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Protein

THO complex subunit 3

Gene

THOC3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for efficient export of polyadenylated RNA and spliced mRNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.5 Publications

Caution

There are two almost identical copies of this gene on chromosome 5q35. One copy is frameshifted and unlikely to encode a functional protein.Curated

GO - Molecular functioni

GO - Biological processi

  • mRNA 3'-end processing Source: Reactome
  • mRNA export from nucleus Source: UniProtKB
  • RNA export from nucleus Source: Reactome
  • RNA splicing Source: UniProtKB-KW
  • viral mRNA export from host cell nucleus Source: UniProtKB

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-72187 mRNA 3'-end processing
SignaLinkiQ96J01

Names & Taxonomyi

Protein namesi
Recommended name:
THO complex subunit 3
Short name:
Tho3
Alternative name(s):
TEX1 homolog
hTREX45
Gene namesi
Name:THOC3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000051596.9
HGNCiHGNC:19072 THOC3
MIMi606929 gene
neXtProtiNX_Q96J01

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000051596
PharmGKBiPA134893535

Polymorphism and mutation databases

DMDMi48474597

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000512732 – 351THO complex subunit 3Add BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ96J01
MaxQBiQ96J01
PaxDbiQ96J01
PeptideAtlasiQ96J01
PRIDEiQ96J01
ProteomicsDBi76881

PTM databases

iPTMnetiQ96J01
PhosphoSitePlusiQ96J01

Expressioni

Gene expression databases

BgeeiENSG00000051596
CleanExiHS_THOC3
ExpressionAtlasiQ96J01 baseline and differential
GenevisibleiQ96J01 HS

Organism-specific databases

HPAiHPA042764
HPA044009
HPA045071

Interactioni

Subunit structurei

Component of the THO complex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7; together with at least ALYREF/THOC4, DDX39B, SARNP/CIP29 and CHTOP, THO forms the transcription/export (TREX) complex which seems to have a dynamic structure involving ATP-dependent remodeling.3 Publications

Protein-protein interaction databases

BioGridi124047, 34 interactors
CORUMiQ96J01
IntActiQ96J01, 13 interactors
MINTiQ96J01
STRINGi9606.ENSP00000265097

Structurei

3D structure databases

ProteinModelPortaliQ96J01
SMRiQ96J01
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati53 – 94WD 1Add BLAST42
Repeati97 – 137WD 2Add BLAST41
Repeati139 – 178WD 3Add BLAST40
Repeati180 – 221WD 4Add BLAST42
Repeati222 – 261WD 5Add BLAST40
Repeati264 – 303WD 6Add BLAST40

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1407 Eukaryota
ENOG410XPZJ LUCA
GeneTreeiENSGT00890000139430
HOGENOMiHOG000264781
HOVERGENiHBG059732
InParanoidiQ96J01
KOiK12880
OMAiLWDTTDW
OrthoDBiEOG091G0BFM
PhylomeDBiQ96J01
TreeFamiTF314069

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR024977 Apc4_WD40_dom
IPR020472 G-protein_beta_WD-40_rep
IPR013979 TIF_beta_prop-like
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12894 ANAPC4_WD40, 1 hit
PF08662 eIF2A, 1 hit
PF00400 WD40, 2 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96J01-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVPAAAMGP SALGQSGPGS MAPWCSVSSG PSRYVLGMQE LFRGHSKTRE
60 70 80 90 100
FLAHSAKVHS VAWSCDGRRL ASGSFDKTAS VFLLEKDRLV KENNYRGHGD
110 120 130 140 150
SVDQLCWHPS NPDLFVTASG DKTIRIWDVR TTKCIATVNT KGENINICWS
160 170 180 190 200
PDGQTIAVGN KDDVVTFIDA KTHRSKAEEQ FKFEVNEISW NNDNNMFFLT
210 220 230 240 250
NGNGCINILS YPELKPVQSI NAHPSNCICI KFDPMGKYFA TGSADALVSL
260 270 280 290 300
WDVDELVCVR CFSRLDWPVR TLSFSHDGKM LASASEDHFI DIAEVETGDK
310 320 330 340 350
LWEVQCESPT FTVAWHPKRP LLAFACDDKD GKYDSSREAG TVKLFGLPND

S
Length:351
Mass (Da):38,772
Last modified:December 1, 2001 - v1
Checksum:i9BA03D9D5571E424
GO
Isoform 2 (identifier: Q96J01-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     299-351: DKLWEVQCES...VKLFGLPNDS → NFMRIYRLSPLAVRTSLVISSLHVTTSPA

Note: No experimental confirmation available.
Show »
Length:327
Mass (Da):36,032
Checksum:iA0975A5DE8E4E830
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056173299 – 351DKLWE…LPNDS → NFMRIYRLSPLAVRTSLVIS SLHVTTSPA in isoform 2. 1 PublicationAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC138965 Genomic DNA No translation available.
AC139491 Genomic DNA No translation available.
BC006849 mRNA Translation: AAH06849.1
BC066325 mRNA Translation: AAH66325.1
BC068499 mRNA Translation: AAH68499.1
CCDSiCCDS4397.1 [Q96J01-1]
RefSeqiNP_115737.1, NM_032361.3 [Q96J01-1]
UniGeneiHs.484227
Hs.535769
Hs.715943

Genome annotation databases

EnsembliENST00000265097; ENSP00000265097; ENSG00000051596 [Q96J01-1]
ENST00000513482; ENSP00000422243; ENSG00000051596 [Q96J01-2]
GeneIDi84321
KEGGihsa:84321
UCSCiuc003mdg.6 human [Q96J01-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTHOC3_HUMAN
AccessioniPrimary (citable) accession number: Q96J01
Secondary accession number(s): Q6NZ53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: December 1, 2001
Last modified: June 20, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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