UniProtKB - Q96MS0 (ROBO3_HUMAN)

BLAST

>sp|Q96MS0|ROBO3_HUMAN Roundabout homolog 3 OS=Homo sapiens OX=9606 GN=ROBO3 PE=1 SV=2
MLRYLLKTLLQMNLFADSLAGDISNSSELLLGFNSSLAALNHTLLPPGDPSLNGSRVGPE
DAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATVREDPRAHRLLLPS
GALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVG
EPAVLECVPPRGHPEPSVSWRKDGARLKEEEGRITIRGGKLMMSHTLKSDAGMYVCVASN
MAGERESAAAEVMVLERPSFLRRPVNQVVLADAPVTFLCEVKGDPPPRLRWRKEDGELPT
GRYEIRSDHSLWIGHVSAEDEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAP
GESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPTGRFSVSPRGQLNITAVQRGDAG
YYVCQAVSVAGSILAKALLEIKGASLDGLPPVILQGPANQTLVLGSSVWLPCRVTGNPQP
SVRWKKDGQWLQGDDLQFKTMANGTLYIANVQEMDMGFYSCVAKSSTGEATWSGWLKMRE
DWGVSPDPPTEPSSPPGAPSQPVVTEITKNSITLTWKPNPQTGAAVTSYVIEAFSPAAGN
TWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPVRTQDSSPSRPVED
PWRGQQGLAEVAVRLQEPIVLGPRTLQVSWTVDGPVQLVQGFRVSWRVAGPEGGSWTMLD
LQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGAESLSVTRSIPEEAPSGPPQGVAVALG
GDGNSSITVSWEPPLPSQQNGVITEYQIWCLGNESRFHLNRSAAGWARSAMLRGLVPGLL
YRTLVAAATSAGVGVPSAPVLVQLPSPPDLEPGLEVGAGLAVRLARVLREPAFLAGSGAA
CGALLLGLCAALYWRRKQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYS
WLADSWPHPSRSPSAQEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPGEGPVYS
TIDPAGEELQTFHGGFPQHPSGDLGPWSQYAPPEWSQGDSGAKGGKVKLLGKPVQMPSLN
WPEALPPPPPSCELSCLEGPEEELEGSSEPEEWCPPMPERSHLTEPSSSGGCLVTPSRRE
TPSPTPSYGQQSTATLTPSPPDPPQPPTDMPHLHQMPRRVPLGPSSPLSVSQPMLGIREA
RPAGLGAGPAASPHLSPSPAPSTASSAPGRTWQGNGEMTPPLQGPRARFRKKPKALPYRR
ENSPGDLPPPPLPPPEEEASWALELRAAGSMSSLERERSGERKAVQAVPLAAQRVLHPDE
EAWLPYSRPSFLSRGQGTSTCSTAGSNSSRGSSSSRGSRGPGRSRSRSQSRSQSQRPGQK
RREEPR

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History
Entry version 148 (07 Nov 2018)
Sequence version 2 (21 Jun 2004)
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Protein

Roundabout homolog 3

Gene

ROBO3

Organism

Homo sapiens (Human)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing.By similarity1 Publication

GO - Molecular functionⁱ

cell-cell adhesion mediator activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

axon guidance
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
axon midline choice point recognition Source: UniProtKB
chemoattraction of axon Source: Ensembl
commissural neuron axon guidance Source: Ensembl
dendrite self-avoidance
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
homophilic cell adhesion via plasma membrane adhesion molecules
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
Roundabout signaling pathway Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein, Receptor
Biological process	Chemotaxis, Differentiation, Neurogenesis

Enzyme and pathway databases

Reactomeⁱ	R-HSA-428542 Regulation of commissural axon pathfinding by SLIT and ROBO R-HSA-9010553 Regulation of expression of SLITs and ROBOs R-HSA-9010642 ROBO receptors bind AKAP5

Reactomeⁱ

R-HSA-428542 Regulation of commissural axon pathfinding by SLIT and ROBO
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9010642 ROBO receptors bind AKAP5

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Roundabout homolog 3 Alternative name(s): Roundabout-like protein 3
Gene namesⁱ	Name:ROBO3
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 11

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000154134.14
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:13433 ROBO3
MIMⁱ	608630 gene
neXtProtⁱ	NX_Q96MS0

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	21 – 891	ExtracellularSequence analysisAdd BLAST	871
Transmembraneⁱ	892 – 912	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	913 – 1386	CytoplasmicSequence analysisAdd BLAST	474

Keywords - Cellular componentⁱ

Membrane

Pathology & Biotechⁱ

Involvement in diseaseⁱ

Gaze palsy, familial horizontal, with progressive scoliosis, 1 (HGPPS1)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionAn autosomal recessive neurologic disorder characterized by eye movement abnormalities apparent from birth, childhood-onset progressive scoliosis, distinctive brainstem malformation and defective crossing of some brainstem neuronal pathways.

Keywords - Diseaseⁱ

Disease mutation

Organism-specific databases

DisGeNET More...DisGeNETⁱ	64221
MalaCards human disease database More...MalaCardsⁱ	ROBO3
MIMⁱ	607313 phenotype
Open Targets More...OpenTargetsⁱ	ENSG00000154134
Orphanetⁱ	2744 Horizontal gaze palsy with progressive scoliosis
PharmGKBⁱ	PA134896648

Polymorphism and mutation databases

BioMutaⁱ	ROBO3
DMDMⁱ	49036492

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 20	Sequence analysisAdd BLAST		20
ChainⁱPRO_0000031038	21 – 1386	Roundabout homolog 3Add BLAST		1366

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	25	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	34	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	41	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	53	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	85 ↔ 143	PROSITE-ProRule annotation
Glycosylationⁱ	156	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	187 ↔ 236	PROSITE-ProRule annotation
Disulfide bondⁱ	279 ↔ 326	PROSITE-ProRule annotation
Disulfide bondⁱ	368 ↔ 424	PROSITE-ProRule annotation
Glycosylationⁱ	410	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	459	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	472 ↔ 521	PROSITE-ProRule annotation
Glycosylationⁱ	503	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	784	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	813	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	820	N-linked (GlcNAc...) asparagineSequence analysis	1
Modified residueⁱ	1263	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDⁱ	Q96MS0
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q96MS0
PaxDbⁱ	Q96MS0
PeptideAtlas More...PeptideAtlasⁱ	Q96MS0
PRIDEⁱ	Q96MS0
ProteomicsDBⁱ	77392 77393 [Q96MS0-2]

PTM databases

iPTMnetⁱ	Q96MS0
PhosphoSitePlusⁱ	Q96MS0

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000154134 Expressed in 198 organ(s), highest expression level in left ovary
CleanExⁱ	HS_ROBO3
ExpressionAtlasⁱ	Q96MS0 baseline and differential
Genevisibleⁱ	Q96MS0 HS

Interactionⁱ

Subunit structureⁱ

Probably interacts with SLIT2.By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
NELL2	Q99435-1	3	EBI-1220465,EBI-16185191

With

Entry

#Exp.

IntAct

Notes

NELL2

Q99435-1

EBI-1220465,EBI-16185191

GO - Molecular functionⁱ

cell-cell adhesion mediator activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	122111, 8 interactors
Database of interacting proteins More...DIPⁱ	DIP-38410N
IntActⁱ	Q96MS0, 2 interactors
STRINGⁱ	9606.ENSP00000380903

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q96MS0
SMRⁱ	Q96MS0
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	64 – 160	Ig-like C2-type 1Add BLAST	97
Domainⁱ	166 – 253	Ig-like C2-type 2Add BLAST	88
Domainⁱ	258 – 342	Ig-like C2-type 3Add BLAST	85
Domainⁱ	347 – 440	Ig-like C2-type 4Add BLAST	94
Domainⁱ	450 – 531	Ig-like C2-type 5Add BLAST	82
Domainⁱ	558 – 652	Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST	95
Domainⁱ	671 – 766	Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST	96
Domainⁱ	771 – 869	Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST	99

Sequence similaritiesⁱ

Belongs to the immunoglobulin superfamily. ROBO family.Curated

Keywords - Domainⁱ

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG4222 Eukaryota ENOG410XPQS LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00930000150875
HOGENOMⁱ	HOG000010267
HOVERGENⁱ	HBG073476
InParanoidⁱ	Q96MS0
KEGG Orthology (KO) More...KOⁱ	K06755
OMAⁱ	DPWRGQR
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G00CG
PhylomeDBⁱ	Q96MS0
TreeFamⁱ	TF351053

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00063 FN3, 3 hits
Gene3Dⁱ	2.60.40.10, 8 hits
InterProⁱ	View protein in InterPro IPR003961 FN3_dom IPR036116 FN3_sf IPR007110 Ig-like_dom IPR036179 Ig-like_dom_sf IPR013783 Ig-like_fold IPR013098 Ig_I-set IPR003599 Ig_sub IPR003598 Ig_sub2 IPR032987 Robo3
PANTHERⁱ	PTHR44096:SF3 PTHR44096:SF3, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00041 fn3, 3 hits PF07679 I-set, 3 hits
SMARTⁱ	View protein in SMART SM00060 FN3, 3 hits SM00409 IG, 5 hits SM00408 IGc2, 5 hits
SUPFAMⁱ	SSF48726 SSF48726, 5 hits SSF49265 SSF49265, 2 hits
PROSITEⁱ	View protein in PROSITE PS50853 FN3, 3 hits PS50835 IG_LIKE, 5 hits

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q96MS0-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRYLLKTLL QMNLFADSLA GDISNSSELL LGFNSSLAAL NHTLLPPGDP 
        60         70         80         90        100
SLNGSRVGPE DAMPRIVEQP PDLLVSRGEP ATLPCRAEGR PRPNIEWYKN 
       110        120        130        140        150
GARVATVRED PRAHRLLLPS GALFFPRIVH GRRARPDEGV YTCVARNYLG 
       160        170        180        190        200
AAASRNASLE VAVLRDDFRQ SPGNVVVAVG EPAVLECVPP RGHPEPSVSW 
       210        220        230        240        250
RKDGARLKEE EGRITIRGGK LMMSHTLKSD AGMYVCVASN MAGERESAAA 
       260        270        280        290        300
EVMVLERPSF LRRPVNQVVL ADAPVTFLCE VKGDPPPRLR WRKEDGELPT 
       310        320        330        340        350
GRYEIRSDHS LWIGHVSAED EGTYTCVAEN SVGRAEASGS LSVHVPPQLV 
       360        370        380        390        400
TQPQDQMAAP GESVAFQCET KGNPPPAIFW QKEGSQVLLF PSQSLQPTGR 
       410        420        430        440        450
FSVSPRGQLN ITAVQRGDAG YYVCQAVSVA GSILAKALLE IKGASLDGLP 
       460        470        480        490        500
PVILQGPANQ TLVLGSSVWL PCRVTGNPQP SVRWKKDGQW LQGDDLQFKT 
       510        520        530        540        550
MANGTLYIAN VQEMDMGFYS CVAKSSTGEA TWSGWLKMRE DWGVSPDPPT 
       560        570        580        590        600
EPSSPPGAPS QPVVTEITKN SITLTWKPNP QTGAAVTSYV IEAFSPAAGN 
       610        620        630        640        650
TWRTVADGVQ LETHTVSGLQ PNTIYLFLVR AVGAWGLSEP SPVSEPVRTQ 
       660        670        680        690        700
DSSPSRPVED PWRGQQGLAE VAVRLQEPIV LGPRTLQVSW TVDGPVQLVQ 
       710        720        730        740        750
GFRVSWRVAG PEGGSWTMLD LQSPSQQSTV LRGLPPGTQI QIKVQAQGQE 
       760        770        780        790        800
GLGAESLSVT RSIPEEAPSG PPQGVAVALG GDGNSSITVS WEPPLPSQQN 
       810        820        830        840        850
GVITEYQIWC LGNESRFHLN RSAAGWARSA MLRGLVPGLL YRTLVAAATS 
       860        870        880        890        900
AGVGVPSAPV LVQLPSPPDL EPGLEVGAGL AVRLARVLRE PAFLAGSGAA 
       910        920        930        940        950
CGALLLGLCA ALYWRRKQRK ELSHYTASFA YTPAVSFPHS EGLSGASSRP 
       960        970        980        990       1000
PMGLGPAPYS WLADSWPHPS RSPSAQEPRG SCCPSNPDPD DRYYNEAGIS 
      1010       1020       1030       1040       1050
LYLAQTARGT AAPGEGPVYS TIDPAGEELQ TFHGGFPQHP SGDLGPWSQY 
      1060       1070       1080       1090       1100
APPEWSQGDS GAKGGKVKLL GKPVQMPSLN WPEALPPPPP SCELSCLEGP 
      1110       1120       1130       1140       1150
EEELEGSSEP EEWCPPMPER SHLTEPSSSG GCLVTPSRRE TPSPTPSYGQ 
      1160       1170       1180       1190       1200
QSTATLTPSP PDPPQPPTDM PHLHQMPRRV PLGPSSPLSV SQPMLGIREA 
      1210       1220       1230       1240       1250
RPAGLGAGPA ASPHLSPSPA PSTASSAPGR TWQGNGEMTP PLQGPRARFR 
      1260       1270       1280       1290       1300
KKPKALPYRR ENSPGDLPPP PLPPPEEEAS WALELRAAGS MSSLERERSG 
      1310       1320       1330       1340       1350
ERKAVQAVPL AAQRVLHPDE EAWLPYSRPS FLSRGQGTST CSTAGSNSSR 
      1360       1370       1380 
GSSSSRGSRG PGRSRSRSQS RSQSQRPGQK RREEPR

Length:1,386

Mass (Da):148,209

Last modified:June 21, 2004 - v2

Checksum:ⁱ955A7300A2BDA27F

Isoform 2 (identifier: Q96MS0-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1025-1034: AGEELQTFHG → LTTPLLILTT
1035-1386: Missing.

« Hide

        10         20         30         40         50
MLRYLLKTLL QMNLFADSLA GDISNSSELL LGFNSSLAAL NHTLLPPGDP 
        60         70         80         90        100
SLNGSRVGPE DAMPRIVEQP PDLLVSRGEP ATLPCRAEGR PRPNIEWYKN 
       110        120        130        140        150
GARVATVRED PRAHRLLLPS GALFFPRIVH GRRARPDEGV YTCVARNYLG 
       160        170        180        190        200
AAASRNASLE VAVLRDDFRQ SPGNVVVAVG EPAVLECVPP RGHPEPSVSW 
       210        220        230        240        250
RKDGARLKEE EGRITIRGGK LMMSHTLKSD AGMYVCVASN MAGERESAAA 
       260        270        280        290        300
EVMVLERPSF LRRPVNQVVL ADAPVTFLCE VKGDPPPRLR WRKEDGELPT 
       310        320        330        340        350
GRYEIRSDHS LWIGHVSAED EGTYTCVAEN SVGRAEASGS LSVHVPPQLV 
       360        370        380        390        400
TQPQDQMAAP GESVAFQCET KGNPPPAIFW QKEGSQVLLF PSQSLQPTGR 
       410        420        430        440        450
FSVSPRGQLN ITAVQRGDAG YYVCQAVSVA GSILAKALLE IKGASLDGLP 
       460        470        480        490        500
PVILQGPANQ TLVLGSSVWL PCRVTGNPQP SVRWKKDGQW LQGDDLQFKT 
       510        520        530        540        550
MANGTLYIAN VQEMDMGFYS CVAKSSTGEA TWSGWLKMRE DWGVSPDPPT 
       560        570        580        590        600
EPSSPPGAPS QPVVTEITKN SITLTWKPNP QTGAAVTSYV IEAFSPAAGN 
       610        620        630        640        650
TWRTVADGVQ LETHTVSGLQ PNTIYLFLVR AVGAWGLSEP SPVSEPVRTQ 
       660        670        680        690        700
DSSPSRPVED PWRGQQGLAE VAVRLQEPIV LGPRTLQVSW TVDGPVQLVQ 
       710        720        730        740        750
GFRVSWRVAG PEGGSWTMLD LQSPSQQSTV LRGLPPGTQI QIKVQAQGQE 
       760        770        780        790        800
GLGAESLSVT RSIPEEAPSG PPQGVAVALG GDGNSSITVS WEPPLPSQQN 
       810        820        830        840        850
GVITEYQIWC LGNESRFHLN RSAAGWARSA MLRGLVPGLL YRTLVAAATS 
       860        870        880        890        900
AGVGVPSAPV LVQLPSPPDL EPGLEVGAGL AVRLARVLRE PAFLAGSGAA 
       910        920        930        940        950
CGALLLGLCA ALYWRRKQRK ELSHYTASFA YTPAVSFPHS EGLSGASSRP 
       960        970        980        990       1000
PMGLGPAPYS WLADSWPHPS RSPSAQEPRG SCCPSNPDPD DRYYNEAGIS 
      1010       1020       1030 
LYLAQTARGT AAPGEGPVYS TIDPLTTPLL ILTT

Show »

Length:1,034

Mass (Da):110,676

Checksum:ⁱ95EB31C89F0531E5

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F5GWJ5	F5GWJ5_HUMAN	Roundabout homolog 3 Roundabout homolog 3	ROBO3	1,364	Annotation score:
F5H0K7	F5H0K7_HUMAN	Roundabout homolog 3 Roundabout homolog 3	ROBO3	149	Annotation score:

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_019119	5	L → P in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918275EnsemblClinVar.	1
Natural variantⁱVAR_019120	66	I → L in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918276EnsemblClinVar.	1
Natural variantⁱVAR_019073	319	E → K in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918274EnsemblClinVar.	1
Natural variantⁱVAR_019121	361	G → E in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918270EnsemblClinVar.	1
Natural variantⁱVAR_053642	416	R → H. Corresponds to variant dbSNP:rs3862618EnsemblClinVar.	1
Natural variantⁱVAR_034474	423	V → M1 PublicationCorresponds to variant dbSNP:rs4935898EnsemblClinVar.	1
Natural variantⁱVAR_019074	703	R → P in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918271EnsemblClinVar.	1
Natural variantⁱVAR_019075	705	S → P in HGPPS1. 1 PublicationCorresponds to variant dbSNP:rs121918272EnsemblClinVar.	1
Natural variantⁱVAR_062145	868	P → L1 PublicationCorresponds to variant dbSNP:rs55706177EnsemblClinVar.	1
Natural variantⁱVAR_034475	1369	Q → R. Corresponds to variant dbSNP:rs35723495Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_010650	1025 – 1034	AGEELQTFHG → LTTPLLILTT in isoform 2. 1 Publication		10
Alternative sequenceⁱVSP_010651	1035 – 1386	Missing in isoform 2. 1 PublicationAdd BLAST		352

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY509035 mRNA Translation: AAS91662.1 AK056544 mRNA Translation: BAB71212.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS44755.1 [Q96MS0-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_071765.2, NM_022370.3 [Q96MS0-1]
UniGeneⁱ	Hs.435621

Genome annotation databases

Ensemblⁱ	ENST00000397801; ENSP00000380903; ENSG00000154134 [Q96MS0-1]
GeneIDⁱ	64221
KEGGⁱ	hsa:64221
UCSC genome browser More...UCSCⁱ	uc001qbc.4 human [Q96MS0-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q96MS0	Roundabout, axon guidance receptor, homolog 3 (Drosophila), isoform CRA_c		HUMAN		311	UniRef100_Q96MS0
Roundabout homolog 3		HUMAN		149
Roundabout, axon guidance receptor, homolog 3 (Drosophila), isoform CRA_b		HUMAN		435

Protein	Similar proteins		Species	Score	Length	Source
Q96MS0	Roundabout, axon guidance receptor, homolog 3 (Drosophila), isoform CRA_c		HUMAN		311	UniRef90_Q96MS0
Roundabout guidance receptor 3		PONAB		1493
Uncharacterized protein		PROCO		1385
Uncharacterized protein		COLAP		1384
Uncharacterized protein		RHIRO		1384
+75

Protein	Similar proteins		Species	Score	Length	Source
Q96MS0	Roundabout homolog 3		MOUSE		1366	UniRef50_Q96MS0
Roundabout, axon guidance receptor, homolog 3 (Drosophila), isoform CRA_c		HUMAN		311
Roundabout guidance receptor 3		PONAB		1493
Uncharacterized protein		PROCO		1385
Uncharacterized protein		PAPAN		1384
+197
Q96MS0-2	Uncharacterized protein		POEFO		1042	UniRef50_Q96MS0-2
Uncharacterized protein		ONCMY		956
Chromosome 16 SCAF15002, whole genome shotgun sequence (Fragment)		TETNG		1098
Roundabout		FUNHE		1072
Robo3 (Fragment)		DANRE		956
+5

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY509035 mRNA Translation: AAS91662.1 AK056544 mRNA Translation: BAB71212.1
CCDSⁱ	CCDS44755.1 [Q96MS0-1]
RefSeqⁱ	NP_071765.2, NM_022370.3 [Q96MS0-1]
UniGeneⁱ	Hs.435621

3D structure databases

ProteinModelPortalⁱ	Q96MS0
SMRⁱ	Q96MS0
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	122111, 8 interactors
DIPⁱ	DIP-38410N
IntActⁱ	Q96MS0, 2 interactors
STRINGⁱ	9606.ENSP00000380903

PTM databases

iPTMnetⁱ	Q96MS0
PhosphoSitePlusⁱ	Q96MS0

Polymorphism and mutation databases

BioMutaⁱ	ROBO3
DMDMⁱ	49036492

Proteomic databases

EPDⁱ	Q96MS0
MaxQBⁱ	Q96MS0
PaxDbⁱ	Q96MS0
PeptideAtlasⁱ	Q96MS0
PRIDEⁱ	Q96MS0
ProteomicsDBⁱ	77392 77393 [Q96MS0-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	64221
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000397801; ENSP00000380903; ENSG00000154134 [Q96MS0-1]
GeneIDⁱ	64221
KEGGⁱ	hsa:64221
UCSCⁱ	uc001qbc.4 human [Q96MS0-1]

Organism-specific databases

CTDⁱ	64221
DisGeNETⁱ	64221
EuPathDBⁱ	HostDB:ENSG00000154134.14
GeneCardsⁱ	ROBO3
HGNCⁱ	HGNC:13433 ROBO3
MalaCardsⁱ	ROBO3
MIMⁱ	607313 phenotype 608630 gene
neXtProtⁱ	NX_Q96MS0
OpenTargetsⁱ	ENSG00000154134
Orphanetⁱ	2744 Horizontal gaze palsy with progressive scoliosis
PharmGKBⁱ	PA134896648
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG4222 Eukaryota ENOG410XPQS LUCA
GeneTreeⁱ	ENSGT00930000150875
HOGENOMⁱ	HOG000010267
HOVERGENⁱ	HBG073476
InParanoidⁱ	Q96MS0
KOⁱ	K06755
OMAⁱ	DPWRGQR
OrthoDBⁱ	EOG091G00CG
PhylomeDBⁱ	Q96MS0
TreeFamⁱ	TF351053

Enzyme and pathway databases

Reactomeⁱ	R-HSA-428542 Regulation of commissural axon pathfinding by SLIT and ROBO R-HSA-9010553 Regulation of expression of SLITs and ROBOs R-HSA-9010642 ROBO receptors bind AKAP5

Reactomeⁱ

R-HSA-428542 Regulation of commissural axon pathfinding by SLIT and ROBO
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9010642 ROBO receptors bind AKAP5

Miscellaneous databases

ChiTaRSⁱ	ROBO3 human
GeneWikiⁱ	ROBO3
GenomeRNAiⁱ	64221
Protein Ontology More...PROⁱ	PR:Q96MS0
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000154134 Expressed in 198 organ(s), highest expression level in left ovary
CleanExⁱ	HS_ROBO3
ExpressionAtlasⁱ	Q96MS0 baseline and differential
Genevisibleⁱ	Q96MS0 HS

Family and domain databases

CDDⁱ	cd00063 FN3, 3 hits
Gene3Dⁱ	2.60.40.10, 8 hits
InterProⁱ	View protein in InterPro IPR003961 FN3_dom IPR036116 FN3_sf IPR007110 Ig-like_dom IPR036179 Ig-like_dom_sf IPR013783 Ig-like_fold IPR013098 Ig_I-set IPR003599 Ig_sub IPR003598 Ig_sub2 IPR032987 Robo3
PANTHERⁱ	PTHR44096:SF3 PTHR44096:SF3, 1 hit
Pfamⁱ	View protein in Pfam PF00041 fn3, 3 hits PF07679 I-set, 3 hits
SMARTⁱ	View protein in SMART SM00060 FN3, 3 hits SM00409 IG, 5 hits SM00408 IGc2, 5 hits
SUPFAMⁱ	SSF48726 SSF48726, 5 hits SSF49265 SSF49265, 2 hits
PROSITEⁱ	View protein in PROSITE PS50853 FN3, 3 hits PS50835 IG_LIKE, 5 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ROBO3_HUMAN
Accessionⁱ	Q96MS0Primary (citable) accession number: Q96MS0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
	Last sequence update:	June 21, 2004
	Last modified:	November 7, 2018
	This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM

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UniProtKB - Q96MS0 (ROBO3_HUMAN)

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Roundabout homolog 3

ROBO3

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

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Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

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Keywords - Technical termⁱ