UniProtKB - Q96I15 (SCLY_HUMAN)
Protein
Selenocysteine lyase
Gene
SCLY
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium.By similarity
Catalytic activityi
- EC:4.4.1.16
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 388 | S-selanylcysteine intermediateBy similarity | 1 |
GO - Molecular functioni
- selenocysteine lyase activity Source: ProtInc
- transferase activity Source: UniProtKB-KW
GO - Biological processi
- cellular amino acid metabolic process Source: ProtInc
- selenium compound metabolic process Source: Reactome
Keywordsi
Molecular function | Lyase, Transferase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
PathwayCommonsi | Q96I15 |
Reactomei | R-HSA-2408508, Metabolism of ingested SeMet, Sec, MeSec into H2Se |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:SCLY Synonyms:SCL |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000132330.16 |
HGNCi | HGNC:18161, SCLY |
MIMi | 611056, gene |
neXtProti | NX_Q96I15 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 51540 |
OpenTargetsi | ENSG00000132330 |
PharmGKBi | PA134979359 |
Miscellaneous databases
Pharosi | Q96I15, Tbio |
Chemistry databases
DrugBanki | DB00114, Pyridoxal phosphate DB11135, Selenium |
Polymorphism and mutation databases
BioMutai | SCLY |
DMDMi | 527504087 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000317012 | 1 – 445 | Selenocysteine lyaseAdd BLAST | 445 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 129 | PhosphoserineCombined sources | 1 | |
Modified residuei | 259 | N6-(pyridoxal phosphate)lysine | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q96I15 |
jPOSTi | Q96I15 |
MassIVEi | Q96I15 |
MaxQBi | Q96I15 |
PaxDbi | Q96I15 |
PeptideAtlasi | Q96I15 |
PRIDEi | Q96I15 |
ProteomicsDBi | 76802 [Q96I15-1] 76803 [Q96I15-2] |
PTM databases
iPTMneti | Q96I15 |
PhosphoSitePlusi | Q96I15 |
Expressioni
Inductioni
Up-regulated in acute glomerulonephritis. Regulated by JUN/AP-1.1 Publication
Gene expression databases
Bgeei | ENSG00000132330, Expressed in right lobe of liver and 130 other tissues |
ExpressionAtlasi | Q96I15, baseline and differential |
Genevisiblei | Q96I15, HS |
Organism-specific databases
HPAi | ENSG00000132330, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
By similarityBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 119599, 32 interactors |
IntActi | Q96I15, 10 interactors |
MINTi | Q96I15 |
STRINGi | 9606.ENSP00000254663 |
Miscellaneous databases
RNActi | Q96I15, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q96I15 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96I15 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | KOG1549, Eukaryota |
GeneTreei | ENSGT00940000157773 |
HOGENOMi | CLU_003433_10_0_1 |
InParanoidi | Q96I15 |
OrthoDBi | 697150at2759 |
PhylomeDBi | Q96I15 |
TreeFami | TF313550 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR016454, Cysteine_dSase IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00266, Aminotran_5, 1 hit |
PIRSFi | PIRSF005572, NifS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96I15-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEAAVAPGRD APAPAASQPS GCGKHNSPER KVYMDYNATT PLEPEVIQAM
60 70 80 90 100
TKAMWEAWGN PSSPYSAGRK AKDIINAARE SLAKMIGGKP QDIIFTSGGT
110 120 130 140 150
ESNNLVIHSV VKHFHANQTS KGHTGGHHSP VKGAKPHFIT SSVEHDSIRL
160 170 180 190 200
PLEHLVEEQV AAVTFVPVSK VSGQAEVDDI LAAVRPTTRL VTIMLANNET
210 220 230 240 250
GIVMPVPEIS QRIKALNQER VAAGLPPILV HTDAAQALGK QRVDVEDLGV
260 270 280 290 300
DFLTIVGHKF YGPRIGALYI RGLGEFTPLY PMLFGGGQER NFRPGTENTP
310 320 330 340 350
MIAGLGKAAE LVTQNCEAYE AHMRDVRDYL EERLEAEFGQ KRIHLNSQFP
360 370 380 390 400
GTQRLPNTCN FSIRGPRLQG HVVLAQCRVL MASVGAACHS DHGDQPSPVL
410 420 430 440
LSYGVPFDVA RNALRLSVGR STTRAEVDLV VQDLKQAVAQ LEDQA
Computationally mapped potential isoform sequencesi
There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MQU4 | A0A0A0MQU4_HUMAN | Selenocysteine lyase | SCLY | 453 | Annotation score: | ||
C9JNL5 | C9JNL5_HUMAN | Selenocysteine lyase | SCLY | 162 | Annotation score: | ||
H7C4A1 | H7C4A1_HUMAN | Selenocysteine lyase | SCLY | 194 | Annotation score: | ||
F8WBU3 | F8WBU3_HUMAN | Selenocysteine lyase | SCLY | 73 | Annotation score: | ||
F8WCL3 | F8WCL3_HUMAN | Selenocysteine lyase | SCLY | 71 | Annotation score: | ||
H7C3V9 | H7C3V9_HUMAN | Selenocysteine lyase | SCLY | 241 | Annotation score: | ||
H7C1N7 | H7C1N7_HUMAN | Selenocysteine lyase | SCLY | 122 | Annotation score: | ||
H7C277 | H7C277_HUMAN | Selenocysteine lyase | SCLY | 251 | Annotation score: | ||
H7C280 | H7C280_HUMAN | Selenocysteine lyase | SCLY | 151 | Annotation score: | ||
H7C2M1 | H7C2M1_HUMAN | Selenocysteine lyase | SCLY | 109 | Annotation score: | ||
There is more potential isoformShow all |
Sequence cautioni
The sequence BAA91659 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 | M → MPGSSGAGM in EAW71139 (Ref. 3) Curated | 1 | |
Sequence conflicti | 42 | L → V in AAF36816 (PubMed:10692412).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_038464 | 52 | K → E. Corresponds to variant dbSNP:rs7597367Ensembl. | 1 | |
Natural variantiVAR_038465 | 175 | A → T1 PublicationCorresponds to variant dbSNP:rs3210400Ensembl. | 1 | |
Natural variantiVAR_038466 | 276 | F → S. Corresponds to variant dbSNP:rs35637307EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_030854 | 308 – 445 | AAELV…LEDQA → VSPGELEEMS in isoform 2. 1 PublicationAdd BLAST | 138 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF175767 mRNA Translation: AAF36816.1 AC016757 Genomic DNA Translation: AAY24333.1 AC016776 Genomic DNA Translation: AAY24221.1 CH471063 Genomic DNA Translation: EAW71139.1 BC000586 mRNA Translation: AAH00586.2 BC007891 mRNA Translation: AAH07891.1 AK001377 mRNA Translation: BAA91659.1 Different initiation. |
CCDSi | CCDS2524.2 [Q96I15-1] |
RefSeqi | NP_057594.4, NM_016510.5 [Q96I15-1] |
Genome annotation databases
Ensembli | ENST00000254663; ENSP00000254663; ENSG00000132330 [Q96I15-1] ENST00000409736; ENSP00000387162; ENSG00000132330 [Q96I15-2] |
GeneIDi | 51540 |
KEGGi | hsa:51540 |
UCSCi | uc061uct.1, human [Q96I15-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF175767 mRNA Translation: AAF36816.1 AC016757 Genomic DNA Translation: AAY24333.1 AC016776 Genomic DNA Translation: AAY24221.1 CH471063 Genomic DNA Translation: EAW71139.1 BC000586 mRNA Translation: AAH00586.2 BC007891 mRNA Translation: AAH07891.1 AK001377 mRNA Translation: BAA91659.1 Different initiation. |
CCDSi | CCDS2524.2 [Q96I15-1] |
RefSeqi | NP_057594.4, NM_016510.5 [Q96I15-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3GZC | X-ray | 2.10 | A/B | 8-445 | [»] | |
3GZD | X-ray | 1.80 | A/B/C/D | 8-445 | [»] | |
SMRi | Q96I15 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119599, 32 interactors |
IntActi | Q96I15, 10 interactors |
MINTi | Q96I15 |
STRINGi | 9606.ENSP00000254663 |
Chemistry databases
DrugBanki | DB00114, Pyridoxal phosphate DB11135, Selenium |
PTM databases
iPTMneti | Q96I15 |
PhosphoSitePlusi | Q96I15 |
Polymorphism and mutation databases
BioMutai | SCLY |
DMDMi | 527504087 |
Proteomic databases
EPDi | Q96I15 |
jPOSTi | Q96I15 |
MassIVEi | Q96I15 |
MaxQBi | Q96I15 |
PaxDbi | Q96I15 |
PeptideAtlasi | Q96I15 |
PRIDEi | Q96I15 |
ProteomicsDBi | 76802 [Q96I15-1] 76803 [Q96I15-2] |
Protocols and materials databases
Antibodypediai | 34490, 122 antibodies |
DNASUi | 51540 |
Genome annotation databases
Ensembli | ENST00000254663; ENSP00000254663; ENSG00000132330 [Q96I15-1] ENST00000409736; ENSP00000387162; ENSG00000132330 [Q96I15-2] |
GeneIDi | 51540 |
KEGGi | hsa:51540 |
UCSCi | uc061uct.1, human [Q96I15-1] |
Organism-specific databases
CTDi | 51540 |
DisGeNETi | 51540 |
EuPathDBi | HostDB:ENSG00000132330.16 |
GeneCardsi | SCLY |
HGNCi | HGNC:18161, SCLY |
HPAi | ENSG00000132330, Low tissue specificity |
MIMi | 611056, gene |
neXtProti | NX_Q96I15 |
OpenTargetsi | ENSG00000132330 |
PharmGKBi | PA134979359 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1549, Eukaryota |
GeneTreei | ENSGT00940000157773 |
HOGENOMi | CLU_003433_10_0_1 |
InParanoidi | Q96I15 |
OrthoDBi | 697150at2759 |
PhylomeDBi | Q96I15 |
TreeFami | TF313550 |
Enzyme and pathway databases
PathwayCommonsi | Q96I15 |
Reactomei | R-HSA-2408508, Metabolism of ingested SeMet, Sec, MeSec into H2Se |
Miscellaneous databases
BioGRID-ORCSi | 51540, 7 hits in 848 CRISPR screens |
ChiTaRSi | SCLY, human |
EvolutionaryTracei | Q96I15 |
GenomeRNAii | 51540 |
Pharosi | Q96I15, Tbio |
PROi | PR:Q96I15 |
RNActi | Q96I15, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000132330, Expressed in right lobe of liver and 130 other tissues |
ExpressionAtlasi | Q96I15, baseline and differential |
Genevisiblei | Q96I15, HS |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR016454, Cysteine_dSase IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00266, Aminotran_5, 1 hit |
PIRSFi | PIRSF005572, NifS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SCLY_HUMAN | |
Accessioni | Q96I15Primary (citable) accession number: Q96I15 Secondary accession number(s): B9A068 Q9NZR7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | July 24, 2013 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations