Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial

Gene

SUCLG2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.UniRule annotation

Catalytic activityi

GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (SUCLG2), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (SUCLG1), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (SUCLG1), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (sucD), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (SUCLG1), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2), Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (SUCLG2), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (sucC), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (SUCLG1), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57GTPUniRule annotation1
Sitei79Important for substrate specificityUniRule annotation1
Binding sitei146GTP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Sitei147Important for substrate specificityUniRule annotation1
Metal bindingi243MagnesiumUniRule annotation1
Metal bindingi257MagnesiumUniRule annotation1
Binding sitei308Substrate; shared with subunit alphaUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi90 – 92GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processTricarboxylic acid cycle
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS10493-MONOMER
BRENDAi6.2.1.4 2681
ReactomeiR-HSA-71403 Citric acid cycle (TCA cycle)
UniPathwayi
UPA00223;UER00999

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrialUniRule annotation (EC:6.2.1.4UniRule annotation)
Alternative name(s):
GTP-specific succinyl-CoA synthetase subunit betaUniRule annotation
Short name:
G-SCSUniRule annotation
Short name:
GTPSCSUniRule annotation
Succinyl-CoA synthetase beta-G chainUniRule annotation
Short name:
SCS-betaGUniRule annotation
Gene namesi
Name:SUCLG2UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000172340.14
HGNCiHGNC:11450 SUCLG2
MIMi603922 gene
neXtProtiNX_Q96I99

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi8801
OpenTargetsiENSG00000172340
PharmGKBiPA36247

Chemistry databases

DrugBankiDB00139 Succinic acid

Polymorphism and mutation databases

BioMutaiSUCLG2
DMDMi52788292

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37MitochondrionCombined sourcesAdd BLAST37
ChainiPRO_000003335638 – 432Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrialAdd BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73N6-acetyllysineBy similarity1
Modified residuei78N6-succinyllysineBy similarity1
Modified residuei132N6-acetyllysineBy similarity1
Modified residuei139N6-acetyllysineBy similarity1
Modified residuei161PhosphoserineBy similarity1
Modified residuei200N6-acetyllysineBy similarity1
Modified residuei218N6-acetyllysineBy similarity1
Modified residuei227N6-acetyllysineCombined sources1
Modified residuei271N6-acetyllysineBy similarity1
Modified residuei291N6-acetyllysineCombined sources1
Modified residuei338N6-succinyllysineBy similarity1
Modified residuei347N6-acetyllysineBy similarity1
Modified residuei386N6-acetyllysineBy similarity1
Modified residuei423N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96I99
MaxQBiQ96I99
PaxDbiQ96I99
PeptideAtlasiQ96I99
PRIDEiQ96I99
ProteomicsDBi76820
76821 [Q96I99-2]
TopDownProteomicsiQ96I99-1 [Q96I99-1]
Q96I99-2 [Q96I99-2]

2D gel databases

REPRODUCTION-2DPAGEiIPI00096066

PTM databases

iPTMnetiQ96I99
PhosphoSitePlusiQ96I99
SwissPalmiQ96I99

Expressioni

Tissue specificityi

Mainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary.1 Publication

Gene expression databases

BgeeiENSG00000172340 Expressed in 222 organ(s), highest expression level in colonic mucosa
CleanExiHS_SUCLG2
ExpressionAtlasiQ96I99 baseline and differential
GenevisibleiQ96I99 HS

Organism-specific databases

HPAiHPA046705
HPA051998

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
HSCBQ8IWL34EBI-2511878,EBI-1805738

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114329, 42 interactors
CORUMiQ96I99
DIPiDIP-53563N
IntActiQ96I99, 11 interactors
MINTiQ96I99
STRINGi9606.ENSP00000419325

Structurei

3D structure databases

ProteinModelPortaliQ96I99
SMRiQ96I99
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 274ATP-graspUniRule annotationAdd BLAST229

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni365 – 367Substrate binding; shared with subunit alphaUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2799 Eukaryota
COG0045 LUCA
GeneTreeiENSGT00390000010170
HOGENOMiHOG000007059
HOVERGENiHBG055555
InParanoidiQ96I99
KOiK01900
OMAiVQIEINP
OrthoDBiEOG091G07T9
PhylomeDBiQ96I99
TreeFamiTF300624

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.40.50.261, 1 hit
HAMAPiMF_00558 Succ_CoA_beta, 1 hit
MF_03221 Succ_CoA_betaG_euk, 1 hit
InterProiView protein in InterPro
IPR013650 ATP-grasp_succ-CoA_synth-type
IPR013815 ATP_grasp_subdomain_1
IPR005811 CoA_ligase
IPR017866 Succ-CoA_synthase_bsu_CS
IPR034722 Succ_CoA_betaG_euk
IPR005809 Succ_CoA_synthase_bsu
IPR016102 Succinyl-CoA_synth-like
PANTHERiPTHR11815 PTHR11815, 1 hit
PfamiView protein in Pfam
PF08442 ATP-grasp_2, 1 hit
PF00549 Ligase_CoA, 1 hit
PIRSFiPIRSF001554 SucCS_beta, 1 hit
SUPFAMiSSF52210 SSF52210, 1 hit
TIGRFAMsiTIGR01016 sucCoAbeta, 1 hit
PROSITEiView protein in PROSITE
PS01217 SUCCINYL_COA_LIG_3, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q96I99-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASPVAAQAG KLLRALALRP RFLAAGSQAV QLTSRRWLNL QEYQSKKLMS
60 70 80 90 100
DNGVRVQRFF VADTANEALE AAKRLNAKEI VLKAQILAGG RGKGVFNSGL
110 120 130 140 150
KGGVHLTKDP NVVGQLAKQM IGYNLATKQT PKEGVKVNKV MVAEALDISR
160 170 180 190 200
ETYLAILMDR SCNGPVLVGS PQGGVDIEEV AASNPELIFK EQIDIFEGIK
210 220 230 240 250
DSQAQRMAEN LGFVGPLKSQ AADQITKLYN LFLKIDATQV EVNPFGETPE
260 270 280 290 300
GQVVCFDAKI NFDDNAEFRQ KDIFAMDDKS ENEPIENEAA KYDLKYIGLD
310 320 330 340 350
GNIACFVNGA GLAMATCDII FLNGGKPANF LDLGGGVKEA QVYQAFKLLT
360 370 380 390 400
ADPKVEAILV NIFGGIVNCA IIANGITKAC RELELKVPLV VRLEGTNVQE
410 420 430
AQKILNNSGL PITSAIDLED AAKKAVASVA KK
Length:432
Mass (Da):46,511
Last modified:September 27, 2004 - v2
Checksum:i56A977A3E50713A1
GO
Isoform 2 (identifier: Q96I99-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     397-432: NVQEAQKILN...KKAVASVAKK → FMEKKGSYMH...SKCAISIFLC

Note: No experimental confirmation available.
Show »
Length:440
Mass (Da):47,732
Checksum:iFFBA486CDA19334B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y852H0Y852_HUMAN
Succinate--CoA ligase [GDP-forming]...
SUCLG2
190Annotation score:
E9PDQ8E9PDQ8_HUMAN
Succinate--CoA ligase [GDP-forming]...
SUCLG2
379Annotation score:

Sequence cautioni

The sequence AAH07716 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH47024 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH47024 differs from that shown. Reason: Erroneous termination at position 31. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174 – 175GV → RS in AAC64397 (PubMed:9765291).Curated2
Sequence conflicti220Q → K in AAH68602 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052499347K → R. Corresponds to variant dbSNP:rs9843840Ensembl.1
Natural variantiVAR_052500381R → W. Corresponds to variant dbSNP:rs7623258EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042013397 – 432NVQEA…SVAKK → FMEKKGSYMHIKQETGNSNE NITGIQENVAHQNLSKCAIS IFLC in isoform 2. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK310527 mRNA No translation available.
AC099783 Genomic DNA No translation available.
AC112213 Genomic DNA No translation available.
AC113169 Genomic DNA No translation available.
AC114401 Genomic DNA No translation available.
BC007716 mRNA Translation: AAH07716.3 Different initiation.
BC019868 mRNA Translation: AAH19868.1
BC047024 mRNA Translation: AAH47024.1 Sequence problems.
BC068602 mRNA Translation: AAH68602.1
AF058954 mRNA Translation: AAC64397.1
AF131748 mRNA Translation: AAD20032.1
CCDSiCCDS43104.1 [Q96I99-1]
CCDS54605.1 [Q96I99-2]
RefSeqiNP_001171070.1, NM_001177599.1 [Q96I99-2]
NP_003839.2, NM_003848.3 [Q96I99-1]
UniGeneiHs.644919

Genome annotation databases

EnsembliENST00000307227; ENSP00000307432; ENSG00000172340 [Q96I99-1]
ENST00000493112; ENSP00000419325; ENSG00000172340 [Q96I99-2]
GeneIDi8801
KEGGihsa:8801
UCSCiuc003dna.5 human [Q96I99-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSUCB2_HUMAN
AccessioniPrimary (citable) accession number: Q96I99
Secondary accession number(s): C9JVT2
, O95195, Q6NUH7, Q86VX8, Q8WUQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: September 27, 2004
Last modified: September 12, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again