UniProtKB - Q96IZ0 (PAWR_HUMAN)
PRKC apoptosis WT1 regulator protein
PAWR
Functioni
Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
1 PublicationGO - Molecular functioni
- actin binding Source: UniProtKB
- enzyme binding Source: UniProtKB
- leucine zipper domain binding Source: UniProtKB
- transcription corepressor activity Source: ProtInc
GO - Biological processi
- actin filament bundle assembly Source: UniProtKB
- apoptotic process Source: UniProtKB
- apoptotic signaling pathway Source: Ensembl
- negative regulation of B cell proliferation Source: Ensembl
- negative regulation of fibroblast proliferation Source: Ensembl
- negative regulation of gene expression Source: Ensembl
- negative regulation of T cell proliferation Source: Ensembl
- negative regulation of T cell receptor signaling pathway Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: ProtInc
- positive regulation of amyloid precursor protein biosynthetic process Source: Ensembl
- positive regulation of apoptotic process Source: GO_Central
- positive regulation of cellular senescence Source: Ensembl
- positive regulation of gene expression Source: Ensembl
- positive regulation of hydrogen peroxide-mediated programmed cell death Source: Ensembl
Keywordsi
Biological process | Apoptosis, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q96IZ0 |
SignaLinki | Q96IZ0 |
SIGNORi | Q96IZ0 |
Names & Taxonomyi
Protein namesi | Recommended name: PRKC apoptosis WT1 regulator proteinAlternative name(s): Prostate apoptosis response 4 protein Short name: Par-4 |
Gene namesi | Name:PAWR Synonyms:PAR4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8614, PAWR |
MIMi | 601936, gene |
neXtProti | NX_Q96IZ0 |
VEuPathDBi | HostDB:ENSG00000177425 |
Subcellular locationi
Cytoplasm and Cytosol
Nucleus
Note: Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies.By similarity
Cytoskeleton
- actin cytoskeleton Source: HPA
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: HPA
Other locations
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 66 | A → D: No loss of interaction with SPSB1, SPSB2 and SPSB4. 1 Publication | 1 | |
Mutagenesisi | 68 | E → D: Increased interaction with SPSB2. Only slightly increased interaction with SPSB4. Increased interaction with SPSB1, SPSB2 and SPSB4; when associated with A-69. 1 Publication | 1 | |
Mutagenesisi | 69 | L → I: Only slighlty increased interaction with SPSB2. Only slightly increased interaction with SPSB4. Increased interaction with SPSB1, SPSB2 and SPSB4; when associated with A-68. 1 Publication | 1 | |
Mutagenesisi | 71 | N → A: Loss of interaction with SPSB1, SPSB2 and SPSB4. 1 Publication | 1 | |
Mutagenesisi | 72 | N → A: Loss of interaction with SPSB1-Elongin BC complex and SPSB2 and SPSB4. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 5074 |
OpenTargetsi | ENSG00000177425 |
PharmGKBi | PA32954 |
Miscellaneous databases
Pharosi | Q96IZ0, Tbio |
Genetic variation databases
BioMutai | PAWR |
DMDMi | 66773935 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058236 | 1 – 340 | PRKC apoptosis WT1 regulator proteinAdd BLAST | 340 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 108 | PhosphoserineCombined sources | 1 | |
Modified residuei | 163 | Phosphothreonine; by PKABy similarity | 1 | |
Modified residuei | 231 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q96IZ0 |
jPOSTi | Q96IZ0 |
MassIVEi | Q96IZ0 |
MaxQBi | Q96IZ0 |
PaxDbi | Q96IZ0 |
PeptideAtlasi | Q96IZ0 |
PRIDEi | Q96IZ0 |
ProteomicsDBi | 76870 |
TopDownProteomicsi | Q96IZ0 |
PTM databases
GlyGeni | Q96IZ0, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96IZ0 |
PhosphoSitePlusi | Q96IZ0 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000177425, Expressed in testis and 225 other tissues |
ExpressionAtlasi | Q96IZ0, baseline and differential |
Genevisiblei | Q96IZ0, HS |
Organism-specific databases
HPAi | ENSG00000177425, Low tissue specificity |
Interactioni
Subunit structurei
Homooligomer.
Interacts (via the C-terminal region) with WT1 (PubMed:8943350).
Interacts with THAP1 (PubMed:12717420).
Interacts with AATF (PubMed:14627703).
Interacts with BACE1 (PubMed:15671026).
Interacts with SPSB1 (via B30.2/SPRY domain); this interaction is direct and occurs in association with the Elongin BC complex (PubMed:17189197, PubMed:20561531).
Interacts with SPSB2 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex (PubMed:17189197, PubMed:20561531).
Interacts with SPSB4 (via B30.2/SPRY domain) (PubMed:20561531); this interaction occurs in association with the Elongin BC complex (PubMed:20561531).
Component of a ternary complex composed of SQSTM1 and PRKCZ (PubMed:11755531).
Interacts with actin (By similarity).
By similarity7 PublicationsBinary interactionsi
Q96IZ0
GO - Molecular functioni
- actin binding Source: UniProtKB
- enzyme binding Source: UniProtKB
- leucine zipper domain binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 111108, 76 interactors |
CORUMi | Q96IZ0 |
DIPi | DIP-29003N |
ELMi | Q96IZ0 |
IntActi | Q96IZ0, 26 interactors |
MINTi | Q96IZ0 |
STRINGi | 9606.ENSP00000328088 |
Chemistry databases
BindingDBi | Q96IZ0 |
Miscellaneous databases
RNActi | Q96IZ0, protein |
Structurei
3D structure databases
SMRi | Q96IZ0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96IZ0 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 253 | DisorderedSequence analysisAdd BLAST | 253 | |
Regioni | 145 – 203 | Selective for apoptosis induction in cancer cells (SAC)Add BLAST | 59 | |
Regioni | 300 – 340 | Leucine-zipperAdd BLAST | 41 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 186 – 206 | Sequence analysisAdd BLAST | 21 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 68 – 72 | B30.2/SPRY domain-binding motif1 Publication | 5 | |
Motifi | 145 – 161 | Nuclear localization signalBy similarityAdd BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 16 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 147 – 162 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 216 – 237 | Polar residuesSequence analysisAdd BLAST | 22 |
Domaini
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QVUF, Eukaryota |
GeneTreei | ENSGT00390000000406 |
HOGENOMi | CLU_076619_0_0_1 |
InParanoidi | Q96IZ0 |
OMAi | TPMACND |
OrthoDBi | 1363165at2759 |
PhylomeDBi | Q96IZ0 |
TreeFami | TF332824 |
Family and domain databases
DisProti | DP01603 |
IDEALi | IID00177 |
InterProi | View protein in InterPro IPR026117, Par-4 |
PANTHERi | PTHR15093, PTHR15093, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MATGGYRTSS GLGGSTTDFL EEWKAKREKM RAKQNPPGPA PPGGGSSDAA
60 70 80 90 100
GKPPAGALGT PAAAAANELN NNLPGGAPAA PAVPGPGGVN CAVGSAMLTR
110 120 130 140 150
AAPGPRRSED EPPAASASAA PPPQRDEEEP DGVPEKGKSS GPSARKGKGQ
160 170 180 190 200
IEKRKLREKR RSTGVVNIPA AECLDEYEDD EAGQKERKRE DAITQQNTIQ
210 220 230 240 250
NEAVNLLDPG SSYLLQEPPR TVSGRYKSTT SVSEEDVSSR YSRTDRSGFP
260 270 280 290 300
RYNRDANVSG TLVSSSTLEK KIEDLEKEVV RERQENLRLV RLMQDKEEMI
310 320 330 340
GKLKEEIDLL NRDLDDIEDE NEQLKQENKT LLKVVGQLTR
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8W1M8 | F8W1M8_HUMAN | PRKC apoptosis WT1 regulator protei... | PAWR | 75 | Annotation score: | ||
H0YI16 | H0YI16_HUMAN | PRKC apoptosis WT1 regulator protei... | PAWR | 194 | Annotation score: | ||
A0A0B4J260 | A0A0B4J260_HUMAN | PRKC apoptosis WT1 regulator protei... | PAWR | 63 | Annotation score: | ||
H0YHP5 | H0YHP5_HUMAN | PRKC apoptosis WT1 regulator protei... | PAWR | 72 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 102 – 103 | AP → PPAR in AAC24947 (PubMed:8943350).Curated | 2 | |
Sequence conflicti | 199 | I → M in CAG38786 (Ref. 2) Curated | 1 | |
Sequence conflicti | 281 | R → T in AAC24947 (PubMed:8943350).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_022465 | 42 | P → L1 PublicationCorresponds to variant dbSNP:rs8176804Ensembl. | 1 | |
Natural variantiVAR_022466 | 78 | P → R2 PublicationsCorresponds to variant dbSNP:rs8176805Ensembl. | 1 | |
Natural variantiVAR_022467 | 137 | G → A1 PublicationCorresponds to variant dbSNP:rs8176806Ensembl. | 1 | |
Natural variantiVAR_022468 | 202 | E → A1 PublicationCorresponds to variant dbSNP:rs8176870Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U63809 mRNA Translation: AAC24947.1 CR536549 mRNA Translation: CAG38786.1 AY300794 Genomic DNA Translation: AAP43693.1 BC007018 mRNA Translation: AAH07018.1 AF503628 Genomic DNA Translation: AAM27453.1 |
CCDSi | CCDS31863.1 |
RefSeqi | NP_002574.2, NM_002583.2 XP_006719498.1, XM_006719435.2 XP_016874866.1, XM_017019377.1 |
Genome annotation databases
Ensembli | ENST00000328827; ENSP00000328088; ENSG00000177425 |
GeneIDi | 5074 |
KEGGi | hsa:5074 |
MANE-Selecti | ENST00000328827.9; ENSP00000328088.4; NM_002583.4; NP_002574.2 |
UCSCi | uc001syx.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U63809 mRNA Translation: AAC24947.1 CR536549 mRNA Translation: CAG38786.1 AY300794 Genomic DNA Translation: AAP43693.1 BC007018 mRNA Translation: AAH07018.1 AF503628 Genomic DNA Translation: AAM27453.1 |
CCDSi | CCDS31863.1 |
RefSeqi | NP_002574.2, NM_002583.2 XP_006719498.1, XM_006719435.2 XP_016874866.1, XM_017019377.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JK9 | X-ray | 1.79 | B | 67-81 | [»] | |
SMRi | Q96IZ0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111108, 76 interactors |
CORUMi | Q96IZ0 |
DIPi | DIP-29003N |
ELMi | Q96IZ0 |
IntActi | Q96IZ0, 26 interactors |
MINTi | Q96IZ0 |
STRINGi | 9606.ENSP00000328088 |
Chemistry databases
BindingDBi | Q96IZ0 |
PTM databases
GlyGeni | Q96IZ0, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96IZ0 |
PhosphoSitePlusi | Q96IZ0 |
Genetic variation databases
BioMutai | PAWR |
DMDMi | 66773935 |
Proteomic databases
EPDi | Q96IZ0 |
jPOSTi | Q96IZ0 |
MassIVEi | Q96IZ0 |
MaxQBi | Q96IZ0 |
PaxDbi | Q96IZ0 |
PeptideAtlasi | Q96IZ0 |
PRIDEi | Q96IZ0 |
ProteomicsDBi | 76870 |
TopDownProteomicsi | Q96IZ0 |
Protocols and materials databases
Antibodypediai | 1824, 388 antibodies from 40 providers |
DNASUi | 5074 |
Genome annotation databases
Ensembli | ENST00000328827; ENSP00000328088; ENSG00000177425 |
GeneIDi | 5074 |
KEGGi | hsa:5074 |
MANE-Selecti | ENST00000328827.9; ENSP00000328088.4; NM_002583.4; NP_002574.2 |
UCSCi | uc001syx.4, human |
Organism-specific databases
CTDi | 5074 |
DisGeNETi | 5074 |
GeneCardsi | PAWR |
HGNCi | HGNC:8614, PAWR |
HPAi | ENSG00000177425, Low tissue specificity |
MIMi | 601936, gene |
neXtProti | NX_Q96IZ0 |
OpenTargetsi | ENSG00000177425 |
PharmGKBi | PA32954 |
VEuPathDBi | HostDB:ENSG00000177425 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QVUF, Eukaryota |
GeneTreei | ENSGT00390000000406 |
HOGENOMi | CLU_076619_0_0_1 |
InParanoidi | Q96IZ0 |
OMAi | TPMACND |
OrthoDBi | 1363165at2759 |
PhylomeDBi | Q96IZ0 |
TreeFami | TF332824 |
Enzyme and pathway databases
PathwayCommonsi | Q96IZ0 |
SignaLinki | Q96IZ0 |
SIGNORi | Q96IZ0 |
Miscellaneous databases
BioGRID-ORCSi | 5074, 22 hits in 1048 CRISPR screens |
ChiTaRSi | PAWR, human |
EvolutionaryTracei | Q96IZ0 |
GeneWikii | PAWR |
GenomeRNAii | 5074 |
Pharosi | Q96IZ0, Tbio |
PROi | PR:Q96IZ0 |
RNActi | Q96IZ0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000177425, Expressed in testis and 225 other tissues |
ExpressionAtlasi | Q96IZ0, baseline and differential |
Genevisiblei | Q96IZ0, HS |
Family and domain databases
DisProti | DP01603 |
IDEALi | IID00177 |
InterProi | View protein in InterPro IPR026117, Par-4 |
PANTHERi | PTHR15093, PTHR15093, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PAWR_HUMAN | |
Accessioni | Q96IZ0Primary (citable) accession number: Q96IZ0 Secondary accession number(s): O75796, Q6FHY9, Q8N700 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2005 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references