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UniProtKB - Q96MS0 (ROBO3_HUMAN)

Entry version 169 (25 May 2022)
Sequence version 2 (21 Jun 2004)
Previous versions | rss
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Protein

Roundabout homolog 3

Gene

ROBO3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube (By similarity).

In spinal cord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity).

Required for hindbrain axon midline crossing (PubMed:15105459).

By similarity1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDevelopmental protein, Receptor
Biological processChemotaxis, Differentiation, Neurogenesis

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q96MS0

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-428542, Regulation of commissural axon pathfinding by SLIT and ROBO [Q96MS0-1]
R-HSA-9010553, Regulation of expression of SLITs and ROBOs [Q96MS0-1]
R-HSA-9010642, ROBO receptors bind AKAP5 [Q96MS0-1]

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		Q96MS0

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Roundabout homolog 3
Alternative name(s):
Roundabout-like protein 3
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ROBO3
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 11

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:13433, ROBO3

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		608630, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q96MS0

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000154134

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini21 – 891ExtracellularSequence analysisAdd BLAST871
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei892 – 912HelicalSequence analysisAdd BLAST21
Topological domaini913 – 1386CytoplasmicSequence analysisAdd BLAST474

Keywords - Cellular componenti

Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Gaze palsy, familial horizontal, with progressive scoliosis, 1 (HGPPS1)1 Publication
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive neurologic disorder characterized by eye movement abnormalities apparent from birth, childhood-onset progressive scoliosis, distinctive brainstem malformation and defective crossing of some brainstem neuronal pathways.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_0191195L → P in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918275EnsemblClinVar.1
Natural variantiVAR_01912066I → L in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918276EnsemblClinVar.1
Natural variantiVAR_019073319E → K in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918274EnsemblClinVar.1
Natural variantiVAR_019121361G → E in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918270EnsemblClinVar.1
Natural variantiVAR_019074703R → P in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918271EnsemblClinVar.1
Natural variantiVAR_019075705S → P in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918272EnsemblClinVar.1

Keywords - Diseasei

Disease variant

Organism-specific databases

DisGeNET

More...DisGeNETi		64221

MalaCards human disease database

More...MalaCardsi		ROBO3
MIMi607313, phenotype

Open Targets

More...OpenTargetsi		ENSG00000154134

Orphanet; a database dedicated to information on rare diseases and orphan drugs

More...Orphaneti		2744, Horizontal gaze palsy with progressive scoliosis

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA134896648

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q96MS0, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		ROBO3

Domain mapping of disease mutations (DMDM)

More...DMDMi		49036492

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 20Sequence analysisAdd BLAST20
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000003103821 – 1386Roundabout homolog 3Add BLAST1366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section specifies the position and type of each covalently attached glycan group (mono-, di-, or polysaccharide).<p><a href='/help/carbohyd' target='_top'>More...</a></p>Glycosylationi25N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi34N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi41N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi53N-linked (GlcNAc...) asparagineSequence analysis1
<p>This subsection of the PTM / Processing":/help/ptm_processing_section section describes the positions of cysteine residues participating in disulfide bonds.<p><a href='/help/disulfid' target='_top'>More...</a></p>Disulfide bondi85 ↔ 143PROSITE-ProRule annotation
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi187 ↔ 236PROSITE-ProRule annotation
Disulfide bondi279 ↔ 326PROSITE-ProRule annotation
Disulfide bondi368 ↔ 424PROSITE-ProRule annotation
Glycosylationi410N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi459N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi472 ↔ 521PROSITE-ProRule annotation
Glycosylationi503N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi784N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi813N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi820N-linked (GlcNAc...) asparagineSequence analysis1
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei1263PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q96MS0

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q96MS0

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q96MS0

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q96MS0

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q96MS0

PeptideAtlas

More...PeptideAtlasi		Q96MS0

PRoteomics IDEntifications database

More...PRIDEi		Q96MS0

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		77392 [Q96MS0-1]
77393 [Q96MS0-2]

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		Q96MS0, 12 sites, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q96MS0

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q96MS0

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000154134, Expressed in left ovary and 212 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q96MS0, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q96MS0, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000154134, Tissue enhanced (ovary)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Probably interacts with SLIT2.

By similarity

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		122111, 15 interactors

Database of interacting proteins

More...DIPi		DIP-38410N

Protein interaction database and analysis system

More...IntActi		Q96MS0, 3 interactors

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000380903

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q96MS0, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11386
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		Q96MS0

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q96MS0

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini64 – 160Ig-like C2-type 1Add BLAST97
Domaini166 – 253Ig-like C2-type 2Add BLAST88
Domaini258 – 342Ig-like C2-type 3Add BLAST85
Domaini347 – 440Ig-like C2-type 4Add BLAST94
Domaini450 – 531Ig-like C2-type 5Add BLAST82
Domaini558 – 652Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST95
Domaini671 – 766Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96
Domaini771 – 869Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni541 – 563DisorderedSequence analysisAdd BLAST23
Regioni639 – 662DisorderedSequence analysisAdd BLAST24
Regioni965 – 989DisorderedSequence analysisAdd BLAST25
Regioni1028 – 1310DisorderedSequence analysisAdd BLAST283
Regioni1327 – 1386DisorderedSequence analysisAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi967 – 981Polar residuesSequence analysisAdd BLAST15
Compositional biasi1080 – 1094Pro residuesSequence analysisAdd BLAST15
Compositional biasi1122 – 1156Polar residuesSequence analysisAdd BLAST35
Compositional biasi1157 – 1171Pro residuesSequence analysisAdd BLAST15
Compositional biasi1217 – 1238Polar residuesSequence analysisAdd BLAST22
Compositional biasi1263 – 1277Pro residuesSequence analysisAdd BLAST15
Compositional biasi1331 – 1375Polar residuesSequence analysisAdd BLAST45

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the immunoglobulin superfamily. ROBO family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG4222, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000155457

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_003227_5_1_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q96MS0

Identification of Orthologs from Complete Genome Data

More...OMAi		GKAVQMP

Database of Orthologous Groups

More...OrthoDBi		173520at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q96MS0

TreeFam database of animal gene trees

More...TreeFami		TF351053

Family and domain databases

Conserved Domains Database

More...CDDi		cd00063, FN3, 3 hits

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.60.40.10, 8 hits

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR003961, FN3_dom
IPR036116, FN3_sf
IPR007110, Ig-like_dom
IPR036179, Ig-like_dom_sf
IPR013783, Ig-like_fold
IPR013098, Ig_I-set
IPR003599, Ig_sub
IPR003598, Ig_sub2
IPR032987, Robo3

The PANTHER Classification System

More...PANTHERi		PTHR12231:SF236, PTHR12231:SF236, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00041, fn3, 3 hits
PF07679, I-set, 3 hits

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00060, FN3, 3 hits
SM00409, IG, 5 hits
SM00408, IGc2, 5 hits

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF48726, SSF48726, 5 hits
SSF49265, SSF49265, 2 hits

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50853, FN3, 3 hits
PS50835, IG_LIKE, 5 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96MS0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLRYLLKTLL QMNLFADSLA GDISNSSELL LGFNSSLAAL NHTLLPPGDP 
        60         70         80         90        100
SLNGSRVGPE DAMPRIVEQP PDLLVSRGEP ATLPCRAEGR PRPNIEWYKN 
       110        120        130        140        150
GARVATVRED PRAHRLLLPS GALFFPRIVH GRRARPDEGV YTCVARNYLG 
       160        170        180        190        200
AAASRNASLE VAVLRDDFRQ SPGNVVVAVG EPAVLECVPP RGHPEPSVSW 
       210        220        230        240        250
RKDGARLKEE EGRITIRGGK LMMSHTLKSD AGMYVCVASN MAGERESAAA 
       260        270        280        290        300
EVMVLERPSF LRRPVNQVVL ADAPVTFLCE VKGDPPPRLR WRKEDGELPT 
       310        320        330        340        350
GRYEIRSDHS LWIGHVSAED EGTYTCVAEN SVGRAEASGS LSVHVPPQLV 
       360        370        380        390        400
TQPQDQMAAP GESVAFQCET KGNPPPAIFW QKEGSQVLLF PSQSLQPTGR 
       410        420        430        440        450
FSVSPRGQLN ITAVQRGDAG YYVCQAVSVA GSILAKALLE IKGASLDGLP 
       460        470        480        490        500
PVILQGPANQ TLVLGSSVWL PCRVTGNPQP SVRWKKDGQW LQGDDLQFKT 
       510        520        530        540        550
MANGTLYIAN VQEMDMGFYS CVAKSSTGEA TWSGWLKMRE DWGVSPDPPT 
       560        570        580        590        600
EPSSPPGAPS QPVVTEITKN SITLTWKPNP QTGAAVTSYV IEAFSPAAGN 
       610        620        630        640        650
TWRTVADGVQ LETHTVSGLQ PNTIYLFLVR AVGAWGLSEP SPVSEPVRTQ 
       660        670        680        690        700
DSSPSRPVED PWRGQQGLAE VAVRLQEPIV LGPRTLQVSW TVDGPVQLVQ 
       710        720        730        740        750
GFRVSWRVAG PEGGSWTMLD LQSPSQQSTV LRGLPPGTQI QIKVQAQGQE 
       760        770        780        790        800
GLGAESLSVT RSIPEEAPSG PPQGVAVALG GDGNSSITVS WEPPLPSQQN 
       810        820        830        840        850
GVITEYQIWC LGNESRFHLN RSAAGWARSA MLRGLVPGLL YRTLVAAATS 
       860        870        880        890        900
AGVGVPSAPV LVQLPSPPDL EPGLEVGAGL AVRLARVLRE PAFLAGSGAA 
       910        920        930        940        950
CGALLLGLCA ALYWRRKQRK ELSHYTASFA YTPAVSFPHS EGLSGASSRP 
       960        970        980        990       1000
PMGLGPAPYS WLADSWPHPS RSPSAQEPRG SCCPSNPDPD DRYYNEAGIS 
      1010       1020       1030       1040       1050
LYLAQTARGT AAPGEGPVYS TIDPAGEELQ TFHGGFPQHP SGDLGPWSQY 
      1060       1070       1080       1090       1100
APPEWSQGDS GAKGGKVKLL GKPVQMPSLN WPEALPPPPP SCELSCLEGP 
      1110       1120       1130       1140       1150
EEELEGSSEP EEWCPPMPER SHLTEPSSSG GCLVTPSRRE TPSPTPSYGQ 
      1160       1170       1180       1190       1200
QSTATLTPSP PDPPQPPTDM PHLHQMPRRV PLGPSSPLSV SQPMLGIREA 
      1210       1220       1230       1240       1250
RPAGLGAGPA ASPHLSPSPA PSTASSAPGR TWQGNGEMTP PLQGPRARFR 
      1260       1270       1280       1290       1300
KKPKALPYRR ENSPGDLPPP PLPPPEEEAS WALELRAAGS MSSLERERSG 
      1310       1320       1330       1340       1350
ERKAVQAVPL AAQRVLHPDE EAWLPYSRPS FLSRGQGTST CSTAGSNSSR 
      1360       1370       1380 
GSSSSRGSRG PGRSRSRSQS RSQSQRPGQK RREEPR
Length:1,386
Mass (Da):148,209
Last modified:June 21, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i955A7300A2BDA27F
GO
Isoform 2 (identifier: Q96MS0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1025-1034: AGEELQTFHG → LTTPLLILTT
     1035-1386: Missing.

Show »
Length:1,034
Mass (Da):110,676
Checksum:i95EB31C89F0531E5
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5GWJ5F5GWJ5_HUMAN
Roundabout homolog 3
ROBO3
1,364Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F5H0K7F5H0K7_HUMAN
Roundabout homolog 3
ROBO3
149Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0191195L → P in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918275EnsemblClinVar.1
Natural variantiVAR_01912066I → L in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918276EnsemblClinVar.1
Natural variantiVAR_019073319E → K in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918274EnsemblClinVar.1
Natural variantiVAR_019121361G → E in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918270EnsemblClinVar.1
Natural variantiVAR_053642416R → H. Corresponds to variant dbSNP:rs3862618EnsemblClinVar.1
Natural variantiVAR_034474423V → M1 PublicationCorresponds to variant dbSNP:rs4935898EnsemblClinVar.1
Natural variantiVAR_019074703R → P in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918271EnsemblClinVar.1
Natural variantiVAR_019075705S → P in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918272EnsemblClinVar.1
Natural variantiVAR_062145868P → L1 PublicationCorresponds to variant dbSNP:rs55706177EnsemblClinVar.1
Natural variantiVAR_0344751369Q → R. Corresponds to variant dbSNP:rs35723495Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0106501025 – 1034AGEELQTFHG → LTTPLLILTT in isoform 2. 1 Publication10
Alternative sequenceiVSP_0106511035 – 1386Missing in isoform 2. 1 PublicationAdd BLAST352

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AY509035 mRNA Translation: AAS91662.1
AK056544 mRNA Translation: BAB71212.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS44755.1 [Q96MS0-1]

NCBI Reference Sequences

More...RefSeqi		NP_071765.2, NM_022370.3 [Q96MS0-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000397801.6; ENSP00000380903.1; ENSG00000154134.15

Database of genes from NCBI RefSeq genomes

More...GeneIDi		64221

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:64221

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000397801.6; ENSP00000380903.1; NM_022370.4; NP_071765.2

UCSC genome browser

More...UCSCi		uc001qbc.4, human [Q96MS0-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY509035 mRNA Translation: AAS91662.1
AK056544 mRNA Translation: BAB71212.1
CCDSiCCDS44755.1 [Q96MS0-1]
RefSeqiNP_071765.2, NM_022370.3 [Q96MS0-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6POGX-ray2.75A551-663[»]
6POKX-ray1.80A664-870[»]
6POLX-ray1.80A/C/E549-652[»]
AlphaFoldDBiQ96MS0
SMRiQ96MS0
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi122111, 15 interactors
DIPiDIP-38410N
IntActiQ96MS0, 3 interactors
STRINGi9606.ENSP00000380903

PTM databases

GlyGeniQ96MS0, 12 sites, 1 O-linked glycan (1 site)
iPTMnetiQ96MS0
PhosphoSitePlusiQ96MS0

Genetic variation databases

BioMutaiROBO3
DMDMi49036492

Proteomic databases

EPDiQ96MS0
jPOSTiQ96MS0
MassIVEiQ96MS0
MaxQBiQ96MS0
PaxDbiQ96MS0
PeptideAtlasiQ96MS0
PRIDEiQ96MS0
ProteomicsDBi77392 [Q96MS0-1]
77393 [Q96MS0-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		2550, 201 antibodies from 33 providers

The DNASU plasmid repository

More...DNASUi		64221

Genome annotation databases

EnsembliENST00000397801.6; ENSP00000380903.1; ENSG00000154134.15
GeneIDi64221
KEGGihsa:64221
MANE-SelectiENST00000397801.6; ENSP00000380903.1; NM_022370.4; NP_071765.2
UCSCiuc001qbc.4, human [Q96MS0-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		64221
DisGeNETi64221

GeneCards: human genes, protein and diseases

More...GeneCardsi		ROBO3
HGNCiHGNC:13433, ROBO3
HPAiENSG00000154134, Tissue enhanced (ovary)
MalaCardsiROBO3
MIMi607313, phenotype
608630, gene
neXtProtiNX_Q96MS0
OpenTargetsiENSG00000154134
Orphaneti2744, Horizontal gaze palsy with progressive scoliosis
PharmGKBiPA134896648
VEuPathDBiHostDB:ENSG00000154134

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG4222, Eukaryota
GeneTreeiENSGT00940000155457
HOGENOMiCLU_003227_5_1_1
InParanoidiQ96MS0
OMAiGKAVQMP
OrthoDBi173520at2759
PhylomeDBiQ96MS0
TreeFamiTF351053

Enzyme and pathway databases

PathwayCommonsiQ96MS0
ReactomeiR-HSA-428542, Regulation of commissural axon pathfinding by SLIT and ROBO [Q96MS0-1]
R-HSA-9010553, Regulation of expression of SLITs and ROBOs [Q96MS0-1]
R-HSA-9010642, ROBO receptors bind AKAP5 [Q96MS0-1]
SignaLinkiQ96MS0

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		64221, 5 hits in 1069 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		ROBO3, human

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		ROBO3

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		64221
PharosiQ96MS0, Tbio

Protein Ontology

More...PROi		PR:Q96MS0
RNActiQ96MS0, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000154134, Expressed in left ovary and 212 other tissues
ExpressionAtlasiQ96MS0, baseline and differential
GenevisibleiQ96MS0, HS

Family and domain databases

CDDicd00063, FN3, 3 hits
Gene3Di2.60.40.10, 8 hits
InterProiView protein in InterPro
IPR003961, FN3_dom
IPR036116, FN3_sf
IPR007110, Ig-like_dom
IPR036179, Ig-like_dom_sf
IPR013783, Ig-like_fold
IPR013098, Ig_I-set
IPR003599, Ig_sub
IPR003598, Ig_sub2
IPR032987, Robo3
PANTHERiPTHR12231:SF236, PTHR12231:SF236, 1 hit
PfamiView protein in Pfam
PF00041, fn3, 3 hits
PF07679, I-set, 3 hits
SMARTiView protein in SMART
SM00060, FN3, 3 hits
SM00409, IG, 5 hits
SM00408, IGc2, 5 hits
SUPFAMiSSF48726, SSF48726, 5 hits
SSF49265, SSF49265, 2 hits
PROSITEiView protein in PROSITE
PS50853, FN3, 3 hits
PS50835, IG_LIKE, 5 hits

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiROBO3_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q96MS0
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: May 25, 2022
This is version 169 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families
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