UniProtKB - Q96KR7 (PHAR3_HUMAN)
Protein
Phosphatase and actin regulator 3
Gene
PHACTR3
Organism
Homo sapiens (Human)
Status
Functioni
GO - Molecular functioni
- actin binding Source: GO_Central
- protein phosphatase 1 binding Source: InterPro
- protein phosphatase inhibitor activity Source: UniProtKB-KW
GO - Biological processi
- actin cytoskeleton organization Source: GO_Central
Keywordsi
Molecular function | Actin-binding, Protein phosphatase inhibitor |
Enzyme and pathway databases
PathwayCommonsi | Q96KR7 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatase and actin regulator 3Alternative name(s): Scaffold-associated PP1-inhibiting protein Short name: Scapinin |
Gene namesi | Name:PHACTR3 Synonyms:C20orf101, SCAPIN1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000087495.16 |
HGNCi | HGNC:15833, PHACTR3 |
MIMi | 608725, gene |
neXtProti | NX_Q96KR7 |
Subcellular locationi
Nucleus
Note: Localized to the nuclear matrix-intermediate filament scaffold. Isoform 2 is also found in some cytoplasmic extensions.
Nucleus
- nuclear matrix Source: UniProtKB-SubCell
- nucleoplasm Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 116154 |
OpenTargetsi | ENSG00000087495 |
PharmGKBi | PA128394750 |
Miscellaneous databases
Pharosi | Q96KR7, Tbio |
Polymorphism and mutation databases
BioMutai | PHACTR3 |
DMDMi | 38605426 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000126638 | 1 – 559 | Phosphatase and actin regulator 3Add BLAST | 559 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 70 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 230 | PhosphoserineBy similarity | 1 | |
Modified residuei | 236 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q96KR7 |
MassIVEi | Q96KR7 |
PaxDbi | Q96KR7 |
PeptideAtlasi | Q96KR7 |
PRIDEi | Q96KR7 |
ProteomicsDBi | 3118 77109 [Q96KR7-1] 77110 [Q96KR7-2] 77111 [Q96KR7-3] |
PTM databases
iPTMneti | Q96KR7 |
PhosphoSitePlusi | Q96KR7 |
Expressioni
Tissue specificityi
Abundantly expressed in brain. Also found in several tumors such as lung carcinomas, nervous tumors and HL-60 leukemia cells. Isoform 3 is the major form in U-937, GOTO and HL-60 leukemia cells.
Inductioni
Down-regulated in HL-60 leukemia cells by RA, PMA and dimethyl sulfoxide.
Gene expression databases
Bgeei | ENSG00000087495, Expressed in frontal cortex and 154 other tissues |
ExpressionAtlasi | Q96KR7, baseline and differential |
Genevisiblei | Q96KR7, HS |
Organism-specific databases
HPAi | ENSG00000087495, Tissue enriched (brain) |
Interactioni
Subunit structurei
Binds actin and PPP1CA; thus inhibiting the protein phosphatase 1 (PP1) activity.
Binary interactionsi
Hide detailsQ96KR7
GO - Molecular functioni
- actin binding Source: GO_Central
- protein phosphatase 1 binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 125483, 34 interactors |
IntActi | Q96KR7, 34 interactors |
MINTi | Q96KR7 |
STRINGi | 9606.ENSP00000360054 |
Miscellaneous databases
RNActi | Q96KR7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 93 – 118 | RPEL 1Add BLAST | 26 | |
Repeati | 401 – 426 | RPEL 2Add BLAST | 26 | |
Repeati | 439 – 464 | RPEL 3Add BLAST | 26 | |
Repeati | 477 – 502 | RPEL 4Add BLAST | 26 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 438 – 518 | Required for PP1CA binding and inhibition of PP1 activityAdd BLAST | 81 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 346 – 369 | Sequence analysisAdd BLAST | 24 | |
Coiled coili | 450 – 486 | Sequence analysisAdd BLAST | 37 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 217 – 239 | Pro-richAdd BLAST | 23 |
Sequence similaritiesi
Belongs to the phosphatase and actin regulator family.Curated
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG4339, Eukaryota |
GeneTreei | ENSGT00940000157562 |
HOGENOMi | CLU_015753_3_1_1 |
InParanoidi | Q96KR7 |
OMAi | EDCVLQR |
OrthoDBi | 1205245at2759 |
PhylomeDBi | Q96KR7 |
TreeFami | TF316316 |
Family and domain databases
InterProi | View protein in InterPro IPR029990, Phactr3 IPR004018, RPEL_repeat |
PANTHERi | PTHR12751:SF7, PTHR12751:SF7, 1 hit |
Pfami | View protein in Pfam PF02755, RPEL, 1 hit |
SMARTi | View protein in SMART SM00707, RPEL, 4 hits |
PROSITEi | View protein in PROSITE PS51073, RPEL, 4 hits |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96KR7-1) [UniParc]FASTAAdd to basket
Also known as: Scapinin 1B
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAASEDGSGC LVSRGRSQSD PSVLTDSSAT SSADAGENPD EMDQTPPARP
60 70 80 90 100
EYLVSGIRTP PVRRNSKLAT LGRIFKPWKW RKKKNEKLKQ TTSALEKKMA
110 120 130 140 150
GRQGREELIK KGLLEMMEQD AESKTCNPDG GPRSVQSEPP TPKSETLTSE
160 170 180 190 200
DAQPGSPLAT GTDQVSLDKP LSSAAHLDDA AKMPSASSGE EADAGSLLPT
210 220 230 240 250
TNELSQALAG ADSLDSPPRP LERSVGQLPS PPLLPTPPPK ASSKTTKNVT
260 270 280 290 300
GQATLFQASS MKSADPSLRG QLSTPTGSPH LTTVHRPLPP SRVIEELHRA
310 320 330 340 350
LATKHRQDSF QGRESKGSPK KRLDVRLSRT SSVERGKERE EAWSFDGALE
360 370 380 390 400
NKRTAAKESE ENKENLIINS ELKDDLLLYQ DEEALNDSII SGTLPRKCKK
410 420 430 440 450
ELLAVKLRNR PSKQELEDRN IFPRRTDEER QEIRQQIEMK LSKRLSQRPA
460 470 480 490 500
VEELERRNIL KQRNDQTEQE ERREIKQRLT RKLNQRPTVD ELRDRKILIR
510 520 530 540 550
FSDYVEVAKA QDYDRRADKP WTRLSAADKA AIRKELNEYK SNEMEVHASS
KHLTRFHRP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5F9ZHM5 | A0A5F9ZHM5_HUMAN | Phosphatase and actin regulator | PHACTR3 | 128 | Annotation score: | ||
F6RP66 | F6RP66_HUMAN | Phosphatase and actin regulator 3 | PHACTR3 | 41 | Annotation score: |
Sequence cautioni
The sequence CAC67489 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 62 | V → M in AK098788 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 118 | E → G in AK098788 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 321 | K → R in CAF04087 (Ref. 2) Curated | 1 | |
Sequence conflicti | 399 | K → R in CAF04087 (Ref. 2) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021969 | 154 | P → L. Corresponds to variant dbSNP:rs2277759Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009091 | 1 – 41 | Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST | 41 | |
Alternative sequenceiVSP_044546 | 1 – 39 | MAASE…AGENP → MRGRGGGRARCPAPLRSLLG AFGARDAAAAARDPAQ in isoform 4. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_009092 | 181 – 250 | Missing in isoform 3. 1 PublicationAdd BLAST | 70 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000355648; ENSP00000347866; ENSG00000087495 [Q96KR7-2] ENST00000359926; ENSP00000353002; ENSG00000087495 [Q96KR7-4] ENST00000361300; ENSP00000354555; ENSG00000087495 [Q96KR7-3] ENST00000371015; ENSP00000360054; ENSG00000087495 [Q96KR7-1] ENST00000395636; ENSP00000378998; ENSG00000087495 [Q96KR7-2] ENST00000541461; ENSP00000442483; ENSG00000087495 [Q96KR7-2] |
GeneIDi | 116154 |
KEGGi | hsa:116154 |
UCSCi | uc002yat.3, human [Q96KR7-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
SMRi | Q96KR7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 125483, 34 interactors |
IntActi | Q96KR7, 34 interactors |
MINTi | Q96KR7 |
STRINGi | 9606.ENSP00000360054 |
PTM databases
iPTMneti | Q96KR7 |
PhosphoSitePlusi | Q96KR7 |
Polymorphism and mutation databases
BioMutai | PHACTR3 |
DMDMi | 38605426 |
Proteomic databases
EPDi | Q96KR7 |
MassIVEi | Q96KR7 |
PaxDbi | Q96KR7 |
PeptideAtlasi | Q96KR7 |
PRIDEi | Q96KR7 |
ProteomicsDBi | 3118 77109 [Q96KR7-1] 77110 [Q96KR7-2] 77111 [Q96KR7-3] |
Protocols and materials databases
Antibodypediai | 29289, 111 antibodies |
DNASUi | 116154 |
Genome annotation databases
Ensembli | ENST00000355648; ENSP00000347866; ENSG00000087495 [Q96KR7-2] ENST00000359926; ENSP00000353002; ENSG00000087495 [Q96KR7-4] ENST00000361300; ENSP00000354555; ENSG00000087495 [Q96KR7-3] ENST00000371015; ENSP00000360054; ENSG00000087495 [Q96KR7-1] ENST00000395636; ENSP00000378998; ENSG00000087495 [Q96KR7-2] ENST00000541461; ENSP00000442483; ENSG00000087495 [Q96KR7-2] |
GeneIDi | 116154 |
KEGGi | hsa:116154 |
UCSCi | uc002yat.3, human [Q96KR7-1] |
Organism-specific databases
CTDi | 116154 |
DisGeNETi | 116154 |
EuPathDBi | HostDB:ENSG00000087495.16 |
GeneCardsi | PHACTR3 |
HGNCi | HGNC:15833, PHACTR3 |
HPAi | ENSG00000087495, Tissue enriched (brain) |
MIMi | 608725, gene |
neXtProti | NX_Q96KR7 |
OpenTargetsi | ENSG00000087495 |
PharmGKBi | PA128394750 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4339, Eukaryota |
GeneTreei | ENSGT00940000157562 |
HOGENOMi | CLU_015753_3_1_1 |
InParanoidi | Q96KR7 |
OMAi | EDCVLQR |
OrthoDBi | 1205245at2759 |
PhylomeDBi | Q96KR7 |
TreeFami | TF316316 |
Enzyme and pathway databases
PathwayCommonsi | Q96KR7 |
Miscellaneous databases
BioGRID-ORCSi | 116154, 3 hits in 846 CRISPR screens |
ChiTaRSi | PHACTR3, human |
GeneWikii | PHACTR3 |
GenomeRNAii | 116154 |
Pharosi | Q96KR7, Tbio |
PROi | PR:Q96KR7 |
RNActi | Q96KR7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000087495, Expressed in frontal cortex and 154 other tissues |
ExpressionAtlasi | Q96KR7, baseline and differential |
Genevisiblei | Q96KR7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR029990, Phactr3 IPR004018, RPEL_repeat |
PANTHERi | PTHR12751:SF7, PTHR12751:SF7, 1 hit |
Pfami | View protein in Pfam PF02755, RPEL, 1 hit |
SMARTi | View protein in SMART SM00707, RPEL, 4 hits |
PROSITEi | View protein in PROSITE PS51073, RPEL, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHAR3_HUMAN | |
Accessioni | Q96KR7Primary (citable) accession number: Q96KR7 Secondary accession number(s): B1AKX0 Q9H4T4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations