UniProtKB - Q96LB2 (MRGX1_HUMAN)
Protein
Mas-related G-protein coupled receptor member X1
Gene
MRGPRX1
Organism
Homo sapiens (Human)
Status
Functioni
Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22)(PubMed:26582731). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. Activated by the antimalarial drug, chloroquine. May mediate chloroquine-induced itch, in a histamine-independent manner.3 Publications
Miscellaneous
Activation of this receptor requires concentrations that exceed the chloroquine concentrations observed in plasma of patients undergoing chloroquine treatment. However, chloroquine accumulates at much higher level in the skin where the receptor is located. Chloroquine-induced itch is very common among black Africans (up to 70%) but less common in other populations.
GO - Molecular functioni
- G protein-coupled receptor activity Source: GO_Central
- transmembrane signaling receptor activity Source: UniProtKB
GO - Biological processi
- acute-phase response Source: UniProtKB-KW
- cell surface receptor signaling pathway Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: GO_Central
- response to chloroquine Source: UniProtKB
- signal transduction Source: UniProtKB
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Biological process | Acute phase |
Enzyme and pathway databases
PathwayCommonsi | Q96LB2 |
SIGNORi | Q96LB2 |
Names & Taxonomyi
Protein namesi | Recommended name: Mas-related G-protein coupled receptor member X1Alternative name(s): Sensory neuron-specific G-protein coupled receptor 3/4 |
Gene namesi | Name:MRGPRX1 Synonyms:MRGX1, SNSR3, SNSR4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000170255.7 |
HGNCi | HGNC:17962, MRGPRX1 |
MIMi | 607227, gene |
neXtProti | NX_Q96LB2 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell surface Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 31 | ExtracellularSequence analysisAdd BLAST | 31 | |
Transmembranei | 32 – 52 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 53 – 67 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 68 – 88 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 89 – 96 | ExtracellularSequence analysis | 8 | |
Transmembranei | 97 – 117 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 118 – 144 | CytoplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 145 – 165 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 166 – 177 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 178 – 198 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 199 – 221 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 222 – 242 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 243 – 254 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 255 – 275 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 276 – 322 | CytoplasmicSequence analysisAdd BLAST | 47 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 259249 |
OpenTargetsi | ENSG00000170255 |
PharmGKBi | PA142671334 |
Miscellaneous databases
Pharosi | Q96LB2, Tchem |
Chemistry databases
ChEMBLi | CHEMBL5850 |
GuidetoPHARMACOLOGYi | 156 |
Polymorphism and mutation databases
BioMutai | MRGPRX1 |
DMDMi | 50401128 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069772 | 1 – 322 | Mas-related G-protein coupled receptor member X1Add BLAST | 322 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 16 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q96LB2 |
PeptideAtlasi | Q96LB2 |
PRIDEi | Q96LB2 |
ProteomicsDBi | 77186 |
PTM databases
GlyGeni | Q96LB2, 1 site |
iPTMneti | Q96LB2 |
PhosphoSitePlusi | Q96LB2 |
Expressioni
Tissue specificityi
Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons.1 Publication
Gene expression databases
Bgeei | ENSG00000170255, Expressed in sural nerve and 13 other tissues |
ExpressionAtlasi | Q96LB2, baseline and differential |
Genevisiblei | Q96LB2, HS |
Organism-specific databases
HPAi | ENSG00000170255, Not detected |
Interactioni
Protein-protein interaction databases
BioGRIDi | 129233, 4 interactors |
STRINGi | 9606.ENSP00000305766 |
Chemistry databases
BindingDBi | Q96LB2 |
Miscellaneous databases
RNActi | Q96LB2, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RTWA, Eukaryota |
GeneTreei | ENSGT01010000222446 |
HOGENOMi | CLU_009579_4_1_1 |
InParanoidi | Q96LB2 |
OMAi | PFGIQFF |
OrthoDBi | 1123658at2759 |
PhylomeDBi | Q96LB2 |
TreeFami | TF336336 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR026234, MRGPCRFAMILY IPR027338, MRGPCRX1 |
PANTHERi | PTHR11334, PTHR11334, 1 hit PTHR11334:SF22, PTHR11334:SF22, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR02108, MRGPCRFAMILY |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q96LB2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDPTISTLDT ELTPINGTEE TLCYKQTLSL TVLTCIVSLV GLTGNAVVLW
60 70 80 90 100
LLGCRMRRNA FSIYILNLAA ADFLFLSGRL IYSLLSFISI PHTISKILYP
110 120 130 140 150
VMMFSYFAGL SFLSAVSTER CLSVLWPIWY RCHRPTHLSA VVCVLLWALS
160 170 180 190 200
LLRSILEWML CGFLFSGADS AWCQTSDFIT VAWLIFLCVV LCGSSLVLLI
210 220 230 240 250
RILCGSRKIP LTRLYVTILL TVLVFLLCGL PFGIQFFLFL WIHVDREVLF
260 270 280 290 300
CHVHLVSIFL SALNSSANPI IYFFVGSFRQ RQNRQNLKLV LQRALQDASE
310 320
VDEGGGQLPE EILELSGSRL EQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A494C1K4 | A0A494C1K4_HUMAN | Mas-related G-protein-coupled recep... | MRGPRX1 | 214 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | I → V in AAL86880 (PubMed:11850634).Curated | 1 | |
Sequence conflicti | 278 | F → L in AAL86880 (PubMed:11850634).Curated | 1 | |
Sequence conflicti | 298 – 299 | AS → TP in AAL86880 (PubMed:11850634).Curated | 2 | |
Sequence conflicti | 307 | Q → W in AAL86880 (PubMed:11850634).Curated | 1 | |
Sequence conflicti | 310 – 312 | EEI → QET in AAL86880 (PubMed:11850634).Curated | 3 | |
Sequence conflicti | 321 | E → D in AAH96835 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_019432 | 36 | I → V Polymorphism; no alteration in ligand-mediated receptor activity. 1 PublicationCorresponds to variant dbSNP:rs11024885Ensembl. | 1 | |
Natural variantiVAR_075462 | 46 | A → T Polymorphism; no alteration in ligand-mediated receptor activity. 1 PublicationCorresponds to variant dbSNP:rs78179510Ensembl. | 1 | |
Natural variantiVAR_075463 | 55 | R → L Polymorphism; no alteration in ligand-mediated receptor activity. 1 PublicationCorresponds to variant dbSNP:rs55954376EnsemblClinVar. | 1 | |
Natural variantiVAR_075464 | 131 | R → S Polymorphism; decrease in ligand-mediated and ligand-independent receptor activity. 1 PublicationCorresponds to variant dbSNP:rs111448117Ensembl. | 1 | |
Natural variantiVAR_075465 | 133 | H → R Polymorphism; increase in ligand-mediated receptor activity. 1 PublicationCorresponds to variant dbSNP:rs140351170Ensembl. | 1 | |
Natural variantiVAR_075466 | 137 | H → R Polymorphism; no alteration in ligand-mediated receptor activity. 1 PublicationCorresponds to variant dbSNP:rs143702818Ensembl. | 1 | |
Natural variantiVAR_075467 | 273 | F → L Polymorphism; no alteration in ligand-mediated receptor activity. 1 PublicationCorresponds to variant dbSNP:rs137866403Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY042213 Genomic DNA Translation: AAK91804.1 AF474989 Genomic DNA Translation: AAL86880.2 AF474990 Genomic DNA Translation: AAL86881.1 AB065846 Genomic DNA Translation: BAC06064.1 AB083628 Genomic DNA Translation: BAB89341.1 AC023078 Genomic DNA No translation available. BC096835 mRNA Translation: AAH96835.1 |
CCDSi | CCDS7846.1 |
RefSeqi | NP_671732.3, NM_147199.3 |
Genome annotation databases
Ensembli | ENST00000302797; ENSP00000305766; ENSG00000170255 |
GeneIDi | 259249 |
KEGGi | hsa:259249 |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY042213 Genomic DNA Translation: AAK91804.1 AF474989 Genomic DNA Translation: AAL86880.2 AF474990 Genomic DNA Translation: AAL86881.1 AB065846 Genomic DNA Translation: BAC06064.1 AB083628 Genomic DNA Translation: BAB89341.1 AC023078 Genomic DNA No translation available. BC096835 mRNA Translation: AAH96835.1 |
CCDSi | CCDS7846.1 |
RefSeqi | NP_671732.3, NM_147199.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 129233, 4 interactors |
STRINGi | 9606.ENSP00000305766 |
Chemistry databases
BindingDBi | Q96LB2 |
ChEMBLi | CHEMBL5850 |
GuidetoPHARMACOLOGYi | 156 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q96LB2, 1 site |
iPTMneti | Q96LB2 |
PhosphoSitePlusi | Q96LB2 |
Polymorphism and mutation databases
BioMutai | MRGPRX1 |
DMDMi | 50401128 |
Proteomic databases
PaxDbi | Q96LB2 |
PeptideAtlasi | Q96LB2 |
PRIDEi | Q96LB2 |
ProteomicsDBi | 77186 |
Protocols and materials databases
Antibodypediai | 25195, 120 antibodies |
Genome annotation databases
Ensembli | ENST00000302797; ENSP00000305766; ENSG00000170255 |
GeneIDi | 259249 |
KEGGi | hsa:259249 |
Organism-specific databases
CTDi | 259249 |
DisGeNETi | 259249 |
EuPathDBi | HostDB:ENSG00000170255.7 |
GeneCardsi | MRGPRX1 |
HGNCi | HGNC:17962, MRGPRX1 |
HPAi | ENSG00000170255, Not detected |
MIMi | 607227, gene |
neXtProti | NX_Q96LB2 |
OpenTargetsi | ENSG00000170255 |
PharmGKBi | PA142671334 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502RTWA, Eukaryota |
GeneTreei | ENSGT01010000222446 |
HOGENOMi | CLU_009579_4_1_1 |
InParanoidi | Q96LB2 |
OMAi | PFGIQFF |
OrthoDBi | 1123658at2759 |
PhylomeDBi | Q96LB2 |
TreeFami | TF336336 |
Enzyme and pathway databases
PathwayCommonsi | Q96LB2 |
SIGNORi | Q96LB2 |
Miscellaneous databases
BioGRID-ORCSi | 259249, 9 hits in 836 CRISPR screens |
GeneWikii | MRGPRX1 |
GenomeRNAii | 259249 |
Pharosi | Q96LB2, Tchem |
PROi | PR:Q96LB2 |
RNActi | Q96LB2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000170255, Expressed in sural nerve and 13 other tissues |
ExpressionAtlasi | Q96LB2, baseline and differential |
Genevisiblei | Q96LB2, HS |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR026234, MRGPCRFAMILY IPR027338, MRGPCRX1 |
PANTHERi | PTHR11334, PTHR11334, 1 hit PTHR11334:SF22, PTHR11334:SF22, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR02108, MRGPCRFAMILY |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MRGX1_HUMAN | |
Accessioni | Q96LB2Primary (citable) accession number: Q96LB2 Secondary accession number(s): Q4V9L2, Q8TDD8, Q8TDD9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries