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Protein

Apoptosis-inducing factor 3

Gene

AIFM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi111Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi128Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi131Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Binding sitei235FADSequence analysis1
Binding sitei240FADSequence analysis1
Binding sitei270FAD; via amide nitrogen and carbonyl oxygenSequence analysis1
Binding sitei467FADSequence analysis1
Binding sitei514FAD; via carbonyl oxygenSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi201 – 205FADSequence analysis5

GO - Molecular functioni

GO - Biological processi

  • execution phase of apoptosis Source: BHF-UCL

Keywordsi

Molecular functionOxidoreductase
Biological processApoptosis, Electron transport, Transport
Ligand2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis-inducing factor 3 (EC:1.-.-.-)
Alternative name(s):
Apoptosis-inducing factor-like protein
Gene namesi
Name:AIFM3
Synonyms:AIFL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000183773.15
HGNCiHGNC:26398 AIFM3
MIMi617298 gene
neXtProtiNX_Q96NN9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi150209
OpenTargetsiENSG00000183773
PharmGKBiPA162376173

Polymorphism and mutation databases

BioMutaiAIFM3
DMDMi74732608

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002556601 – 605Apoptosis-inducing factor 3Add BLAST605

Proteomic databases

PaxDbiQ96NN9
PeptideAtlasiQ96NN9
PRIDEiQ96NN9
ProteomicsDBi77540
77541 [Q96NN9-2]
77542 [Q96NN9-3]

PTM databases

iPTMnetiQ96NN9
PhosphoSitePlusiQ96NN9

Expressioni

Tissue specificityi

Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000183773 Expressed in 113 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_AIFM3
ExpressionAtlasiQ96NN9 baseline and differential
GenevisibleiQ96NN9 HS

Organism-specific databases

HPAiHPA001271

Interactioni

Protein-protein interaction databases

BioGridi127270, 9 interactors
IntActiQ96NN9, 1 interactor
STRINGi9606.ENSP00000382120

Structurei

3D structure databases

ProteinModelPortaliQ96NN9
SMRiQ96NN9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini70 – 165RieskePROSITE-ProRule annotationAdd BLAST96

Domaini

The Rieske domain induces apoptosis.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1336 Eukaryota
COG0446 LUCA
COG2146 LUCA
GeneTreeiENSGT00530000063416
HOVERGENiHBG052926
InParanoidiQ96NN9
KOiK22747
OMAiGAQLRYC
OrthoDBiEOG091G07EG
PhylomeDBiQ96NN9
TreeFamiTF314028

Family and domain databases

Gene3Di2.102.10.10, 1 hit
3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR000103 Pyridine_nuc-diS_OxRdtase_2
IPR028202 Reductase_C
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF14759 Reductase_C, 1 hit
PF00355 Rieske, 1 hit
PRINTSiPR00469 PNDRDTASEII
SUPFAMiSSF50022 SSF50022, 1 hit
SSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit
PROSITEiView protein in PROSITE
PS51296 RIESKE, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q96NN9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGGCFSKPKP VELKIEVVLP EKERGKEELS ASGKGSPRAY QGNGTARHFH
60 70 80 90 100
TEERLSTPHP YPSPQDCVEA AVCHVKDLEN GQMREVELGW GKVLLVKDNG
110 120 130 140 150
EFHALGHKCP HYGAPLVKGV LSRGRVRCPW HGACFNISTG DLEDFPGLDS
160 170 180 190 200
LHKFQVKIEK EKVYVRASKQ ALQLQRRTKV MAKCISPSAG YSSSTNVLIV
210 220 230 240 250
GAGAAGLVCA ETLRQEGFSD RIVLCTLDRH LPYDRPKLSK SLDTQPEQLA
260 270 280 290 300
LRPKEFFRAY GIEVLTEAQV VTVDVRTKKV VFKDGFKLEY SKLLLAPGSS
310 320 330 340 350
PKTLSCKGKE VENVFTIRTP EDANRVVRLA RGRNVVVVGA GFLGMEVAAY
360 370 380 390 400
LTEKAHSVSV VELEETPFRR FLGERVGRAL MKMFENNRVK FYMQTEVSEL
410 420 430 440 450
RGQEGKLKEV VLKSSKVVRA DVCVVGIGAV PATGFLRQSG IGLDSRGFIP
460 470 480 490 500
VNKMMQTNVP GVFAAGDAVT FPLAWRNNRK VNIPHWQMAH AQGRVAAQNM
510 520 530 540 550
LAQEAEMSTV PYLWTAMFGK SLRYAGYGEG FDDVIIQGDL EELKFVAFYT
560 570 580 590 600
KGDEVIAVAS MNYDPIVSKV AEVLASGRAI RKREVELFVL HSKTGDMSWL

TGKGS
Length:605
Mass (Da):66,791
Last modified:December 1, 2001 - v1
Checksum:iC1DF20AE2AF2E262
GO
Isoform 2 (identifier: Q96NN9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: P → PGAALPT
     587-593: Missing.

Show »
Length:604
Mass (Da):66,477
Checksum:iAEF75FBE911988A6
GO
Isoform 3 (identifier: Q96NN9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     587-593: Missing.

Note: No experimental confirmation available.
Show »
Length:598
Mass (Da):65,966
Checksum:iE49D7ED5BBF7F30E
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9K029C9K029_HUMAN
Apoptosis-inducing factor 3
AIFM3
269Annotation score:
C9JPU8C9JPU8_HUMAN
Apoptosis-inducing factor 3
AIFM3
129Annotation score:
F8WFB6F8WFB6_HUMAN
Apoptosis-inducing factor 3
AIFM3
121Annotation score:
F8WC30F8WC30_HUMAN
Apoptosis-inducing factor 3
AIFM3
74Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165V → A in BAC04434 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061553508S → T. Corresponds to variant dbSNP:rs61356271Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02130010P → PGAALPT in isoform 2. 1 Publication1
Alternative sequenceiVSP_021303587 – 593Missing in isoform 2 and isoform 3. 2 Publications7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR456342 mRNA Translation: CAG30228.1
AK055035 mRNA Translation: BAB70841.1
AK094844 mRNA Translation: BAC04434.1
AC002470 Genomic DNA No translation available.
CH471176 Genomic DNA Translation: EAX02924.1
CH471176 Genomic DNA Translation: EAX02925.1
CH471176 Genomic DNA Translation: EAX02926.1
CH471176 Genomic DNA Translation: EAX02927.1
CH471176 Genomic DNA Translation: EAX02930.1
CH471176 Genomic DNA Translation: EAX02931.1
BC032485 mRNA Translation: AAH32485.1
CCDSiCCDS13786.1 [Q96NN9-1]
CCDS33605.1 [Q96NN9-3]
CCDS54503.1 [Q96NN9-2]
RefSeqiNP_001018070.1, NM_001018060.2 [Q96NN9-3]
NP_001139760.1, NM_001146288.1 [Q96NN9-2]
NP_653305.1, NM_144704.2 [Q96NN9-1]
UniGeneiHs.723246

Genome annotation databases

EnsembliENST00000399163; ENSP00000382116; ENSG00000183773 [Q96NN9-3]
ENST00000399167; ENSP00000382120; ENSG00000183773 [Q96NN9-1]
ENST00000405089; ENSP00000385800; ENSG00000183773 [Q96NN9-2]
ENST00000440238; ENSP00000390798; ENSG00000183773 [Q96NN9-1]
GeneIDi150209
KEGGihsa:150209
UCSCiuc002ztj.3 human [Q96NN9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAIFM3_HUMAN
AccessioniPrimary (citable) accession number: Q96NN9
Secondary accession number(s): B7WP37
, D3DX37, D3DX38, Q6ZT44, Q8N1V3, Q8N5E0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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