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Protein

Sorting nexin-27

Gene

SNX27

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.8 Publications

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandLipid-binding

Protein family/group databases

TCDBi9.A.3.1.1 the sorting nexin27 (snx27)-retromer assembly apparatus for recycling integral membrane proteins (snx27-retromeraa) family

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-27
Gene namesi
Name:SNX27
Synonyms:KIAA0488
ORF Names:My014
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000143376.12
HGNCiHGNC:20073 SNX27
MIMi611541 gene
neXtProtiNX_Q96L92

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114H → A: Abolishes interaction with ADRB2, sorting and recycling of ADRB2. 1 Publication1

Organism-specific databases

DisGeNETi81609
MalaCardsiSNX27
OpenTargetsiENSG00000143376
PharmGKBiPA134969143

Polymorphism and mutation databases

BioMutaiSNX27
DMDMi166214988

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003153561 – 541Sorting nexin-27Add BLAST541

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51PhosphoserineCombined sources1
Modified residuei62PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96L92
MaxQBiQ96L92
PaxDbiQ96L92
PeptideAtlasiQ96L92
PRIDEiQ96L92
ProteomicsDBi77161
77162 [Q96L92-2]
77163 [Q96L92-3]

PTM databases

iPTMnetiQ96L92
PhosphoSitePlusiQ96L92

Expressioni

Tissue specificityi

Widely expressed. Expressed in cells of hematopoietic origin (at protein level).3 Publications

Gene expression databases

BgeeiENSG00000143376 Expressed in 243 organ(s), highest expression level in pituitary gland
ExpressionAtlasiQ96L92 baseline and differential
GenevisibleiQ96L92 HS

Organism-specific databases

HPAiHPA045816

Interactioni

Subunit structurei

Core component of the SNX27-retromer, a multiprotein complex composed of SNX27, the WASH complex and the retromer complex. Interacts (via PDZ domain) with a number of target transmembrane proteins (via PDZ-binding motif): ABCC4, ADRB2, ARHGEF7, GRIA1, GRIA2, GRIN1, GRIN2A GRIN2C, KCNJ6, KCNJ9 and SLC2A1/GLUT1. Interacts (via the FERM-like regions) with the WASH complex. Interacts with SNX1. Interacts with CYTIP. Isoform 1 and isoform 2 directly interact with DGKZ. Isoform 1 and isoform 2 interact with HT4R isoform 5-HTA(A). Interacts with MCC.9 Publications

Protein-protein interaction databases

BioGridi123546, 84 interactors
IntActiQ96L92, 14 interactors
MINTiQ96L92
STRINGi9606.ENSP00000357836

Structurei

Secondary structure

1541
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96L92
SMRiQ96L92
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 136PDZPROSITE-ProRule annotationAdd BLAST94
Domaini161 – 269PXPROSITE-ProRule annotationAdd BLAST109
Domaini273 – 362Ras-associatingPROSITE-ProRule annotationAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni273 – 362FERM-like region F1Add BLAST90
Regioni373 – 421FERM-like region F2Add BLAST49
Regioni425 – 525FERM-like region F3Add BLAST101

Domaini

The PDZ domain mediates binding to a subset of proteins containing a PDZ-binding motif at the C-terminus: the specificity for PDZ-binding motif is provided by the 2 residues located upstream of the canonical PDZ-binding motif (By similarity). The PDZ domain also mediates binding to the retromer complex via direct interaction with VPS26 (VPS26A or VPS26B) (PubMed:23563491).By similarity1 Publication
The PX domain mediates binding to phosphatidylinositol 3-phosphate (PtdIns(3P)) and localization to early endosome membranes.1 Publication

Sequence similaritiesi

Belongs to the sorting nexin family.Curated

Phylogenomic databases

eggNOGiENOG410IMRD Eukaryota
ENOG410XPAD LUCA
GeneTreeiENSGT00530000063147
HOVERGENiHBG059206
InParanoidiQ96L92
KOiK17936
OMAiIFPHCSC
OrthoDBiEOG091G147N
PhylomeDBiQ96L92
TreeFamiTF318398

Family and domain databases

CDDicd13338 FERM-like_C_SNX27, 1 hit
cd06886 PX_SNX27, 1 hit
cd01777 SNX27_RA, 1 hit
Gene3Di3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR000159 RA_dom
IPR037831 SNX17/27/31
IPR028667 SNX27
IPR037827 SNX27_FERM-like_dom
IPR037833 SNX27_PX
IPR037835 SNX27_RA
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR12431 PTHR12431, 1 hit
PTHR12431:SF17 PTHR12431:SF17, 1 hit
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
PF00787 PX, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00312 PX, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
SSF54236 SSF54236, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit
PS50195 PX, 1 hit
PS50200 RA, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96L92-1) [UniParc]FASTAAdd to basket
Also known as: SNX27a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADEDGEGIH PSAPHRNGGG GGGGGSGLHC AGNGGGGGGG PRVVRIVKSE
60 70 80 90 100
SGYGFNVRGQ VSEGGQLRSI NGELYAPLQH VSAVLPGGAA DRAGVRKGDR
110 120 130 140 150
ILEVNHVNVE GATHKQVVDL IRAGEKELIL TVLSVPPHEA DNLDPSDDSL
160 170 180 190 200
GQSFYDYTEK QAVPISVPRY KHVEQNGEKF VVYNVYMAGR QLCSKRYREF
210 220 230 240 250
AILHQNLKRE FANFTFPRLP GKWPFSLSEQ QLDARRRGLE EYLEKVCSIR
260 270 280 290 300
VIGESDIMQE FLSESDENYN GVSDVELRVA LPDGTTVTVR VKKNSTTDQV
310 320 330 340 350
YQAIAAKVGM DSTTVNYFAL FEVISHSFVR KLAPNEFPHK LYIQNYTSAV
360 370 380 390 400
PGTCLTIRKW LFTTEEEILL NDNDLAVTYF FHQAVDDVKK GYIKAEEKSY
410 420 430 440 450
QLQKLYEQRK MVMYLNMLRT CEGYNEIIFP HCACDSRRKG HVITAISITH
460 470 480 490 500
FKLHACTEEG QLENQVIAFE WDEMQRWDTD EEGMAFCFEY ARGEKKPRWV
510 520 530 540
KIFTPYFNYM HECFERVFCE LKWRKENIFQ MARSQQRDVA T
Length:541
Mass (Da):61,265
Last modified:January 15, 2008 - v2
Checksum:i1AA2265E463CB8DA
GO
Isoform 2 (identifier: Q96L92-3) [UniParc]FASTAAdd to basket
Also known as: SNX27b

The sequence of this isoform differs from the canonical sequence as follows:
     527-541: NIFQMARSQQRDVAT → EY

Show »
Length:528
Mass (Da):59,811
Checksum:i803D0E93E1B4A658
GO
Isoform 3 (identifier: Q96L92-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-93: Missing.
     94-104: GVRKGDRILEV → MGLSFSLFPLR
     527-541: NIFQMARSQQRDVAT → EY

Note: May be due to intron retention.
Show »
Length:435
Mass (Da):50,880
Checksum:i8F3AD9392ACDDC56
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2R8Y8A7A0A2R8Y8A7_HUMAN
Sorting nexin-27
SNX27
407Annotation score:
A0A2R8Y3S6A0A2R8Y3S6_HUMAN
Sorting nexin-27
SNX27
194Annotation score:
A0A2R8Y871A0A2R8Y871_HUMAN
Sorting nexin-27
SNX27
129Annotation score:
H7C603H7C603_HUMAN
Sorting nexin-27
SNX27
65Annotation score:
A0A2R8YD75A0A2R8YD75_HUMAN
Sorting nexin-27
SNX27
20Annotation score:

Sequence cautioni

The sequence AAG43127 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH12184 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti467I → V in AAI07863 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_059851459E → K. Corresponds to variant dbSNP:rs11204871Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0305371 – 93Missing in isoform 3. 1 PublicationAdd BLAST93
Alternative sequenceiVSP_03053894 – 104GVRKGDRILEV → MGLSFSLFPLR in isoform 3. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_030539527 – 541NIFQM…RDVAT → EY in isoform 2 and isoform 3. 4 PublicationsAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY044866 mRNA Translation: AAK97797.1
AL391335 Genomic DNA No translation available.
AL589765 Genomic DNA No translation available.
BC012184 mRNA Translation: AAH12184.1 Different initiation.
BC051817 mRNA Translation: AAH51817.1
BC071825 mRNA Translation: AAH71825.1
BC100998 mRNA Translation: AAI00999.1
BC100999 mRNA Translation: AAI01000.1
BC101000 mRNA Translation: AAI01001.1
BC101822 mRNA Translation: AAI01823.1
BC101824 mRNA Translation: AAI01825.1
BC107862 mRNA Translation: AAI07863.1
AB007957 mRNA Translation: BAE16986.1
AF060509 mRNA Translation: AAG43127.1 Different initiation.
CCDSiCCDS1001.1 [Q96L92-3]
CCDS81377.1 [Q96L92-1]
RefSeqiNP_001317652.1, NM_001330723.1 [Q96L92-1]
NP_112180.4, NM_030918.5 [Q96L92-3]
UniGeneiHs.192326

Genome annotation databases

EnsembliENST00000368843; ENSP00000357836; ENSG00000143376 [Q96L92-3]
ENST00000458013; ENSP00000400333; ENSG00000143376 [Q96L92-1]
GeneIDi81609
KEGGihsa:81609
UCSCiuc001eyn.2 human [Q96L92-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY044866 mRNA Translation: AAK97797.1
AL391335 Genomic DNA No translation available.
AL589765 Genomic DNA No translation available.
BC012184 mRNA Translation: AAH12184.1 Different initiation.
BC051817 mRNA Translation: AAH51817.1
BC071825 mRNA Translation: AAH71825.1
BC100998 mRNA Translation: AAI00999.1
BC100999 mRNA Translation: AAI01000.1
BC101000 mRNA Translation: AAI01001.1
BC101822 mRNA Translation: AAI01823.1
BC101824 mRNA Translation: AAI01825.1
BC107862 mRNA Translation: AAI07863.1
AB007957 mRNA Translation: BAE16986.1
AF060509 mRNA Translation: AAG43127.1 Different initiation.
CCDSiCCDS1001.1 [Q96L92-3]
CCDS81377.1 [Q96L92-1]
RefSeqiNP_001317652.1, NM_001330723.1 [Q96L92-1]
NP_112180.4, NM_030918.5 [Q96L92-3]
UniGeneiHs.192326

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HASX-ray1.74A/B156-265[»]
ProteinModelPortaliQ96L92
SMRiQ96L92
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123546, 84 interactors
IntActiQ96L92, 14 interactors
MINTiQ96L92
STRINGi9606.ENSP00000357836

Protein family/group databases

TCDBi9.A.3.1.1 the sorting nexin27 (snx27)-retromer assembly apparatus for recycling integral membrane proteins (snx27-retromeraa) family

PTM databases

iPTMnetiQ96L92
PhosphoSitePlusiQ96L92

Polymorphism and mutation databases

BioMutaiSNX27
DMDMi166214988

Proteomic databases

EPDiQ96L92
MaxQBiQ96L92
PaxDbiQ96L92
PeptideAtlasiQ96L92
PRIDEiQ96L92
ProteomicsDBi77161
77162 [Q96L92-2]
77163 [Q96L92-3]

Protocols and materials databases

DNASUi81609
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368843; ENSP00000357836; ENSG00000143376 [Q96L92-3]
ENST00000458013; ENSP00000400333; ENSG00000143376 [Q96L92-1]
GeneIDi81609
KEGGihsa:81609
UCSCiuc001eyn.2 human [Q96L92-1]

Organism-specific databases

CTDi81609
DisGeNETi81609
EuPathDBiHostDB:ENSG00000143376.12
GeneCardsiSNX27
HGNCiHGNC:20073 SNX27
HPAiHPA045816
MalaCardsiSNX27
MIMi611541 gene
neXtProtiNX_Q96L92
OpenTargetsiENSG00000143376
PharmGKBiPA134969143
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IMRD Eukaryota
ENOG410XPAD LUCA
GeneTreeiENSGT00530000063147
HOVERGENiHBG059206
InParanoidiQ96L92
KOiK17936
OMAiIFPHCSC
OrthoDBiEOG091G147N
PhylomeDBiQ96L92
TreeFamiTF318398

Miscellaneous databases

ChiTaRSiSNX27 human
GeneWikiiSNX27
GenomeRNAii81609
PROiPR:Q96L92
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143376 Expressed in 243 organ(s), highest expression level in pituitary gland
ExpressionAtlasiQ96L92 baseline and differential
GenevisibleiQ96L92 HS

Family and domain databases

CDDicd13338 FERM-like_C_SNX27, 1 hit
cd06886 PX_SNX27, 1 hit
cd01777 SNX27_RA, 1 hit
Gene3Di3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR000159 RA_dom
IPR037831 SNX17/27/31
IPR028667 SNX27
IPR037827 SNX27_FERM-like_dom
IPR037833 SNX27_PX
IPR037835 SNX27_RA
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR12431 PTHR12431, 1 hit
PTHR12431:SF17 PTHR12431:SF17, 1 hit
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
PF00787 PX, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00312 PX, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
SSF54236 SSF54236, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit
PS50195 PX, 1 hit
PS50200 RA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSNX27_HUMAN
AccessioniPrimary (citable) accession number: Q96L92
Secondary accession number(s): Q32Q36
, Q4AEJ5, Q5VWB0, Q5VWB1, Q5VWB2, Q6IPP6, Q86UB1, Q96D79, Q9H3K8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: January 15, 2008
Last modified: October 10, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  6. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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