UniProtKB - Q96HN2 (SAHH3_HUMAN)
Adenosylhomocysteinase 3
AHCYL2
Functioni
Catalytic activityi
- EC:3.3.1.1
Cofactori
: L-homocysteine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.Proteins known to be involved in this subpathway in this organism are:
- Adenosylhomocysteinase (AHCYL1), Adenosylhomocysteinase (AHCYL1), Adenosylhomocysteinase, Adenosylhomocysteinase (AHCY), Adenosylhomocysteinase, Adenosylhomocysteinase (AHCY), Adenosylhomocysteinase (FGFR2-AHCYL1), Adenosylhomocysteinase 3 (AHCYL2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 236 | SubstrateBy similarity | 1 | |
Binding sitei | 310 | SubstrateBy similarity | 1 | |
Binding sitei | 335 | SubstrateBy similarity | 1 | |
Binding sitei | 365 | SubstrateBy similarity | 1 | |
Binding sitei | 369 | SubstrateBy similarity | 1 | |
Binding sitei | 370 | NADBy similarity | 1 | |
Binding sitei | 422 | NAD1 Publication | 1 | |
Binding sitei | 457 | NAD1 Publication | 1 | |
Binding sitei | 525 | NAD1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 336 – 338 | NADBy similarity | 3 | |
Nucleotide bindingi | 401 – 406 | NAD1 Publication | 6 | |
Nucleotide bindingi | 478 – 479 | NAD1 Publication | 2 |
GO - Molecular functioni
- hydrolase activity Source: UniProtKB-KW
GO - Biological processi
- one-carbon metabolic process Source: UniProtKB-KW
- S-adenosylmethionine cycle Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | One-carbon metabolism |
Ligand | NAD |
Enzyme and pathway databases
PathwayCommonsi | Q96HN2 |
Reactomei | R-HSA-425381, Bicarbonate transporters |
UniPathwayi | UPA00314;UER00076 |
Names & Taxonomyi
Protein namesi | Recommended name: Adenosylhomocysteinase 3 (EC:3.3.1.1)Short name: AdoHcyase 3 Alternative name(s): IP(3)Rs binding protein released with IP(3) 2 Short name: IRBIT2 Long-IRBIT1 Publication S-adenosyl-L-homocysteine hydrolase 3 S-adenosylhomocysteine hydrolase-like protein 2 |
Gene namesi | Name:AHCYL2 Synonyms:KIAA0828 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:22204, AHCYL2 |
MIMi | 616520, gene |
neXtProti | NX_Q96HN2 |
VEuPathDBi | HostDB:ENSG00000158467.16 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-KW
Other locations
- neuron projection Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, MicrosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 23382 |
OpenTargetsi | ENSG00000158467 |
PharmGKBi | PA162376046 |
Miscellaneous databases
Pharosi | Q96HN2, Tdark |
Genetic variation databases
BioMutai | AHCYL2 |
DMDMi | 21759427 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000116909 | 2 – 611 | Adenosylhomocysteinase 3Add BLAST | 610 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 107 | PhosphoserineCombined sources | 1 | |
Modified residuei | 149 | Phosphoserine1 Publication | 1 | |
Modified residuei | 152 | Phosphoserine1 Publication | 1 | |
Modified residuei | 155 | Phosphoserine1 Publication | 1 | |
Modified residuei | 158 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q96HN2 |
jPOSTi | Q96HN2 |
MassIVEi | Q96HN2 |
MaxQBi | Q96HN2 |
PaxDbi | Q96HN2 |
PeptideAtlasi | Q96HN2 |
PRIDEi | Q96HN2 |
ProteomicsDBi | 15179 76768 [Q96HN2-1] 76769 [Q96HN2-2] 76770 [Q96HN2-3] |
PTM databases
iPTMneti | Q96HN2 |
PhosphoSitePlusi | Q96HN2 |
Expressioni
Gene expression databases
Bgeei | ENSG00000158467, Expressed in rectum and 215 other tissues |
ExpressionAtlasi | Q96HN2, baseline and differential |
Genevisiblei | Q96HN2, HS |
Organism-specific databases
HPAi | ENSG00000158467, Tissue enhanced (intestine) |
Interactioni
Subunit structurei
Homotetramer (Probable). Forms heteromultimers with AHCYL1 (via the C-terminal region) (PubMed:19220705).
Interacts with ITPR1; with lower affinity than AHCYL1 and maybe via ITPR1 (PubMed:19220705).
Interacts with SLC4A4 (By similarity).
Interacts with ZCCHC4 (PubMed:31799605).
By similarity1 Publication2 PublicationsProtein-protein interaction databases
BioGRIDi | 116958, 93 interactors |
IntActi | Q96HN2, 64 interactors |
STRINGi | 9606.ENSP00000315931 |
Miscellaneous databases
RNActi | Q96HN2, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q96HN2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96HN2 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 109 | LISN domain, inhibits interaction with ITPR11 PublicationAdd BLAST | 108 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1370, Eukaryota |
GeneTreei | ENSGT00950000182981 |
HOGENOMi | CLU_025194_2_1_1 |
InParanoidi | Q96HN2 |
OMAi | QPTHLCE |
OrthoDBi | 371693at2759 |
PhylomeDBi | Q96HN2 |
TreeFami | TF300415 |
Family and domain databases
CDDi | cd00401, SAHH, 1 hit |
Gene3Di | 3.40.50.1480, 2 hits |
InterProi | View protein in InterPro IPR042172, Adenosylhomocyst_ase-like_sf IPR000043, Adenosylhomocysteinase-like IPR015878, Ado_hCys_hydrolase_NAD-bd IPR036291, NAD(P)-bd_dom_sf IPR020082, S-Ado-L-homoCys_hydrolase_CS |
PANTHERi | PTHR23420, PTHR23420, 1 hit |
Pfami | View protein in Pfam PF05221, AdoHcyase, 1 hit PF00670, AdoHcyase_NAD, 1 hit |
SMARTi | View protein in SMART SM00996, AdoHcyase, 1 hit SM00997, AdoHcyase_NAD, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00936, ahcY, 1 hit |
PROSITEi | View protein in PROSITE PS00738, ADOHCYASE_1, 1 hit PS00739, ADOHCYASE_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSVQVVSAAA AAKVPEVELK DLSPSEAESQ LGLSTAAVGA MAPPAGGGDP
60 70 80 90 100
EAPAPAAERP PVPGPGSGPA AALSPAAGKV PQASAMKRSD PHHQHQRHRD
110 120 130 140 150
GGEALVSPDG TVTEAPRTVK KQIQFADQKQ EFNKRPTKIG RRSLSRSISQ
160 170 180 190 200
SSTDSYSSAA SYTDSSDDET SPRDKQQKNS KGSSDFCVKN IKQAEFGRRE
210 220 230 240 250
IEIAEQEMPA LMALRKRAQG EKPLAGAKIV GCTHITAQTA VLMETLGALG
260 270 280 290 300
AQCRWAACNI YSTLNEVAAA LAESGFPVFA WKGESEDDFW WCIDRCVNVE
310 320 330 340 350
GWQPNMILDD GGDLTHWIYK KYPNMFKKIK GIVEESVTGV HRLYQLSKAG
360 370 380 390 400
KLCVPAMNVN DSVTKQKFDN LYCCRESILD GLKRTTDMMF GGKQVVVCGY
410 420 430 440 450
GEVGKGCCAA LKAMGSIVYV TEIDPICALQ ACMDGFRLVK LNEVIRQVDI
460 470 480 490 500
VITCTGNKNV VTREHLDRMK NSCIVCNMGH SNTEIDVASL RTPELTWERV
510 520 530 540 550
RSQVDHVIWP DGKRIVLLAE GRLLNLSCST VPTFVLSITA TTQALALIEL
560 570 580 590 600
YNAPEGRYKQ DVYLLPKKMD EYVASLHLPT FDAHLTELTD EQAKYLGLNK
610
NGPFKPNYYR Y
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0Y8B3 | H0Y8B3_HUMAN | Adenosylhomocysteinase 3 | AHCYL2 | 518 | Annotation score: | ||
C9K0S0 | C9K0S0_HUMAN | Adenosylhomocysteinase 3 | AHCYL2 | 182 | Annotation score: | ||
C9JZV7 | C9JZV7_HUMAN | Adenosylhomocysteinase 3 | AHCYL2 | 75 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046278 | 1 – 19 | MSVQV…PEVEL → MEKWDGNEGTSAFHMPEWM in isoform 4. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_043185 | 1 – 18 | MSVQV…VPEVE → MLGSKKKYIVNGNSGIKA in isoform 3. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_043186 | 19 – 121 | Missing in isoform 3. 1 PublicationAdd BLAST | 103 | |
Alternative sequenceiVSP_046279 | 20 – 122 | Missing in isoform 4. 1 PublicationAdd BLAST | 103 | |
Alternative sequenceiVSP_040095 | 122 | Missing in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB020635 mRNA Translation: BAA74851.1 Different initiation. AK025372 mRNA No translation available. AK295851 mRNA Translation: BAG58657.1 AK316073 mRNA Translation: BAH14444.1 AC009244 Genomic DNA No translation available. AC011005 Genomic DNA No translation available. AC083866 Genomic DNA No translation available. AC093149 Genomic DNA No translation available. BC008349 mRNA Translation: AAH08349.1 BC024325 mRNA Translation: AAH24325.1 |
CCDSi | CCDS47706.1 [Q96HN2-2] CCDS47707.2 [Q96HN2-3] CCDS47708.1 [Q96HN2-4] CCDS5812.1 [Q96HN2-1] |
RefSeqi | NP_001124192.1, NM_001130720.2 [Q96HN2-2] NP_001124194.2, NM_001130722.2 [Q96HN2-3] NP_001124195.1, NM_001130723.2 [Q96HN2-4] NP_056143.1, NM_015328.3 [Q96HN2-1] |
Genome annotation databases
Ensembli | ENST00000325006; ENSP00000315931; ENSG00000158467 [Q96HN2-1] ENST00000446544; ENSP00000413639; ENSG00000158467 [Q96HN2-2] ENST00000474594; ENSP00000420459; ENSG00000158467 [Q96HN2-4] ENST00000490911; ENSP00000420801; ENSG00000158467 [Q96HN2-3] |
GeneIDi | 23382 |
KEGGi | hsa:23382 |
UCSCi | uc003vot.4, human [Q96HN2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB020635 mRNA Translation: BAA74851.1 Different initiation. AK025372 mRNA No translation available. AK295851 mRNA Translation: BAG58657.1 AK316073 mRNA Translation: BAH14444.1 AC009244 Genomic DNA No translation available. AC011005 Genomic DNA No translation available. AC083866 Genomic DNA No translation available. AC093149 Genomic DNA No translation available. BC008349 mRNA Translation: AAH08349.1 BC024325 mRNA Translation: AAH24325.1 |
CCDSi | CCDS47706.1 [Q96HN2-2] CCDS47707.2 [Q96HN2-3] CCDS47708.1 [Q96HN2-4] CCDS5812.1 [Q96HN2-1] |
RefSeqi | NP_001124192.1, NM_001130720.2 [Q96HN2-2] NP_001124194.2, NM_001130722.2 [Q96HN2-3] NP_001124195.1, NM_001130723.2 [Q96HN2-4] NP_056143.1, NM_015328.3 [Q96HN2-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3GVP | X-ray | 2.25 | A/B/C/D | 175-607 | [»] | |
SMRi | Q96HN2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116958, 93 interactors |
IntActi | Q96HN2, 64 interactors |
STRINGi | 9606.ENSP00000315931 |
PTM databases
iPTMneti | Q96HN2 |
PhosphoSitePlusi | Q96HN2 |
Genetic variation databases
BioMutai | AHCYL2 |
DMDMi | 21759427 |
Proteomic databases
EPDi | Q96HN2 |
jPOSTi | Q96HN2 |
MassIVEi | Q96HN2 |
MaxQBi | Q96HN2 |
PaxDbi | Q96HN2 |
PeptideAtlasi | Q96HN2 |
PRIDEi | Q96HN2 |
ProteomicsDBi | 15179 76768 [Q96HN2-1] 76769 [Q96HN2-2] 76770 [Q96HN2-3] |
Protocols and materials databases
Antibodypediai | 17876, 64 antibodies |
Genome annotation databases
Ensembli | ENST00000325006; ENSP00000315931; ENSG00000158467 [Q96HN2-1] ENST00000446544; ENSP00000413639; ENSG00000158467 [Q96HN2-2] ENST00000474594; ENSP00000420459; ENSG00000158467 [Q96HN2-4] ENST00000490911; ENSP00000420801; ENSG00000158467 [Q96HN2-3] |
GeneIDi | 23382 |
KEGGi | hsa:23382 |
UCSCi | uc003vot.4, human [Q96HN2-1] |
Organism-specific databases
CTDi | 23382 |
DisGeNETi | 23382 |
GeneCardsi | AHCYL2 |
HGNCi | HGNC:22204, AHCYL2 |
HPAi | ENSG00000158467, Tissue enhanced (intestine) |
MIMi | 616520, gene |
neXtProti | NX_Q96HN2 |
OpenTargetsi | ENSG00000158467 |
PharmGKBi | PA162376046 |
VEuPathDBi | HostDB:ENSG00000158467.16 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1370, Eukaryota |
GeneTreei | ENSGT00950000182981 |
HOGENOMi | CLU_025194_2_1_1 |
InParanoidi | Q96HN2 |
OMAi | QPTHLCE |
OrthoDBi | 371693at2759 |
PhylomeDBi | Q96HN2 |
TreeFami | TF300415 |
Enzyme and pathway databases
UniPathwayi | UPA00314;UER00076 |
PathwayCommonsi | Q96HN2 |
Reactomei | R-HSA-425381, Bicarbonate transporters |
Miscellaneous databases
BioGRID-ORCSi | 23382, 7 hits in 993 CRISPR screens |
ChiTaRSi | AHCYL2, human |
EvolutionaryTracei | Q96HN2 |
GenomeRNAii | 23382 |
Pharosi | Q96HN2, Tdark |
PROi | PR:Q96HN2 |
RNActi | Q96HN2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000158467, Expressed in rectum and 215 other tissues |
ExpressionAtlasi | Q96HN2, baseline and differential |
Genevisiblei | Q96HN2, HS |
Family and domain databases
CDDi | cd00401, SAHH, 1 hit |
Gene3Di | 3.40.50.1480, 2 hits |
InterProi | View protein in InterPro IPR042172, Adenosylhomocyst_ase-like_sf IPR000043, Adenosylhomocysteinase-like IPR015878, Ado_hCys_hydrolase_NAD-bd IPR036291, NAD(P)-bd_dom_sf IPR020082, S-Ado-L-homoCys_hydrolase_CS |
PANTHERi | PTHR23420, PTHR23420, 1 hit |
Pfami | View protein in Pfam PF05221, AdoHcyase, 1 hit PF00670, AdoHcyase_NAD, 1 hit |
SMARTi | View protein in SMART SM00996, AdoHcyase, 1 hit SM00997, AdoHcyase_NAD, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00936, ahcY, 1 hit |
PROSITEi | View protein in PROSITE PS00738, ADOHCYASE_1, 1 hit PS00739, ADOHCYASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SAHH3_HUMAN | |
Accessioni | Q96HN2Primary (citable) accession number: Q96HN2 Secondary accession number(s): B4DIZ5, D9N155, O94917 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2002 |
Last sequence update: | December 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families