SPATA13

Functionⁱ

Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.5 Publications

Activity regulationⁱ

Both the ABR and the SH3 domains contribute to maintaining the protein in an inhibited conformation by associating with the C-terminal tail. Binding of these domains to the C-terminal tail inhibits the activity of the protein by blocking a region that is required for its GEF activity.1 Publication

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Guanine-nucleotide releasing factor

Molecular function

Guanine-nucleotide releasing factor

Enzyme and pathway databases

PathwayCommonsⁱ	Q96N96
SIGNORⁱ	Q96N96

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Spermatogenesis-associated protein 13 Alternative name(s): APC-stimulated guanine nucleotide exchange factor 2 Short name: Asef2
Gene namesⁱ	Name:SPATA131 PublicationImported
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 13

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000182957.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:23222, SPATA13
MIMⁱ	613324, gene
neXtProtⁱ	NX_Q96N96

Keywords - Cellular componentⁱ

Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	221178
Open Targets More...OpenTargetsⁱ	ENSG00000182957
PharmGKBⁱ	PA134912609

Miscellaneous databases

Pharosⁱ	Q96N96, Tbio

Pharosⁱ

Q96N96, Tbio

Polymorphism and mutation databases

BioMutaⁱ	SPATA13
DMDMⁱ	74752049

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000278448	1 – 652	Spermatogenesis-associated protein 13Add BLAST		652

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	78	PhosphoserineBy similarity		1
Modified residueⁱ	114	PhosphoserineBy similarity		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

jPOSTⁱ	Q96N96
MassIVEⁱ	Q96N96
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q96N96
PaxDbⁱ	Q96N96
PeptideAtlas More...PeptideAtlasⁱ	Q96N96
PRIDEⁱ	Q96N96
ProteomicsDBⁱ	77489 [Q96N96-1] 77490 [Q96N96-2] 77491 [Q96N96-3] 77492 [Q96N96-4] 77493 [Q96N96-5]

PTM databases

iPTMnetⁱ	Q96N96
PhosphoSitePlusⁱ	Q96N96

Expressionⁱ

Tissue specificityⁱ

Expressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors.3 Publications

Gene expression databases

Bgeeⁱ	ENSG00000182957, Expressed in metanephros and 214 other tissues
ExpressionAtlasⁱ	Q96N96, baseline and differential
Genevisibleⁱ	Q96N96, HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	ENSG00000182957, Low tissue specificity

HPAⁱ

ENSG00000182957, Low tissue specificity

Interactionⁱ

Subunit structureⁱ

Interacts (via ABR and SH3 domain) with APC. The binding of APC enhances its GEF activity by relieving it from an autoinhibitory conformation, in which the ABR and SH3 domains are associated with the C-terminal tail.

Interacts (via C-terminal tail) with PPP1R9B (via C-terminus).

Interacts with RAC1.

3 Publications

Binary interactionsⁱ

Hide details

Q96N96

With	#Exp.	IntAct
APC [P25054]	5	EBI-13618641,EBI-727707

SPATA13 - isoform 1 [Q96N96-1]

With	#Exp.	IntAct
APC [P25054]	5	EBI-13638906,EBI-727707
itself	4	EBI-13638906,EBI-13638906

Isoform 2 [Q96N96-2]

With	#Exp.	IntAct
APC [P25054]	2	EBI-13639118,EBI-727707

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGRIDⁱ	128693, 12 interactors
IntActⁱ	Q96N96, 12 interactors
STRINGⁱ	9606.ENSP00000398560

Miscellaneous databases

RNActⁱ	Q96N96, protein

RNActⁱ

Q96N96, protein

Structureⁱ

3D structure databases

SMRⁱ	Q96N96
ModBaseⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	147 – 206	SH3PROSITE-ProRule annotationAdd BLAST	60
Domainⁱ	240 – 424	DHPROSITE-ProRule annotationAdd BLAST	185
Domainⁱ	455 – 561	PHPROSITE-ProRule annotationAdd BLAST	107

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	98 – 150	ABR (APC-binding region) domainAdd BLAST		53
Regionⁱ	561 – 652	C-terminal tailAdd BLAST		92

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	20 – 23	Poly-Arg		4

Domainⁱ

The C-terminal tail is required for its GEF activity.

Keywords - Domainⁱ

SH3 domain

Phylogenomic databases

eggNOGⁱ	KOG3519, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00940000154103
HOGENOMⁱ	CLU_008436_0_0_1
InParanoidⁱ	Q96N96
OMAⁱ	KAQSHHN
Database of Orthologous Groups More...OrthoDBⁱ	65769at2759
PhylomeDBⁱ	Q96N96
TreeFamⁱ	TF316832

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00160, RhoGEF, 1 hit
Gene3Dⁱ	1.20.900.10, 1 hit 2.30.29.30, 1 hit
InterProⁱ	View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00018, SH3_1, 1 hit
SMARTⁱ	View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00326, SH3, 1 hit
SUPFAMⁱ	SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit
PROSITEⁱ	View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit

Sequences (6+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 6 isoformsⁱ produced by alternative splicing. Align Add to basket

Note: Additional isoforms seem to exist.

This entry has 6 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q96N96-1) [UniParc]FASTA Add to basket

Also known as: ASEF-2b

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MTSASPEDQN APVGCPKGAR RRRPISVIGG VSLYGTNQTE ELDNLLTQPA 
        60         70         80         90        100
SRPPMPAHQV PPYKAVSARF RPFTFSQSTP IGLDRVGRRR QMRASNVSSD 
       110        120        130        140        150
GGTEPSALVD DNGSEEDFSY EDLCQASPRY LQPGGEQLAI NELISDGNVV 
       160        170        180        190        200
CAEALWDHVT MDDQELGFKA GDVIQVLEAS NKDWWWGRSE DKEAWFPASF 
       210        220        230        240        250
VRLRVNQEEL SENSSSTPSE EQDEEASQSR HRHCENKQQM RTNVIREIMD 
       260        270        280        290        300
TERVYIKHLR DICEGYIRQC RKHTGMFTVA QLATIFGNIE DIYKFQRKFL 
       310        320        330        340        350
KDLEKQYNKE EPHLSEIGSC FLQNQEGFAI YSEYCNNHPG ACLELANLMK 
       360        370        380        390        400
QGKYRHFFEA CRLLQQMIDI AIDGFLLTPV QKICKYPLQL AELLKYTTQE 
       410        420        430        440        450
HGDYSNIKAA YEAMKNVACL INERKRKLES IDKIARWQVS IVGWEGLDIL 
       460        470        480        490        500
DRSSELIHSG ELTKITKQGK SQQRTFFLFD HQLVSCKKDL LRRDMLYYKG 
       510        520        530        540        550
RLDMDEMELV DLGDGRDKDC NLSVKNAFKL VSRTTDEVYL FCAKKQEDKA 
       560        570        580        590        600
RWLQACADER RRVQEDKEMG MEISENQKKL AMLNAQKAGH GKSKGYNRCP 
       610        620        630        640        650
VAPPHQGLHP IHQRHITMPT SVPQQQVFGL AEPKRKSSLF WHTFNRLTPF 

RK

Length:652

Mass (Da):74,820

Last modified:December 1, 2001 - v1

Checksum:ⁱ7C922E8AF0A9C836

GO

Isoform 2 (identifier: Q96N96-2) [UniParc]FASTA Add to basket

Also known as: ASEF-2a

The sequence of this isoform differs from the canonical sequence as follows:
1-96: MTSASPEDQN...GRRRQMRASN → MVARGEIARFWSLESLHL

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        10         20         30         40         50
MVARGEIARF WSLESLHLVS SDGGTEPSAL VDDNGSEEDF SYEDLCQASP 
        60         70         80         90        100
RYLQPGGEQL AINELISDGN VVCAEALWDH VTMDDQELGF KAGDVIQVLE 
       110        120        130        140        150
ASNKDWWWGR SEDKEAWFPA SFVRLRVNQE ELSENSSSTP SEEQDEEASQ 
       160        170        180        190        200
SRHRHCENKQ QMRTNVIREI MDTERVYIKH LRDICEGYIR QCRKHTGMFT 
       210        220        230        240        250
VAQLATIFGN IEDIYKFQRK FLKDLEKQYN KEEPHLSEIG SCFLQNQEGF 
       260        270        280        290        300
AIYSEYCNNH PGACLELANL MKQGKYRHFF EACRLLQQMI DIAIDGFLLT 
       310        320        330        340        350
PVQKICKYPL QLAELLKYTT QEHGDYSNIK AAYEAMKNVA CLINERKRKL 
       360        370        380        390        400
ESIDKIARWQ VSIVGWEGLD ILDRSSELIH SGELTKITKQ GKSQQRTFFL 
       410        420        430        440        450
FDHQLVSCKK DLLRRDMLYY KGRLDMDEME LVDLGDGRDK DCNLSVKNAF 
       460        470        480        490        500
KLVSRTTDEV YLFCAKKQED KARWLQACAD ERRRVQEDKE MGMEISENQK 
       510        520        530        540        550
KLAMLNAQKA GHGKSKGYNR CPVAPPHQGL HPIHQRHITM PTSVPQQQVF 
       560        570 
GLAEPKRKSS LFWHTFNRLT PFRK

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Length:574

Mass (Da):66,403

Checksum:ⁱ34A1B7ECAF3C4C30

GO

Isoform 3 (identifier: Q96N96-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-96: MTSASPEDQN...GRRRQMRASN → MGFIIHIQQV...EKKCASLSFE

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        10         20         30         40         50
MGFIIHIQQV KKQRKKLDQG LILKKEKYRK EKKCASLSFE VSSDGGTEPS 
        60         70         80         90        100
ALVDDNGSEE DFSYEDLCQA SPRYLQPGGE QLAINELISD GNVVCAEALW 
       110        120        130        140        150
DHVTMDDQEL GFKAGDVIQV LEASNKDWWW GRSEDKEAWF PASFVRLRVN 
       160        170        180        190        200
QEELSENSSS TPSEEQDEEA SQSRHRHCEN KQQMRTNVIR EIMDTERVYI 
       210        220        230        240        250
KHLRDICEGY IRQCRKHTGM FTVAQLATIF GNIEDIYKFQ RKFLKDLEKQ 
       260        270        280        290        300
YNKEEPHLSE IGSCFLQNQE GFAIYSEYCN NHPGACLELA NLMKQGKYRH 
       310        320        330        340        350
FFEACRLLQQ MIDIAIDGFL LTPVQKICKY PLQLAELLKY TTQEHGDYSN 
       360        370        380        390        400
IKAAYEAMKN VACLINERKR KLESIDKIAR WQVSIVGWEG LDILDRSSEL 
       410        420        430        440        450
IHSGELTKIT KQGKSQQRTF FLFDHQLVSC KKDLLRRDML YYKGRLDMDE 
       460        470        480        490        500
MELVDLGDGR DKDCNLSVKN AFKLVSRTTD EVYLFCAKKQ EDKARWLQAC 
       510        520        530        540        550
ADERRRVQED KEMGMEISEN QKKLAMLNAQ KAGHGKSKGY NRCPVAPPHQ 
       560        570        580        590 
GLHPIHQRHI TMPTSVPQQQ VFGLAEPKRK SSLFWHTFNR LTPFRK

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Length:596

Mass (Da):69,108

Checksum:ⁱCBD88B1A30AF9B9A

GO

Isoform 4 (identifier: Q96N96-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-54: Missing.
595-652: GYNRCPVAPP...TFNRLTPFRK → VRLQQVPRGP...SMELGVKRRN

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        10         20         30         40         50
MPAHQVPPYK AVSARFRPFT FSQSTPIGLD RVGRRRQMRA SNVSSDGGTE 
        60         70         80         90        100
PSALVDDNGS EEDFSYEDLC QASPRYLQPG GEQLAINELI SDGNVVCAEA 
       110        120        130        140        150
LWDHVTMDDQ ELGFKAGDVI QVLEASNKDW WWGRSEDKEA WFPASFVRLR 
       160        170        180        190        200
VNQEELSENS SSTPSEEQDE EASQSRHRHC ENKQQMRTNV IREIMDTERV 
       210        220        230        240        250
YIKHLRDICE GYIRQCRKHT GMFTVAQLAT IFGNIEDIYK FQRKFLKDLE 
       260        270        280        290        300
KQYNKEEPHL SEIGSCFLQN QEGFAIYSEY CNNHPGACLE LANLMKQGKY 
       310        320        330        340        350
RHFFEACRLL QQMIDIAIDG FLLTPVQKIC KYPLQLAELL KYTTQEHGDY 
       360        370        380        390        400
SNIKAAYEAM KNVACLINER KRKLESIDKI ARWQVSIVGW EGLDILDRSS 
       410        420        430        440        450
ELIHSGELTK ITKQGKSQQR TFFLFDHQLV SCKKDLLRRD MLYYKGRLDM 
       460        470        480        490        500
DEMELVDLGD GRDKDCNLSV KNAFKLVSRT TDEVYLFCAK KQEDKARWLQ 
       510        520        530        540        550
ACADERRRVQ EDKEMGMEIS ENQKKLAMLN AQKAGHGKSK VRLQQVPRGP 
       560        570        580        590        600
TAPGPAPHPP APHHYAHKRP PAAGLWPGGT QEEVLALLAH LQQAHPLPEM 
       610 
KTGGCASMEL GVKRRN

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Length:616

Mass (Da):70,446

Checksum:ⁱ15F635D80BA6B3C7

GO

Isoform 5 (identifier: Q96N96-5) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-54: Missing.
203-264: Missing.

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        10         20         30         40         50
MPAHQVPPYK AVSARFRPFT FSQSTPIGLD RVGRRRQMRA SNVSSDGGTE 
        60         70         80         90        100
PSALVDDNGS EEDFSYEDLC QASPRYLQPG GEQLAINELI SDGNVVCAEA 
       110        120        130        140        150
LWDHVTMDDQ ELGFKAGDVI QVLEASNKDW WWGRSEDKEA WFPASFVRGY 
       160        170        180        190        200
IRQCRKHTGM FTVAQLATIF GNIEDIYKFQ RKFLKDLEKQ YNKEEPHLSE 
       210        220        230        240        250
IGSCFLQNQE GFAIYSEYCN NHPGACLELA NLMKQGKYRH FFEACRLLQQ 
       260        270        280        290        300
MIDIAIDGFL LTPVQKICKY PLQLAELLKY TTQEHGDYSN IKAAYEAMKN 
       310        320        330        340        350
VACLINERKR KLESIDKIAR WQVSIVGWEG LDILDRSSEL IHSGELTKIT 
       360        370        380        390        400
KQGKSQQRTF FLFDHQLVSC KKDLLRRDML YYKGRLDMDE MELVDLGDGR 
       410        420        430        440        450
DKDCNLSVKN AFKLVSRTTD EVYLFCAKKQ EDKARWLQAC ADERRRVQED 
       460        470        480        490        500
KEMGMEISEN QKKLAMLNAQ KAGHGKSKGY NRCPVAPPHQ GLHPIHQRHI 
       510        520        530 
TMPTSVPQQQ VFGLAEPKRK SSLFWHTFNR LTPFRK

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Length:536

Mass (Da):61,677

Checksum:ⁱF449FEF56512EA27

GO

Isoform 6 (identifier: Q96N96-6) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-1: M → MTQAAVRPWA...RVDEDPQASM

« Hide

        10         20         30         40         50
MTQAAVRPWA PCLENMTTAP NGLGPGPAAP CAGSDLKDAK MVTSLACGNG 
        60         70         80         90        100
VCGCSPGGDT DTQEAKLSPA KLVRLFSTSR KRTGAHPERP HSMVLVGNSS 
       110        120        130        140        150
TWNTLASFRK MGSFKKLKSS VLKGIQSREG SNACSKGEAS EHGLGKSIPN 
       160        170        180        190        200
GAVPGAQASR GSPLAPGPAC GALRPAEWGT LDGSDLEDTD DAFQRSTHRS 
       210        220        230        240        250
RSLRRAYGLG RICLLDAPQN HATPTIATGQ VPAVCEILVR DPENNSMGYR 
       260        270        280        290        300
RSKSTDNLAF LKKSSFKRKS TSNLADLRTA HDARVPQRTL SSSSTDSQKL 
       310        320        330        340        350
GSGRTKRWRS PIRAKDFDRV FKLVSNVTEA AWRRESPRSG APSPGEASLR 
       360        370        380        390        400
LQAHSRLHDD YSRRVSRSTE QDSRRGGAVM HGTTATCTVA PGFGSATSKG 
       410        420        430        440        450
PHLDADTAVF PLETKSSWAV ESDSSCTCSS LPSPIVQDVL SKDSCDPNAG 
       460        470        480        490        500
SQLTFDPEQP PTPLRPTTPK PQSPQSPQSP GAGSASCHSN HSALSANSEE 
       510        520        530        540        550
SEGRAEEPAQ REPGPVSLQD PLEATHGDEG SKDLLVNIGV AAGPEEKEKE 
       560        570        580        590        600
EVVPDGPWRR SSSQDEERTE AQRTPKRRWG SGRRPRPRPF SDYGQLASRS 
       610        620        630        640        650
LSIPEDSVAA DPQKEDRVDE DPQASMTSAS PEDQNAPVGC PKGARRRRPI 
       660        670        680        690        700
SVIGGVSLYG TNQTEELDNL LTQPASRPPM PAHQVPPYKA VSARFRPFTF 
       710        720        730        740        750
SQSTPIGLDR VGRRRQMRAS NVSSDGGTEP SALVDDNGSE EDFSYEDLCQ 
       760        770        780        790        800
ASPRYLQPGG EQLAINELIS DGNVVCAEAL WDHVTMDDQE LGFKAGDVIQ 
       810        820        830        840        850
VLEASNKDWW WGRSEDKEAW FPASFVRLRV NQEELSENSS STPSEEQDEE 
       860        870        880        890        900
ASQSRHRHCE NKQQMRTNVI REIMDTERVY IKHLRDICEG YIRQCRKHTG 
       910        920        930        940        950
MFTVAQLATI FGNIEDIYKF QRKFLKDLEK QYNKEEPHLS EIGSCFLQNQ 
       960        970        980        990       1000
EGFAIYSEYC NNHPGACLEL ANLMKQGKYR HFFEACRLLQ QMIDIAIDGF 
      1010       1020       1030       1040       1050
LLTPVQKICK YPLQLAELLK YTTQEHGDYS NIKAAYEAMK NVACLINERK 
      1060       1070       1080       1090       1100
RKLESIDKIA RWQVSIVGWE GLDILDRSSE LIHSGELTKI TKQGKSQQRT 
      1110       1120       1130       1140       1150
FFLFDHQLVS CKKDLLRRDM LYYKGRLDMD EMELVDLGDG RDKDCNLSVK 
      1160       1170       1180       1190       1200
NAFKLVSRTT DEVYLFCAKK QEDKARWLQA CADERRRVQE DKEMGMEISE 
      1210       1220       1230       1240       1250
NQKKLAMLNA QKAGHGKSKG YNRCPVAPPH QGLHPIHQRH ITMPTSVPQQ 
      1260       1270 
QVFGLAEPKR KSSLFWHTFN RLTPFRK

Show »

Length:1,277

Mass (Da):141,436

Checksum:ⁱ23BC07A9333FB2C7

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

J3KQJ8	J3KQJ8_HUMAN	Spermatogenesis-associated protein ... Spermatogenesis-associated protein 13	SPATA13	550	Annotation score:
E9PFR9	E9PFR9_HUMAN	Spermatogenesis-associated protein ... Spermatogenesis-associated protein 13	SPATA13	512	Annotation score:
Q5VX67	Q5VX67_HUMAN	Spermatogenesis-associated protein ... Spermatogenesis-associated protein 13	SPATA13	146	Annotation score:
V9GZ16	V9GZ16_HUMAN	Spermatogenesis-associated protein ... Spermatogenesis-associated protein 13	SPATA13	23	Annotation score:

Sequence cautionⁱ

The sequence BAC04977 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	304	E → G in BAG61802 (PubMed:14702039).Curated	1
Sequence conflictⁱ	520	C → R in BAG62740 (PubMed:14702039).Curated	1
Sequence conflictⁱ	537	E → G in BAG62740 (PubMed:14702039).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_030776	20	R → W. Corresponds to variant dbSNP:rs7330736Ensembl.		1
Natural variantⁱVAR_080767	89	R → W Found in two consanguineous families with intellectual disability; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs201402934Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_041030	1 – 96	MTSAS…MRASN → MVARGEIARFWSLESLHL in isoform 2. 1 PublicationAdd BLAST	96
Alternative sequenceⁱVSP_041031	1 – 96	MTSAS…MRASN → MGFIIHIQQVKKQRKKLDQG LILKKEKYRKEKKCASLSFE in isoform 3. 1 PublicationAdd BLAST	96
Alternative sequenceⁱVSP_041032	1 – 54	Missing in isoform 4 and isoform 5. 1 PublicationAdd BLAST	54
Alternative sequenceⁱVSP_054112	1	M → MTQAAVRPWAPCLENMTTAP NGLGPGPAAPCAGSDLKDAK MVTSLACGNGVCGCSPGGDT DTQEAKLSPAKLVRLFSTSR KRTGAHPERPHSMVLVGNSS TWNTLASFRKMGSFKKLKSS VLKGIQSREGSNACSKGEAS EHGLGKSIPNGAVPGAQASR GSPLAPGPACGALRPAEWGT LDGSDLEDTDDAFQRSTHRS RSLRRAYGLGRICLLDAPQN HATPTIATGQVPAVCEILVR DPENNSMGYRRSKSTDNLAF LKKSSFKRKSTSNLADLRTA HDARVPQRTLSSSSTDSQKL GSGRTKRWRSPIRAKDFDRV FKLVSNVTEAAWRRESPRSG APSPGEASLRLQAHSRLHDD YSRRVSRSTEQDSRRGGAVM HGTTATCTVAPGFGSATSKG PHLDADTAVFPLETKSSWAV ESDSSCTCSSLPSPIVQDVL SKDSCDPNAGSQLTFDPEQP PTPLRPTTPKPQSPQSPQSP GAGSASCHSNHSALSANSEE SEGRAEEPAQREPGPVSLQD PLEATHGDEGSKDLLVNIGV AAGPEEKEKEEVVPDGPWRR SSSQDEERTEAQRTPKRRWG SGRRPRPRPFSDYGQLASRS LSIPEDSVAADPQKEDRVDE DPQASM in isoform 6. Curated	1
Alternative sequenceⁱVSP_041033	203 – 264	Missing in isoform 5. 1 PublicationAdd BLAST	62
Alternative sequenceⁱVSP_041034	595 – 652	GYNRC…TPFRK → VRLQQVPRGPTAPGPAPHPP APHHYAHKRPPAAGLWPGGT QEEVLALLAHLQQAHPLPEM KTGGCASMELGVKRRN in isoform 4. 1 PublicationAdd BLAST	58

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BK006072 mRNA Translation: DAA05848.1 AK055770 mRNA Translation: BAB71009.1 AK097217 mRNA Translation: BAC04977.1 Different initiation. AK123031 mRNA Translation: BAG53856.1 AK298717 mRNA Translation: BAG60873.1 AK299981 mRNA Translation: BAG61802.1 AK301149 mRNA Translation: BAG62740.1 AL136963 Genomic DNA No translation available. AL139324 Genomic DNA No translation available. AL359736 Genomic DNA No translation available. AL445985 Genomic DNA No translation available. CH471075 Genomic DNA Translation: EAX08331.1 BC109290 mRNA Translation: AAI09291.1 BC109291 mRNA Translation: AAI09292.1 AK074117 mRNA Translation: BAB84943.1 AK160371 mRNA Translation: BAD18714.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS53857.1 [Q96N96-6] CCDS66517.1 [Q96N96-2] CCDS66518.1 [Q96N96-3] CCDS9305.1 [Q96N96-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001159743.1, NM_001166271.2 [Q96N96-6] NP_001273721.1, NM_001286792.1 NP_001273722.1, NM_001286793.1 NP_001273723.1, NM_001286794.1 [Q96N96-3] NP_001273724.1, NM_001286795.1 [Q96N96-2] NP_694568.1, NM_153023.3 [Q96N96-1]

Genome annotation databases

Ensemblⁱ	ENST00000343003; ENSP00000343631; ENSG00000182957 [Q96N96-3] ENST00000382095; ENSP00000371527; ENSG00000182957 [Q96N96-1] ENST00000382108; ENSP00000371542; ENSG00000182957 [Q96N96-6] ENST00000399949; ENSP00000382830; ENSG00000182957 [Q96N96-2] ENST00000424834; ENSP00000398560; ENSG00000182957 [Q96N96-6]
GeneIDⁱ	221178
KEGGⁱ	hsa:221178
UCSC genome browser More...UCSCⁱ	uc001upg.4, human [Q96N96-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q96N96	Isoform 6 of Spermatogenesis-associated protein 13		HUMAN		1277	UniRef100_Q96N96
SPATA13		HUMAN		652
+1
Q96N96-3	Spermatogenesis-associated protein 13 (Fragment)		HUMAN		23	UniRef100_Q96N96-3
Spermatogenesis-associated protein 13 (Fragment)		HUMAN		146
Q96N96-5	Spermatogenesis-associated protein 13 (Fragment)		HUMAN		550	UniRef100_Q96N96-5

Protein	Similar proteins		Species	Score	Length	Source
Q96N96	Isoform 6 of Spermatogenesis-associated protein 13		HUMAN		1277	UniRef90_Q96N96
Uncharacterized protein		RHIBE		1325
Uncharacterized protein		PANPA		1279
Spermatogenesis associated 13		PANTR		1279
Uncharacterized protein		RHIRO		1274
+25
Q96N96-2	Isoform 3 of Spermatogenesis-associated protein 13		HUMAN		596	UniRef90_Q96N96-3
Uncharacterized protein		MACNE		597
Uncharacterized protein		PAPAN		597
Uncharacterized protein		COLAP		596
SPATA13 isoform 2		PONAB		596
+35
Q96N96-5	Spermatogenesis-associated protein 13 (Fragment)		HUMAN		550	UniRef90_Q96N96-5
SPATA13 isoform 7 (Fragment)		PONAB		550
SPATA13 isoform 6		PONAB		512

Protein	Similar proteins		Species	Score	Length	Source
Q96N96	Spermatogenesis-associated protein 13		MOUSE		656	UniRef50_Q96N96
Isoform 6 of Spermatogenesis-associated protein 13		HUMAN		1277
Uncharacterized protein		RHIBE		1325
Spermatogenesis associated 13		PANTR		1279
Uncharacterized protein		PANPA		1279
+219
Q96N96-2	Isoform 3 of Spermatogenesis-associated protein 13		HUMAN		596	UniRef50_Q96N96-3
Uncharacterized protein		MACNE		597
Uncharacterized protein		PAPAN		597
SPATA13 isoform 2		PONAB		596
Uncharacterized protein		COLAP		596
+259
Q96N96-4	Rho guanine nucleotide exchange factor 4 (Fragment)		CALAN		592	UniRef50_Q96N96-4
Uncharacterized protein		PHACC		681
Uncharacterized protein (Fragment)		COLVI		562
rho guanine nucleotide exchange factor 4 isoform X6		ACIJB		677
Rho guanine nucleotide exchange factor 4		FELCA		678
+205

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BK006072 mRNA Translation: DAA05848.1 AK055770 mRNA Translation: BAB71009.1 AK097217 mRNA Translation: BAC04977.1 Different initiation. AK123031 mRNA Translation: BAG53856.1 AK298717 mRNA Translation: BAG60873.1 AK299981 mRNA Translation: BAG61802.1 AK301149 mRNA Translation: BAG62740.1 AL136963 Genomic DNA No translation available. AL139324 Genomic DNA No translation available. AL359736 Genomic DNA No translation available. AL445985 Genomic DNA No translation available. CH471075 Genomic DNA Translation: EAX08331.1 BC109290 mRNA Translation: AAI09291.1 BC109291 mRNA Translation: AAI09292.1 AK074117 mRNA Translation: BAB84943.1 AK160371 mRNA Translation: BAD18714.1
CCDSⁱ	CCDS53857.1 [Q96N96-6] CCDS66517.1 [Q96N96-2] CCDS66518.1 [Q96N96-3] CCDS9305.1 [Q96N96-1]
RefSeqⁱ	NP_001159743.1, NM_001166271.2 [Q96N96-6] NP_001273721.1, NM_001286792.1 NP_001273722.1, NM_001286793.1 NP_001273723.1, NM_001286794.1 [Q96N96-3] NP_001273724.1, NM_001286795.1 [Q96N96-2] NP_694568.1, NM_153023.3 [Q96N96-1]

3D structure databases

SMRⁱ	Q96N96
ModBaseⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	128693, 12 interactors
IntActⁱ	Q96N96, 12 interactors
STRINGⁱ	9606.ENSP00000398560

PTM databases

iPTMnetⁱ	Q96N96
PhosphoSitePlusⁱ	Q96N96

Polymorphism and mutation databases

BioMutaⁱ	SPATA13
DMDMⁱ	74752049

Proteomic databases

jPOSTⁱ	Q96N96
MassIVEⁱ	Q96N96
MaxQBⁱ	Q96N96
PaxDbⁱ	Q96N96
PeptideAtlasⁱ	Q96N96
PRIDEⁱ	Q96N96
ProteomicsDBⁱ	77489 [Q96N96-1] 77490 [Q96N96-2] 77491 [Q96N96-3] 77492 [Q96N96-4] 77493 [Q96N96-5]

Protocols and materials databases

Antibodypediaⁱ	49852, 89 antibodies

Antibodypediaⁱ

49852, 89 antibodies

Genome annotation databases

Ensemblⁱ	ENST00000343003; ENSP00000343631; ENSG00000182957 [Q96N96-3] ENST00000382095; ENSP00000371527; ENSG00000182957 [Q96N96-1] ENST00000382108; ENSP00000371542; ENSG00000182957 [Q96N96-6] ENST00000399949; ENSP00000382830; ENSG00000182957 [Q96N96-2] ENST00000424834; ENSP00000398560; ENSG00000182957 [Q96N96-6]
GeneIDⁱ	221178
KEGGⁱ	hsa:221178
UCSCⁱ	uc001upg.4, human [Q96N96-1]

Organism-specific databases

CTDⁱ	221178
DisGeNETⁱ	221178
EuPathDBⁱ	HostDB:ENSG00000182957.15
GeneCardsⁱ	SPATA13
HGNCⁱ	HGNC:23222, SPATA13
HPAⁱ	ENSG00000182957, Low tissue specificity
MIMⁱ	613324, gene
neXtProtⁱ	NX_Q96N96
OpenTargetsⁱ	ENSG00000182957
PharmGKBⁱ	PA134912609
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG3519, Eukaryota
GeneTreeⁱ	ENSGT00940000154103
HOGENOMⁱ	CLU_008436_0_0_1
InParanoidⁱ	Q96N96
OMAⁱ	KAQSHHN
OrthoDBⁱ	65769at2759
PhylomeDBⁱ	Q96N96
TreeFamⁱ	TF316832

Enzyme and pathway databases

PathwayCommonsⁱ	Q96N96
SIGNORⁱ	Q96N96

Miscellaneous databases

BioGRID-ORCSⁱ	221178, 10 hits in 846 CRISPR screens
ChiTaRSⁱ	SPATA13, human
GenomeRNAiⁱ	221178
Pharosⁱ	Q96N96, Tbio
Protein Ontology More...PROⁱ	PR:Q96N96
RNActⁱ	Q96N96, protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000182957, Expressed in metanephros and 214 other tissues
ExpressionAtlasⁱ	Q96N96, baseline and differential
Genevisibleⁱ	Q96N96, HS

Family and domain databases

CDDⁱ	cd00160, RhoGEF, 1 hit
Gene3Dⁱ	1.20.900.10, 1 hit 2.30.29.30, 1 hit
InterProⁱ	View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain
Pfamⁱ	View protein in Pfam PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00018, SH3_1, 1 hit
SMARTⁱ	View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00326, SH3, 1 hit
SUPFAMⁱ	SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit
PROSITEⁱ	View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SPT13_HUMAN
Accessionⁱ	Q96N96Primary (citable) accession number: Q96N96 Secondary accession number(s): A2VEA9 Q8TEK6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
	Last sequence update:	December 1, 2001
	Last modified:	December 2, 2020
	This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Reference proteome

Documents

Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 13
Human chromosome 13: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations

Annotation systems

Supporting data

UniProtKB - Q96N96 (SPT13_HUMAN)

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Spermatogenesis-associated protein 13

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Cytosol

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Miscellaneous databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