UniProtKB - Q96MT8 (CEP63_HUMAN)
Centrosomal protein of 63 kDa
CEP63
Functioni
Required for normal spindle assembly. Plays a key role in mother-centriole-dependent centriole duplication; the function seems also to involve CEP152, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication. Reported to be required for centrosomal recruitment of CEP152; however, this function has been questioned (PubMed:21983783, PubMed:26297806).
Also recruits CDK1 to centrosomes (PubMed:21406398).
Plays a role in DNA damage response. Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes; this leads to the inactivation of spindle assembly and delay in mitotic progression (PubMed:21406398).
3 PublicationsMiscellaneous
GO - Biological processi
- cell division Source: UniProtKB-KW
- centriole replication Source: UniProtKB
- de novo centriole assembly involved in multi-ciliated epithelial cell differentiation Source: GO_Central
- DNA damage checkpoint signaling Source: UniProtKB
- signal transduction in response to DNA damage Source: UniProtKB
- spindle assembly Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division, DNA damage, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q96MT8 |
Reactomei | R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-380259, Loss of Nlp from mitotic centrosomes R-HSA-380270, Recruitment of mitotic centrosome proteins and complexes R-HSA-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-380320, Recruitment of NuMA to mitotic centrosomes R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-8854518, AURKA Activation by TPX2 |
SignaLinki | Q96MT8 |
Names & Taxonomyi
Protein namesi | Recommended name: Centrosomal protein of 63 kDaCuratedShort name: Cep63 |
Gene namesi | Name:CEP63Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25815, CEP63 |
MIMi | 614724, gene |
neXtProti | NX_Q96MT8 |
VEuPathDBi | HostDB:ENSG00000182923 |
Subcellular locationi
Cytoskeleton
- centrosome 3 Publications
- centriole 3 Publications
- centriolar satellite 2 Publications
Note: Colocalizes with CDK5RAP2, CEP152 and WDR62 in a discrete ring around the proximal end of the parental centriole. At this site, a cohesive structure is predicted to engage parental centrioles and procentrioles.3 Publications
Cytoskeleton
- centriole Source: UniProtKB
- centrosome Source: UniProtKB
- spindle pole Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Involvement in diseasei
Seckel syndrome 6 (SCKL6)1 Publication
Keywords - Diseasei
Dwarfism, Mental retardationOrganism-specific databases
DisGeNETi | 80254 |
MalaCardsi | CEP63 |
MIMi | 614728, phenotype |
OpenTargetsi | ENSG00000182923 |
Orphaneti | 2512, Autosomal recessive primary microcephaly |
PharmGKBi | PA142672124 |
Miscellaneous databases
Pharosi | Q96MT8, Tbio |
Genetic variation databases
BioMutai | CEP63 |
DMDMi | 56748851 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089493 | 1 – 703 | Centrosomal protein of 63 kDaAdd BLAST | 703 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 278 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q96MT8 |
jPOSTi | Q96MT8 |
MassIVEi | Q96MT8 |
MaxQBi | Q96MT8 |
PaxDbi | Q96MT8 |
PeptideAtlasi | Q96MT8 |
PRIDEi | Q96MT8 |
ProteomicsDBi | 77406 [Q96MT8-1] 77407 [Q96MT8-2] 77408 [Q96MT8-3] 77409 [Q96MT8-4] |
PTM databases
iPTMneti | Q96MT8 |
PhosphoSitePlusi | Q96MT8 |
Expressioni
Gene expression databases
Bgeei | ENSG00000182923, Expressed in testis and 236 other tissues |
ExpressionAtlasi | Q96MT8, baseline and differential |
Genevisiblei | Q96MT8, HS |
Organism-specific databases
HPAi | ENSG00000182923, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with CEP152 and CDK1; these interactions recruit both ligands to centrosomes (PubMed:21406398).
Interacts with CDK2, CDK5RAP2, WDR62, CEP90, KIAA0753/moonraker and CCDC14. CEP63, CDK5RAP2, CEP152, WDR62 are proposed to form a stepwise assembled complex at the centrosome forming a ring near parental centrioles (PubMed:21406398, PubMed:26297806).
Interacts with CCDC57; the interaction is required for their location to proximal end of centrioles (PubMed:32402286).
4 PublicationsBinary interactionsi
Q96MT8
Isoform 3 [Q96MT8-3]
Protein-protein interaction databases
BioGRIDi | 123200, 179 interactors |
DIPi | DIP-36374N |
IntActi | Q96MT8, 167 interactors |
MINTi | Q96MT8 |
STRINGi | 9606.ENSP00000336524 |
Miscellaneous databases
RNActi | Q96MT8, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q96MT8 |
SMRi | Q96MT8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 22 – 199 | Sequence analysisAdd BLAST | 178 | |
Coiled coili | 242 – 306 | Sequence analysisAdd BLAST | 65 | |
Coiled coili | 353 – 533 | Sequence analysisAdd BLAST | 181 | |
Coiled coili | 676 – 703 | Sequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QRYU, Eukaryota |
GeneTreei | ENSGT00940000153190 |
HOGENOMi | CLU_027471_1_0_1 |
InParanoidi | Q96MT8 |
OMAi | CLDIREQ |
OrthoDBi | 943593at2759 |
PhylomeDBi | Q96MT8 |
TreeFami | TF330595 |
Family and domain databases
InterProi | View protein in InterPro IPR029608, Cep63 IPR031470, Cep63/Deup1_N |
PANTHERi | PTHR18875:SF7, PTHR18875:SF7, 1 hit |
Pfami | View protein in Pfam PF17045, CEP63, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 25 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEALLEGIQN RGHGGGFLTS CEAELQELMK QIDIMVAHKK SEWEGRTHAL
60 70 80 90 100
ETCLKIREQE LKSLRSQLDV THKEVGMLHQ QVEEHEKIKQ EMTMEYKQEL
110 120 130 140 150
KKLHEELCIL KRSYEKLQKK QMREFRGNTK NHREDRSEIE RLTAKIEEFR
160 170 180 190 200
QKSLDWEKQR LIYQQQVSSL EAQRKALAEQ SEIIQAQLVN RKQKLESVEL
210 220 230 240 250
SSQSEIQHLS SKLERANDTI CANELEIERL TMRVNDLVGT SMTVLQEQQQ
260 270 280 290 300
KEEKLRESEK LLEALQEEKR ELKAALQSQE NLIHEARIQK EKLQEKVKAT
310 320 330 340 350
NTQHAVEAIR PREESLAEKK YTSQGQGDLD SVLSQLNFTH TSEDLLQAEV
360 370 380 390 400
TCLEGSLESV SATCKQLSQE LMEKYEELKR MEAHNNEYKA EIKKLKEQIL
410 420 430 440 450
QGEQSYSSAL EGMKMEISHL TQELHQRDIT IASTKGSSSD MEKRLRAEMQ
460 470 480 490 500
KAEDKAVEHK EILDQLESLK LENRHLSEMV MKLELGLHEA KEISLADLQE
510 520 530 540 550
NYIEALNKLV SENQQLQKDL MNTKSQLEIS TQMCKKQNDR IFKPTHSRTT
560 570 580 590 600
EFKNTEFKPT HGQHRHDGIK TEHYKTDLHS PRGQASDSIN PMSRVLSPLS
610 620 630 640 650
PQISPCSSTR SLTSYSLCKT HSLPSALDTN EANFSDTMSE SMNDQEEFIS
660 670 680 690 700
SCSLPVSPLG SIATRFLEEE ELRSHHILER LDAHIEELKR ESEKTVRQFT
ALK
Computationally mapped potential isoform sequencesi
There are 25 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RAY6 | D6RAY6_HUMAN | Centrosomal protein of 63 kDa | CEP63 | 230 | Annotation score: | ||
H0Y900 | H0Y900_HUMAN | Centrosomal protein of 63 kDa | CEP63 | 447 | Annotation score: | ||
A0A3B3IT05 | A0A3B3IT05_HUMAN | Centrosomal protein of 63 kDa | CEP63 | 150 | Annotation score: | ||
D6R9Q4 | D6R9Q4_HUMAN | Centrosomal protein of 63 kDa | CEP63 | 63 | Annotation score: | ||
H0YAE6 | H0YAE6_HUMAN | Centrosomal protein of 63 kDa | CEP63 | 172 | Annotation score: | ||
H0YAL1 | H0YAL1_HUMAN | Centrosomal protein of 63 kDa | CEP63 | 521 | Annotation score: | ||
A0A804HHU4 | A0A804HHU4_HUMAN | Centrosomal protein of 63 kDa | CEP63 | 378 | Annotation score: | ||
A0A804HI22 | A0A804HI22_HUMAN | Centrosomal protein of 63 kDa | CEP63 | 462 | Annotation score: | ||
A0A804HI78 | A0A804HI78_HUMAN | Centrosomal protein of 63 kDa | CEP63 | 205 | Annotation score: | ||
A0A804HIX3 | A0A804HIX3_HUMAN | Centrosomal protein of 63 kDa | CEP63 | 657 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 365 | K → R in BAB14662 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_020604 | 651 | S → L. Corresponds to variant dbSNP:rs1127826EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012251 | 310 – 355 | Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 46 | |
Alternative sequenceiVSP_012252 | 490 – 651 | Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 162 | |
Alternative sequenceiVSP_012253 | 490 – 521 | AKEIS…QKDLM → RWGFTMLSSLVLNFGIQAIR QPQRPKVLELQV in isoform 4. 1 PublicationAdd BLAST | 32 | |
Alternative sequenceiVSP_012254 | 522 – 703 | Missing in isoform 4. 1 PublicationAdd BLAST | 182 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6CSU | X-ray | 2.50 | A/C | 664-703 | [»] | |
6CSV | X-ray | 2.50 | A/B/C/D | 664-703 | [»] | |
AlphaFoldDBi | Q96MT8 | |||||
SMRi | Q96MT8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 123200, 179 interactors |
DIPi | DIP-36374N |
IntActi | Q96MT8, 167 interactors |
MINTi | Q96MT8 |
STRINGi | 9606.ENSP00000336524 |
PTM databases
iPTMneti | Q96MT8 |
PhosphoSitePlusi | Q96MT8 |
Genetic variation databases
BioMutai | CEP63 |
DMDMi | 56748851 |
Proteomic databases
EPDi | Q96MT8 |
jPOSTi | Q96MT8 |
MassIVEi | Q96MT8 |
MaxQBi | Q96MT8 |
PaxDbi | Q96MT8 |
PeptideAtlasi | Q96MT8 |
PRIDEi | Q96MT8 |
ProteomicsDBi | 77406 [Q96MT8-1] 77407 [Q96MT8-2] 77408 [Q96MT8-3] 77409 [Q96MT8-4] |
Protocols and materials databases
Antibodypediai | 46695, 204 antibodies from 25 providers |
DNASUi | 80254 |
Genome annotation databases
Organism-specific databases
CTDi | 80254 |
DisGeNETi | 80254 |
GeneCardsi | CEP63 |
HGNCi | HGNC:25815, CEP63 |
HPAi | ENSG00000182923, Low tissue specificity |
MalaCardsi | CEP63 |
MIMi | 614724, gene 614728, phenotype |
neXtProti | NX_Q96MT8 |
OpenTargetsi | ENSG00000182923 |
Orphaneti | 2512, Autosomal recessive primary microcephaly |
PharmGKBi | PA142672124 |
VEuPathDBi | HostDB:ENSG00000182923 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QRYU, Eukaryota |
GeneTreei | ENSGT00940000153190 |
HOGENOMi | CLU_027471_1_0_1 |
InParanoidi | Q96MT8 |
OMAi | CLDIREQ |
OrthoDBi | 943593at2759 |
PhylomeDBi | Q96MT8 |
TreeFami | TF330595 |
Enzyme and pathway databases
PathwayCommonsi | Q96MT8 |
Reactomei | R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-380259, Loss of Nlp from mitotic centrosomes R-HSA-380270, Recruitment of mitotic centrosome proteins and complexes R-HSA-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-380320, Recruitment of NuMA to mitotic centrosomes R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-8854518, AURKA Activation by TPX2 |
SignaLinki | Q96MT8 |
Miscellaneous databases
BioGRID-ORCSi | 80254, 18 hits in 1084 CRISPR screens |
ChiTaRSi | CEP63, human |
GeneWikii | CEP63 |
GenomeRNAii | 80254 |
Pharosi | Q96MT8, Tbio |
PROi | PR:Q96MT8 |
RNActi | Q96MT8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000182923, Expressed in testis and 236 other tissues |
ExpressionAtlasi | Q96MT8, baseline and differential |
Genevisiblei | Q96MT8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR029608, Cep63 IPR031470, Cep63/Deup1_N |
PANTHERi | PTHR18875:SF7, PTHR18875:SF7, 1 hit |
Pfami | View protein in Pfam PF17045, CEP63, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CEP63_HUMAN | |
Accessioni | Q96MT8Primary (citable) accession number: Q96MT8 Secondary accession number(s): D3DND8 Q9H8N0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 21, 2004 |
Last sequence update: | December 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families