UniProtKB - Q96KN8 (PLAT5_HUMAN)
Protein
Phospholipase A and acyltransferase 5
Gene
PLAAT5
Organism
Homo sapiens (Human)
Status
Functioni
Exhibits both phospholipase A1/2 and acyltransferase activities (PubMed:22825852, PubMed:26503625). Shows phospholipase A1 (PLA1) and A2 (PLA2) activity, catalyzing the calcium-independent release of fatty acids from the sn-1 or sn-2 position of glycerophospholipids (PubMed:22825852). Shows N-acyltransferase activity, catalyzing the calcium-independent transfer of a fatty acyl group at the sn-1 position of phosphatidylcholine (PC) and other glycerophospholipids to the primary amine of phosphatidylethanolamine (PE), forming N-acylphosphatidylethanolamine (NAPE), which serves as precursor for N-acylethanolamines (NAEs) (PubMed:19000777, PubMed:22825852).1 Publication2 Publications
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+2 PublicationsEC:3.1.1.42 Publications
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+2 PublicationsEC:3.1.1.322 Publications
- 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine = 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+ + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+ + N-hexadecanoyl-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine = 2-hexadecanoyl-sn-glycero-3-phosphocholine + H+ + N-hexadecanoyl-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- a 1,2-diacyl-sn-glycero-3-phosphocholine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = a 1-acyl-sn-glycero-3-phosphocholine + H+ + N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- a 1,2-diacyl-sn-glycero-3-phosphocholine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = a 2-acyl-sn-glycero-3-phosphocholine + H+ + N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine = 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+ + N,1,2-tri-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamineBy similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 145 | PROSITE-ProRule annotation | 1 | |
Active sitei | 157 | PROSITE-ProRule annotation | 1 | |
Active sitei | 233 | Acyl-thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 1-acyl-2-lysophosphatidylserine acylhydrolase activity Source: UniProtKB-EC
- N-acyltransferase activity Source: UniProtKB
- phosphatidylserine 1-acylhydrolase activity Source: UniProtKB-EC
- phospholipase A1 activity Source: UniProtKB
- phospholipase A2 activity Source: UniProtKB
- phospholipase A2 activity (consuming 1,2-dipalmitoylphosphatidylcholine) Source: UniProtKB-EC
- phospholipase A2 activity consuming 1,2-dioleoylphosphatidylethanolamine) Source: UniProtKB-EC
GO - Biological processi
- N-acylphosphatidylethanolamine metabolic process Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Hydrolase, Transferase |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000168004-MONOMER |
PathwayCommonsi | Q96KN8 |
Reactomei | R-HSA-1482839, Acyl chain remodelling of PE |
Chemistry databases
SwissLipidsi | SLP:000001126 |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipase A and acyltransferase 5ImportedAlternative name(s): |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24978, PLAAT5 |
MIMi | 611474, gene |
neXtProti | NX_Q96KN8 |
VEuPathDBi | HostDB:ENSG00000168004.9 |
Pathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000168004 |
PharmGKBi | PA142671672 |
Miscellaneous databases
Pharosi | Q96KN8, Tbio |
Genetic variation databases
BioMutai | HRASLS5 |
DMDMi | 296434533 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000152488 | 1 – 279 | Phospholipase A and acyltransferase 5Add BLAST | 279 |
Proteomic databases
MassIVEi | Q96KN8 |
PaxDbi | Q96KN8 |
PeptideAtlasi | Q96KN8 |
PRIDEi | Q96KN8 |
ProteomicsDBi | 24961 26717 77095 [Q96KN8-1] |
PTM databases
iPTMneti | Q96KN8 |
Expressioni
Tissue specificityi
Highest expression level in testis and pancreas.
Gene expression databases
Bgeei | ENSG00000168004, Expressed in testis and 150 other tissues |
ExpressionAtlasi | Q96KN8, baseline and differential |
Genevisiblei | Q96KN8, HS |
Organism-specific databases
HPAi | ENSG00000168004, Tissue enhanced (blood, testis) |
Interactioni
Binary interactionsi
Hide detailsIsoform 3 [Q96KN8-3]
With | #Exp. | IntAct |
---|---|---|
GGACT [Q9BVM4] | 8 | EBI-10290304,EBI-10299852 |
GOLGA2 [Q08379] | 6 | EBI-10290304,EBI-618309 |
HOMER3 [Q9NSC5] | 3 | EBI-10290304,EBI-748420 |
ZNF783 [Q6ZMS7] | 3 | EBI-10290304,EBI-10254978 |
Protein-protein interaction databases
BioGRIDi | 125580, 7 interactors |
IntActi | Q96KN8, 5 interactors |
STRINGi | 9606.ENSP00000301790 |
Miscellaneous databases
RNActi | Q96KN8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 135 – 249 | LRATPROSITE-ProRule annotationAdd BLAST | 115 |
Sequence similaritiesi
Belongs to the H-rev107 family.Curated
Phylogenomic databases
eggNOGi | ENOG502S0JN, Eukaryota |
GeneTreei | ENSGT00940000162436 |
HOGENOMi | CLU_070482_0_0_1 |
InParanoidi | Q96KN8 |
OMAi | TGPKDQP |
OrthoDBi | 1602481at2759 |
PhylomeDBi | Q96KN8 |
TreeFami | TF330836 |
Family and domain databases
InterProi | View protein in InterPro IPR007053, LRAT_dom |
Pfami | View protein in Pfam PF04970, LRAT, 1 hit |
PROSITEi | View protein in PROSITE PS51934, LRAT, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96KN8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGLSPGAEGE YALRLPRIPP PLPKPASRTA STGPKDQPPA LRRSAVPHSG
60 70 80 90 100
LNSISPLELE ESVGFAALVQ LPAKQPPPGT LEQGRSIQQG EKAVVSLETT
110 120 130 140 150
PSQKADWSSI PKPENEGKLI KQAAEGKPRP RPGDLIEIFR IGYEHWAIYV
160 170 180 190 200
EDDCVVHLAP PSEEFEVGSI TSIFSNRAVV KYSRLEDVLH GCSWKVNNKL
210 220 230 240 250
DGTYLPLPVD KIIQRTKKMV NKIVQYSLIE GNCEHFVNGL RYGVPRSQQV
260 270
EHALMEGAKA AGAVISAVVD SIKPKPITA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YGT7 | H0YGT7_HUMAN | Phospholipase A and acyltransferase... | PLAAT5 | 49 | Annotation score: | ||
H7BYL7 | H7BYL7_HUMAN | Phospholipase A and acyltransferase... | PLAAT5 | 37 | Annotation score: | ||
F5H4Q3 | F5H4Q3_HUMAN | Phospholipase A and acyltransferase... | PLAAT5 | 19 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049477 | 31 | S → G3 PublicationsCorresponds to variant dbSNP:rs10897424Ensembl. | 1 | |
Natural variantiVAR_024486 | 93 | A → P. Corresponds to variant dbSNP:rs940611Ensembl. | 1 | |
Natural variantiVAR_049478 | 214 | Q → R. Corresponds to variant dbSNP:rs35735923Ensembl. | 1 | |
Natural variantiVAR_049479 | 258 | A → V. Corresponds to variant dbSNP:rs35375575Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045825 | 50 – 59 | Missing in isoform 3. Curated | 10 | |
Alternative sequenceiVSP_045826 | 234 – 279 | EHFVN…KPITA → RARPDGRSEGCWSSYFSCSG in isoform 2. CuratedAdd BLAST | 46 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB298804 mRNA Translation: BAH08633.1 AJ416558 mRNA Translation: CAC94942.1 AP000484 Genomic DNA No translation available. AP001591 Genomic DNA No translation available. CH471076 Genomic DNA Translation: EAW74150.1 |
CCDSi | CCDS53646.1 [Q96KN8-3] CCDS53647.1 [Q96KN8-2] CCDS8044.1 [Q96KN8-1] |
RefSeqi | NP_473449.1, NM_054108.3 [Q96KN8-1] |
