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Protein

Protein N-terminal glutamine amidohydrolase

Gene

WDYHV1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.By similarity

Catalytic activityi

N-terminal L-glutaminyl-[protein] + H2O = N-terminal L-glutamyl-[protein] + NH3.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei28By similarity1
Active sitei81By similarity1
Active sitei97By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000156795-MONOMER

Protein family/group databases

MoonDBiQ96HA8 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-terminal glutamine amidohydrolase (EC:3.5.1.122By similarity)
Alternative name(s):
Protein NH2-terminal glutamine deamidase
Short name:
N-terminal Gln amidase
Short name:
Nt(Q)-amidase
WDYHV motif-containing protein 1
Gene namesi
Name:WDYHV1
Synonyms:C8orf32, NTAQ1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000156795.6
HGNCiHGNC:25490 WDYHV1
neXtProtiNX_Q96HA8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000156795
PharmGKBiPA164727566

Polymorphism and mutation databases

BioMutaiWDYHV1
DMDMi152112225

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002794091 – 205Protein N-terminal glutamine amidohydrolaseAdd BLAST205

Proteomic databases

EPDiQ96HA8
MaxQBiQ96HA8
PaxDbiQ96HA8
PeptideAtlasiQ96HA8
PRIDEiQ96HA8
ProteomicsDBi76725

PTM databases

iPTMnetiQ96HA8
PhosphoSitePlusiQ96HA8

Expressioni

Gene expression databases

BgeeiENSG00000156795 Expressed in 214 organ(s), highest expression level in heart
CleanExiHS_WDYHV1
ExpressionAtlasiQ96HA8 baseline and differential
GenevisibleiQ96HA8 HS

Organism-specific databases

HPAiHPA024823
HPA053680

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTBP607093EBI-741158,EBI-353944
ACTG1P632615EBI-741158,EBI-351292
AESQ08117-24EBI-741158,EBI-11741437
AMOTQ4VCS5-23EBI-741158,EBI-3891843
AMOTL2Q9Y2J4-43EBI-741158,EBI-10187270
APIPQ96GX93EBI-741158,EBI-359248
ASLP044245EBI-741158,EBI-750131
BIRC2Q134903EBI-741158,EBI-514538
BLMHQ138673EBI-741158,EBI-718504
C1orf50Q9BV195EBI-741158,EBI-2874661
CAPN3P208073EBI-741158,EBI-5655000
CCDC102BQ68D863EBI-741158,EBI-10171570
CCDC184Q52MB23EBI-741158,EBI-10179526
CDAP323205EBI-741158,EBI-9250559
CDR2Q018503EBI-741158,EBI-1181367
CEP55Q53EZ44EBI-741158,EBI-747776
COG6Q9Y2V74EBI-741158,EBI-3866319
COILP384323EBI-741158,EBI-945751
CRYAAP024895EBI-741158,EBI-6875961
CTHP329293EBI-741158,EBI-749763
DAB1O755533EBI-741158,EBI-7875264
DCTPP1Q9H7733EBI-741158,EBI-723569
DHPSP493663EBI-741158,EBI-741925
EDARADDQ8WWZ33EBI-741158,EBI-2949647
EIF2B1Q142325EBI-741158,EBI-491065
ETV6P412123EBI-741158,EBI-1372759
FTH1Q6NZ443EBI-741158,EBI-10180219
GAS7O608613EBI-741158,EBI-2683717
GAS7O60861-14EBI-741158,EBI-11745923
GMDSO605475EBI-741158,EBI-746373
GOLGA2Q083797EBI-741158,EBI-618309
HEL-S-182mPV9HW603EBI-741158,EBI-10180762
HEL-S-70V9HW803EBI-741158,EBI-10175326
HPRT1P004928EBI-741158,EBI-748210
HSD17B14Q9BPX110EBI-741158,EBI-742664
JUPP149233EBI-741158,EBI-702484
KCNH1O952593EBI-741158,EBI-2909270
KCTD1Q719H93EBI-741158,EBI-9027502
KLHL12Q53G597EBI-741158,EBI-740929
KRT31Q153233EBI-741158,EBI-948001
KRTAP10-5P603703EBI-741158,EBI-10172150
KRTAP10-7P604093EBI-741158,EBI-10172290
KRTAP4-2Q9BYR53EBI-741158,EBI-10172511
KRTAP5-9P263715EBI-741158,EBI-3958099
KRTAP9-2Q9BYQ45EBI-741158,EBI-1044640
KRTAP9-4Q9BYQ23EBI-741158,EBI-10185730
LONRF1Q17RB83EBI-741158,EBI-2341787
LZTFL1Q9NQ483EBI-741158,EBI-2824799
LZTS2Q9BRK43EBI-741158,EBI-741037
MAGEA11P43364-23EBI-741158,EBI-10178634
MARCH10Q8NA823EBI-741158,EBI-2341554
MIFI4AY873EBI-741158,EBI-10287234
MTUS2Q5JR593EBI-741158,EBI-742948
MTUS2Q5JR59-34EBI-741158,EBI-11522433
NAGKQ9UJ70-24EBI-741158,EBI-11526455
NECAB2Q7Z6G3-27EBI-741158,EBI-10172876
NME1P155315EBI-741158,EBI-741141
NPLQ9BXD53EBI-741158,EBI-10287915
NT5C1AQ9BXI34EBI-741158,EBI-10441581
NUDT14O958483EBI-741158,EBI-536866
PCBD1P614573EBI-741158,EBI-740475
PNMA1Q8ND905EBI-741158,EBI-302345
PNMA5Q96PV45EBI-741158,EBI-10171633
PPCDCQ96CD25EBI-741158,EBI-724333
PRMT1Q998733EBI-741158,EBI-78738
PRPS2P119083EBI-741158,EBI-4290895
PS1TP5BP1Q1KLZ03EBI-741158,EBI-9978131
PSME3P612894EBI-741158,EBI-355546
PYGMP112173EBI-741158,EBI-357469
RABAC1Q9UI148EBI-741158,EBI-712367
RAD54LQ926983EBI-741158,EBI-5333483
RBPMSQ930623EBI-741158,EBI-740322
ROPN1Q9HAT04EBI-741158,EBI-1378139
RPIAP492476EBI-741158,EBI-744831
SEPT3Q9UH033EBI-741158,EBI-727037
SFNP319473EBI-741158,EBI-476295
SIAH1Q8IUQ43EBI-741158,EBI-747107
SPERTQ8NA61-24EBI-741158,EBI-11524851
STX11O755583EBI-741158,EBI-714135
TCL1AP562794EBI-741158,EBI-749995
THAP1Q9NVV96EBI-741158,EBI-741515
TMEM239Q8WW343EBI-741158,EBI-9675724
TNRA1L3063EBI-741158,EBI-10182881
TOLLIPQ9H0E23EBI-741158,EBI-74615
TRIM27P143735EBI-741158,EBI-719493
TRIM54Q9BYV25EBI-741158,EBI-2130429
TRIP13Q156453EBI-741158,EBI-358993
TSC22D1Q157143EBI-741158,EBI-712609
VAC14Q08AM63EBI-741158,EBI-2107455
VPS37CA5D8V64EBI-741158,EBI-2559305
XIAPP981703EBI-741158,EBI-517127
ZBTB8AQ96BR93EBI-741158,EBI-742740
ZNF341Q9BYN73EBI-741158,EBI-9089622

Protein-protein interaction databases

BioGridi120405, 135 interactors
IntActiQ96HA8, 374 interactors
MINTiQ96HA8
STRINGi9606.ENSP00000287387

Structurei

Secondary structure

1205
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96HA8
SMRiQ96HA8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96HA8

Family & Domainsi

Sequence similaritiesi

Belongs to the NTAQ1 family.Curated

Phylogenomic databases

eggNOGiKOG3261 Eukaryota
ENOG4111G3S LUCA
GeneTreeiENSGT00390000014398
HOGENOMiHOG000007890
HOVERGENiHBG102197
InParanoidiQ96HA8
KOiK21286
OMAiVHRFGSQ
OrthoDBiEOG091G0K1H
PhylomeDBiQ96HA8
TreeFamiTF105807

Family and domain databases

Gene3Di3.10.620.10, 1 hit
InterProiView protein in InterPro
IPR037132 N_Gln_amidohydro_ab_roll_sf
IPR039733 NTAQ1
IPR023128 Prot_N_Gln_amidohydro_ab_roll
PANTHERiPTHR13035 PTHR13035, 1 hit
PfamiView protein in Pfam
PF09764 Nt_Gln_amidase, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96HA8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEGNGPAAVH YQPASPPRDA CVYSSCYCEE NIWKLCEYIK NHDQYPLEEC
60 70 80 90 100
YAVFISNERK MIPIWKQQAR PGDGPVIWDY HVVLLHVSSG GQNFIYDLDT
110 120 130 140 150
VLPFPCLFDT YVEDAFKSDD DIHPQFRRKF RVIRADSYLK NFASDRSHMK
160 170 180 190 200
DSSGNWREPP PPYPCIETGD SKMNLNDFIS MDPKVGWGAV YTLSEFTHRF

GSKNC
Length:205
Mass (Da):23,680
Last modified:March 6, 2007 - v2
Checksum:iF858AB0C73928CA9
GO
Isoform 2 (identifier: Q96HA8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.

Note: No experimental confirmation available.
Show »
Length:145
Mass (Da):16,736
Checksum:iAC61BBE11D3642F1
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RHC2E5RHC2_HUMAN
Protein N-terminal glutamine amidoh...
WDYHV1
196Annotation score:
E5RIY9E5RIY9_HUMAN
Protein N-terminal glutamine amidoh...
WDYHV1
57Annotation score:
E9PRB7E9PRB7_HUMAN
Protein N-terminal glutamine amidoh...
WDYHV1
87Annotation score:
H0YBV4H0YBV4_HUMAN
Protein N-terminal glutamine amidoh...
WDYHV1
63Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03088232I → V1 PublicationCorresponds to variant dbSNP:rs6999234Ensembl.1
Natural variantiVAR_03088393N → S1 PublicationCorresponds to variant dbSNP:rs7014678Ensembl.1
Natural variantiVAR_030884116F → I1 PublicationCorresponds to variant dbSNP:rs6470147Ensembl.1
Natural variantiVAR_030885134R → C1 PublicationCorresponds to variant dbSNP:rs3824250Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0552681 – 60Missing in isoform 2. 1 PublicationAdd BLAST60

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001066 mRNA Translation: BAA91488.1
AK293492 mRNA Translation: BAG56979.1
AC021305 Genomic DNA No translation available.
CH471060 Genomic DNA Translation: EAW92038.1
BC008781 mRNA Translation: AAH08781.1
CCDSiCCDS6344.1 [Q96HA8-1]
CCDS64965.1 [Q96HA8-2]
RefSeqiNP_001269953.1, NM_001283024.1 [Q96HA8-2]
NP_060494.1, NM_018024.2 [Q96HA8-1]
UniGeneiHs.18029

Genome annotation databases

EnsembliENST00000287387; ENSP00000287387; ENSG00000156795 [Q96HA8-1]
ENST00000523984; ENSP00000430427; ENSG00000156795 [Q96HA8-2]
GeneIDi55093
KEGGihsa:55093
UCSCiuc003yqn.3 human [Q96HA8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001066 mRNA Translation: BAA91488.1
AK293492 mRNA Translation: BAG56979.1
AC021305 Genomic DNA No translation available.
CH471060 Genomic DNA Translation: EAW92038.1
BC008781 mRNA Translation: AAH08781.1
CCDSiCCDS6344.1 [Q96HA8-1]
CCDS64965.1 [Q96HA8-2]
RefSeqiNP_001269953.1, NM_001283024.1 [Q96HA8-2]
NP_060494.1, NM_018024.2 [Q96HA8-1]
UniGeneiHs.18029

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C9QX-ray1.50A2-205[»]
4W79X-ray1.50A2-202[»]
ProteinModelPortaliQ96HA8
SMRiQ96HA8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120405, 135 interactors
IntActiQ96HA8, 374 interactors
MINTiQ96HA8
STRINGi9606.ENSP00000287387

Protein family/group databases

MoonDBiQ96HA8 Predicted

PTM databases

iPTMnetiQ96HA8
PhosphoSitePlusiQ96HA8

Polymorphism and mutation databases

BioMutaiWDYHV1
DMDMi152112225

Proteomic databases

EPDiQ96HA8
MaxQBiQ96HA8
PaxDbiQ96HA8
PeptideAtlasiQ96HA8
PRIDEiQ96HA8
ProteomicsDBi76725

Protocols and materials databases

DNASUi55093
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000287387; ENSP00000287387; ENSG00000156795 [Q96HA8-1]
ENST00000523984; ENSP00000430427; ENSG00000156795 [Q96HA8-2]
GeneIDi55093
KEGGihsa:55093
UCSCiuc003yqn.3 human [Q96HA8-1]

Organism-specific databases

CTDi55093
EuPathDBiHostDB:ENSG00000156795.6
GeneCardsiWDYHV1
HGNCiHGNC:25490 WDYHV1
HPAiHPA024823
HPA053680
neXtProtiNX_Q96HA8
OpenTargetsiENSG00000156795
PharmGKBiPA164727566
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3261 Eukaryota
ENOG4111G3S LUCA
GeneTreeiENSGT00390000014398
HOGENOMiHOG000007890
HOVERGENiHBG102197
InParanoidiQ96HA8
KOiK21286
OMAiVHRFGSQ
OrthoDBiEOG091G0K1H
PhylomeDBiQ96HA8
TreeFamiTF105807

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000156795-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ96HA8
GeneWikiiC8orf32
GenomeRNAii55093
PROiPR:Q96HA8

Gene expression databases

BgeeiENSG00000156795 Expressed in 214 organ(s), highest expression level in heart
CleanExiHS_WDYHV1
ExpressionAtlasiQ96HA8 baseline and differential
GenevisibleiQ96HA8 HS

Family and domain databases

Gene3Di3.10.620.10, 1 hit
InterProiView protein in InterPro
IPR037132 N_Gln_amidohydro_ab_roll_sf
IPR039733 NTAQ1
IPR023128 Prot_N_Gln_amidohydro_ab_roll
PANTHERiPTHR13035 PTHR13035, 1 hit
PfamiView protein in Pfam
PF09764 Nt_Gln_amidase, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNTAQ1_HUMAN
AccessioniPrimary (citable) accession number: Q96HA8
Secondary accession number(s): B4DE68, Q9NW95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: November 7, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
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