Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable ATP-dependent RNA helicase DDX27

Gene

DDX27

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA (PubMed:25825154).1 Publication

Caution

It is uncertain whether Met-1 or Met-32 is the initiator.Curated

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi262 – 269ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • rRNA processing Source: UniProtKB

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processRibosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX27 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 27
Gene namesi
Name:DDX27
Synonyms:cPERP-F1 Publication, RHLP
ORF Names:HSPC259, PP3241
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000124228.14
HGNCiHGNC:15837 DDX27
MIMi616621 gene
neXtProtiNX_Q96GQ7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi86 – 88Missing : No interaction with PEBOW complex. 1 Publication3

Organism-specific databases

DisGeNETi55661
OpenTargetsiENSG00000124228
PharmGKBiPA27213

Polymorphism and mutation databases

BioMutaiDDX27
DMDMi29427946

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550311 – 796Probable ATP-dependent RNA helicase DDX27Add BLAST796

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54PhosphoserineBy similarity1
Modified residuei56PhosphoserineBy similarity1
Modified residuei79PhosphoserineCombined sources1
Modified residuei166PhosphoserineCombined sources1
Modified residuei177PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96GQ7
MaxQBiQ96GQ7
PaxDbiQ96GQ7
PeptideAtlasiQ96GQ7
PRIDEiQ96GQ7
ProteomicsDBi76655

2D gel databases

SWISS-2DPAGEiQ96GQ7

PTM databases

iPTMnetiQ96GQ7
PhosphoSitePlusiQ96GQ7
SwissPalmiQ96GQ7

Expressioni

Gene expression databases

BgeeiENSG00000124228 Expressed in 234 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_DDX27
ExpressionAtlasiQ96GQ7 baseline and differential
GenevisibleiQ96GQ7 HS

Organism-specific databases

HPAiHPA047087

Interactioni

Subunit structurei

Associates with PeBoW complex, composed of BOP1, PES1 and WDR12 (PubMed:25825154). Interacts directly with BOP1 and PES1 (PubMed:25825154).1 Publication

Protein-protein interaction databases

BioGridi120793, 113 interactors
CORUMiQ96GQ7
IntActiQ96GQ7, 22 interactors
MINTiQ96GQ7
STRINGi9606.ENSP00000360828

Structurei

3D structure databases

ProteinModelPortaliQ96GQ7
SMRiQ96GQ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini249 – 423Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST175
Domaini457 – 603Helicase C-terminalPROSITE-ProRule annotationAdd BLAST147

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi86 – 88Required for interaction with the PEBOW complex1 Publication3
Motifi195 – 200Nuclear localization signalSequence analysis6
Motifi218 – 246Q motifAdd BLAST29
Motifi371 – 374DEAD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi44 – 200Asp/Glu/Lys-richAdd BLAST157

Domaini

The C-terminal domain regulates nucleolar localization (PubMed:25825154).1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0338 Eukaryota
COG0513 LUCA
GeneTreeiENSGT00550000074997
HOVERGENiHBG106162
InParanoidiQ96GQ7
KOiK13181
OrthoDBiEOG091G048O
PhylomeDBiQ96GQ7
TreeFamiTF314780

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q96GQ7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVLAQRRRGG CEKLRAGPQA VLASGSGFCD NMLADLGLIG TIGEDDEVPV
60 70 80 90 100
EPESDSGDEE EEGPIVLGRR QKALGKNRSA DFNPDFVFTE KEGTYDGSWA
110 120 130 140 150
LADVMSQLKK KRAATTLDEK IEKVRKKRKT EDKEAKSGKL EKEKEAKEGS
160 170 180 190 200
EPKEQEDLQE NDEEGSEDEA SETDYSSADE NILTKADTLK VKDRKKKKKK
210 220 230 240 250
GQEAGGFFED ASQYDENLSF QDMNLSRPLL KAITAMGFKQ PTPIQKACIP
260 270 280 290 300
VGLLGKDICA CAATGTGKTA AFALPVLERL IYKPRQAPVT RVLVLVPTRE
310 320 330 340 350
LGIQVHSVTR QLAQFCNITT CLAVGGLDVK SQEAALRAAP DILIATPGRL
360 370 380 390 400
IDHLHNCPSF HLSSIEVLIL DEADRMLDEY FEEQMKEIIR MCSHHRQTML
410 420 430 440 450
FSATMTDEVK DLASVSLKNP VRIFVNSNTD VAPFLRQEFI RIRPNREGDR
460 470 480 490 500
EAIVAALLTR TFTDHVMLFT QTKKQAHRMH ILLGLMGLQV GELHGNLSQT
510 520 530 540 550
QRLEALRRFK DEQIDILVAT DVAARGLDIE GVKTVINFTM PNTIKHYVHR
560 570 580 590 600
VGRTARAGRA GRSVSLVGED ERKMLKEIVK AAKAPVKARI LPQDVILKFR
610 620 630 640 650
DKIEKMEKDV YAVLQLEAEE KEMQQSEAQI NTAKRLLEKG KEAVVQEPER
660 670 680 690 700
SWFQTKEERK KEKIAKALQE FDLALRGKKK RKKFMKDAKK KGEMTAEERS
710 720 730 740 750
QFEILKAQMF AERLAKRNRR AKRARAMPEE EPVRGPAKKQ KQGKKSVFDE
760 770 780 790
ELTNTSKKAL KQYRAGPSFE ERKQLGLPHQ RRGGNFKSKS RYKRRK
Length:796
Mass (Da):89,835
Last modified:March 28, 2003 - v2
Checksum:i9282C712B8F8B84A
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7Z6D5B7Z6D5_HUMAN
Probable ATP-dependent RNA helicase...
DDX27
765Annotation score:
A0A087X059A0A087X059_HUMAN
Probable ATP-dependent RNA helicase...
DDX27
275Annotation score:
A0A087WYH5A0A087WYH5_HUMAN
Probable ATP-dependent RNA helicase...
DDX27
169Annotation score:

Sequence cautioni

The sequence AAF28937 differs from that shown. Sequencing errors.Curated
The sequence AAF28937 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence AAG22482 differs from that shown. Reason: Frameshift at positions 208, 427, 434 and 532.Curated
The sequence AAH16060 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA91284 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14343 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti482L → F in AAK21271 (Ref. 1) Curated1
Sequence conflicti542N → S in BAA91284 (PubMed:14702039).Curated1
Sequence conflicti548V → D in AAK95821 (Ref. 1) Curated1
Sequence conflicti548V → D in BAB14343 (PubMed:14702039).Curated1
Sequence conflicti674A → T in AAK21271 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_022849766G → S2 PublicationsCorresponds to variant dbSNP:rs1130146Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY044431 mRNA Translation: AAK95821.1
AF336851 mRNA Translation: AAK21271.1
AL049766 Genomic DNA No translation available.
AL357560 Genomic DNA No translation available.
BC009304 mRNA Translation: AAH09304.2
BC011927 mRNA Translation: AAH11927.2
BC016060 mRNA Translation: AAH16060.2 Different initiation.
BC126287 mRNA Translation: AAI26288.1
BC130275 mRNA Translation: AAI30276.1
BC144125 mRNA Translation: AAI44126.1
AK022979 mRNA Translation: BAB14343.1 Different initiation.
AK000603 mRNA Translation: BAA91284.1 Different initiation.
AF193054 mRNA Translation: AAG22482.1 Frameshift.
AF161377 mRNA Translation: AAF28937.1 Sequence problems.
CCDSiCCDS13416.1
RefSeqiNP_060365.7, NM_017895.7
UniGeneiHs.129261
Hs.65234

Genome annotation databases

EnsembliENST00000371764; ENSP00000360828; ENSG00000124228
ENST00000622530; ENSP00000483495; ENSG00000124228
GeneIDi55661
KEGGihsa:55661
UCSCiuc002xuh.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY044431 mRNA Translation: AAK95821.1
AF336851 mRNA Translation: AAK21271.1
AL049766 Genomic DNA No translation available.
AL357560 Genomic DNA No translation available.
BC009304 mRNA Translation: AAH09304.2
BC011927 mRNA Translation: AAH11927.2
BC016060 mRNA Translation: AAH16060.2 Different initiation.
BC126287 mRNA Translation: AAI26288.1
BC130275 mRNA Translation: AAI30276.1
BC144125 mRNA Translation: AAI44126.1
AK022979 mRNA Translation: BAB14343.1 Different initiation.
AK000603 mRNA Translation: BAA91284.1 Different initiation.
AF193054 mRNA Translation: AAG22482.1 Frameshift.
AF161377 mRNA Translation: AAF28937.1 Sequence problems.
CCDSiCCDS13416.1
RefSeqiNP_060365.7, NM_017895.7
UniGeneiHs.129261
Hs.65234

3D structure databases

ProteinModelPortaliQ96GQ7
SMRiQ96GQ7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120793, 113 interactors
CORUMiQ96GQ7
IntActiQ96GQ7, 22 interactors
MINTiQ96GQ7
STRINGi9606.ENSP00000360828

PTM databases

iPTMnetiQ96GQ7
PhosphoSitePlusiQ96GQ7
SwissPalmiQ96GQ7

Polymorphism and mutation databases

BioMutaiDDX27
DMDMi29427946

2D gel databases

SWISS-2DPAGEiQ96GQ7

Proteomic databases

EPDiQ96GQ7
MaxQBiQ96GQ7
PaxDbiQ96GQ7
PeptideAtlasiQ96GQ7
PRIDEiQ96GQ7
ProteomicsDBi76655

Protocols and materials databases

DNASUi55661
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371764; ENSP00000360828; ENSG00000124228
ENST00000622530; ENSP00000483495; ENSG00000124228
GeneIDi55661
KEGGihsa:55661
UCSCiuc002xuh.4 human

Organism-specific databases

CTDi55661
DisGeNETi55661
EuPathDBiHostDB:ENSG00000124228.14
GeneCardsiDDX27
HGNCiHGNC:15837 DDX27
HPAiHPA047087
MIMi616621 gene
neXtProtiNX_Q96GQ7
OpenTargetsiENSG00000124228
PharmGKBiPA27213
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0338 Eukaryota
COG0513 LUCA
GeneTreeiENSGT00550000074997
HOVERGENiHBG106162
InParanoidiQ96GQ7
KOiK13181
OrthoDBiEOG091G048O
PhylomeDBiQ96GQ7
TreeFamiTF314780

Miscellaneous databases

ChiTaRSiDDX27 human
GeneWikiiDDX27
GenomeRNAii55661
PROiPR:Q96GQ7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000124228 Expressed in 234 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_DDX27
ExpressionAtlasiQ96GQ7 baseline and differential
GenevisibleiQ96GQ7 HS

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDDX27_HUMAN
AccessioniPrimary (citable) accession number: Q96GQ7
Secondary accession number(s): A0AVB6
, B7ZLY1, Q5VXM7, Q8WYG4, Q969N7, Q96F57, Q96L97, Q9BWY9, Q9BXF0, Q9H990, Q9NWU3, Q9P0C2, Q9UGD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: November 7, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again