UniProtKB - Q96L34 (MARK4_HUMAN)

BLAST

>sp|Q96L34|MARK4_HUMAN MAP/microtubule affinity-regulating kinase 4 OS=Homo sapiens OX=9606 GN=MARK4 PE=1 SV=1
MSSRTVLAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYR
LLRTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVK
LFEVIETEKTLYLVMEYASAGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHR
DLKAENLLLDAEANIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDIW
SLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCT
LEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKESLTSQKYNEV
TATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGTSHSKGQRSSSSTYHRQR
RHSDFCGPSPAPLHPKRSPTSTGEAELKEERLPGRKASCSTAGSGSRGLPPSSPMVSSAH
NPNKAEIPERRKDSTSTPNNLPPSMMTRRNTYVCTERPGAERPSLLPNGKENSSGTPRVP
PASPSSHSLAPPSGERSRLARGSTIRSTFHGGQVRDRRAGGGGGGGVQNGPPASPTLAHE
AAPLPAGRPRPTTNLFTKLTSKLTRRVADEPERIGGPEVTSCHLPWDQTETAPRLLRFPW
SVKLTSSRPPEALMAALRQATAAARCRCRQPQPFLLACLHGGAGGPEPLSHFEVEVCQLP
RPGLRGVLFRRVAGTALAFRTLVTRISNDLEL

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History
Entry version 173 (07 Nov 2018)
Sequence version 1 (01 Dec 2001)
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Protein

MAP/microtubule affinity-regulating kinase 4

Gene

MARK4

Organism

Homo sapiens (Human)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942). Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942).By similarity6 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.3 Publications

Cofactorⁱ

Mg²⁺1 Publication

Activity regulationⁱ

Activated by phosphorylation on Thr-214.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	88	ATPPROSITE-ProRule annotation		1
Active siteⁱ	181	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	65 – 73	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

ATP binding
Source: UniProtKB
Non-traceable author statementⁱ
- 15009667
gamma-tubulin binding
Source: UniProtKB
Inferred from direct assayⁱ
- 14594945
microtubule binding
Source: UniProtKB
Inferred from direct assayⁱ
- 14594945
protein serine/threonine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 15009667
tau-protein kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 14594945
ubiquitin binding
Source: UniProtKB
Non-traceable author statementⁱ
- 14594945

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
cell division Source: UniProtKB-KW
ciliary basal body-plasma membrane docking Source: Reactome
cilium organization
Source: ARUK-UCL
Inferred from genetic interactionⁱ
- 23400999
establishment of cell polarity Source: InterPro
intracellular signal transduction
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
microtubule bundle formation
Source: UniProtKB
Inferred from expression patternⁱ
- 14594945
microtubule cytoskeleton organization
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 14594945
nervous system development
Source: UniProtKB
Inferred from direct assayⁱ
- 14594945
positive regulation of cilium assembly
Source: ARUK-UCL
Inferred from mutant phenotypeⁱ
- 23400999
positive regulation of programmed cell death
Source: UniProtKB
Non-traceable author statementⁱ
- 15009667
positive regulation of protein localization to centrosome
Source: ARUK-UCL
Inferred from mutant phenotypeⁱ
- 23400999
protein phosphorylation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23666762
regulation of centrosome cycle
Source: ARUK-UCL
Inferred from mutant phenotypeⁱ
- 25123532

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Cell cycle, Cell division, Cilium biogenesis/degradation, Mitosis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-HSA-5620912 Anchoring of the basal body to the plasma membrane
SignaLinkⁱ	Q96L34
SIGNORⁱ	Q96L34

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: MAP/microtubule affinity-regulating kinase 4 (EC:2.7.11.13 Publications) Alternative name(s): MAP/microtubule affinity-regulating kinase-like 1
Gene namesⁱ	Name:MARK4 Synonyms:KIAA1860, MARKL1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 19

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000007047.14
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:13538 MARK4
MIMⁱ	606495 gene
neXtProtⁱ	NX_Q96L34

Keywords - Cellular componentⁱ

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	214	T → A: Prevents phosphorylation and activation by STK11/LKB1 complex. 1 Publication		1
Mutagenesisⁱ	214	T → E: Mimicks phosphorylation state, leading to increased activity. Decreases mTORC1 activity. 2 Publications		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	57787
Open Targets More...OpenTargetsⁱ	ENSG00000007047
PharmGKBⁱ	PA30641

Chemistry databases

ChEMBLⁱ	CHEMBL5754

ChEMBLⁱ

CHEMBL5754

Polymorphism and mutation databases

BioMutaⁱ	MARK4
DMDMⁱ	29840797

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000086307	1 – 752	MAP/microtubule affinity-regulating kinase 4Add BLAST		752

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	214	Phosphothreonine; by LKB11 Publication	1
Modified residueⁱ	423	PhosphoserineBy similarity	1
Modified residueⁱ	543	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.2 Publications

Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).2 Publications

Keywords - PTMⁱ

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q96L34
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q96L34
PaxDbⁱ	Q96L34
PeptideAtlas More...PeptideAtlasⁱ	Q96L34
PRIDEⁱ	Q96L34
ProteomicsDBⁱ	77145 77146 [Q96L34-2]

PTM databases

iPTMnetⁱ	Q96L34
PhosphoSitePlusⁱ	Q96L34

Expressionⁱ

Tissue specificityⁱ

Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).2 Publications

Developmental stageⁱ

Expressed at all stages of the mitotic cell cycle.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000007047 Expressed in 225 organ(s), highest expression level in male germ cell
CleanExⁱ	HS_MARK4
ExpressionAtlasⁱ	Q96L34 baseline and differential
Genevisibleⁱ	Q96L34 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA039186

HPAⁱ

HPA039186

Interactionⁱ

Subunit structureⁱ

Interacts with MAPT/TAU (PubMed:23666762). Interacts with gamma-tubulin (PubMed:14594945). Interacts with ODF2 (PubMed:23400999). Interacts with USP9X (PubMed:18254724).4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CDC42	P60953	2	EBI-302319,EBI-81752
PARD6G	Q9BYG4	2	EBI-302319,EBI-295417
PNMA1	Q8ND90	2	EBI-302319,EBI-302345
PRKCI	P41743	2	EBI-302319,EBI-286199
STK11	Q15831	2	EBI-302319,EBI-306838
TUBG1	P23258	4	EBI-302319,EBI-302589
YWHAH	Q04917	6	EBI-302319,EBI-306940

GO - Molecular functionⁱ

gamma-tubulin binding
Source: UniProtKB
Inferred from direct assayⁱ
- 14594945
microtubule binding
Source: UniProtKB
Inferred from direct assayⁱ
- 14594945
ubiquitin binding
Source: UniProtKB
Non-traceable author statementⁱ
- 14594945

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	121760, 51 interactors
IntActⁱ	Q96L34, 29 interactors
STRINGⁱ	9606.ENSP00000262891

Chemistry databases

BindingDBⁱ

Q96L34

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	59 – 67	Combined sources	9
Beta strandⁱ	69 – 78	Combined sources	10
Turnⁱ	79 – 81	Combined sources	3
Beta strandⁱ	84 – 91	Combined sources	8
Helixⁱ	92 – 94	Combined sources	3
Helixⁱ	97 – 111	Combined sources	15
Beta strandⁱ	121 – 126	Combined sources	6
Beta strandⁱ	128 – 135	Combined sources	8
Helixⁱ	143 – 150	Combined sources	8
Helixⁱ	155 – 174	Combined sources	20
Beta strandⁱ	186 – 189	Combined sources	4
Beta strandⁱ	195 – 197	Combined sources	3
Turnⁱ	219 – 221	Combined sources	3
Helixⁱ	224 – 228	Combined sources	5
Helixⁱ	235 – 251	Combined sources	17
Helixⁱ	261 – 270	Combined sources	10
Helixⁱ	281 – 290	Combined sources	10
Turnⁱ	295 – 297	Combined sources	3
Helixⁱ	301 – 304	Combined sources	4
Helixⁱ	308 – 311	Combined sources	4
Helixⁱ	332 – 339	Combined sources	8
Turnⁱ	340 – 342	Combined sources	3
Helixⁱ	345 – 352	Combined sources	8
Turnⁱ	353 – 355	Combined sources	3
Helixⁱ	359 – 366	Combined sources	8
Turnⁱ	367 – 369	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	Q96L34
SMRⁱ	Q96L34
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	59 – 310	Protein kinasePROSITE-ProRule annotationAdd BLAST	252
Domainⁱ	324 – 368	UBAPROSITE-ProRule annotationAdd BLAST	45
Domainⁱ	703 – 752	KA1PROSITE-ProRule annotationAdd BLAST	50

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG0586 Eukaryota ENOG410XNQ0 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140806
HOGENOMⁱ	HOG000233025
HOVERGENⁱ	HBG052453
InParanoidⁱ	Q96L34
KEGG Orthology (KO) More...KOⁱ	K08798
OMAⁱ	FCGPSPT
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0D1E
PhylomeDBⁱ	Q96L34
TreeFamⁱ	TF315213

Family and domain databases

InterProⁱ	View protein in InterPro IPR028375 KA1/Ssp2_C IPR001772 KA1_dom IPR011009 Kinase-like_dom_sf IPR033624 MARK/par1 IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS IPR015940 UBA
PANTHERⁱ	PTHR24346 PTHR24346, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02149 KA1, 1 hit PF00069 Pkinase, 1 hit PF00627 UBA, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit SM00165 UBA, 1 hit
SUPFAMⁱ	SSF103243 SSF103243, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS50032 KA1, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit PS50030 UBA, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q96L34-1) [UniParc]FASTA Add to basket

Also known as: MARK4L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSRTVLAPG NDRNSDTHGT LGSGRSSDKG PSWSSRSLGA RCRNSIASCP 
        60         70         80         90        100
EEQPHVGNYR LLRTIGKGNF AKVKLARHIL TGREVAIKII DKTQLNPSSL 
       110        120        130        140        150
QKLFREVRIM KGLNHPNIVK LFEVIETEKT LYLVMEYASA GEVFDYLVSH 
       160        170        180        190        200
GRMKEKEARA KFRQIVSAVH YCHQKNIVHR DLKAENLLLD AEANIKIADF 
       210        220        230        240        250
GFSNEFTLGS KLDTFCGSPP YAAPELFQGK KYDGPEVDIW SLGVILYTLV 
       260        270        280        290        300
SGSLPFDGHN LKELRERVLR GKYRVPFYMS TDCESILRRF LVLNPAKRCT 
       310        320        330        340        350
LEQIMKDKWI NIGYEGEELK PYTEPEEDFG DTKRIEVMVG MGYTREEIKE 
       360        370        380        390        400
SLTSQKYNEV TATYLLLGRK TEEGGDRGAP GLALARVRAP SDTTNGTSSS 
       410        420        430        440        450
KGTSHSKGQR SSSSTYHRQR RHSDFCGPSP APLHPKRSPT STGEAELKEE 
       460        470        480        490        500
RLPGRKASCS TAGSGSRGLP PSSPMVSSAH NPNKAEIPER RKDSTSTPNN 
       510        520        530        540        550
LPPSMMTRRN TYVCTERPGA ERPSLLPNGK ENSSGTPRVP PASPSSHSLA 
       560        570        580        590        600
PPSGERSRLA RGSTIRSTFH GGQVRDRRAG GGGGGGVQNG PPASPTLAHE 
       610        620        630        640        650
AAPLPAGRPR PTTNLFTKLT SKLTRRVADE PERIGGPEVT SCHLPWDQTE 
       660        670        680        690        700
TAPRLLRFPW SVKLTSSRPP EALMAALRQA TAAARCRCRQ PQPFLLACLH 
       710        720        730        740        750
GGAGGPEPLS HFEVEVCQLP RPGLRGVLFR RVAGTALAFR TLVTRISNDL 

EL

Length:752

Mass (Da):82,520

Last modified:December 1, 2001 - v1

Checksum:ⁱ4B430FFD2B150E7A

Isoform 2 (identifier: Q96L34-2) [UniParc]FASTA Add to basket

Also known as: MARKL1S, MARK4S

The sequence of this isoform differs from the canonical sequence as follows:
628-752: ADEPERIGGP...VTRISNDLEL → TLDPSKRQNS...PPGCSDSPGV

« Hide

        10         20         30         40         50
MSSRTVLAPG NDRNSDTHGT LGSGRSSDKG PSWSSRSLGA RCRNSIASCP 
        60         70         80         90        100
EEQPHVGNYR LLRTIGKGNF AKVKLARHIL TGREVAIKII DKTQLNPSSL 
       110        120        130        140        150
QKLFREVRIM KGLNHPNIVK LFEVIETEKT LYLVMEYASA GEVFDYLVSH 
       160        170        180        190        200
GRMKEKEARA KFRQIVSAVH YCHQKNIVHR DLKAENLLLD AEANIKIADF 
       210        220        230        240        250
GFSNEFTLGS KLDTFCGSPP YAAPELFQGK KYDGPEVDIW SLGVILYTLV 
       260        270        280        290        300
SGSLPFDGHN LKELRERVLR GKYRVPFYMS TDCESILRRF LVLNPAKRCT 
       310        320        330        340        350
LEQIMKDKWI NIGYEGEELK PYTEPEEDFG DTKRIEVMVG MGYTREEIKE 
       360        370        380        390        400
SLTSQKYNEV TATYLLLGRK TEEGGDRGAP GLALARVRAP SDTTNGTSSS 
       410        420        430        440        450
KGTSHSKGQR SSSSTYHRQR RHSDFCGPSP APLHPKRSPT STGEAELKEE 
       460        470        480        490        500
RLPGRKASCS TAGSGSRGLP PSSPMVSSAH NPNKAEIPER RKDSTSTPNN 
       510        520        530        540        550
LPPSMMTRRN TYVCTERPGA ERPSLLPNGK ENSSGTPRVP PASPSSHSLA 
       560        570        580        590        600
PPSGERSRLA RGSTIRSTFH GGQVRDRRAG GGGGGGVQNG PPASPTLAHE 
       610        620        630        640        650
AAPLPAGRPR PTTNLFTKLT SKLTRRVTLD PSKRQNSNRC VSGASLPQGS 
       660        670        680 
KIRSQTNLRE SGDLRSQVAI YLGIKRKPPP GCSDSPGV

Show »

Length:688

Mass (Da):75,321

Checksum:ⁱA49143B34FDDD086

Computationally mapped potential isoform sequencesⁱ

There are 5 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

Q96GZ3	Q96GZ3_HUMAN	MAP/microtubule affinity-regulating... MAP/microtubule affinity-regulating kinase 4 (MARK4 protein)	MARK4	79	Annotation score:
Q6IPE9	Q6IPE9_HUMAN	MAP/microtubule affinity-regulating... MAP/microtubule affinity-regulating kinase 4 (MARK4 protein)	MARK4	97	Annotation score:
K7EKG8	K7EKG8_HUMAN	MAP/microtubule affinity-regulating... MAP/microtubule affinity-regulating kinase 4	MARK4	74	Annotation score:
K7EK17	K7EK17_HUMAN	MAP/microtubule affinity-regulating... MAP/microtubule affinity-regulating kinase 4	MARK4	62	Annotation score:
K7EN95	K7EN95_HUMAN	MAP/microtubule affinity-regulating... MAP/microtubule affinity-regulating kinase 4	MARK4	48	Annotation score:

Sequence cautionⁱ

The sequence BAB47489 differs from that shown. Reason: Erroneous initiation.Curated

The sequence BAB55238 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	70	F → S in BAB39380 (PubMed:11326310).Curated	1
Sequence conflictⁱ	70	F → S in BAC03375 (PubMed:11326310).Curated	1
Sequence conflictⁱ	518	P → L in BAB55238 (PubMed:14702039).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_040766	377	R → Q1 PublicationCorresponds to variant dbSNP:rs35070611Ensembl.		1
Natural variantⁱVAR_040767	418	R → C in a colorectal adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs780763668Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_004946	628 – 752	ADEPE…NDLEL → TLDPSKRQNSNRCVSGASLP QGSKIRSQTNLRESGDLRSQ VAIYLGIKRKPPPGCSDSPG V in isoform 2. 2 PublicationsAdd BLAST		125

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB049127 mRNA Translation: BAB39380.1 AB088047 mRNA Translation: BAC03375.1 AY057448 mRNA Translation: AAL23683.1 AX305105 mRNA No translation available. AX305106 mRNA No translation available. AY120867 mRNA Translation: AAM55491.1 AB058763 mRNA Translation: BAB47489.1 Different initiation. AK027619 mRNA Translation: BAB55238.1 Different initiation. AK075272 mRNA Translation: BAC11510.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS12658.1 [Q96L34-2] CCDS56097.1 [Q96L34-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001186796.1, NM_001199867.1 [Q96L34-1] NP_113605.2, NM_031417.3 [Q96L34-2]
UniGeneⁱ	Hs.34314

Genome annotation databases

Ensemblⁱ	ENST00000262891; ENSP00000262891; ENSG00000007047 [Q96L34-1] ENST00000300843; ENSP00000300843; ENSG00000007047 [Q96L34-2]
GeneIDⁱ	57787
KEGGⁱ	hsa:57787
UCSC genome browser More...UCSCⁱ	uc002pba.3 human [Q96L34-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q96L34	MAP/microtubule affinity-regulating kinase 4		MOUSE		752	UniRef90_Q96L34
Non-specific serine/threonine protein kinase		ICTTR		916
Non-specific serine/threonine protein kinase		MACNE		803
Non-specific serine/threonine protein kinase		MACMU		768
Non-specific serine/threonine protein kinase		MACFA		768
+111
Q96L34-2	Uncharacterized protein Nbla00650 (Fragment)		HUMAN		381	UniRef90_Q96L34-2
MAP/microtubule affinity-regulating kinase 4 isoform 2		CALJA		688
Uncharacterized protein		Cebus capucinus imitator		688
Uncharacterized protein		AOTNA		688
Uncharacterized protein		MUSPF		688
+72

Protein	Similar proteins		Species	Score	Length	Source
Q96L34	MAP/microtubule affinity-regulating kinase 4		MOUSE		752	UniRef50_Q96L34
Non-specific serine/threonine protein kinase		ICTTR		916
Non-specific serine/threonine protein kinase		MACNE		803
Non-specific serine/threonine protein kinase		MACFA		768
Non-specific serine/threonine protein kinase		MACMU		768
+141
Q96L34-2	Uncharacterized protein Nbla00650 (Fragment)		HUMAN		381	UniRef50_Q96L34-2
MAP/microtubule affinity-regulating kinase 4 isoform X2		DELLE		688
MAP/microtubule affinity-regulating kinase 4 isoform 2		CALJA		688
Microtubule affinity-regulating kinase 4		RAT		688
Uncharacterized protein		MUSPF		688
+296

Cross-referencesⁱ

Web resourcesⁱ

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB049127 mRNA Translation: BAB39380.1 AB088047 mRNA Translation: BAC03375.1 AY057448 mRNA Translation: AAL23683.1 AX305105 mRNA No translation available. AX305106 mRNA No translation available. AY120867 mRNA Translation: AAM55491.1 AB058763 mRNA Translation: BAB47489.1 Different initiation. AK027619 mRNA Translation: BAB55238.1 Different initiation. AK075272 mRNA Translation: BAC11510.1
CCDSⁱ	CCDS12658.1 [Q96L34-2] CCDS56097.1 [Q96L34-1]
RefSeqⁱ	NP_001186796.1, NM_001199867.1 [Q96L34-1] NP_113605.2, NM_031417.3 [Q96L34-2]
UniGeneⁱ	Hs.34314

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5ES1	X-ray	2.80	A	44-370	[»]
ProteinModelPortalⁱ	Q96L34
SMRⁱ	Q96L34
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	121760, 51 interactors
IntActⁱ	Q96L34, 29 interactors
STRINGⁱ	9606.ENSP00000262891

Chemistry databases

BindingDBⁱ	Q96L34
ChEMBLⁱ	CHEMBL5754

PTM databases

iPTMnetⁱ	Q96L34
PhosphoSitePlusⁱ	Q96L34

Polymorphism and mutation databases

BioMutaⁱ	MARK4
DMDMⁱ	29840797

Proteomic databases

EPDⁱ	Q96L34
MaxQBⁱ	Q96L34
PaxDbⁱ	Q96L34
PeptideAtlasⁱ	Q96L34
PRIDEⁱ	Q96L34
ProteomicsDBⁱ	77145 77146 [Q96L34-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	57787
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000262891; ENSP00000262891; ENSG00000007047 [Q96L34-1] ENST00000300843; ENSP00000300843; ENSG00000007047 [Q96L34-2]
GeneIDⁱ	57787
KEGGⁱ	hsa:57787
UCSCⁱ	uc002pba.3 human [Q96L34-1]

Organism-specific databases

CTDⁱ	57787
DisGeNETⁱ	57787
EuPathDBⁱ	HostDB:ENSG00000007047.14
GeneCardsⁱ	MARK4
HGNCⁱ	HGNC:13538 MARK4
HPAⁱ	HPA039186
MIMⁱ	606495 gene
neXtProtⁱ	NX_Q96L34
OpenTargetsⁱ	ENSG00000007047
PharmGKBⁱ	PA30641
HUGEⁱ	Search...
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0586 Eukaryota ENOG410XNQ0 LUCA
GeneTreeⁱ	ENSGT00900000140806
HOGENOMⁱ	HOG000233025
HOVERGENⁱ	HBG052453
InParanoidⁱ	Q96L34
KOⁱ	K08798
OMAⁱ	FCGPSPT
OrthoDBⁱ	EOG091G0D1E
PhylomeDBⁱ	Q96L34
TreeFamⁱ	TF315213

Enzyme and pathway databases

Reactomeⁱ	R-HSA-5620912 Anchoring of the basal body to the plasma membrane
SignaLinkⁱ	Q96L34
SIGNORⁱ	Q96L34

Miscellaneous databases

ChiTaRSⁱ	MARK4 human
GeneWikiⁱ	MARK4
GenomeRNAiⁱ	57787
Protein Ontology More...PROⁱ	PR:Q96L34
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000007047 Expressed in 225 organ(s), highest expression level in male germ cell
CleanExⁱ	HS_MARK4
ExpressionAtlasⁱ	Q96L34 baseline and differential
Genevisibleⁱ	Q96L34 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR028375 KA1/Ssp2_C IPR001772 KA1_dom IPR011009 Kinase-like_dom_sf IPR033624 MARK/par1 IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS IPR015940 UBA
PANTHERⁱ	PTHR24346 PTHR24346, 1 hit
Pfamⁱ	View protein in Pfam PF02149 KA1, 1 hit PF00069 Pkinase, 1 hit PF00627 UBA, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit SM00165 UBA, 1 hit
SUPFAMⁱ	SSF103243 SSF103243, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS50032 KA1, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit PS50030 UBA, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MARK4_HUMAN
Accessionⁱ	Q96L34Primary (citable) accession number: Q96L34 Secondary accession number(s): Q8NG37 , Q96JG7, Q96SQ2, Q9BYD8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
	Last sequence update:	December 1, 2001
	Last modified:	November 7, 2018
	This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human and mouse protein kinases
Human and mouse protein kinases: classification and index

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UniProtKB - Q96L34 (MARK4_HUMAN)

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MAP/microtubule affinity-regulating kinase 4

MARK4

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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Keywords - PTMi

Proteomic databases

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Keywords - Coding sequence diversityi

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Chemistry databases

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Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