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Protein

1,5-anhydro-D-fructose reductase

Gene

AKR1E2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of 1,5-anhydro-D-fructose (AF) to 1,5-anhydro-D-glucitol (By similarity). Has low NADPH-dependent reductase activity towards 9,10-phenanthrenequinone (in vitro) (PubMed:12604216, PubMed:15118078).By similarity2 Publications

Catalytic activityi

1,5-anhydro-D-glucitol + NADP+ = 1,5-anhydro-D-fructose + NADPH.By similarity

Enzyme regulationi

Inhibited by p-chloromercuribenzoic acid and alkyliodines.By similarity

Kineticsi

  1. KM=6.1 µM for 9,10-phenanthrenequinone with NADPH as cofactor1 Publication
  2. KM=44.3 µM for 9,10-phenanthrenequinone (at 25 degrees Celsius)1 Publication
  3. KM=166 µM for NADPH (at 25 degrees Celsius)1 Publication
  4. KM=2.3 mM for NADH (at 25 degrees Celsius)1 Publication
  1. Vmax=0.42 nmol/min/mg enzyme towards 9,10-phenanthrenequinone with NADPH as cofactor1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADPBy similarity1
Active sitei40Proton donorBy similarity1
Sitei69Lowers pKa of active site TyrBy similarity1
Binding sitei102SubstrateBy similarity1
Binding sitei194NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi265 – 277NADPBy similarityAdd BLAST13

GO - Molecular functioni

  • 1,5-anhydro-D-fructose reductase activity Source: UniProtKB-EC
  • oxidoreductase activity Source: UniProtKB

GO - Biological processi

  • oxidation-reduction process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:HS15341-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
1,5-anhydro-D-fructose reductase (EC:1.1.1.263By similarity)
Short name:
AF reductase
Alternative name(s):
Aldo-keto reductase family 1 member C-like protein 2
Aldo-keto reductase family 1 member E2
LoopADR
Testis aldo-keto reductase1 Publication
Short name:
htAKR1 Publication
Testis-specific protein
Short name:
hTSP
Gene namesi
Name:AKR1E2
Synonyms:AKR1CL2, AKRDC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000165568.17
HGNCiHGNC:23437 AKR1E2
MIMi617451 gene
neXtProtiNX_Q96JD6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi83592
OpenTargetsiENSG00000165568
PharmGKBiPA165548224

Polymorphism and mutation databases

DMDMi269849539

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002878801 – 3201,5-anhydro-D-fructose reductaseAdd BLAST320

Proteomic databases

PaxDbiQ96JD6
PeptideAtlasiQ96JD6
PRIDEiQ96JD6
ProteomicsDBi76943
76944 [Q96JD6-2]
76945 [Q96JD6-3]
76946 [Q96JD6-4]
76947 [Q96JD6-5]

PTM databases

iPTMnetiQ96JD6
PhosphoSitePlusiQ96JD6

Expressioni

Tissue specificityi

Specifically expressed in testis (PubMed:12604216, PubMed:15118078). Expressed in testicular germ cells and testis interstitial cells (PubMed:15118078).2 Publications

Gene expression databases

BgeeiENSG00000165568
CleanExiHS_AKR1CL2
ExpressionAtlasiQ96JD6 baseline and differential
GenevisibleiQ96JD6 HS

Organism-specific databases

HPAiHPA037821
HPA037822

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9606.ENSP00000298375

Structurei

3D structure databases

ProteinModelPortaliQ96JD6
SMRiQ96JD6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577 Eukaryota
COG0656 LUCA
GeneTreeiENSGT00760000119041
HOGENOMiHOG000250272
HOVERGENiHBG000020
InParanoidiQ96JD6
KOiK13981
OMAiAYFYHNE
OrthoDBiEOG091G0D69
PhylomeDBiQ96JD6
TreeFamiTF106492

Family and domain databases

CDDicd06660 Aldo_ket_red, 1 hit
Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR018170 Aldo/ket_reductase_CS
IPR020471 Aldo/keto_reductase
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PANTHERiPTHR11732 PTHR11732, 1 hit
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 1 hit
PIRSFiPIRSF000097 AKR, 1 hit
PRINTSiPR00069 ALDKETRDTASE
SUPFAMiSSF51430 SSF51430, 1 hit
PROSITEiView protein in PROSITE
PS00798 ALDOKETO_REDUCTASE_1, 1 hit
PS00062 ALDOKETO_REDUCTASE_2, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96JD6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDIPAVGLS SWKASPGKVT EAVKEAIDAG YRHFDCAYFY HNEREVGAGI
60 70 80 90 100
RCKIKEGAVR REDLFIATKL WCTCHKKSLV ETACRKSLKA LKLNYLDLYL
110 120 130 140 150
IHWPMGFKPP HPEWIMSCSE LSFCLSHPRV QDLPLDESNM VIPSDTDFLD
160 170 180 190 200
TWEAMEDLVI TGLVKNIGVS NFNHEQLERL LNKPGLRFKP LTNQIECHPY
210 220 230 240 250
LTQKNLISFC QSRDVSVTAY RPLGGSCEGV DLIDNPVIKR IAKEHGKSPA
260 270 280 290 300
QILIRFQIQR NVIVIPGSIT PSHIKENIQV FDFELTQHDM DNILSLNRNL
310 320
RLAMFPITKN HKDYPFHIEY
Length:320
Mass (Da):36,589
Last modified:November 24, 2009 - v2
Checksum:iA73E06225E7F15DD
GO
Isoform 2 (identifier: Q96JD6-2) [UniParc]FASTAAdd to basket
Also known as: HTSP21 Publication, htAKR21 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     194-250: Missing.

Show »
Length:263
Mass (Da):30,323
Checksum:i024E146FABDEBA9F
GO
Isoform 3 (identifier: Q96JD6-3) [UniParc]FASTAAdd to basket
Also known as: HTSP11 Publication, htAKR11 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     194-250: Missing.
     307-307: I → M
     308-320: Missing.

Show »
Length:250
Mass (Da):28,667
Checksum:i241A3E8125FDE258
GO
Isoform 4 (identifier: Q96JD6-4) [UniParc]FASTAAdd to basket
Also known as: HTSP41 Publication, htAKR41 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     307-307: I → M
     308-320: Missing.

Show »
Length:307
Mass (Da):34,933
Checksum:i4DEEDE07568D2F03
GO
Isoform 5 (identifier: Q96JD6-5) [UniParc]FASTAAdd to basket
Also known as: HTSP31 Publication, htAKR31 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     154-251: Missing.

Show »
Length:222
Mass (Da):25,693
Checksum:i388A3D53CEA44BE4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312K → N in AAK58523 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03235652C → G. Corresponds to variant dbSNP:rs35429729Ensembl.1
Natural variantiVAR_03235786K → R. Corresponds to variant dbSNP:rs17133693Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025614154 – 251Missing in isoform 5. 1 PublicationAdd BLAST98
Alternative sequenceiVSP_025615194 – 250Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST57
Alternative sequenceiVSP_025616307I → M in isoform 3 and isoform 4. 2 Publications1
Alternative sequenceiVSP_025617308 – 320Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040820 mRNA Translation: BAC54565.1
AB040821 mRNA Translation: BAC54566.1
AB040822 mRNA Translation: BAC54567.1
AB055603 mRNA Translation: BAC54568.1
AF263242 mRNA Translation: AAK58523.1
AC091817 Genomic DNA No translation available.
BC002862 mRNA Translation: AAH02862.1
CCDSiCCDS31134.1 [Q96JD6-1]
CCDS59209.1 [Q96JD6-2]
CCDS59210.1 [Q96JD6-5]
RefSeqiNP_001035267.1, NM_001040177.2 [Q96JD6-1]
NP_001257950.1, NM_001271021.1 [Q96JD6-2]
NP_001257954.1, NM_001271025.1 [Q96JD6-5]
UniGeneiHs.657944

Genome annotation databases

EnsembliENST00000298375; ENSP00000298375; ENSG00000165568 [Q96JD6-1]
ENST00000334019; ENSP00000335034; ENSG00000165568 [Q96JD6-2]
ENST00000345253; ENSP00000335603; ENSG00000165568 [Q96JD6-5]
ENST00000463345; ENSP00000436794; ENSG00000165568 [Q96JD6-4]
ENST00000532248; ENSP00000432947; ENSG00000165568 [Q96JD6-3]
GeneIDi83592
KEGGihsa:83592
UCSCiuc001ihi.5 human [Q96JD6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAKCL2_HUMAN
AccessioniPrimary (citable) accession number: Q96JD6
Secondary accession number(s): Q86Z16
, Q86Z17, Q86Z18, Q9BU71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: November 24, 2009
Last modified: June 20, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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