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UniProtKB - Q96LR5 (UB2E2_HUMAN)
Protein
Ubiquitin-conjugating enzyme E2 E2
Gene
UBE2E2
Organism
Homo sapiens (Human)
Status
Functioni
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.
3 PublicationsCatalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 139 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ISG15 transferase activity Source: HGNC-UCL
- ubiquitin conjugating enzyme activity Source: MGI
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- cellular response to DNA damage stimulus Source: MGI
- ISG15-protein conjugation Source: HGNC-UCL
- positive regulation of G1/S transition of mitotic cell cycle Source: MGI
- protein K11-linked ubiquitination Source: UniProtKB
- protein K48-linked ubiquitination Source: UniProtKB
- protein K63-linked ubiquitination Source: UniProtKB
- protein polyubiquitination Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.3.2.24, 2681 |
PathwayCommonsi | Q96LR5 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q96LR5 |
UniPathwayi | UPA00143 |
Protein family/group databases
MoonDBi | Q96LR5, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2 E2 (EC:2.3.2.231 Publication)Alternative name(s): E2 ubiquitin-conjugating enzyme E2 UbcH8 Ubiquitin carrier protein E2 Ubiquitin-protein ligase E2 |
Gene namesi | Name:UBE2E2 Synonyms:UBCH8 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12478, UBE2E2 |
MIMi | 602163, gene |
neXtProti | NX_Q96LR5 |
VEuPathDBi | HostDB:ENSG00000182247 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 7325 |
OpenTargetsi | ENSG00000182247 |
PharmGKBi | PA37128 |
Miscellaneous databases
Pharosi | Q96LR5, Tbio |
Genetic variation databases
BioMutai | UBE2E2 |
DMDMi | 47606201 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000082472 | 2 – 201 | Ubiquitin-conjugating enzyme E2 E2Add BLAST | 200 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 11 | PhosphoserineCombined sources | 1 | |
Modified residuei | 15 | PhosphoserineCombined sources | 1 | |
Modified residuei | 18 | PhosphoserineCombined sources | 1 | |
Modified residuei | 19 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Autoubiquitinated in vitro.1 Publication
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q96LR5 |
jPOSTi | Q96LR5 |
MassIVEi | Q96LR5 |
MaxQBi | Q96LR5 |
PaxDbi | Q96LR5 |
PeptideAtlasi | Q96LR5 |
PRIDEi | Q96LR5 |
ProteomicsDBi | 77239 |
PTM databases
GlyGeni | Q96LR5, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96LR5 |
PhosphoSitePlusi | Q96LR5 |
Expressioni
Gene expression databases
Bgeei | ENSG00000182247, Expressed in female gonad and 219 other tissues |
ExpressionAtlasi | Q96LR5, baseline and differential |
Genevisiblei | Q96LR5, HS |
Organism-specific databases
HPAi | ENSG00000182247, Low tissue specificity |
Interactioni
Binary interactionsi
Q96LR5
Protein-protein interaction databases
BioGRIDi | 113173, 92 interactors |
DIPi | DIP-52704N |
IntActi | Q96LR5, 54 interactors |
MINTi | Q96LR5 |
STRINGi | 9606.ENSP00000379931 |
Miscellaneous databases
RNActi | Q96LR5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q96LR5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96LR5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 201 | UBC corePROSITE-ProRule annotationAdd BLAST | 147 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 55 | DisorderedSequence analysisAdd BLAST | 55 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 24 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 25 – 47 | Basic and acidic residuesSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0417, Eukaryota |
GeneTreei | ENSGT00940000155985 |
HOGENOMi | CLU_030988_14_4_1 |
InParanoidi | Q96LR5 |
OMAi | LNCDICK |
OrthoDBi | 1337945at2759 |
PhylomeDBi | Q96LR5 |
TreeFami | TF101117 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
IDEALi | IID00640 |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q96LR5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTEAQRVDD SPSTSGGSSD GDQRESVQQE PEREQVQPKK KEGKISSKTA
