |
InParanoidi | Q96NN9 |
OMAi | GAQLRYC |
OrthoDBi | 405030at2759 |
PhylomeDBi | Q96NN9 |
TreeFami | TF314028 |
Family and domain databases
Gene3Di | 2.102.10.10, 1 hit 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR028202, Reductase_C IPR017941, Rieske_2Fe-2S IPR036922, Rieske_2Fe-2S_sf |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit PF14759, Reductase_C, 1 hit PF00355, Rieske, 1 hit |
SUPFAMi | SSF50022, SSF50022, 1 hit SSF51905, SSF51905, 1 hit SSF55424, SSF55424, 1 hit |
PROSITEi | View protein in PROSITE PS51296, RIESKE, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96NN9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGGCFSKPKP VELKIEVVLP EKERGKEELS ASGKGSPRAY QGNGTARHFH
60 70 80 90 100
TEERLSTPHP YPSPQDCVEA AVCHVKDLEN GQMREVELGW GKVLLVKDNG
110 120 130 140 150
EFHALGHKCP HYGAPLVKGV LSRGRVRCPW HGACFNISTG DLEDFPGLDS
160 170 180 190 200
LHKFQVKIEK EKVYVRASKQ ALQLQRRTKV MAKCISPSAG YSSSTNVLIV
210 220 230 240 250
GAGAAGLVCA ETLRQEGFSD RIVLCTLDRH LPYDRPKLSK SLDTQPEQLA
260 270 280 290 300
LRPKEFFRAY GIEVLTEAQV VTVDVRTKKV VFKDGFKLEY SKLLLAPGSS
310 320 330 340 350
PKTLSCKGKE VENVFTIRTP EDANRVVRLA RGRNVVVVGA GFLGMEVAAY
360 370 380 390 400
LTEKAHSVSV VELEETPFRR FLGERVGRAL MKMFENNRVK FYMQTEVSEL
410 420 430 440 450
RGQEGKLKEV VLKSSKVVRA DVCVVGIGAV PATGFLRQSG IGLDSRGFIP
460 470 480 490 500
VNKMMQTNVP GVFAAGDAVT FPLAWRNNRK VNIPHWQMAH AQGRVAAQNM
510 520 530 540 550
LAQEAEMSTV PYLWTAMFGK SLRYAGYGEG FDDVIIQGDL EELKFVAFYT
560 570 580 590 600
KGDEVIAVAS MNYDPIVSKV AEVLASGRAI RKREVELFVL HSKTGDMSWL
TGKGS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9K029 | C9K029_HUMAN | Apoptosis-inducing factor 3 | AIFM3 | 269 | Annotation score: | ||
C9JPU8 | C9JPU8_HUMAN | Apoptosis-inducing factor 3 | AIFM3 | 129 | Annotation score: | ||
F8WFB6 | F8WFB6_HUMAN | Apoptosis-inducing factor 3 | AIFM3 | 121 | Annotation score: | ||
F8WC30 | F8WC30_HUMAN | Apoptosis-inducing factor 3 | AIFM3 | 74 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 165 | V → A in BAC04434 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_061553 | 508 | S → T. Corresponds to variant dbSNP:rs61356271Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_021300 | 10 | P → PGAALPT in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_021303 | 587 – 593 | Missing in isoform 2 and isoform 3. 2 Publications | 7 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR456342 mRNA Translation: CAG30228.1 AK055035 mRNA Translation: BAB70841.1 AK094844 mRNA Translation: BAC04434.1 AC002470 Genomic DNA No translation available. CH471176 Genomic DNA Translation: EAX02924.1 CH471176 Genomic DNA Translation: EAX02925.1 CH471176 Genomic DNA Translation: EAX02926.1 CH471176 Genomic DNA Translation: EAX02927.1 CH471176 Genomic DNA Translation: EAX02930.1 CH471176 Genomic DNA Translation: EAX02931.1 BC032485 mRNA Translation: AAH32485.1 |
CCDSi | CCDS13786.1 [Q96NN9-1] CCDS33605.1 [Q96NN9-3] CCDS54503.1 [Q96NN9-2] |
RefSeqi | NP_001018070.1, NM_001018060.2 [Q96NN9-3] NP_001139760.1, NM_001146288.1 [Q96NN9-2] NP_653305.1, NM_144704.2 [Q96NN9-1] |
Genome annotation databases
Ensembli | ENST00000399163; ENSP00000382116; ENSG00000183773 [Q96NN9-3] ENST00000399167; ENSP00000382120; ENSG00000183773 [Q96NN9-1] ENST00000405089; ENSP00000385800; ENSG00000183773 [Q96NN9-2] ENST00000440238; ENSP00000390798; ENSG00000183773 [Q96NN9-1] |
GeneIDi | 150209 |
KEGGi | hsa:150209 |
UCSCi | uc002ztj.3, human [Q96NN9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR456342 mRNA Translation: CAG30228.1 AK055035 mRNA Translation: BAB70841.1 AK094844 mRNA Translation: BAC04434.1 AC002470 Genomic DNA No translation available. CH471176 Genomic DNA Translation: EAX02924.1 CH471176 Genomic DNA Translation: EAX02925.1 CH471176 Genomic DNA Translation: EAX02926.1 CH471176 Genomic DNA Translation: EAX02927.1 CH471176 Genomic DNA Translation: EAX02930.1 CH471176 Genomic DNA Translation: EAX02931.1 BC032485 mRNA Translation: AAH32485.1 |
CCDSi | CCDS13786.1 [Q96NN9-1] CCDS33605.1 [Q96NN9-3] CCDS54503.1 [Q96NN9-2] |
RefSeqi | NP_001018070.1, NM_001018060.2 [Q96NN9-3] NP_001139760.1, NM_001146288.1 [Q96NN9-2] NP_653305.1, NM_144704.2 [Q96NN9-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5O9V | X-ray | 2.20 | C/D | 2-9 | [»] | |
6QRM | X-ray | 2.30 | C/D | 2-11 | [»] | |
6SJZ | X-ray | 2.00 | E/F | 2-9 | [»] | |
6SK3 | X-ray | 2.70 | C/D | 2-9 | [»] | |
6SK8 | X-ray | 1.87 | C/D | 2-9 | [»] | |
6SKJ | X-ray | 2.80 | C/D | 2-9 | [»] | |
SMRi | Q96NN9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 127270, 9 interactors |
IntActi | Q96NN9, 6 interactors |
MINTi | Q96NN9 |
STRINGi | 9606.ENSP00000382120 |
PTM databases
iPTMneti | Q96NN9 |
PhosphoSitePlusi | Q96NN9 |
Genetic variation databases
BioMutai | AIFM3 |
DMDMi | 74732608 |
Proteomic databases
MassIVEi | Q96NN9 |
PaxDbi | Q96NN9 |
PeptideAtlasi | Q96NN9 |
PRIDEi | Q96NN9 |
ProteomicsDBi | 77540 [Q96NN9-1] 77541 [Q96NN9-2] 77542 [Q96NN9-3] |
Protocols and materials databases
Antibodypediai | 231, 200 antibodies |
DNASUi | 150209 |
Genome annotation databases
Ensembli | ENST00000399163; ENSP00000382116; ENSG00000183773 [Q96NN9-3] ENST00000399167; ENSP00000382120; ENSG00000183773 [Q96NN9-1] ENST00000405089; ENSP00000385800; ENSG00000183773 [Q96NN9-2] ENST00000440238; ENSP00000390798; ENSG00000183773 [Q96NN9-1] |
GeneIDi | 150209 |
KEGGi | hsa:150209 |
UCSCi | uc002ztj.3, human [Q96NN9-1] |
Organism-specific databases
CTDi | 150209 |
DisGeNETi | 150209 |
GeneCardsi | AIFM3 |
HGNCi | HGNC:26398, AIFM3 |
HPAi | ENSG00000183773, Group enriched (brain, intestine) |
MIMi | 617298, gene |
neXtProti | NX_Q96NN9 |
OpenTargetsi | ENSG00000183773 |
PharmGKBi | PA162376173 |
VEuPathDBi | HostDB:ENSG00000183773.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1336, Eukaryota |
GeneTreei | ENSGT00940000160448 |
InParanoidi | Q96NN9 |
OMAi | GAQLRYC |
OrthoDBi | 405030at2759 |
PhylomeDBi | Q96NN9 |
TreeFami | TF314028 |
Enzyme and pathway databases
PathwayCommonsi | Q96NN9 |
Miscellaneous databases
BioGRID-ORCSi | 150209, 14 hits in 876 CRISPR screens |
ChiTaRSi | AIFM3, human |
GenomeRNAii | 150209 |
Pharosi | Q96NN9, Tbio |
PROi | PR:Q96NN9 |
RNActi | Q96NN9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000183773, Expressed in mucosa of transverse colon and 135 other tissues |
ExpressionAtlasi | Q96NN9, baseline and differential |
Genevisiblei | Q96NN9, HS |
Family and domain databases
Gene3Di | 2.102.10.10, 1 hit 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR028202, Reductase_C IPR017941, Rieske_2Fe-2S IPR036922, Rieske_2Fe-2S_sf |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit PF14759, Reductase_C, 1 hit PF00355, Rieske, 1 hit |
SUPFAMi | SSF50022, SSF50022, 1 hit SSF51905, SSF51905, 1 hit SSF55424, SSF55424, 1 hit |
PROSITEi | View protein in PROSITE PS51296, RIESKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AIFM3_HUMAN | |
Accessioni | Q96NN9Primary (citable) accession number: Q96NN9 Secondary accession number(s): B7WP37 Q8N5E0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants