UniProtKB - Q96N96 (SPT13_HUMAN)
Spermatogenesis-associated protein 13
SPATA13
Functioni
Activity regulationi
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: UniProtKB
- identical protein binding Source: IntAct
- Rac guanyl-nucleotide exchange factor activity Source: UniProtKB
GO - Biological processi
- cell migration Source: UniProtKB
- filopodium assembly Source: UniProtKB
- lamellipodium assembly Source: UniProtKB
- regulation of cell migration Source: UniProtKB
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Enzyme and pathway databases
PathwayCommonsi | Q96N96 |
SIGNORi | Q96N96 |
Names & Taxonomyi
Protein namesi | Recommended name: Spermatogenesis-associated protein 13Alternative name(s): APC-stimulated guanine nucleotide exchange factor 2 Short name: Asef2 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000182957.15 |
HGNCi | HGNC:23222, SPATA13 |
MIMi | 613324, gene |
neXtProti | NX_Q96N96 |
Subcellular locationi
Plasma membrane
Other locations
Note: Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- ruffle membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- filopodium Source: UniProtKB
- lamellipodium Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 221178 |
OpenTargetsi | ENSG00000182957 |
PharmGKBi | PA134912609 |
Miscellaneous databases
Pharosi | Q96N96, Tbio |
Polymorphism and mutation databases
BioMutai | SPATA13 |
DMDMi | 74752049 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000278448 | 1 – 652 | Spermatogenesis-associated protein 13Add BLAST | 652 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 78 | PhosphoserineBy similarity | 1 | |
Modified residuei | 114 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q96N96 |
MassIVEi | Q96N96 |
MaxQBi | Q96N96 |
PaxDbi | Q96N96 |
PeptideAtlasi | Q96N96 |
PRIDEi | Q96N96 |
ProteomicsDBi | 77489 [Q96N96-1] 77490 [Q96N96-2] 77491 [Q96N96-3] 77492 [Q96N96-4] 77493 [Q96N96-5] |
PTM databases
iPTMneti | Q96N96 |
PhosphoSitePlusi | Q96N96 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000182957, Expressed in metanephros and 214 other tissues |
ExpressionAtlasi | Q96N96, baseline and differential |
Genevisiblei | Q96N96, HS |
Organism-specific databases
HPAi | ENSG00000182957, Low tissue specificity |
Interactioni
Subunit structurei
Interacts (via ABR and SH3 domain) with APC. The binding of APC enhances its GEF activity by relieving it from an autoinhibitory conformation, in which the ABR and SH3 domains are associated with the C-terminal tail.
Interacts (via C-terminal tail) with PPP1R9B (via C-terminus).
Interacts with RAC1.
3 PublicationsBinary interactionsi
Hide detailsQ96N96
With | #Exp. | IntAct |
---|---|---|
APC [P25054] | 5 | EBI-13618641,EBI-727707 |
SPATA13 - isoform 1 [Q96N96-1]
With | #Exp. | IntAct |
---|---|---|
APC [P25054] | 5 | EBI-13638906,EBI-727707 |
itself | 4 | EBI-13638906,EBI-13638906 |
Isoform 2 [Q96N96-2]
With | #Exp. | IntAct |
---|---|---|
APC [P25054] | 2 | EBI-13639118,EBI-727707 |
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: UniProtKB
- identical protein binding Source: IntAct
- Rac guanyl-nucleotide exchange factor activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 128693, 12 interactors |
IntActi | Q96N96, 12 interactors |
STRINGi | 9606.ENSP00000398560 |
Miscellaneous databases
RNActi | Q96N96, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 147 – 206 | SH3PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 240 – 424 | DHPROSITE-ProRule annotationAdd BLAST | 185 | |
Domaini | 455 – 561 | PHPROSITE-ProRule annotationAdd BLAST | 107 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 98 – 150 | ABR (APC-binding region) domainAdd BLAST | 53 | |
Regioni | 561 – 652 | C-terminal tailAdd BLAST | 92 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 20 – 23 | Poly-Arg | 4 |
Domaini
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG3519, Eukaryota |
GeneTreei | ENSGT00940000154103 |
HOGENOMi | CLU_008436_0_0_1 |
InParanoidi | Q96N96 |
OMAi | KAQSHHN |
OrthoDBi | 65769at2759 |
PhylomeDBi | Q96N96 |
TreeFami | TF316832 |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTSASPEDQN APVGCPKGAR RRRPISVIGG VSLYGTNQTE ELDNLLTQPA
60 70 80 90 100
SRPPMPAHQV PPYKAVSARF RPFTFSQSTP IGLDRVGRRR QMRASNVSSD
110 120 130 140 150
GGTEPSALVD DNGSEEDFSY EDLCQASPRY LQPGGEQLAI NELISDGNVV
160 170 180 190 200
CAEALWDHVT MDDQELGFKA GDVIQVLEAS NKDWWWGRSE DKEAWFPASF
210 220 230 240 250
VRLRVNQEEL SENSSSTPSE EQDEEASQSR HRHCENKQQM RTNVIREIMD
260 270 280 290 300
TERVYIKHLR DICEGYIRQC RKHTGMFTVA QLATIFGNIE DIYKFQRKFL
310 320 330 340 350
KDLEKQYNKE EPHLSEIGSC FLQNQEGFAI YSEYCNNHPG ACLELANLMK
360 370 380 390 400
QGKYRHFFEA CRLLQQMIDI AIDGFLLTPV QKICKYPLQL AELLKYTTQE
410 420 430 440 450
HGDYSNIKAA YEAMKNVACL INERKRKLES IDKIARWQVS IVGWEGLDIL
460 470 480 490 500
DRSSELIHSG ELTKITKQGK SQQRTFFLFD HQLVSCKKDL LRRDMLYYKG
510 520 530 540 550
RLDMDEMELV DLGDGRDKDC NLSVKNAFKL VSRTTDEVYL FCAKKQEDKA
560 570 580 590 600
RWLQACADER RRVQEDKEMG MEISENQKKL AMLNAQKAGH GKSKGYNRCP
610 620 630 640 650
VAPPHQGLHP IHQRHITMPT SVPQQQVFGL AEPKRKSSLF WHTFNRLTPF
RK
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3KQJ8 | J3KQJ8_HUMAN | Spermatogenesis-associated protein ... | SPATA13 | 550 | Annotation score: | ||
E9PFR9 | E9PFR9_HUMAN | Spermatogenesis-associated protein ... | SPATA13 | 512 | Annotation score: | ||
Q5VX67 | Q5VX67_HUMAN | Spermatogenesis-associated protein ... | SPATA13 | 146 | Annotation score: | ||
V9GZ16 | V9GZ16_HUMAN | Spermatogenesis-associated protein ... | SPATA13 | 23 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 304 | E → G in BAG61802 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 520 | C → R in BAG62740 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 537 | E → G in BAG62740 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_030776 | 20 | R → W. Corresponds to variant dbSNP:rs7330736Ensembl. | 1 | |
Natural variantiVAR_080767 | 89 | R → W Found in two consanguineous families with intellectual disability; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs201402934Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041030 | 1 – 96 | MTSAS…MRASN → MVARGEIARFWSLESLHL in isoform 2. 1 PublicationAdd BLAST | 96 | |
Alternative sequenceiVSP_041031 | 1 – 96 | MTSAS…MRASN → MGFIIHIQQVKKQRKKLDQG LILKKEKYRKEKKCASLSFE in isoform 3. 1 PublicationAdd BLAST | 96 | |
Alternative sequenceiVSP_041032 | 1 – 54 | Missing in isoform 4 and isoform 5. 1 PublicationAdd BLAST | 54 | |
Alternative sequenceiVSP_054112 | 1 | M → MTQAAVRPWAPCLENMTTAP NGLGPGPAAPCAGSDLKDAK MVTSLACGNGVCGCSPGGDT DTQEAKLSPAKLVRLFSTSR KRTGAHPERPHSMVLVGNSS TWNTLASFRKMGSFKKLKSS VLKGIQSREGSNACSKGEAS EHGLGKSIPNGAVPGAQASR GSPLAPGPACGALRPAEWGT LDGSDLEDTDDAFQRSTHRS RSLRRAYGLGRICLLDAPQN HATPTIATGQVPAVCEILVR DPENNSMGYRRSKSTDNLAF LKKSSFKRKSTSNLADLRTA HDARVPQRTLSSSSTDSQKL GSGRTKRWRSPIRAKDFDRV FKLVSNVTEAAWRRESPRSG APSPGEASLRLQAHSRLHDD YSRRVSRSTEQDSRRGGAVM HGTTATCTVAPGFGSATSKG PHLDADTAVFPLETKSSWAV ESDSSCTCSSLPSPIVQDVL SKDSCDPNAGSQLTFDPEQP PTPLRPTTPKPQSPQSPQSP GAGSASCHSNHSALSANSEE SEGRAEEPAQREPGPVSLQD PLEATHGDEGSKDLLVNIGV AAGPEEKEKEEVVPDGPWRR SSSQDEERTEAQRTPKRRWG SGRRPRPRPFSDYGQLASRS LSIPEDSVAADPQKEDRVDE DPQASM in isoform 6. Curated | 1 | |
Alternative sequenceiVSP_041033 | 203 – 264 | Missing in isoform 5. 1 PublicationAdd BLAST | 62 | |
Alternative sequenceiVSP_041034 | 595 – 652 | GYNRC…TPFRK → VRLQQVPRGPTAPGPAPHPP APHHYAHKRPPAAGLWPGGT QEEVLALLAHLQQAHPLPEM KTGGCASMELGVKRRN in isoform 4. 1 PublicationAdd BLAST | 58 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BK006072 mRNA Translation: DAA05848.1 AK055770 mRNA Translation: BAB71009.1 AK097217 mRNA Translation: BAC04977.1 Different initiation. AK123031 mRNA Translation: BAG53856.1 AK298717 mRNA Translation: BAG60873.1 AK299981 mRNA Translation: BAG61802.1 AK301149 mRNA Translation: BAG62740.1 AL136963 Genomic DNA No translation available. AL139324 Genomic DNA No translation available. AL359736 Genomic DNA No translation available. AL445985 Genomic DNA No translation available. CH471075 Genomic DNA Translation: EAX08331.1 BC109290 mRNA Translation: AAI09291.1 BC109291 mRNA Translation: AAI09292.1 AK074117 mRNA Translation: BAB84943.1 AK160371 mRNA Translation: BAD18714.1 |
CCDSi | CCDS53857.1 [Q96N96-6] CCDS66517.1 [Q96N96-2] CCDS66518.1 [Q96N96-3] CCDS9305.1 [Q96N96-1] |
RefSeqi | NP_001159743.1, NM_001166271.2 [Q96N96-6] NP_001273721.1, NM_001286792.1 NP_001273722.1, NM_001286793.1 NP_001273723.1, NM_001286794.1 [Q96N96-3] NP_001273724.1, NM_001286795.1 [Q96N96-2] NP_694568.1, NM_153023.3 [Q96N96-1] |
Genome annotation databases
Ensembli | ENST00000343003; ENSP00000343631; ENSG00000182957 [Q96N96-3] ENST00000382095; ENSP00000371527; ENSG00000182957 [Q96N96-1] ENST00000382108; ENSP00000371542; ENSG00000182957 [Q96N96-6] ENST00000399949; ENSP00000382830; ENSG00000182957 [Q96N96-2] ENST00000424834; ENSP00000398560; ENSG00000182957 [Q96N96-6] |
GeneIDi | 221178 |
KEGGi | hsa:221178 |
UCSCi | uc001upg.4, human [Q96N96-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BK006072 mRNA Translation: DAA05848.1 AK055770 mRNA Translation: BAB71009.1 AK097217 mRNA Translation: BAC04977.1 Different initiation. AK123031 mRNA Translation: BAG53856.1 AK298717 mRNA Translation: BAG60873.1 AK299981 mRNA Translation: BAG61802.1 AK301149 mRNA Translation: BAG62740.1 AL136963 Genomic DNA No translation available. AL139324 Genomic DNA No translation available. AL359736 Genomic DNA No translation available. AL445985 Genomic DNA No translation available. CH471075 Genomic DNA Translation: EAX08331.1 BC109290 mRNA Translation: AAI09291.1 BC109291 mRNA Translation: AAI09292.1 AK074117 mRNA Translation: BAB84943.1 AK160371 mRNA Translation: BAD18714.1 |
CCDSi | CCDS53857.1 [Q96N96-6] CCDS66517.1 [Q96N96-2] CCDS66518.1 [Q96N96-3] CCDS9305.1 [Q96N96-1] |
RefSeqi | NP_001159743.1, NM_001166271.2 [Q96N96-6] NP_001273721.1, NM_001286792.1 NP_001273722.1, NM_001286793.1 NP_001273723.1, NM_001286794.1 [Q96N96-3] NP_001273724.1, NM_001286795.1 [Q96N96-2] NP_694568.1, NM_153023.3 [Q96N96-1] |
3D structure databases
SMRi | Q96N96 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 128693, 12 interactors |
IntActi | Q96N96, 12 interactors |
STRINGi | 9606.ENSP00000398560 |
PTM databases
iPTMneti | Q96N96 |
PhosphoSitePlusi | Q96N96 |
Polymorphism and mutation databases
BioMutai | SPATA13 |
DMDMi | 74752049 |
Proteomic databases
jPOSTi | Q96N96 |
MassIVEi | Q96N96 |
MaxQBi | Q96N96 |
PaxDbi | Q96N96 |
PeptideAtlasi | Q96N96 |
PRIDEi | Q96N96 |
ProteomicsDBi | 77489 [Q96N96-1] 77490 [Q96N96-2] 77491 [Q96N96-3] 77492 [Q96N96-4] 77493 [Q96N96-5] |
Protocols and materials databases
Antibodypediai | 49852, 89 antibodies |
Genome annotation databases
Ensembli | ENST00000343003; ENSP00000343631; ENSG00000182957 [Q96N96-3] ENST00000382095; ENSP00000371527; ENSG00000182957 [Q96N96-1] ENST00000382108; ENSP00000371542; ENSG00000182957 [Q96N96-6] ENST00000399949; ENSP00000382830; ENSG00000182957 [Q96N96-2] ENST00000424834; ENSP00000398560; ENSG00000182957 [Q96N96-6] |
GeneIDi | 221178 |
KEGGi | hsa:221178 |
UCSCi | uc001upg.4, human [Q96N96-1] |
Organism-specific databases
CTDi | 221178 |
DisGeNETi | 221178 |
EuPathDBi | HostDB:ENSG00000182957.15 |
GeneCardsi | SPATA13 |
HGNCi | HGNC:23222, SPATA13 |
HPAi | ENSG00000182957, Low tissue specificity |
MIMi | 613324, gene |
neXtProti | NX_Q96N96 |
OpenTargetsi | ENSG00000182957 |
PharmGKBi | PA134912609 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3519, Eukaryota |
GeneTreei | ENSGT00940000154103 |
HOGENOMi | CLU_008436_0_0_1 |
InParanoidi | Q96N96 |
OMAi | KAQSHHN |
OrthoDBi | 65769at2759 |
PhylomeDBi | Q96N96 |
TreeFami | TF316832 |
Enzyme and pathway databases
PathwayCommonsi | Q96N96 |
SIGNORi | Q96N96 |
Miscellaneous databases
BioGRID-ORCSi | 221178, 10 hits in 846 CRISPR screens |
ChiTaRSi | SPATA13, human |
GenomeRNAii | 221178 |
Pharosi | Q96N96, Tbio |
PROi | PR:Q96N96 |
RNActi | Q96N96, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000182957, Expressed in metanephros and 214 other tissues |
ExpressionAtlasi | Q96N96, baseline and differential |
Genevisiblei | Q96N96, HS |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPT13_HUMAN | |
Accessioni | Q96N96Primary (citable) accession number: Q96N96 Secondary accession number(s): A2VEA9 Q8TEK6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 13
Human chromosome 13: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations