UniProtKB - Q96IZ6 (MET2A_HUMAN)
Protein
tRNA N(3)-methylcytidine methyltransferase METTL2A
Gene
METTL2A
Organism
Homo sapiens (Human)
Status
Functioni
S-adenosyl-L-methionine-dependent methyltransferase that mediates N3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Thr)(UGU) and tRNA(Arg)(CCU).1 Publication
Catalytic activityi
- cytidine32 in tRNAThr + S-adenosyl-L-methionine = H+ + N3-methylcytidine32 in tRNAThr + S-adenosyl-L-homocysteine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- cytidine32 in tRNAArg(CCU) + S-adenosyl-L-methionine = H+ + N3-methylcytidine32 in tRNAArg(CCU) + S-adenosyl-L-homocysteine1 PublicationThis reaction proceeds in the forward1 Publication direction.
GO - Molecular functioni
- tRNA (cytosine) methyltransferase activity Source: UniProtKB
- tRNA (cytosine-3-)-methyltransferase activity Source: UniProtKB
GO - Biological processi
- tRNA methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
PathwayCommonsi | Q96IZ6 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA N(3)-methylcytidine methyltransferase METTL2ACurated (EC:2.1.1.-1 Publication)Alternative name(s): Methyltransferase-like protein 2ACurated |
Gene namesi | ORF Names:HSPC266 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25755, METTL2A |
MIMi | 618902, gene |
neXtProti | NX_Q96IZ6 |
VEuPathDBi | HostDB:ENSG00000087995.15 |
Pathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000087995 |
PharmGKBi | PA143485532 |
Miscellaneous databases
Pharosi | Q96IZ6, Tdark |
Genetic variation databases
BioMutai | METTL2A |
DMDMi | 269849766 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000204452 | 2 – 378 | tRNA N(3)-methylcytidine methyltransferase METTL2AAdd BLAST | 377 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 4 | PhosphoserineBy similarity | 1 | |
Modified residuei | 154 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q96IZ6 |
jPOSTi | Q96IZ6 |
MassIVEi | Q96IZ6 |
MaxQBi | Q96IZ6 |
PaxDbi | Q96IZ6 |
PeptideAtlasi | Q96IZ6 |
PRIDEi | Q96IZ6 |
ProteomicsDBi | 76877 [Q96IZ6-1] 76878 [Q96IZ6-2] |
PTM databases
iPTMneti | Q96IZ6 |
PhosphoSitePlusi | Q96IZ6 |
Expressioni
Gene expression databases
Bgeei | ENSG00000087995, Expressed in heart and 116 other tissues |
ExpressionAtlasi | Q96IZ6, baseline and differential |
Genevisiblei | Q96IZ6, HS |
Organism-specific databases
HPAi | ENSG00000087995, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 130836, 17 interactors |
IntActi | Q96IZ6, 15 interactors |
MINTi | Q96IZ6 |
STRINGi | 9606.ENSP00000309610 |
Miscellaneous databases
RNActi | Q96IZ6, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2361, Eukaryota |
GeneTreei | ENSGT00940000156059 |
HOGENOMi | CLU_029724_0_1_1 |
InParanoidi | Q96IZ6 |
OMAi | RCWLQGR |
PhylomeDBi | Q96IZ6 |
TreeFami | TF323232 |
Family and domain databases
InterProi | View protein in InterPro IPR026113, MeTrfase IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR22809, PTHR22809, 1 hit |
PIRSFi | PIRSF037755, Mettl2_prd, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96IZ6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGSYPEGAP AVLADKRQQF GSRFLRDPAR VFHHNAWDNV EWSEEQAAAA
60 70 80 90 100
ERKVQENSIQ RVCQEKQVDY EINAHKYWND FYKIHENGFF KDRHWLFTEF
110 120 130 140 150
PELAPSQNQN HLKDWFLENK SEVPECRNNE DGPGLIMEEQ HKCSSKSLEH
160 170 180 190 200
KTQTLPVEEN VTQKISDLEI CADEFPGSSA TYRILEVGCG VGNTVFPILQ
210 220 230 240 250
TNNDPGLFVY CCDFSSTAIE LVQTNSEYDP SRCFAFVHDL CDEEKSYPVP
260 270 280 290 300
KGSLDIIILI FVLSAIVPDK MQKAINRLSR LLKPGGMMLL RDYGRYDMAQ
310 320 330 340 350
LRFKKGQCLS GNFYVRGDGT RVYFFTQEEL DTLFTTAGLE KVQNLVDRRL
360 370
QVNRGKQLTM YRVWIQCKYC KPLLSSTS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WW35 | A0A087WW35_HUMAN | Methyltransferase-like protein | METTL2A | 313 | Annotation score: |
Sequence cautioni
The sequence BAB14260 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 118 | E → K in AAF28944 (PubMed:11042152).Curated | 1 | |
Sequence conflicti | 142 | K → R in BAB14260 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 155 | L → P in AAH06985 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 155 | L → P in AAF28944 (PubMed:11042152).Curated | 1 | |
Sequence conflicti | 155 | L → P in BAB14260 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 262 | V → G in AAF28944 (PubMed:11042152).Curated | 1 | |
Sequence conflicti | 273 | K → R in AAF28944 (PubMed:11042152).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008477 | 1 – 136 | Missing in isoform 2. 1 PublicationAdd BLAST | 136 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC008026 Genomic DNA No translation available. BC006985 mRNA Translation: AAH06985.1 AF161384 mRNA Translation: AAF28944.1 AK022822 mRNA Translation: BAB14260.1 Different initiation. |
CCDSi | CCDS45752.1 [Q96IZ6-1] |
RefSeqi | NP_859076.3, NM_181725.3 [Q96IZ6-1] |
Genome annotation databases
Ensembli | ENST00000311506; ENSP00000309610; ENSG00000087995 [Q96IZ6-1] |
GeneIDi | 339175 |
KEGGi | hsa:339175 |
UCSCi | uc002izv.3, human [Q96IZ6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC008026 Genomic DNA No translation available. BC006985 mRNA Translation: AAH06985.1 AF161384 mRNA Translation: AAF28944.1 AK022822 mRNA Translation: BAB14260.1 Different initiation. |
CCDSi | CCDS45752.1 [Q96IZ6-1] |
RefSeqi | NP_859076.3, NM_181725.3 [Q96IZ6-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 130836, 17 interactors |
IntActi | Q96IZ6, 15 interactors |
MINTi | Q96IZ6 |
STRINGi | 9606.ENSP00000309610 |
PTM databases
iPTMneti | Q96IZ6 |
PhosphoSitePlusi | Q96IZ6 |
Genetic variation databases
BioMutai | METTL2A |
DMDMi | 269849766 |
Proteomic databases
EPDi | Q96IZ6 |
jPOSTi | Q96IZ6 |
MassIVEi | Q96IZ6 |
MaxQBi | Q96IZ6 |
PaxDbi | Q96IZ6 |
PeptideAtlasi | Q96IZ6 |
PRIDEi | Q96IZ6 |
ProteomicsDBi | 76877 [Q96IZ6-1] 76878 [Q96IZ6-2] |
Protocols and materials databases
Antibodypediai | 57006, 118 antibodies |
DNASUi | 339175 |
Genome annotation databases
Ensembli | ENST00000311506; ENSP00000309610; ENSG00000087995 [Q96IZ6-1] |
GeneIDi | 339175 |
KEGGi | hsa:339175 |
UCSCi | uc002izv.3, human [Q96IZ6-1] |
Organism-specific databases
CTDi | 339175 |
GeneCardsi | METTL2A |
HGNCi | HGNC:25755, METTL2A |
HPAi | ENSG00000087995, Low tissue specificity |
MIMi | 618902, gene |
neXtProti | NX_Q96IZ6 |
OpenTargetsi | ENSG00000087995 |
PharmGKBi | PA143485532 |
VEuPathDBi | HostDB:ENSG00000087995.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2361, Eukaryota |
GeneTreei | ENSGT00940000156059 |
HOGENOMi | CLU_029724_0_1_1 |
InParanoidi | Q96IZ6 |
OMAi | RCWLQGR |
PhylomeDBi | Q96IZ6 |
TreeFami | TF323232 |
Enzyme and pathway databases
PathwayCommonsi | Q96IZ6 |
Miscellaneous databases
BioGRID-ORCSi | 339175, 4 hits in 539 CRISPR screens |
ChiTaRSi | METTL2A, human |
GenomeRNAii | 339175 |
Pharosi | Q96IZ6, Tdark |
PROi | PR:Q96IZ6 |
RNActi | Q96IZ6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000087995, Expressed in heart and 116 other tissues |
ExpressionAtlasi | Q96IZ6, baseline and differential |
Genevisiblei | Q96IZ6, HS |
Family and domain databases
InterProi | View protein in InterPro IPR026113, MeTrfase IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR22809, PTHR22809, 1 hit |
PIRSFi | PIRSF037755, Mettl2_prd, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MET2A_HUMAN | |
Accessioni | Q96IZ6Primary (citable) accession number: Q96IZ6 Secondary accession number(s): A6NNC4 Q9P0B5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2003 |
Last sequence update: | November 24, 2009 | |
Last modified: | February 10, 2021 | |
This is version 165 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families