60 70 80 90 100
AKLSTSAKRI QKELAEITLD PPPNCSAGPK GDNIYEWRST ILGPPGSVYE
110 120 130 140 150
GGVFFLDITF SPDYPFKPPK VTFRTRIYHC NINSQGVICL DILKDNWSPA
160 170 180 190 200
LTISKVLLSI CSLLTDCNPA DPLVGSIATQ YMTNRAEHDR MARQWTKRYA
T
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087X283 | A0A087X283_HUMAN | Ubiquitin-conjugating enzyme E2 E2 | UBE2E2 | 94 | Annotation score: | ||
C9J180 | C9J180_HUMAN | Ubiquitin-conjugating enzyme E2 E2 | UBE2E2 | 164 | Annotation score: | ||
F8WF15 | F8WF15_HUMAN | Ubiquitin-conjugating enzyme E2 E2 | UBE2E2 | 89 | Annotation score: | ||
F8W8F0 | F8W8F0_HUMAN | Ubiquitin-conjugating enzyme E2 E2 | UBE2E2 | 92 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK057886 mRNA Translation: BAB71605.1 BC022332 mRNA Translation: AAH22332.1 |
CCDSi | CCDS2637.1 |
RefSeqi | NP_689866.1, NM_152653.3 XP_005265489.1, XM_005265432.2 |
Genome annotation databases
Ensembli | ENST00000396703; ENSP00000379931; ENSG00000182247 ENST00000425792; ENSP00000401053; ENSG00000182247 |
GeneIDi | 7325 |
KEGGi | hsa:7325 |
MANE-Selecti | ENST00000396703.6; ENSP00000379931.1; NM_152653.4; NP_689866.1 |
UCSCi | uc003ccg.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK057886 mRNA Translation: BAB71605.1 BC022332 mRNA Translation: AAH22332.1 |
CCDSi | CCDS2637.1 |
RefSeqi | NP_689866.1, NM_152653.3 XP_005265489.1, XM_005265432.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Y6L | X-ray | 1.85 | A/B/C | 55-201 | [»] | |
6W9A | X-ray | 2.30 | A/C | 30-201 | [»] | |
SMRi | Q96LR5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113173, 92 interactors |
DIPi | DIP-52704N |
IntActi | Q96LR5, 54 interactors |
MINTi | Q96LR5 |
STRINGi | 9606.ENSP00000379931 |
Protein family/group databases
MoonDBi | Q96LR5, Predicted |
PTM databases
GlyGeni | Q96LR5, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96LR5 |
PhosphoSitePlusi | Q96LR5 |
Genetic variation databases
BioMutai | UBE2E2 |
DMDMi | 47606201 |
Proteomic databases
EPDi | Q96LR5 |
jPOSTi | Q96LR5 |
MassIVEi | Q96LR5 |
MaxQBi | Q96LR5 |
PaxDbi | Q96LR5 |
PeptideAtlasi | Q96LR5 |
PRIDEi | Q96LR5 |
ProteomicsDBi | 77239 |
Protocols and materials databases
Antibodypediai | 11339, 175 antibodies from 31 providers |
CPTCi | Q96LR5, 3 antibodies |
DNASUi | 7325 |
Genome annotation databases
Ensembli | ENST00000396703; ENSP00000379931; ENSG00000182247 ENST00000425792; ENSP00000401053; ENSG00000182247 |
GeneIDi | 7325 |
KEGGi | hsa:7325 |
MANE-Selecti | ENST00000396703.6; ENSP00000379931.1; NM_152653.4; NP_689866.1 |
UCSCi | uc003ccg.3, human |
Organism-specific databases
CTDi | 7325 |
DisGeNETi | 7325 |
GeneCardsi | UBE2E2 |
HGNCi | HGNC:12478, UBE2E2 |
HPAi | ENSG00000182247, Low tissue specificity |
MIMi | 602163, gene |
neXtProti | NX_Q96LR5 |
OpenTargetsi | ENSG00000182247 |
PharmGKBi | PA37128 |
VEuPathDBi | HostDB:ENSG00000182247 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0417, Eukaryota |
GeneTreei | ENSGT00940000155985 |
HOGENOMi | CLU_030988_14_4_1 |
InParanoidi | Q96LR5 |
OMAi | LNCDICK |
OrthoDBi | 1337945at2759 |
PhylomeDBi | Q96LR5 |
TreeFami | TF101117 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
BRENDAi | 2.3.2.24, 2681 |
PathwayCommonsi | Q96LR5 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q96LR5 |
Miscellaneous databases
BioGRID-ORCSi | 7325, 4 hits in 1046 CRISPR screens |
ChiTaRSi | UBE2E2, human |
EvolutionaryTracei | Q96LR5 |
GeneWikii | UBE2E2 |
GenomeRNAii | 7325 |
Pharosi | Q96LR5, Tbio |
PROi | PR:Q96LR5 |
RNActi | Q96LR5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000182247, Expressed in female gonad and 219 other tissues |
ExpressionAtlasi | Q96LR5, baseline and differential |
Genevisiblei | Q96LR5, HS |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
IDEALi | IID00640 |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UB2E2_HUMAN | |
Accessioni | Q96LR5Primary (citable) accession number: Q96LR5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families