UniProtKB - Q96KQ4 (ASPP1_HUMAN)
Protein
Apoptosis-stimulating of p53 protein 1
Gene
PPP1R13B
Organism
Homo sapiens (Human)
Status
Functioni
Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53 (PubMed:11684014, PubMed:12524540). Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.2 Publications
Miscellaneous
In contrast to its official gene name, it is not a regulatory subunit of protein phosphatase 1. This name was given due to its similarity with a protein that binds to protein phosphatase 1.
GO - Molecular functioni
- p53 binding Source: GO_Central
- transcription factor binding Source: InterPro
GO - Biological processi
- intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
- negative regulation of cell cycle Source: UniProtKB
- positive regulation of neuron death Source: Ensembl
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
- regulation of apoptotic process Source: Reactome
- regulation of signal transduction by p53 class mediator Source: Reactome
Keywordsi
Biological process | Apoptosis |
Enzyme and pathway databases
PathwayCommonsi | Q96KQ4 |
Reactomei | R-HSA-139915, Activation of PUMA and translocation to mitochondria R-HSA-6803204, TP53 Regulates Transcription of Genes Involved in Cytochrome C Release R-HSA-6803205, TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain R-HSA-6803211, TP53 Regulates Transcription of Death Receptors and Ligands R-HSA-6804759, Regulation of TP53 Activity through Association with Co-factors |
Names & Taxonomyi
Protein namesi | Recommended name: Apoptosis-stimulating of p53 protein 1Alternative name(s): Protein phosphatase 1 regulatory subunit 13B |
Gene namesi | Name:PPP1R13B Synonyms:ASPP1, KIAA0771 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14950, PPP1R13B |
MIMi | 606455, gene |
neXtProti | NX_Q96KQ4 |
VEuPathDBi | HostDB:ENSG00000088808.16 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Mitochondrion
- mitochondrion Source: GOC
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: UniProtKB
- perinuclear region of cytoplasm Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 23368 |
OpenTargetsi | ENSG00000088808 |
PharmGKBi | PA33622 |
Miscellaneous databases
Pharosi | Q96KQ4, Tbio |
Genetic variation databases
BioMutai | PPP1R13B |
DMDMi | 296439434 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000066962 | 1 – 1090 | Apoptosis-stimulating of p53 protein 1Add BLAST | 1090 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 332 | PhosphoserineCombined sources | 1 | |
Modified residuei | 335 | PhosphoserineCombined sources | 1 | |
Modified residuei | 554 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 681 | PhosphoserineCombined sources | 1 | |
Modified residuei | 710 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q96KQ4 |
jPOSTi | Q96KQ4 |
MassIVEi | Q96KQ4 |
MaxQBi | Q96KQ4 |
PaxDbi | Q96KQ4 |
PeptideAtlasi | Q96KQ4 |
PRIDEi | Q96KQ4 |
ProteomicsDBi | 77101 |
PTM databases
CarbonylDBi | Q96KQ4 |
iPTMneti | Q96KQ4 |
PhosphoSitePlusi | Q96KQ4 |
Expressioni
Tissue specificityi
Reduced expression in breast carcinomas expressing a wild-type TP53 protein.1 Publication
Gene expression databases
Bgeei | ENSG00000088808, Expressed in testis and 229 other tissues |
ExpressionAtlasi | Q96KQ4, baseline and differential |
Genevisiblei | Q96KQ4, HS |
Organism-specific databases
HPAi | ENSG00000088808, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with P53/TP53; the interaction promotes pro-apoptotic activity.
2 PublicationsBinary interactionsi
Q96KQ4
GO - Molecular functioni
- p53 binding Source: GO_Central
- transcription factor binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 116948, 123 interactors |
CORUMi | Q96KQ4 |
ELMi | Q96KQ4 |
IntActi | Q96KQ4, 94 interactors |
MINTi | Q96KQ4 |
STRINGi | 9606.ENSP00000202556 |
Miscellaneous databases
RNActi | Q96KQ4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 920 – 952 | ANK 1Add BLAST | 33 | |
Repeati | 953 – 985 | ANK 2Add BLAST | 33 | |
Domaini | 1019 – 1081 | SH3PROSITE-ProRule annotationAdd BLAST | 63 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 132 – 292 | Gln-richAdd BLAST | 161 | |
Compositional biasi | 428 – 860 | Pro-richAdd BLAST | 433 |
Domaini
The ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
Sequence similaritiesi
Belongs to the ASPP family.Curated
Keywords - Domaini
ANK repeat, Repeat, SH3 domainPhylogenomic databases
eggNOGi | KOG0515, Eukaryota |
GeneTreei | ENSGT00940000153463 |
HOGENOMi | CLU_008234_0_0_1 |
InParanoidi | Q96KQ4 |
OMAi | EGLICPP |
OrthoDBi | 1041229at2759 |
PhylomeDBi | Q96KQ4 |
TreeFami | TF105545 |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR028319, ASPP_1 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR24131:SF5, PTHR24131:SF5, 1 hit |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00248, ANK, 2 hits SM00326, SH3, 1 hit |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF50044, SSF50044, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 2 hits PS50002, SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
Q96KQ4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMPMILTVFL SNNEQILTEV PITPETTCRD VVEFCKEPGE GSCHLAEVWR
60 70 80 90 100
GNERPIPFDH MMYEHLQKWG PRREEVKFFL RHEDSPTENS EQGGRQTQEQ
110 120 130 140 150
RTQRNVINVP GEKRTENGVG NPRVELTLSE LQDMAARQQQ QIENQQQMLV
160 170 180 190 200
AKEQRLHFLK QQERRQQQSI SENEKLQKLK ERVEAQENKL KKIRAMRGQV
210 220 230 240 250
DYSKIMNGNL SAEIERFSAM FQEKKQEVQT AILRVDQLSQ QLEDLKKGKL
260 270 280 290 300
NGFQSYNGKL TGPAAVELKR LYQELQIRNQ LNQEQNSKLQ QQKELLNKRN
310 320 330 340 350
MEVAMMDKRI SELRERLYGK KIQLNRVNGT SSPQSPLSTS GRVAAVGPYI
360 370 380 390 400
QVPSAGSFPV LGDPIKPQSL SIASNAAHGR SKSANDGNWP TLKQNSSSSV
410 420 430 440 450
KPVQVAGADW KDPSVEGSVK QGTVSSQPVP FSALGPTEKP GIEIGKVPPP
460 470 480 490 500
IPGVGKQLPP SYGTYPSPTP LGPGSTSSLE RRKEGSLPRP SAGLPSRQRP
510 520 530 540 550
TLLPATGSTP QPGSSQQIQQ RISVPPSPTY PPAGPPAFPA GDSKPELPLT
560 570 580 590 600
VAIRPFLADK GSRPQSPRKG PQTVNSSSIY SMYLQQATPP KNYQPAAHSA
610 620 630 640 650
LNKSVKAVYG KPVLPSGSTS PSPLPFLHGS LSTGTPQPQP PSESTEKEPE
660 670 680 690 700
QDGPAAPADG STVESLPRPL SPTKLTPIVH SPLRYQSDAD LEALRRKLAN
710 720 730 740 750
APRPLKKRSS ITEPEGPGGP NIQKLLYQRF NTLAGGMEGT PFYQPSPSQD
760 770 780 790 800
FMGTLADVDN GNTNANGNLE ELPPAQPTAP LPAEPAPSSD ANDNELPSPE
810 820 830 840 850
PEELICPQTT HQTAEPAEDN NNNVATVPTT EQIPSPVAEA PSPGEEQVPP
860 870 880 890 900
APLPPASHPP ATSTNKRTNL KKPNSERTGH GLRVRFNPLA LLLDASLEGE
910 920 930 940 950
FDLVQRIIYE VEDPSKPNDE GITPLHNAVC AGHHHIVKFL LDFGVNVNAA
960 970 980 990 1000
DSDGWTPLHC AASCNSVHLC KQLVESGAAI FASTISDIET AADKCEEMEE
1010 1020 1030 1040 1050
GYIQCSQFLY GVQEKLGVMN KGVAYALWDY EAQNSDELSF HEGDALTILR
1060 1070 1080 1090
RKDESETEWW WARLGDREGY VPKNLLGLYP RIKPRQRTLA
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3B3ISJ2 | A0A3B3ISJ2_HUMAN | Apoptosis-stimulating of p53 protei... | PPP1R13B | 1,075 | Annotation score: | ||
H0YJL1 | H0YJL1_HUMAN | Apoptosis-stimulating of p53 protei... | PPP1R13B | 635 | Annotation score: | ||
G3V5J1 | G3V5J1_HUMAN | Apoptosis-stimulating of p53 protei... | PPP1R13B | 149 | Annotation score: | ||
G3V2L3 | G3V2L3_HUMAN | Apoptosis-stimulating of p53 protei... | PPP1R13B | 143 | Annotation score: | ||
G3V3S2 | G3V3S2_HUMAN | Apoptosis-stimulating of p53 protei... | PPP1R13B | 122 | Annotation score: | ||
H0YJG1 | H0YJG1_HUMAN | Apoptosis-stimulating of p53 protei... | PPP1R13B | 106 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 68 | K → I in CAC83011 (PubMed:11684014).Curated | 1 | |
Sequence conflicti | 112 | E → D in CAC83011 (PubMed:11684014).Curated | 1 | |
Sequence conflicti | 117 | N → Y in CAC83011 (PubMed:11684014).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ318887 mRNA Translation: CAC83011.2 AL049840 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW81847.1 BC136527 mRNA Translation: AAI36528.1 AB018314 mRNA Translation: BAA34491.1 |
CCDSi | CCDS41997.1 |
RefSeqi | NP_056131.2, NM_015316.2 |
Genome annotation databases
Ensembli | ENST00000202556; ENSP00000202556; ENSG00000088808 |
GeneIDi | 23368 |
KEGGi | hsa:23368 |
UCSCi | uc001yof.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ318887 mRNA Translation: CAC83011.2 AL049840 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW81847.1 BC136527 mRNA Translation: AAI36528.1 AB018314 mRNA Translation: BAA34491.1 |
CCDSi | CCDS41997.1 |
RefSeqi | NP_056131.2, NM_015316.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6HL5 | X-ray | 1.98 | S | 932-954 | [»] | |
SMRi | Q96KQ4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116948, 123 interactors |
CORUMi | Q96KQ4 |
ELMi | Q96KQ4 |
IntActi | Q96KQ4, 94 interactors |
MINTi | Q96KQ4 |
STRINGi | 9606.ENSP00000202556 |
PTM databases
CarbonylDBi | Q96KQ4 |
iPTMneti | Q96KQ4 |
PhosphoSitePlusi | Q96KQ4 |
Genetic variation databases
BioMutai | PPP1R13B |
DMDMi | 296439434 |
Proteomic databases
EPDi | Q96KQ4 |
jPOSTi | Q96KQ4 |
MassIVEi | Q96KQ4 |
MaxQBi | Q96KQ4 |
PaxDbi | Q96KQ4 |
PeptideAtlasi | Q96KQ4 |
PRIDEi | Q96KQ4 |
ProteomicsDBi | 77101 |
Protocols and materials databases
Antibodypediai | 87, 224 antibodies |
Genome annotation databases
Ensembli | ENST00000202556; ENSP00000202556; ENSG00000088808 |
GeneIDi | 23368 |
KEGGi | hsa:23368 |
UCSCi | uc001yof.2, human |
Organism-specific databases
CTDi | 23368 |
DisGeNETi | 23368 |
GeneCardsi | PPP1R13B |
HGNCi | HGNC:14950, PPP1R13B |
HPAi | ENSG00000088808, Low tissue specificity |
MIMi | 606455, gene |
neXtProti | NX_Q96KQ4 |
OpenTargetsi | ENSG00000088808 |
PharmGKBi | PA33622 |
VEuPathDBi | HostDB:ENSG00000088808.16 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0515, Eukaryota |
GeneTreei | ENSGT00940000153463 |
HOGENOMi | CLU_008234_0_0_1 |
InParanoidi | Q96KQ4 |
OMAi | EGLICPP |
OrthoDBi | 1041229at2759 |
PhylomeDBi | Q96KQ4 |
TreeFami | TF105545 |
Enzyme and pathway databases
PathwayCommonsi | Q96KQ4 |
Reactomei | R-HSA-139915, Activation of PUMA and translocation to mitochondria R-HSA-6803204, TP53 Regulates Transcription of Genes Involved in Cytochrome C Release R-HSA-6803205, TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain R-HSA-6803211, TP53 Regulates Transcription of Death Receptors and Ligands R-HSA-6804759, Regulation of TP53 Activity through Association with Co-factors |
Miscellaneous databases
BioGRID-ORCSi | 23368, 11 hits in 995 CRISPR screens |
ChiTaRSi | PPP1R13B, human |
GeneWikii | PPP1R13B |
GenomeRNAii | 23368 |
Pharosi | Q96KQ4, Tbio |
PROi | PR:Q96KQ4 |
RNActi | Q96KQ4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000088808, Expressed in testis and 229 other tissues |
ExpressionAtlasi | Q96KQ4, baseline and differential |
Genevisiblei | Q96KQ4, HS |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR028319, ASPP_1 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR24131:SF5, PTHR24131:SF5, 1 hit |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00248, ANK, 2 hits SM00326, SH3, 1 hit |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF50044, SSF50044, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 2 hits PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASPP1_HUMAN | |
Accessioni | Q96KQ4Primary (citable) accession number: Q96KQ4 Secondary accession number(s): B2RMX5, O94870 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 15, 2003 |
Last sequence update: | May 18, 2010 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families