Genome annotation databases
Ensembli | ENST00000301790; ENSP00000301790; ENSG00000168004 [Q96KN8-1] ENST00000539221; ENSP00000443873; ENSG00000168004 [Q96KN8-2] ENST00000540857; ENSP00000444809; ENSG00000168004 [Q96KN8-3] |
GeneIDi | 117245 |
KEGGi | hsa:117245 |
UCSCi | uc001nwy.3, human [Q96KN8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB298804 mRNA Translation: BAH08633.1 AJ416558 mRNA Translation: CAC94942.1 AP000484 Genomic DNA No translation available. AP001591 Genomic DNA No translation available. CH471076 Genomic DNA Translation: EAW74150.1 |
CCDSi | CCDS53646.1 [Q96KN8-3] CCDS53647.1 [Q96KN8-2] CCDS8044.1 [Q96KN8-1] |
RefSeqi | NP_473449.1, NM_054108.3 [Q96KN8-1] |
3D structure databases
SMRi | Q96KN8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 125580, 7 interactors |
IntActi | Q96KN8, 5 interactors |
STRINGi | 9606.ENSP00000301790 |
Chemistry databases
SwissLipidsi | SLP:000001126 |
PTM databases
iPTMneti | Q96KN8 |
Genetic variation databases
BioMutai | HRASLS5 |
DMDMi | 296434533 |
Proteomic databases
MassIVEi | Q96KN8 |
PaxDbi | Q96KN8 |
PeptideAtlasi | Q96KN8 |
PRIDEi | Q96KN8 |
ProteomicsDBi | 24961 26717 77095 [Q96KN8-1] |
Protocols and materials databases
Antibodypediai | 43875, 81 antibodies |
DNASUi | 117245 |
Genome annotation databases
Ensembli | ENST00000301790; ENSP00000301790; ENSG00000168004 [Q96KN8-1] ENST00000539221; ENSP00000443873; ENSG00000168004 [Q96KN8-2] ENST00000540857; ENSP00000444809; ENSG00000168004 [Q96KN8-3] |
GeneIDi | 117245 |
KEGGi | hsa:117245 |
UCSCi | uc001nwy.3, human [Q96KN8-1] |
Organism-specific databases
CTDi | 117245 |
GeneCardsi | PLAAT5 |
HGNCi | HGNC:24978, PLAAT5 |
HPAi | ENSG00000168004, Tissue enhanced (blood, testis) |
MIMi | 611474, gene |
neXtProti | NX_Q96KN8 |
OpenTargetsi | ENSG00000168004 |
PharmGKBi | PA142671672 |
VEuPathDBi | HostDB:ENSG00000168004.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S0JN, Eukaryota |
GeneTreei | ENSGT00940000162436 |
HOGENOMi | CLU_070482_0_0_1 |
InParanoidi | Q96KN8 |
OMAi | TGPKDQP |
OrthoDBi | 1602481at2759 |
PhylomeDBi | Q96KN8 |
TreeFami | TF330836 |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000168004-MONOMER |
PathwayCommonsi | Q96KN8 |
Reactomei | R-HSA-1482839, Acyl chain remodelling of PE |
Miscellaneous databases
BioGRID-ORCSi | 117245, 9 hits in 874 CRISPR screens |
ChiTaRSi | HRASLS5, human |
GenomeRNAii | 117245 |
Pharosi | Q96KN8, Tbio |
PROi | PR:Q96KN8 |
RNActi | Q96KN8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000168004, Expressed in testis and 150 other tissues |
ExpressionAtlasi | Q96KN8, baseline and differential |
Genevisiblei | Q96KN8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR007053, LRAT_dom |
Pfami | View protein in Pfam PF04970, LRAT, 1 hit |
PROSITEi | View protein in PROSITE PS51934, LRAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLAT5_HUMAN | |
Accessioni | Q96KN8Primary (citable) accession number: Q96KN8 Secondary accession number(s): B7X6T1, F5GZ87, F5H4Y9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 31, 2002 |
Last sequence update: | May 18, 2010 | |
Last modified: | February 10, 2021 | |
This is version 128 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families