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Protein

Lymphokine-activated killer T-cell-originated protein kinase

Gene

PBK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Activity regulationi

Activated by phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64ATPPROSITE-ProRule annotation1
Active sitei167Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 46ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ96KB5
SIGNORiQ96KB5

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphokine-activated killer T-cell-originated protein kinase (EC:2.7.12.2)
Alternative name(s):
Cancer/testis antigen 84
Short name:
CT84
MAPKK-like protein kinase
Nori-3
PDZ-binding kinase
Spermatogenesis-related protein kinase
Short name:
SPK
T-LAK cell-originated protein kinase
Gene namesi
Name:PBK
Synonyms:TOPK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000168078.9
HGNCiHGNC:18282 PBK
MIMi611210 gene
neXtProtiNX_Q96KB5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9T → E: TP53-binding. 1 Publication1
Mutagenesisi64 – 65KK → AA: Loss of activity. 1 Publication2
Mutagenesisi320T → A: Decrease in the binding to DLG1. 1 Publication1
Mutagenesisi322V → A: Decrease in the binding to DLG1. 1 Publication1

Organism-specific databases

DisGeNETi55872
OpenTargetsiENSG00000168078
PharmGKBiPA134925802

Chemistry databases

ChEMBLiCHEMBL4896
GuidetoPHARMACOLOGYi2140

Polymorphism and mutation databases

BioMutaiPBK
DMDMi83305809

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867631 – 322Lymphokine-activated killer T-cell-originated protein kinaseAdd BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei9PhosphothreonineCombined sources1
Modified residuei24PhosphothreonineCombined sources1
Modified residuei32PhosphoserineCombined sources1
Modified residuei59PhosphoserineCombined sources1
Cross-linki169Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Phosphorylated; in a cell-cycle dependent manner at mitosis.2 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96KB5
PaxDbiQ96KB5
PeptideAtlasiQ96KB5
PRIDEiQ96KB5
ProteomicsDBi77056

PTM databases

iPTMnetiQ96KB5
PhosphoSitePlusiQ96KB5

Expressioni

Tissue specificityi

Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.2 Publications

Gene expression databases

BgeeiENSG00000168078 Expressed in 128 organ(s), highest expression level in sperm
CleanExiHS_PBK
ExpressionAtlasiQ96KB5 baseline and differential
GenevisibleiQ96KB5 HS

Organism-specific databases

HPAiHPA005753
HPA050656

Interactioni

Subunit structurei

Interacts with DLG1 and TP53.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi120971, 31 interactors
IntActiQ96KB5, 12 interactors
MINTiQ96KB5
STRINGi9606.ENSP00000301905

Chemistry databases

BindingDBiQ96KB5

Structurei

Secondary structure

1322
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96KB5
SMRiQ96KB5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 322Protein kinasePROSITE-ProRule annotationAdd BLAST291

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni320 – 322PDZ-interaction3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0192 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00720000108839
HOGENOMiHOG000294208
HOVERGENiHBG056011
InParanoidiQ96KB5
KOiK08865
OMAiSMCLAME
OrthoDBiEOG091G05V7
PhylomeDBiQ96KB5
TreeFamiTF329763

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
IPR034368 TOPK
PANTHERiPTHR43289:SF3 PTHR43289:SF3, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96KB5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEGISNFKTP SKLSEKKKSV LCSTPTINIP ASPFMQKLGF GTGVNVYLMK
60 70 80 90 100
RSPRGLSHSP WAVKKINPIC NDHYRSVYQK RLMDEAKILK SLHHPNIVGY
110 120 130 140 150
RAFTEANDGS LCLAMEYGGE KSLNDLIEER YKASQDPFPA AIILKVALNM
160 170 180 190 200
ARGLKYLHQE KKLLHGDIKS SNVVIKGDFE TIKICDVGVS LPLDENMTVT
210 220 230 240 250
DPEACYIGTE PWKPKEAVEE NGVITDKADI FAFGLTLWEM MTLSIPHINL
260 270 280 290 300
SNDDDDEDKT FDESDFDDEA YYAALGTRPP INMEELDESY QKVIELFSVC
310 320
TNEDPKDRPS AAHIVEALET DV
Length:322
Mass (Da):36,085
Last modified:December 6, 2005 - v3
Checksum:i6BF55789BC204ABE
GO
Isoform 2 (identifier: Q96KB5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-198: T → TAPAFITILLVS

Note: No experimental confirmation available.
Show »
Length:333
Mass (Da):37,211
Checksum:i05A472E8DD4D9928
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RFX4E5RFX4_HUMAN
Lymphokine-activated killer T-cell-...
PBK
151Annotation score:
E5RIE1E5RIE1_HUMAN
Lymphokine-activated killer T-cell-...
PBK
63Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34F → I in AAF71521 (PubMed:11378444).Curated1
Sequence conflicti169K → E in BAB55019 (PubMed:14702039).Curated1
Sequence conflicti254D → N in BAB55019 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021162107N → S4 PublicationsCorresponds to variant dbSNP:rs3779620Ensembl.1
Natural variantiVAR_051676220E → D. Corresponds to variant dbSNP:rs17057901Ensembl.1
Natural variantiVAR_041234241M → L1 PublicationCorresponds to variant dbSNP:rs36086402Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055269198T → TAPAFITILLVS in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027249 mRNA Translation: BAA99576.1
AB027250 mRNA Translation: BAA99577.1
AF189722 mRNA Translation: AAF69107.1
AF237709 mRNA Translation: AAF71521.1
AK027291 mRNA Translation: BAB55019.1
AK301836 mRNA Translation: BAG63280.1
AC104997 Genomic DNA No translation available.
CH471080 Genomic DNA Translation: EAW63536.1
CH471080 Genomic DNA Translation: EAW63537.1
BC015191 mRNA Translation: AAH15191.1
CCDSiCCDS6063.1 [Q96KB5-1]
CCDS64858.1 [Q96KB5-2]
RefSeqiNP_001265874.1, NM_001278945.1 [Q96KB5-2]
NP_060962.2, NM_018492.3 [Q96KB5-1]
XP_006716431.1, XM_006716368.2
UniGeneiHs.104741

Genome annotation databases

EnsembliENST00000301905; ENSP00000301905; ENSG00000168078 [Q96KB5-1]
ENST00000522944; ENSP00000428489; ENSG00000168078 [Q96KB5-2]
GeneIDi55872
KEGGihsa:55872
UCSCiuc011lap.4 human [Q96KB5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027249 mRNA Translation: BAA99576.1
AB027250 mRNA Translation: BAA99577.1
AF189722 mRNA Translation: AAF69107.1
AF237709 mRNA Translation: AAF71521.1
AK027291 mRNA Translation: BAB55019.1
AK301836 mRNA Translation: BAG63280.1
AC104997 Genomic DNA No translation available.
CH471080 Genomic DNA Translation: EAW63536.1
CH471080 Genomic DNA Translation: EAW63537.1
BC015191 mRNA Translation: AAH15191.1
CCDSiCCDS6063.1 [Q96KB5-1]
CCDS64858.1 [Q96KB5-2]
RefSeqiNP_001265874.1, NM_001278945.1 [Q96KB5-2]
NP_060962.2, NM_018492.3 [Q96KB5-1]
XP_006716431.1, XM_006716368.2
UniGeneiHs.104741

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5J0AX-ray2.74A23-320[»]
B19-319[»]
ProteinModelPortaliQ96KB5
SMRiQ96KB5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120971, 31 interactors
IntActiQ96KB5, 12 interactors
MINTiQ96KB5
STRINGi9606.ENSP00000301905

Chemistry databases

BindingDBiQ96KB5
ChEMBLiCHEMBL4896
GuidetoPHARMACOLOGYi2140

PTM databases

iPTMnetiQ96KB5
PhosphoSitePlusiQ96KB5

Polymorphism and mutation databases

BioMutaiPBK
DMDMi83305809

Proteomic databases

EPDiQ96KB5
PaxDbiQ96KB5
PeptideAtlasiQ96KB5
PRIDEiQ96KB5
ProteomicsDBi77056

Protocols and materials databases

DNASUi55872
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000301905; ENSP00000301905; ENSG00000168078 [Q96KB5-1]
ENST00000522944; ENSP00000428489; ENSG00000168078 [Q96KB5-2]
GeneIDi55872
KEGGihsa:55872
UCSCiuc011lap.4 human [Q96KB5-1]

Organism-specific databases

CTDi55872
DisGeNETi55872
EuPathDBiHostDB:ENSG00000168078.9
GeneCardsiPBK
HGNCiHGNC:18282 PBK
HPAiHPA005753
HPA050656
MIMi611210 gene
neXtProtiNX_Q96KB5
OpenTargetsiENSG00000168078
PharmGKBiPA134925802
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0192 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00720000108839
HOGENOMiHOG000294208
HOVERGENiHBG056011
InParanoidiQ96KB5
KOiK08865
OMAiSMCLAME
OrthoDBiEOG091G05V7
PhylomeDBiQ96KB5
TreeFamiTF329763

Enzyme and pathway databases

SignaLinkiQ96KB5
SIGNORiQ96KB5

Miscellaneous databases

ChiTaRSiPBK human
GeneWikiiPBK_(gene)
GenomeRNAii55872
PROiPR:Q96KB5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168078 Expressed in 128 organ(s), highest expression level in sperm
CleanExiHS_PBK
ExpressionAtlasiQ96KB5 baseline and differential
GenevisibleiQ96KB5 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
IPR034368 TOPK
PANTHERiPTHR43289:SF3 PTHR43289:SF3, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTOPK_HUMAN
AccessioniPrimary (citable) accession number: Q96KB5
Secondary accession number(s): B4DX68
, D3DST2, Q9NPD9, Q9NYL7, Q9NZK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 6, 2005
Last modified: November 7, 2018
This is version 157 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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