UniProtKB - Q96L12 (CALR3_HUMAN)
Protein
Calreticulin-3
Gene
CALR3
Organism
Homo sapiens (Human)
Status
Functioni
During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility (By similarity). CALR3 capacity for calcium-binding may be absent or much lower than that of CALR.By similarity1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 109 | CarbohydrateBy similarity | 1 | |
Binding sitei | 111 | CarbohydrateBy similarity | 1 | |
Binding sitei | 128 | CarbohydrateBy similarity | 1 | |
Binding sitei | 135 | CarbohydrateBy similarity | 1 | |
Binding sitei | 303 | CarbohydrateBy similarity | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein folding chaperone Source: Ensembl
- unfolded protein binding Source: InterPro
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- endoplasmic reticulum unfolded protein response Source: GO_Central
- protein folding Source: GO_Central
- spermatogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Chaperone |
Biological process | Differentiation, Spermatogenesis |
Ligand | Lectin, Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q96L12 |
Names & Taxonomyi
Protein namesi | Recommended name: Calreticulin-3Alternative name(s): Calreticulin-2 Calsperin |
Gene namesi | Name:CALR3 Synonyms:CRT2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20407, CALR3 |
MIMi | 611414, gene |
neXtProti | NX_Q96L12 |
VEuPathDBi | HostDB:ENSG00000269058.5 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen PROSITE-ProRule annotation1 Publication
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: UniProtKB
- endoplasmic reticulum membrane Source: GO_Central
Nucleus
- nuclear envelope Source: UniProtKB
Keywords - Cellular componenti
Endoplasmic reticulumPathology & Biotechi
Keywords - Diseasei
Cardiomyopathy, Disease variantOrganism-specific databases
DisGeNETi | 125972 |
OpenTargetsi | ENSG00000269058 |
Orphaneti | 155, NON RARE IN EUROPE: Familial isolated hypertrophic cardiomyopathy |
PharmGKBi | PA134922944 |
Miscellaneous databases
Pharosi | Q96L12, Tbio |
Chemistry databases
DrugBanki | DB11093, Calcium citrate DB11348, Calcium Phosphate DB14481, Calcium phosphate dihydrate |
Genetic variation databases
BioMutai | CALR3 |
DMDMi | 116241279 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000004178 | 20 – 384 | Calreticulin-3Add BLAST | 365 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 42 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 105 ↔ 137 | By similarity | ||
Glycosylationi | 201 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q96L12 |
MassIVEi | Q96L12 |
PaxDbi | Q96L12 |
PeptideAtlasi | Q96L12 |
PRIDEi | Q96L12 |
ProteomicsDBi | 77137 |
PTM databases
GlyGeni | Q96L12, 2 sites |
iPTMneti | Q96L12 |
PhosphoSitePlusi | Q96L12 |
Expressioni
Tissue specificityi
Testis specific.1 Publication
Gene expression databases
Bgeei | ENSG00000269058, Expressed in testis and 46 other tissues |
ExpressionAtlasi | Q96L12, baseline and differential |
Genevisiblei | Q96L12, HS |
Organism-specific databases
HPAi | ENSG00000269058, Tissue enriched (testis) |
Interactioni
Subunit structurei
Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.
By similarityGO - Molecular functioni
- unfolded protein binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 125941, 65 interactors |
IntActi | Q96L12, 8 interactors |
STRINGi | 9606.ENSP00000269881 |
Miscellaneous databases
RNActi | Q96L12, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 191 – 202 | 1-1Add BLAST | 12 | |
Repeati | 208 – 219 | 1-2Add BLAST | 12 | |
Repeati | 221 – 230 | 1-3 | 10 | |
Repeati | 234 – 245 | 1-4Add BLAST | 12 | |
Repeati | 249 – 259 | 2-1Add BLAST | 11 | |
Repeati | 263 – 271 | 2-2 | 9 | |
Repeati | 273 – 283 | 2-3Add BLAST | 11 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 20 – 197 | N-domainAdd BLAST | 178 | |
Regioni | 191 – 245 | 4 X approximate repeatsAdd BLAST | 55 | |
Regioni | 198 – 294 | P-domainAdd BLAST | 97 | |
Regioni | 249 – 283 | 3 X approximate repeatsAdd BLAST | 35 | |
Regioni | 295 – 384 | C-domainAdd BLAST | 90 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 381 – 384 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 337 – 362 | Glu/Lys-richAdd BLAST | 26 |
Domaini
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity
Sequence similaritiesi
Belongs to the calreticulin family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG0674, Eukaryota |
GeneTreei | ENSGT00950000182915 |
HOGENOMi | CLU_018224_0_0_1 |
InParanoidi | Q96L12 |
OMAi | DWPAPML |
OrthoDBi | 822188at2759 |
PhylomeDBi | Q96L12 |
TreeFami | TF338438 |
Family and domain databases
Gene3Di | 2.10.250.10, 1 hit |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009169, Calreticulin IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 2 hits |
PIRSFi | PIRSF002356, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00014, ER_TARGET, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q96L12-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARALVQLWA ICMLRVALAT VYFQEEFLDG EHWRNRWLQS TNDSRFGHFR
60 70 80 90 100
LSSGKFYGHK EKDKGLQTTQ NGRFYAISAR FKPFSNKGKT LVIQYTVKHE
110 120 130 140 150
QKMDCGGGYI KVFPADIDQK NLNGKSQYYI MFGPDICGFD IKKVHVILHF
160 170 180 190 200
KNKYHENKKL IRCKVDGFTH LYTLILRPDL SYDVKIDGQS IESGSIEYDW
210 220 230 240 250
NLTSLKKETS PAESKDWEQT KDNKAQDWEK HFLDASTSKQ SDWNGDLDGD
260 270 280 290 300
WPAPMLQKPP YQDGLKPEGI HKDVWLHRKM KNTDYLTQYD LSEFENIGAI
310 320 330 340 350
GLELWQVRSG TIFDNFLITD DEEYADNFGK ATWGETKGPE REMDAIQAKE
360 370 380
EMKKAREEEE EELLSGKINR HEHYFNQFHR RNEL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM0R0Y8 | M0R0Y8_HUMAN | Calreticulin-3 | CALR3 | 142 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027944 | 8 | L → F1 PublicationCorresponds to variant dbSNP:rs11544148Ensembl. | 1 | |
Natural variantiVAR_065476 | 82 | K → R Found in a patient with hypertrophic cardiomyopathy; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs142951029EnsemblClinVar. | 1 | |
Natural variantiVAR_027945 | 248 | D → G. Corresponds to variant dbSNP:rs10411092Ensembl. | 1 | |
Natural variantiVAR_027946 | 274 | V → I. Corresponds to variant dbSNP:rs12459238EnsemblClinVar. | 1 | |
Natural variantiVAR_048589 | 284 | D → N. Corresponds to variant dbSNP:rs10404156EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB576176 mRNA Translation: BAJ14705.1 AK058084 mRNA Translation: BAB71655.1 AC008764 Genomic DNA No translation available. CH471106 Genomic DNA Translation: EAW84546.1 BC014595 mRNA Translation: AAH14595.1 |
CCDSi | CCDS12344.1 |
RefSeqi | NP_659483.2, NM_145046.4 |
Genome annotation databases
Ensembli | ENST00000269881; ENSP00000269881; ENSG00000269058 |
GeneIDi | 125972 |
KEGGi | hsa:125972 |
UCSCi | uc002ned.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB576176 mRNA Translation: BAJ14705.1 AK058084 mRNA Translation: BAB71655.1 AC008764 Genomic DNA No translation available. CH471106 Genomic DNA Translation: EAW84546.1 BC014595 mRNA Translation: AAH14595.1 |
CCDSi | CCDS12344.1 |
RefSeqi | NP_659483.2, NM_145046.4 |
3D structure databases
SMRi | Q96L12 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 125941, 65 interactors |
IntActi | Q96L12, 8 interactors |
STRINGi | 9606.ENSP00000269881 |
Chemistry databases
DrugBanki | DB11093, Calcium citrate DB11348, Calcium Phosphate DB14481, Calcium phosphate dihydrate |
PTM databases
GlyGeni | Q96L12, 2 sites |
iPTMneti | Q96L12 |
PhosphoSitePlusi | Q96L12 |
Genetic variation databases
BioMutai | CALR3 |
DMDMi | 116241279 |
Proteomic databases
EPDi | Q96L12 |
MassIVEi | Q96L12 |
PaxDbi | Q96L12 |
PeptideAtlasi | Q96L12 |
PRIDEi | Q96L12 |
ProteomicsDBi | 77137 |
Protocols and materials databases
Antibodypediai | 66699, 311 antibodies |
DNASUi | 125972 |
Genome annotation databases
Ensembli | ENST00000269881; ENSP00000269881; ENSG00000269058 |
GeneIDi | 125972 |
KEGGi | hsa:125972 |
UCSCi | uc002ned.3, human |
Organism-specific databases
CTDi | 125972 |
DisGeNETi | 125972 |
GeneCardsi | CALR3 |
HGNCi | HGNC:20407, CALR3 |
HPAi | ENSG00000269058, Tissue enriched (testis) |
MIMi | 611414, gene |
neXtProti | NX_Q96L12 |
OpenTargetsi | ENSG00000269058 |
Orphaneti | 155, NON RARE IN EUROPE: Familial isolated hypertrophic cardiomyopathy |
PharmGKBi | PA134922944 |
VEuPathDBi | HostDB:ENSG00000269058.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0674, Eukaryota |
GeneTreei | ENSGT00950000182915 |
HOGENOMi | CLU_018224_0_0_1 |
InParanoidi | Q96L12 |
OMAi | DWPAPML |
OrthoDBi | 822188at2759 |
PhylomeDBi | Q96L12 |
TreeFami | TF338438 |
Enzyme and pathway databases
PathwayCommonsi | Q96L12 |
Miscellaneous databases
BioGRID-ORCSi | 125972, 12 hits in 874 CRISPR screens |
GenomeRNAii | 125972 |
Pharosi | Q96L12, Tbio |
PROi | PR:Q96L12 |
RNActi | Q96L12, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000269058, Expressed in testis and 46 other tissues |
ExpressionAtlasi | Q96L12, baseline and differential |
Genevisiblei | Q96L12, HS |
Family and domain databases
Gene3Di | 2.10.250.10, 1 hit |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009169, Calreticulin IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 2 hits |
PIRSFi | PIRSF002356, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00014, ER_TARGET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CALR3_HUMAN | |
Accessioni | Q96L12Primary (citable) accession number: Q96L12 Secondary accession number(s): D9N574, Q96LN3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 22, 2003 |
Last sequence update: | October 17, 2006 | |
Last modified: | February 10, 2021 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families