UniProtKB - Q96KB5 (TOPK_HUMAN)
Protein
Lymphokine-activated killer T-cell-originated protein kinase
Gene
PBK
Organism
Homo sapiens (Human)
Status
Functioni
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.2 Publications
Catalytic activityi
Activity regulationi
Activated by phosphorylation.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 64 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 167 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 38 – 46 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase kinase activity Source: UniProtKB-EC
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: RHEA
- protein threonine kinase activity Source: RHEA
- transmembrane receptor protein tyrosine kinase activity Source: RHEA
GO - Biological processi
- cellular response to UV Source: Ensembl
- mitotic cell cycle Source: UniProtKB
- negative regulation of inflammatory response Source: Ensembl
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: Ensembl
- negative regulation of protein phosphorylation Source: Ensembl
- negative regulation of stress-activated MAPK cascade Source: Ensembl
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q96KB5 |
SignaLinki | Q96KB5 |
SIGNORi | Q96KB5 |
Names & Taxonomyi
Protein namesi | Recommended name: Lymphokine-activated killer T-cell-originated protein kinase (EC:2.7.12.2)Alternative name(s): Cancer/testis antigen 84 Short name: CT84 MAPKK-like protein kinase Nori-3 PDZ-binding kinase Spermatogenesis-related protein kinase Short name: SPK T-LAK cell-originated protein kinase |
Gene namesi | Name:PBK Synonyms:TOPK |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18282, PBK |
MIMi | 611210, gene |
neXtProti | NX_Q96KB5 |
VEuPathDBi | HostDB:ENSG00000168078.9 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | T → E: TP53-binding. 1 Publication | 1 | |
Mutagenesisi | 64 – 65 | KK → AA: Loss of activity. 1 Publication | 2 | |
Mutagenesisi | 320 | T → A: Decrease in the binding to DLG1. 1 Publication | 1 | |
Mutagenesisi | 322 | V → A: Decrease in the binding to DLG1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 55872 |
OpenTargetsi | ENSG00000168078 |
PharmGKBi | PA134925802 |
Miscellaneous databases
Pharosi | Q96KB5, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4896 |
GuidetoPHARMACOLOGYi | 2140 |
Genetic variation databases
BioMutai | PBK |
DMDMi | 83305809 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086763 | 1 – 322 | Lymphokine-activated killer T-cell-originated protein kinaseAdd BLAST | 322 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 9 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 24 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 32 | PhosphoserineCombined sources | 1 | |
Modified residuei | 59 | PhosphoserineCombined sources | 1 | |
Cross-linki | 169 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Post-translational modificationi
Phosphorylated; in a cell-cycle dependent manner at mitosis.2 Publications
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q96KB5 |
jPOSTi | Q96KB5 |
MassIVEi | Q96KB5 |
PaxDbi | Q96KB5 |
PeptideAtlasi | Q96KB5 |
PRIDEi | Q96KB5 |
ProteomicsDBi | 5416 77056 [Q96KB5-1] |
PTM databases
iPTMneti | Q96KB5 |
PhosphoSitePlusi | Q96KB5 |
Expressioni
Tissue specificityi
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.2 Publications
Gene expression databases
Bgeei | ENSG00000168078, Expressed in ventricular zone and 147 other tissues |
ExpressionAtlasi | Q96KB5, baseline and differential |
Genevisiblei | Q96KB5, HS |
Organism-specific databases
HPAi | ENSG00000168078, Group enriched (lymphoid tissue, testis) |
Interactioni
Subunit structurei
Interacts with DLG1 and TP53.
2 PublicationsBinary interactionsi
Hide detailsQ96KB5
With | #Exp. | IntAct |
---|---|---|
ARAF [P10398] | 3 | EBI-536853,EBI-365961 |
DDIT4L [Q96D03] | 3 | EBI-536853,EBI-742054 |
MCRS1 [Q96EZ8] | 3 | EBI-536853,EBI-348259 |
TP53 [P04637] | 7 | EBI-536853,EBI-366083 |
ZNF526 [Q8TF50] | 3 | EBI-536853,EBI-11035148 |
Protein-protein interaction databases
BioGRIDi | 120971, 36 interactors |
IntActi | Q96KB5, 27 interactors |
MINTi | Q96KB5 |
STRINGi | 9606.ENSP00000428489 |
Chemistry databases
BindingDBi | Q96KB5 |
Miscellaneous databases
RNActi | Q96KB5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q96KB5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 322 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 291 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 320 – 322 | PDZ-interaction | 3 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0192, Eukaryota |
GeneTreei | ENSGT00720000108839 |
HOGENOMi | CLU_044128_1_0_1 |
InParanoidi | Q96KB5 |
OMAi | KMIMDIA |
OrthoDBi | 1030296at2759 |
PhylomeDBi | Q96KB5 |
TreeFami | TF329763 |
Family and domain databases
CDDi | cd14001, PKc_TOPK, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR041989, PKc_TOPK IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96KB5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEGISNFKTP SKLSEKKKSV LCSTPTINIP ASPFMQKLGF GTGVNVYLMK
60 70 80 90 100
RSPRGLSHSP WAVKKINPIC NDHYRSVYQK RLMDEAKILK SLHHPNIVGY
110 120 130 140 150
RAFTEANDGS LCLAMEYGGE KSLNDLIEER YKASQDPFPA AIILKVALNM
160 170 180 190 200
ARGLKYLHQE KKLLHGDIKS SNVVIKGDFE TIKICDVGVS LPLDENMTVT
210 220 230 240 250
DPEACYIGTE PWKPKEAVEE NGVITDKADI FAFGLTLWEM MTLSIPHINL
260 270 280 290 300
SNDDDDEDKT FDESDFDDEA YYAALGTRPP INMEELDESY QKVIELFSVC
310 320
TNEDPKDRPS AAHIVEALET DV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE5RFX4 | E5RFX4_HUMAN | Lymphokine-activated killer T-cell-... | PBK | 151 | Annotation score: | ||
E5RIE1 | E5RIE1_HUMAN | Lymphokine-activated killer T-cell-... | PBK | 63 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 34 | F → I in AAF71521 (PubMed:11378444).Curated | 1 | |
Sequence conflicti | 169 | K → E in BAB55019 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 254 | D → N in BAB55019 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021162 | 107 | N → S4 PublicationsCorresponds to variant dbSNP:rs3779620Ensembl. | 1 | |
Natural variantiVAR_051676 | 220 | E → D. Corresponds to variant dbSNP:rs17057901EnsemblClinVar. | 1 | |
Natural variantiVAR_041234 | 241 | M → L1 PublicationCorresponds to variant dbSNP:rs36086402Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055269 | 198 | T → TAPAFITILLVS in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB027249 mRNA Translation: BAA99576.1 AB027250 mRNA Translation: BAA99577.1 AF189722 mRNA Translation: AAF69107.1 AF237709 mRNA Translation: AAF71521.1 AK027291 mRNA Translation: BAB55019.1 AK301836 mRNA Translation: BAG63280.1 AC104997 Genomic DNA No translation available. CH471080 Genomic DNA Translation: EAW63536.1 CH471080 Genomic DNA Translation: EAW63537.1 BC015191 mRNA Translation: AAH15191.1 |
CCDSi | CCDS6063.1 [Q96KB5-1] CCDS64858.1 [Q96KB5-2] |
RefSeqi | NP_001265874.1, NM_001278945.1 [Q96KB5-2] NP_060962.2, NM_018492.3 [Q96KB5-1] XP_006716431.1, XM_006716368.2 |
Genome annotation databases
Ensembli | ENST00000301905; ENSP00000301905; ENSG00000168078 [Q96KB5-1] ENST00000522944; ENSP00000428489; ENSG00000168078 [Q96KB5-2] |
GeneIDi | 55872 |
KEGGi | hsa:55872 |
UCSCi | uc011lap.4, human [Q96KB5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB027249 mRNA Translation: BAA99576.1 AB027250 mRNA Translation: BAA99577.1 AF189722 mRNA Translation: AAF69107.1 AF237709 mRNA Translation: AAF71521.1 AK027291 mRNA Translation: BAB55019.1 AK301836 mRNA Translation: BAG63280.1 AC104997 Genomic DNA No translation available. CH471080 Genomic DNA Translation: EAW63536.1 CH471080 Genomic DNA Translation: EAW63537.1 BC015191 mRNA Translation: AAH15191.1 |
CCDSi | CCDS6063.1 [Q96KB5-1] CCDS64858.1 [Q96KB5-2] |
RefSeqi | NP_001265874.1, NM_001278945.1 [Q96KB5-2] NP_060962.2, NM_018492.3 [Q96KB5-1] XP_006716431.1, XM_006716368.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5J0A | X-ray | 2.74 | A | 23-320 | [»] | |
B | 19-319 | [»] | ||||
SMRi | Q96KB5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120971, 36 interactors |
IntActi | Q96KB5, 27 interactors |
MINTi | Q96KB5 |
STRINGi | 9606.ENSP00000428489 |
Chemistry databases
BindingDBi | Q96KB5 |
ChEMBLi | CHEMBL4896 |
GuidetoPHARMACOLOGYi | 2140 |
PTM databases
iPTMneti | Q96KB5 |
PhosphoSitePlusi | Q96KB5 |
Genetic variation databases
BioMutai | PBK |
DMDMi | 83305809 |
Proteomic databases
EPDi | Q96KB5 |
jPOSTi | Q96KB5 |
MassIVEi | Q96KB5 |
PaxDbi | Q96KB5 |
PeptideAtlasi | Q96KB5 |
PRIDEi | Q96KB5 |
ProteomicsDBi | 5416 77056 [Q96KB5-1] |
Protocols and materials databases
ABCDi | Q96KB5, 2 sequenced antibodies |
Antibodypediai | 1595, 558 antibodies |
DNASUi | 55872 |
Genome annotation databases
Ensembli | ENST00000301905; ENSP00000301905; ENSG00000168078 [Q96KB5-1] ENST00000522944; ENSP00000428489; ENSG00000168078 [Q96KB5-2] |
GeneIDi | 55872 |
KEGGi | hsa:55872 |
UCSCi | uc011lap.4, human [Q96KB5-1] |
Organism-specific databases
CTDi | 55872 |
DisGeNETi | 55872 |
GeneCardsi | PBK |
HGNCi | HGNC:18282, PBK |
HPAi | ENSG00000168078, Group enriched (lymphoid tissue, testis) |
MIMi | 611210, gene |
neXtProti | NX_Q96KB5 |
OpenTargetsi | ENSG00000168078 |
PharmGKBi | PA134925802 |
VEuPathDBi | HostDB:ENSG00000168078.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0192, Eukaryota |
GeneTreei | ENSGT00720000108839 |
HOGENOMi | CLU_044128_1_0_1 |
InParanoidi | Q96KB5 |
OMAi | KMIMDIA |
OrthoDBi | 1030296at2759 |
PhylomeDBi | Q96KB5 |
TreeFami | TF329763 |
Enzyme and pathway databases
PathwayCommonsi | Q96KB5 |
SignaLinki | Q96KB5 |
SIGNORi | Q96KB5 |
Miscellaneous databases
BioGRID-ORCSi | 55872, 7 hits in 1024 CRISPR screens |
ChiTaRSi | PBK, human |
GeneWikii | PBK_(gene) |
GenomeRNAii | 55872 |
Pharosi | Q96KB5, Tchem |
PROi | PR:Q96KB5 |
RNActi | Q96KB5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000168078, Expressed in ventricular zone and 147 other tissues |
ExpressionAtlasi | Q96KB5, baseline and differential |
Genevisiblei | Q96KB5, HS |
Family and domain databases
CDDi | cd14001, PKc_TOPK, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR041989, PKc_TOPK IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TOPK_HUMAN | |
Accessioni | Q96KB5Primary (citable) accession number: Q96KB5 Secondary accession number(s): B4DX68 Q9NZK6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | April 7, 2021 | |
This is version 175 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families