UniProtKB - Q96JB5 (CK5P3_HUMAN)
CDK5 regulatory subunit-associated protein 3
CDK5RAP3
Functioni
GO - Molecular functioni
- cyclin binding Source: UniProtKB
- MDM2/MDM4 family protein binding Source: UniProtKB
- mitogen-activated protein kinase binding Source: UniProtKB
- NF-kappaB binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
- ubiquitin-like protein ligase binding Source: MGI
GO - Biological processi
- apoptotic nuclear changes Source: UniProtKB
- brain development Source: UniProtKB
- cell population proliferation Source: UniProtKB
- definitive erythrocyte differentiation Source: UniProtKB
- endoplasmic reticulum unfolded protein response Source: UniProtKB
- liver development Source: UniProtKB
- mitotic G2/M transition checkpoint Source: UniProtKB
- mitotic G2 DNA damage checkpoint Source: UniProtKB
- negative regulation of cellular protein catabolic process Source: UniProtKB
- negative regulation of MAP kinase activity Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- negative regulation of protein kinase activity by regulation of protein phosphorylation Source: UniProtKB
- negative regulation of protein phosphorylation Source: UniProtKB
- negative regulation of protein serine/threonine kinase activity Source: UniProtKB
- positive regulation of protein localization to nucleus Source: UniProtKB
- positive regulation of protein ubiquitination Source: UniProtKB
- positive regulation of signal transduction by p53 class mediator Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: Ensembl
- protein ufmylation Source: MGI
- regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
- regulation of mitotic cell cycle Source: GO_Central
- regulation of neuron differentiation Source: UniProtKB
- regulation of phosphatase activity Source: UniProtKB
- response to endoplasmic reticulum stress Source: UniProtKB
- viral process Source: UniProtKB-KW
Keywordsi
Biological process | Host-virus interaction |
Enzyme and pathway databases
PathwayCommonsi | Q96JB5 |
Names & Taxonomyi
Protein namesi | Recommended name: CDK5 regulatory subunit-associated protein 3CuratedAlternative name(s): CDK5 activator-binding protein C531 Publication LXXLL/leucine-zipper-containing ARF-binding protein1 Publication Protein HSF-27Imported |
Gene namesi | ORF Names:MSTP016, OK/SW-cl.114, PP1553 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18673, CDK5RAP3 |
MIMi | 608202, gene |
neXtProti | NX_Q96JB5 |
VEuPathDBi | HostDB:ENSG00000108465.14 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytoskeleton
- centrosome 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 3 Publications
Note: Colocalizes and associates with microtubules.1 Publication
Cytoskeleton
- centrosome Source: UniProtKB
Cytosol
- cytosol Source: HPA
Nucleus
Other locations
- cytoplasm Source: UniProtKB
- intracellular membrane-bounded organelle Source: HPA
- membrane Source: UniProtKB
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 268 | D → E: Alters cleavage by CASP3 in vitro. Prevents apoptosis-induced cleavage in vivo; when associated with E-282 and E-311. 1 Publication | 1 | |
Mutagenesisi | 282 | D → E: Alters cleavage by CASP3 in vitro. Prevents apoptosis-induced cleavage in vivo; when associated with E-268 and E-311. 1 Publication | 1 | |
Mutagenesisi | 311 | D → E: Alters cleavage by CASP3 in vitro. Prevents apoptosis-induced cleavage in vivo; when associated with E-268 and E-282. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 80279 |
OpenTargetsi | ENSG00000108465 |
PharmGKBi | PA38633 |
Miscellaneous databases
Pharosi | Q96JB5, Tbio |
Genetic variation databases
BioMutai | CDK5RAP3 |
DMDMi | 32129444 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000220516 | 1 – 506 | CDK5 regulatory subunit-associated protein 3Add BLAST | 506 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 450 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q96JB5 |
jPOSTi | Q96JB5 |
MassIVEi | Q96JB5 |
MaxQBi | Q96JB5 |
PaxDbi | Q96JB5 |
PeptideAtlasi | Q96JB5 |
PRIDEi | Q96JB5 |
ProteomicsDBi | 26284 76930 [Q96JB5-1] 76931 [Q96JB5-2] 76932 [Q96JB5-3] |
PTM databases
iPTMneti | Q96JB5 |
PhosphoSitePlusi | Q96JB5 |
SwissPalmi | Q96JB5 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000108465, Expressed in pituitary gland and 236 other tissues |
ExpressionAtlasi | Q96JB5, baseline and differential |
Genevisiblei | Q96JB5, HS |
Organism-specific databases
HPAi | ENSG00000108465, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with CDK5R1; competes with CDK5RAP1 and CDK5RAP2 (PubMed:12737517).
Interacts with RELA (PubMed:17785205).
Interacts with CHEK1; may negatively regulate CHEK1 and thereby stimulate entry into mitosis (PubMed:19223857).
Interacts with CDKN2A/ARF and MDM2; forms a ternary complex involved in regulation of p53/TP53 (PubMed:16173922).
Interacts with UFL1; the interaction is direct (PubMed:20164180, PubMed:20228063). Interaction with UFL1 anchors CDK5RAP3 in the cytoplasm, preventing its translocation to the nucleus which allows expression of the CCND1 cyclin and progression of cells through the G1/S transition (By similarity).
Interacts with DDRGK1 (PubMed:20228063).
Interacts with MAPK14 (PubMed:21283629).
Interacts with CCNB1 (PubMed:15790566).
Interacts with TUBG1; may regulate CDK5RAP3 in mitotic G2/M transition checkpoint (PubMed:21465471).
By similarity9 Publications(Microbial infection) Interacts with hepatitis B virus large envelope protein mutant pre-s2; promotes mitotic entry.
1 PublicationBinary interactionsi
Hide detailsQ96JB5
With | #Exp. | IntAct |
---|---|---|
DISC1 [Q9NRI5] | 3 | EBI-718818,EBI-529989 |
FKBP6 [O75344] | 12 | EBI-718818,EBI-744771 |
RELA [Q04206] | 4 | EBI-718818,EBI-73886 |
TSPYL4 [Q9UJ04] | 6 | EBI-718818,EBI-308511 |
UFL1 [O94874] | 3 | EBI-718818,EBI-1048088 |
GO - Molecular functioni
- cyclin binding Source: UniProtKB
- MDM2/MDM4 family protein binding Source: UniProtKB
- mitogen-activated protein kinase binding Source: UniProtKB
- NF-kappaB binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
- ubiquitin-like protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 123213, 83 interactors |
IntActi | Q96JB5, 45 interactors |
MINTi | Q96JB5 |
STRINGi | 9606.ENSP00000438886 |
Miscellaneous databases
RNActi | Q96JB5, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 269 – 506 | Required for interaction with UFL1 and mediates interaction with CHEK12 PublicationsAdd BLAST | 238 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2607, Eukaryota |
GeneTreei | ENSGT00390000000713 |
HOGENOMi | CLU_025645_1_0_1 |
InParanoidi | Q96JB5 |
OMAi | PGVRKQM |
OrthoDBi | 619606at2759 |
PhylomeDBi | Q96JB5 |
Family and domain databases
InterProi | View protein in InterPro IPR008491, CDK5RAP3 |
PANTHERi | PTHR14894, PTHR14894, 1 hit |
Pfami | View protein in Pfam PF05600, DUF773, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 12 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEDHQHVPID IQTSKLLDWL VDRRHCSLKW QSLVLTIREK INAAIQDMPE
60 70 80 90 100
SEEIAQLLSG SYIHYFHCLR ILDLLKGTEA STKNIFGRYS SQRMKDWQEI
110 120 130 140 150
IALYEKDNTY LVELSSLLVR NVNYEIPSLK KQIAKCQQLQ QEYSRKEEEC
160 170 180 190 200
QAGAAEMREQ FYHSCKQYGI TGENVRGELL ALVKDLPSQL AEIGAAAQQS
210 220 230 240 250
LGEAIDVYQA SVGFVCESPT EQVLPMLRFV QKRGNSTVYE WRTGTEPSVV
260 270 280 290 300
ERPHLEELPE QVAEDAIDWG DFGVEAVSEG TDSGISAEAA GIDWGIFPES
310 320 330 340 350
DSKDPGGDGI DWGDDAVALQ ITVLEAGTQA PEGVARGPDA LTLLEYTETR
360 370 380 390 400
NQFLDELMEL EIFLAQRAVE LSEEADVLSV SQFQLAPAIL QGQTKEKMVT
410 420 430 440 450
MVSVLEDLIG KLTSLQLQHL FMILASPRYV DRVTEFLQQK LKQSQLLALK
460 470 480 490 500
KELMVQKQQE ALEEQAALEP KLDLLLEKTK ELQKLIEADI SKRYSGRPVN
LMGTSL
Computationally mapped potential isoform sequencesi
There are 12 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3QRM1 | J3QRM1_HUMAN | CDK5 regulatory subunit-associated ... | CDK5RAP3 | 272 | Annotation score: | ||
J3QS62 | J3QS62_HUMAN | CDK5 regulatory subunit-associated ... | CDK5RAP3 | 196 | Annotation score: | ||
J3QL86 | J3QL86_HUMAN | CDK5 regulatory subunit-associated ... | CDK5RAP3 | 123 | Annotation score: | ||
J3QQY1 | J3QQY1_HUMAN | CDK5 regulatory subunit-associated ... | CDK5RAP3 | 281 | Annotation score: | ||
J3QRX0 | J3QRX0_HUMAN | CDK5 regulatory subunit-associated ... | CDK5RAP3 | 110 | Annotation score: | ||
J3KS63 | J3KS63_HUMAN | CDK5 regulatory subunit-associated ... | CDK5RAP3 | 124 | Annotation score: | ||
J3KRI2 | J3KRI2_HUMAN | CDK5 regulatory subunit-associated ... | CDK5RAP3 | 105 | Annotation score: | ||
J3KRK4 | J3KRK4_HUMAN | CDK5 regulatory subunit-associated ... | CDK5RAP3 | 118 | Annotation score: | ||
J3KTA5 | J3KTA5_HUMAN | CDK5 regulatory subunit-associated ... | CDK5RAP3 | 64 | Annotation score: | ||
J3QQS7 | J3QQS7_HUMAN | CDK5 regulatory subunit-associated ... | CDK5RAP3 | 58 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 98 | Q → R in BAH13320 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 113 | E → V in BAD97107 (Ref. 6) Curated | 1 | |
Sequence conflicti | 406 | E → K in AAK69655 (Ref. 3) Curated | 1 | |
Sequence conflicti | 443 | Q → E in BAD97107 (Ref. 6) Curated | 1 | |
Sequence conflicti | 479 | T → A in BAH13320 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048688 | 324 | L → V. Corresponds to variant dbSNP:rs35054799Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007568 | 1 – 225 | Missing in isoform 3. 1 PublicationAdd BLAST | 225 | |
Alternative sequenceiVSP_007566 | 1 – 87 | Missing in isoform 2. 1 PublicationAdd BLAST | 87 | |
Alternative sequenceiVSP_055646 | 1 – 2 | ME → MRRQSMTSATRDLHTALDGK ATQGGKK in isoform 4. 1 Publication | 2 | |
Alternative sequenceiVSP_007567 | 88 – 111 | RYSSQ…DNTYL → MCVHPGACLPHVGVSWAEFP GHFS in isoform 2. 1 PublicationAdd BLAST | 24 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000338399; ENSP00000344683; ENSG00000108465 [Q96JB5-1] ENST00000536708; ENSP00000438886; ENSG00000108465 [Q96JB5-4] |
GeneIDi | 80279 |
KEGGi | hsa:80279 |
UCSCi | uc002imr.5, human [Q96JB5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
SMRi | Q96JB5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 123213, 83 interactors |
IntActi | Q96JB5, 45 interactors |
MINTi | Q96JB5 |
STRINGi | 9606.ENSP00000438886 |
PTM databases
iPTMneti | Q96JB5 |
PhosphoSitePlusi | Q96JB5 |
SwissPalmi | Q96JB5 |
Genetic variation databases
BioMutai | CDK5RAP3 |
DMDMi | 32129444 |
Proteomic databases
EPDi | Q96JB5 |
jPOSTi | Q96JB5 |
MassIVEi | Q96JB5 |
MaxQBi | Q96JB5 |
PaxDbi | Q96JB5 |
PeptideAtlasi | Q96JB5 |
PRIDEi | Q96JB5 |
ProteomicsDBi | 26284 76930 [Q96JB5-1] 76931 [Q96JB5-2] 76932 [Q96JB5-3] |
Protocols and materials databases
Antibodypediai | 8591, 217 antibodies |
DNASUi | 80279 |
Genome annotation databases
Ensembli | ENST00000338399; ENSP00000344683; ENSG00000108465 [Q96JB5-1] ENST00000536708; ENSP00000438886; ENSG00000108465 [Q96JB5-4] |
GeneIDi | 80279 |
KEGGi | hsa:80279 |
UCSCi | uc002imr.5, human [Q96JB5-1] |
Organism-specific databases
CTDi | 80279 |
DisGeNETi | 80279 |
GeneCardsi | CDK5RAP3 |
HGNCi | HGNC:18673, CDK5RAP3 |
HPAi | ENSG00000108465, Low tissue specificity |
MIMi | 608202, gene |
neXtProti | NX_Q96JB5 |
OpenTargetsi | ENSG00000108465 |
PharmGKBi | PA38633 |
VEuPathDBi | HostDB:ENSG00000108465.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2607, Eukaryota |
GeneTreei | ENSGT00390000000713 |
HOGENOMi | CLU_025645_1_0_1 |
InParanoidi | Q96JB5 |
OMAi | PGVRKQM |
OrthoDBi | 619606at2759 |
PhylomeDBi | Q96JB5 |
Enzyme and pathway databases
PathwayCommonsi | Q96JB5 |
Miscellaneous databases
BioGRID-ORCSi | 80279, 47 hits in 1005 CRISPR screens |
ChiTaRSi | CDK5RAP3, human |
GeneWikii | CDK5RAP3 |
GenomeRNAii | 80279 |
Pharosi | Q96JB5, Tbio |
PROi | PR:Q96JB5 |
RNActi | Q96JB5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000108465, Expressed in pituitary gland and 236 other tissues |
ExpressionAtlasi | Q96JB5, baseline and differential |
Genevisiblei | Q96JB5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR008491, CDK5RAP3 |
PANTHERi | PTHR14894, PTHR14894, 1 hit |
Pfami | View protein in Pfam PF05600, DUF773, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CK5P3_HUMAN | |
Accessioni | Q96JB5Primary (citable) accession number: Q96JB5 Secondary accession number(s): B7Z6N4 Q9HBR9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 16, 2003 |
Last sequence update: | June 16, 2003 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families