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UniProtKB - Q96J02 (ITCH_HUMAN)

Protein

E3 ubiquitin-protein ligase Itchy homolog

Gene

ITCH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:14602072, PubMed:17028573, PubMed:16387660, PubMed:18718448, PubMed:18718449, PubMed:11046148, PubMed:19592251, PubMed:19116316, PubMed:19881509, PubMed:20491914, PubMed:20392206, PubMed:20068034, PubMed:23146885, PubMed:24790097, PubMed:25631046). Catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation (PubMed:17028573, PubMed:18718448, PubMed:19131965, PubMed:19881509). Involved in the control of inflammatory signaling pathways (PubMed:19131965). Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways (PubMed:19131965). Promotes the association of the complex after TNF stimulation (PubMed:19131965). Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains (PubMed:19131965). This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1 (PubMed:19131965). Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways (PubMed:19592251). Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response (PubMed:18718448, PubMed:20491914). Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages (PubMed:18718448). Mediates JUN ubiquitination and degradation (By similarity). Mediates JUNB ubiquitination and degradation (PubMed:16387660). Critical regulator of type 2 helper T (Th2) cell cytokine production by inducing JUNB ubiquitination and degradation (By similarity). Involved in the negative regulation of MAVS-dependent cellular antiviral responses (PubMed:19881509). Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation (PubMed:19881509). Following ligand stimulation, regulates sorting of Wnt receptor FZD4 to the degradative endocytic pathway probably by modulating PI42KA activity (PubMed:23146885). Ubiquitinates PI4K2A and negatively regulates its catalytic activity (PubMed:23146885). Ubiquitinates chemokine receptor CXCR4 and regulates sorting of CXCR4 to the degradative endocytic pathway following ligand stimulation by ubiquitinating endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PubMed:14602072, PubMed:23146885). Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination (PubMed:17028573, PubMed:18628966, PubMed:23886940). Ubiquitinates SNX9 (PubMed:20491914). Ubiquitinates MAP3K7 through 'Lys-48'-linked conjugation (By similarity). Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP (PubMed:20068034). Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID (PubMed:20392206). Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).By similarity17 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.15 Publications

Activity regulationi

Activated by NDFIP1- and NDFIP2-binding (PubMed:25631046). Activated by PI4K2A-binding (PubMed:23146885). Inhibited by DTX3L-binding (PubMed:24790097). Inhibited by N4BP1 binding (By similarity).By similarity3 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.15 Publications
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei871Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionTransferase
Biological processAntiviral defense, Apoptosis, Host-virus interaction, Immunity, Innate immunity, Ubl conjugation pathway

Enzyme and pathway databases

BRENDAi6.3.2.19 2681
ReactomeiR-HSA-1253288 Downregulation of ERBB4 signaling
R-HSA-168638 NOD1/2 Signaling Pathway
R-HSA-2122948 Activated NOTCH1 Transmits Signal to the Nucleus
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ96J02
SIGNORiQ96J02
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Itchy homolog (EC:2.3.2.267 Publications)
Short name:
Itch
Alternative name(s):
Atrophin-1-interacting protein 4
Short name:
AIP4
HECT-type E3 ubiquitin transferase Itchy homolog
NFE2-associated polypeptide 1
Short name:
NAPP1
Gene namesi
Name:ITCH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000078747.12
HGNCiHGNC:13890 ITCH
MIMi606409 gene
neXtProtiNX_Q96J02

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endosome, Membrane, Nucleus

Pathology & Biotechi

Involvement in diseasei

Autoimmune disease, multisystem, with facial dysmorphism (ADMFD)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features and autoimmune inflammatory cell infiltration of the lungs, liver and gut.
See also OMIM:613385

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi343Y → F: No effect on phosphorylation on T-cell stimulation nor in the presence of FYN. 1 Publication1
Mutagenesisi420Y → F: Greatly reduced phosphorylation on T-cell stimulation and in the presence of FYN. Increased ITCH-mediated Ub conjugation and degradation of JUNB. 1 Publication1
Mutagenesisi455Y → F: No effect on phosphorylation on T-cell stimulation nor in the presence of FYN. 1 Publication1
Mutagenesisi871C → A: Loss of ubiquitin protein ligase activity. Results in altered endosomal sorting and reduced degradation of CXCR4. Unable to inhibit MAVS-induced activation of INFB. 5 Publications1

Organism-specific databases

DisGeNETi83737
MalaCardsiITCH
MIMi613385 phenotype
OpenTargetsiENSG00000078747
Orphaneti228426 Syndromic multisystem autoimmune disease due to Itch deficiency
PharmGKBiPA29934

Polymorphism and mutation databases

BioMutaiITCH
DMDMi37537897

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001203172 – 903E3 ubiquitin-protein ligase Itchy homologAdd BLAST902

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei240Phosphoserine; by MAPK8By similarity1
Modified residuei263Phosphothreonine; by MAPK8By similarity1
Modified residuei273Phosphoserine; by MAPK8By similarity1
Modified residuei385Phosphothreonine; by SGK31 Publication1
Modified residuei420Phosphotyrosine; by FYN1 Publication1
Modified residuei450Phosphoserine; by SGK31 Publication1

Post-translational modificationi

On T-cell activation, phosphorylation by the JNK cascade on serine and threonine residues surrounding the PRR domain accelerates the ubiquitination and degradation of JUN and JUNB. The increased ITCH catalytic activity due to phosphorylation by JNK1 may occur due to a conformational change disrupting the interaction between the PRR/WW motifs domain and the HECT domain and, thus exposing the HECT domain (By similarity). Phosphorylation by FYN reduces interaction with JUNB and negatively controls JUN ubiquitination and degradation.By similarity4 Publications
Monoubiquitinated (PubMed:19116316). Autopolyubiquitinated with 'Lys-63' linkages which does not lead to protein degradation (PubMed:18718449, PubMed:23146885, PubMed:24790097).4 Publications

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96J02
MaxQBiQ96J02
PaxDbiQ96J02
PeptideAtlasiQ96J02
PRIDEiQ96J02
ProteomicsDBi76882
76883 [Q96J02-2]

PTM databases

iPTMnetiQ96J02
PhosphoSitePlusiQ96J02

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSG00000078747 Expressed in 217 organ(s), highest expression level in sperm
CleanExiHS_ITCH
ExpressionAtlasiQ96J02 baseline and differential
GenevisibleiQ96J02 HS

Organism-specific databases

HPAiHPA021126
HPA049032

Interactioni

Subunit structurei

Monomer. Interacts (via WW domains) with OCNL (By similarity). Interacts (via WW domains) with NOTCH1 (By similarity). Interacts (via WW domains) with JUN (By similarity). Interacts with JUNB; the interaction promotes ITCH-mediated ubiquitination and degradation of JUNB (PubMed:16387660). Interacts with FYN; the interaction phosphorylates ITCH on Tyr-420 decreasing binding of JUNB (PubMed:16387660). Interacts (via WW domain 2) with N4BP1; the interaction inhibits the E3 ubiquitin-protein ligase activity (By similarity). Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase and may induce its recruitment to exosomes (By similarity). Interacts with ARHGEF7 (PubMed:17652093). Interacts with RNF11 (PubMed:14559117, PubMed:19131965). Interacts (via the WW 1 domain) with NFE2 (via the PXY motif 1); the interaction promotes 'Lys-63'-linked ubiquitination of NFE2, retains it in the cytoplasm and prevents its transactivation activity (PubMed:11318614, PubMed:18718448). Interacts (via WW domains) with CXCR4 (via C-terminus); the interaction depends on CXCR4 phosphorylation (PubMed:19116316). Found in a complex with E3 ligase DTX3L and ESCRT-0 components HGS and STAM (PubMed:24790097). Interacts with DTX3L (via C-terminus); the interaction is increased upon CXCL12 stimulation and inhibits ITCH catalytic activity (the interaction is direct) (PubMed:24790097). Interacts with HGS (PubMed:14602072). Interacts (via WW domains) with PCBP2 within a complex containing ITCH, MAVS and PCBP2 (PubMed:19881509). Interacts (via WW domains) with TXNIP (via C-terminus) (PubMed:20068034). Interacts with p15 BID (PubMed:20392206). Interacts with ERBB4 (PubMed:20858735). Interacts with DTX1 (PubMed:17028573). Interacts with SPART (PubMed:19580544). Interacts with SNX9 and SNX18 (PubMed:20491914). Interacts (via its WW domains) with ATN1 (PubMed:9647693). Interacts (via WW domains) with SGK3 (PubMed:16888620). Interacts with CBLC (PubMed:12226085). Interacts with OTUD7B (PubMed:22179831). Interacts (via WW domain 1,2 and 3) with PI4K2A; the interaction inhibits PI4K2A catalytic activity and promotes ITCH catalytic activity (PubMed:23146885). Interacts with ARRDC4 (PubMed:23236378). Part of a complex containing ITCH, NDFIP1 and MAP3K7 (By similarity). Interacts with UBE2L3; the interaction is mediated by NDFIP1 (PubMed:25632008). Interacts with MAPK8/JNK1 (By similarity). Interacts (via WW domains) with ARRDC1 (via PPxY motifs); the interaction is direct and participates to the recruitment of the ubiquitin-protein ligase ITCH to the NOTCH1 receptor (PubMed:21191027, PubMed:23886940). Interacts (via WW domains) with ARRDC2 (PubMed:21191027). Interacts (via WW domains) with ARRDC3 (PubMed:21191027, PubMed:23886940).By similarity25 Publications
(Microbial infection) Interacts with Epstein-Barr virus LMP2A.1 Publication
(Microbial infection) Interacts with Human cytomegalovirus (HCMV) protein UL42; this interaction induces ubiquitination and degradation of ITCH.1 Publication
(Microbial infection) Interacts with Herpes simplex virus 2 protein UL56.1 Publication
(Microbial infection) Interacts with ebola virus protein VP40; this interaction is required for efficient viral egress from the infected cell.1 Publication

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi123747, 208 interactors
CORUMiQ96J02
DIPiDIP-29849N
ELMiQ96J02
IntActiQ96J02, 40 interactors
MINTiQ96J02
STRINGi9606.ENSP00000363998

Structurei

Secondary structure

1903
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96J02
SMRiQ96J02
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96J02

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 99C2PROSITE-ProRule annotationAdd BLAST95
Domaini326 – 359WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini358 – 391WW 2PROSITE-ProRule annotationAdd BLAST34
Domaini438 – 471WW 3PROSITE-ProRule annotationAdd BLAST34
Domaini478 – 511WW 4PROSITE-ProRule annotationAdd BLAST34
Domaini569 – 903HECTPROSITE-ProRule annotationAdd BLAST335

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni395 – 471Required for interaction with FYN1 PublicationAdd BLAST77
Regioni574 – 583MAP kinase docking siteBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi252 – 267Arg/Pro-rich (PRR domain)Add BLAST16

Domaini

The WW domains mediate interaction with PPxY motif-containing proteins.1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0940 Eukaryota
COG5021 LUCA
GeneTreeiENSGT00760000118966
HOVERGENiHBG004134
InParanoidiQ96J02
KOiK05632
OMAiETRVNTN
OrthoDBiEOG091G0SS8
PhylomeDBiQ96J02
TreeFamiTF323658

Family and domain databases

CDDicd00078 HECTc, 1 hit
cd00201 WW, 4 hits
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR024928 E3_ub_ligase_SMURF1
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00632 HECT, 1 hit
PF00397 WW, 4 hits
PIRSFiPIRSF001569 E3_ub_ligase_SMURF1, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00119 HECTc, 1 hit
SM00456 WW, 4 hits
SUPFAMiSSF51045 SSF51045, 4 hits
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50237 HECT, 1 hit
PS01159 WW_DOMAIN_1, 4 hits
PS50020 WW_DOMAIN_2, 4 hits

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96J02-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSDSGSQLGS MGSLTMKSQL QITVISAKLK ENKKNWFGPS PYVEVTVDGQ 
        60         70         80         90        100
SKKTEKCNNT NSPKWKQPLT VIVTPVSKLH FRVWSHQTLK SDVLLGTAAL 
       110        120        130        140        150
DIYETLKSNN MKLEEVVVTL QLGGDKEPTE TIGDLSICLD GLQLESEVVT 
       160        170        180        190        200
NGETTCSENG VSLCLPRLEC NSAISAHCNL CLPGLSDSPI SASRVAGFTG 
       210        220        230        240        250
ASQNDDGSRS KDETRVSTNG SDDPEDAGAG ENRRVSGNNS PSLSNGGFKP 
       260        270        280        290        300
SRPPRPSRPP PPTPRRPASV NGSPSATSES DGSSTGSLPP TNTNTNTSEG 
       310        320        330        340        350
ATSGLIIPLT ISGGSGPRPL NPVTQAPLPP GWEQRVDQHG RVYYVDHVEK 
       360        370        380        390        400
RTTWDRPEPL PPGWERRVDN MGRIYYVDHF TRTTTWQRPT LESVRNYEQW 
       410        420        430        440        450
QLQRSQLQGA MQQFNQRFIY GNQDLFATSQ SKEFDPLGPL PPGWEKRTDS 
       460        470        480        490        500
NGRVYFVNHN TRITQWEDPR SQGQLNEKPL PEGWEMRFTV DGIPYFVDHN 
       510        520        530        540        550
RRTTTYIDPR TGKSALDNGP QIAYVRDFKA KVQYFRFWCQ QLAMPQHIKI 
       560        570        580        590        600
TVTRKTLFED SFQQIMSFSP QDLRRRLWVI FPGEEGLDYG GVAREWFFLL 
       610        620        630        640        650
SHEVLNPMYC LFEYAGKDNY CLQINPASYI NPDHLKYFRF IGRFIAMALF 
       660        670        680        690        700
HGKFIDTGFS LPFYKRILNK PVGLKDLESI DPEFYNSLIW VKENNIEECD 
       710        720        730        740        750
LEMYFSVDKE ILGEIKSHDL KPNGGNILVT EENKEEYIRM VAEWRLSRGV 
       760        770        780        790        800
EEQTQAFFEG FNEILPQQYL QYFDAKELEV LLCGMQEIDL NDWQRHAIYR 
       810        820        830        840        850
HYARTSKQIM WFWQFVKEID NEKRMRLLQF VTGTCRLPVG GFADLMGSNG 
       860        870        880        890        900
PQKFCIEKVG KENWLPRSHT CFNRLDLPPY KSYEQLKEKL LFAIEETEGF 

GQE
Note: No experimental confirmation available.
Length:903
Mass (Da):102,803
Last modified:October 3, 2003 - v2
Checksum:i6777A2043C7B67BC
GO
Isoform 2 (identifier: Q96J02-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     159-200: NGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTG → S

Show »
Length:862
Mass (Da):98,676
Checksum:iA3D960E7F4DBF9D3
GO
Isoform 3 (identifier: Q96J02-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-110: Missing.
     159-200: NGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTG → S

Note: No experimental confirmation available.
Show »
Length:752
Mass (Da):86,498
Checksum:iB08AB68B285391F8
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2PNZ8A0A1W2PNZ8_HUMAN
E3 ubiquitin-protein ligase Itchy h...
ITCH
37Annotation score:
A0A1W2PR79A0A1W2PR79_HUMAN
E3 ubiquitin-protein ligase Itchy h...
ITCH
158Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti297T → I in BAG64996 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0447321 – 110Missing in isoform 3. 1 PublicationAdd BLAST110
Alternative sequenceiVSP_008451159 – 200NGVSL…AGFTG → S in isoform 2 and isoform 3. 5 PublicationsAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095745 mRNA Translation: AAK39399.1
AB056663 mRNA Translation: BAB39389.1
AK304090 mRNA Translation: BAG64996.1
AK315212 mRNA Translation: BAG37647.1
AL109923 Genomic DNA No translation available.
AL356299 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76272.1
CH471077 Genomic DNA Translation: EAW76274.1
CH471077 Genomic DNA Translation: EAW76276.1
BC006848 mRNA Translation: AAH06848.1
BC011571 mRNA Translation: AAH11571.1
AF038564 mRNA Translation: AAC04845.1
CCDSiCCDS13234.1 [Q96J02-2]
CCDS58768.1 [Q96J02-1]
CCDS58769.1 [Q96J02-3]
RefSeqiNP_001244066.1, NM_001257137.2 [Q96J02-1]
NP_001244067.1, NM_001257138.2 [Q96J02-3]
NP_001311126.1, NM_001324197.1 [Q96J02-1]
NP_001311127.1, NM_001324198.1 [Q96J02-2]
NP_113671.3, NM_031483.6 [Q96J02-2]
XP_016883578.1, XM_017028089.1 [Q96J02-1]
XP_016883580.1, XM_017028091.1 [Q96J02-3]
UniGeneiHs.632272

Genome annotation databases

EnsembliENST00000262650; ENSP00000262650; ENSG00000078747 [Q96J02-1]
ENST00000374864; ENSP00000363998; ENSG00000078747 [Q96J02-2]
ENST00000535650; ENSP00000445608; ENSG00000078747 [Q96J02-3]
GeneIDi83737
KEGGihsa:83737
UCSCiuc002xak.3 human [Q96J02-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095745 mRNA Translation: AAK39399.1
AB056663 mRNA Translation: BAB39389.1
AK304090 mRNA Translation: BAG64996.1
AK315212 mRNA Translation: BAG37647.1
AL109923 Genomic DNA No translation available.
AL356299 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76272.1
CH471077 Genomic DNA Translation: EAW76274.1
CH471077 Genomic DNA Translation: EAW76276.1
BC006848 mRNA Translation: AAH06848.1
BC011571 mRNA Translation: AAH11571.1
AF038564 mRNA Translation: AAC04845.1
CCDSiCCDS13234.1 [Q96J02-2]
CCDS58768.1 [Q96J02-1]
CCDS58769.1 [Q96J02-3]
RefSeqiNP_001244066.1, NM_001257137.2 [Q96J02-1]
NP_001244067.1, NM_001257138.2 [Q96J02-3]
NP_001311126.1, NM_001324197.1 [Q96J02-1]
NP_001311127.1, NM_001324198.1 [Q96J02-2]
NP_113671.3, NM_031483.6 [Q96J02-2]
XP_016883578.1, XM_017028089.1 [Q96J02-1]
XP_016883580.1, XM_017028091.1 [Q96J02-3]
UniGeneiHs.632272

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DMVNMR-A328-357[»]
2KYKNMR-A359-392[»]
2NQ3X-ray1.80A1-155[»]
2P4RX-ray2.00T246-270[»]
2YSFNMR-A480-512[»]
3TUGX-ray2.27A524-903[»]
4ROFX-ray2.03A/B436-474[»]
5C7MX-ray3.03A524-899[»]
5CQ2X-ray1.40A433-521[»]
5DWSX-ray1.65A/C/E/G436-474[»]
5DZDX-ray1.57A/B475-514[»]
5SXPX-ray1.65F/G249-269[»]
ProteinModelPortaliQ96J02
SMRiQ96J02
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123747, 208 interactors
CORUMiQ96J02
DIPiDIP-29849N
ELMiQ96J02
IntActiQ96J02, 40 interactors
MINTiQ96J02
STRINGi9606.ENSP00000363998

PTM databases

iPTMnetiQ96J02
PhosphoSitePlusiQ96J02

Polymorphism and mutation databases

BioMutaiITCH
DMDMi37537897

Proteomic databases

EPDiQ96J02
MaxQBiQ96J02
PaxDbiQ96J02
PeptideAtlasiQ96J02
PRIDEiQ96J02
ProteomicsDBi76882
76883 [Q96J02-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262650; ENSP00000262650; ENSG00000078747 [Q96J02-1]
ENST00000374864; ENSP00000363998; ENSG00000078747 [Q96J02-2]
ENST00000535650; ENSP00000445608; ENSG00000078747 [Q96J02-3]
GeneIDi83737
KEGGihsa:83737
UCSCiuc002xak.3 human [Q96J02-1]

Organism-specific databases

CTDi83737
DisGeNETi83737
EuPathDBiHostDB:ENSG00000078747.12
GeneCardsiITCH
H-InvDBiHIX0015745
HGNCiHGNC:13890 ITCH
HPAiHPA021126
HPA049032
MalaCardsiITCH
MIMi606409 gene
613385 phenotype
neXtProtiNX_Q96J02
OpenTargetsiENSG00000078747
Orphaneti228426 Syndromic multisystem autoimmune disease due to Itch deficiency
PharmGKBiPA29934
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0940 Eukaryota
COG5021 LUCA
GeneTreeiENSGT00760000118966
HOVERGENiHBG004134
InParanoidiQ96J02
KOiK05632
OMAiETRVNTN
OrthoDBiEOG091G0SS8
PhylomeDBiQ96J02
TreeFamiTF323658

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi6.3.2.19 2681
ReactomeiR-HSA-1253288 Downregulation of ERBB4 signaling
R-HSA-168638 NOD1/2 Signaling Pathway
R-HSA-2122948 Activated NOTCH1 Transmits Signal to the Nucleus
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ96J02
SIGNORiQ96J02

Miscellaneous databases

ChiTaRSiITCH human
EvolutionaryTraceiQ96J02
GenomeRNAii83737
PROiPR:Q96J02
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000078747 Expressed in 217 organ(s), highest expression level in sperm
CleanExiHS_ITCH
ExpressionAtlasiQ96J02 baseline and differential
GenevisibleiQ96J02 HS

Family and domain databases

CDDicd00078 HECTc, 1 hit
cd00201 WW, 4 hits
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR024928 E3_ub_ligase_SMURF1
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00632 HECT, 1 hit
PF00397 WW, 4 hits
PIRSFiPIRSF001569 E3_ub_ligase_SMURF1, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00119 HECTc, 1 hit
SM00456 WW, 4 hits
SUPFAMiSSF51045 SSF51045, 4 hits
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50237 HECT, 1 hit
PS01159 WW_DOMAIN_1, 4 hits
PS50020 WW_DOMAIN_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiITCH_HUMAN
AccessioniPrimary (citable) accession number: Q96J02
Secondary accession number(s): A6NEW4
, B4E234, E1P5P3, F5H217, O43584, Q5QP37, Q5TEL0, Q96F66, Q9BY75, Q9H451, Q9H4U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: November 7, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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