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Protein

DCN1-like protein 1

Gene

DCUN1D1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (PubMed:25349211, PubMed:28581483). Acts also as an oncogene facilitating malignant transformation and carcinogenic progression (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei115Essential for interaction with UBE2M1 Publication1

GO - Molecular functioni

  • cullin family protein binding Source: GO_Central
  • ubiquitin conjugating enzyme binding Source: GO_Central
  • ubiquitin-like protein binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000043093-MONOMER
ReactomeiR-HSA-8951664 Neddylation

Names & Taxonomyi

Protein namesi
Recommended name:
DCN1-like protein 1
Alternative name(s):
DCUN1 domain-containing protein 1
Defective in cullin neddylation protein 1-like protein 1
Squamous cell carcinoma-related oncogene
Gene namesi
Name:DCUN1D1
Synonyms:DCN11 Publication, DCUN1L1, RP42, SCCRO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000043093.13
HGNCiHGNC:18184 DCUN1D1
MIMi605905 gene
neXtProtiNX_Q96GG9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi115C → A, G, I, L, T or V: Loss of ability to stimulate cullin neddylation. 1 Publication1
Mutagenesisi241D → N: Loss of binding to CAND1 and CUL-RBX1 complex but retains binding to UBE2M. 1 Publication1

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi54165
OpenTargetsiENSG00000043093
PharmGKBiPA142672008

Polymorphism and mutation databases

BioMutaiDCUN1D1
DMDMi73919222

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001294981 – 259DCN1-like protein 1Add BLAST259

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ96GG9
MaxQBiQ96GG9
PaxDbiQ96GG9
PeptideAtlasiQ96GG9
PRIDEiQ96GG9
ProteomicsDBi76634

PTM databases

iPTMnetiQ96GG9
PhosphoSitePlusiQ96GG9

Expressioni

Tissue specificityi

Expressed in pancreas, kidney, placenta, brain and heart. Weakly or not expressed in liver, skeletal muscle and lung. Strongly overexpressed in thyroid tumors, bronchioloalveolar carcinomas, and malignant tissues of squamous cell carcinoma of the oral tongue. Not overexpressed in aggressive adrenocortical carcinomas.1 Publication

Gene expression databases

BgeeiENSG00000043093 Expressed in 228 organ(s), highest expression level in right testis
CleanExiHS_DCUN1D1
ExpressionAtlasiQ96GG9 baseline and differential
GenevisibleiQ96GG9 HS

Organism-specific databases

HPAiHPA035911

Interactioni

Subunit structurei

Part of an E3 complex for neddylation composed of cullins, RBX1, UBE2M and CAND1. Interacts (via the C-terminus 50 AA) with CUL1, CUL2, CUL3, CUL4 and CUL5. Binds neddylated CUL1. Interacts (via the C-terminus 50 AA) directly with RBX1 (PubMed:18826954). Interacts (via DCUN1 domain) with UBE2M (acetylated at N-terminal methionine) (PubMed:28581483). Interacts preferentially with UBE2M-NEDD8 thioester (via N-terminus 1-26 AA) than with free UBE2M (PubMed:18826954, PubMed:25349211). UBE2M N-terminal acetylation increases the affinity of this interaction by about 2 orders of magnitude (PubMed:21940857). Interacts with CAND1 when in complex with CUL1-RBX1 (PubMed:18826954).4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119920, 237 interactors
DIPiDIP-42121N
ELMiQ96GG9
IntActiQ96GG9, 31 interactors
MINTiQ96GG9
STRINGi9606.ENSP00000292782

Structurei

Secondary structure

1259
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96GG9
SMRiQ96GG9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 45UBA-likeAdd BLAST38
Domaini60 – 248DCUN1PROSITE-ProRule annotationAdd BLAST189

Domaini

The DCUN1 domain, also known as PONY domain mediates its interaction with N-terminally acetylated UBE2M.1 Publication

Phylogenomic databases

eggNOGiKOG3077 Eukaryota
ENOG410XTIJ LUCA
GeneTreeiENSGT00550000074675
HOGENOMiHOG000241761
HOVERGENiHBG055256
InParanoidiQ96GG9
KOiK17822
OMAiWPSVIDE
OrthoDBiEOG091G0HHH
PhylomeDBiQ96GG9
TreeFamiTF313332

Family and domain databases

InterProiView protein in InterPro
IPR014764 DCN-prot
IPR005176 PONY_dom
IPR009060 UBA-like_sf
PANTHERiPTHR12281 PTHR12281, 1 hit
PfamiView protein in Pfam
PF03556 Cullin_binding, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
PROSITEiView protein in PROSITE
PS51229 DCUN1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q96GG9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNKLKSSQKD KVRQFMIFTQ SSEKTAVSCL SQNDWKLDVA TDNFFQNPEL
60 70 80 90 100
YIRESVKGSL DRKKLEQLYN RYKDPQDENK IGIDGIQQFC DDLALDPASI
110 120 130 140 150
SVLIIAWKFR AATQCEFSKQ EFMDGMTELG CDSIEKLKAQ IPKMEQELKE
160 170 180 190 200
PGRFKDFYQF TFNFAKNPGQ KGLDLEMAIA YWNLVLNGRF KFLDLWNKFL
210 220 230 240 250
LEHHKRSIPK DTWNLLLDFS TMIADDMSNY DEEGAWPVLI DDFVEFARPQ

IAGTKSTTV
Length:259
Mass (Da):30,124
Last modified:December 1, 2001 - v1
Checksum:iF3709235E0610C54
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JVE2C9JVE2_HUMAN
DCN1-like protein
DCUN1D1 hCG_17206
244Annotation score:
C9J8R4C9J8R4_HUMAN
DCN1-like protein
DCUN1D1
128Annotation score:
C9JRU6C9JRU6_HUMAN
DCN1-like protein
DCUN1D1
75Annotation score:
C9JUW4C9JUW4_HUMAN
DCN1-like protein
DCUN1D1
98Annotation score:
C9J0B2C9J0B2_HUMAN
DCN1-like protein
DCUN1D1
92Annotation score:
F8WC32F8WC32_HUMAN
DCN1-like protein 1
DCUN1D1
35Annotation score:

Sequence cautioni

The sequence BAB15235 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134I → T in AAL78673 (PubMed:17018598).Curated1
Sequence conflicti136K → Q in AAG00606 (PubMed:10777668).Curated1
Sequence conflicti191 – 192KF → RL in AAL78673 (PubMed:17018598).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF292100 mRNA Translation: AAG00606.2
AF456425 mRNA Translation: AAL78672.1
AF456426 mRNA Translation: AAL78673.1
AK025764 mRNA Translation: BAB15235.1 Different initiation.
AK056335 mRNA Translation: BAG51680.1
AK314480 mRNA Translation: BAG37084.1
CH471052 Genomic DNA Translation: EAW78342.1
BC009478 mRNA Translation: AAH09478.1
BC013163 mRNA Translation: AAH13163.2
CCDSiCCDS3240.1
RefSeqiNP_001295030.1, NM_001308101.1
NP_065691.2, NM_020640.3
UniGeneiHs.592789
Hs.744153

Genome annotation databases

EnsembliENST00000292782; ENSP00000292782; ENSG00000043093
GeneIDi54165
KEGGihsa:54165
UCSCiuc003fld.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF292100 mRNA Translation: AAG00606.2
AF456425 mRNA Translation: AAL78672.1
AF456426 mRNA Translation: AAL78673.1
AK025764 mRNA Translation: BAB15235.1 Different initiation.
AK056335 mRNA Translation: BAG51680.1
AK314480 mRNA Translation: BAG37084.1
CH471052 Genomic DNA Translation: EAW78342.1
BC009478 mRNA Translation: AAH09478.1
BC013163 mRNA Translation: AAH13163.2
CCDSiCCDS3240.1
RefSeqiNP_001295030.1, NM_001308101.1
NP_065691.2, NM_020640.3
UniGeneiHs.592789
Hs.744153

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TDUX-ray1.50A/B62-259[»]
3TDZX-ray2.00A/B62-259[»]
4P5OX-ray3.11E/F62-259[»]
5UFIX-ray2.58A/B/C/D58-259[»]
5V83X-ray2.00A62-259[»]
5V86X-ray1.37A62-259[»]
5V88X-ray1.60A62-259[»]
6B5QX-ray2.16A/B58-259[»]
6BG3X-ray1.05A62-259[»]
6BG5X-ray1.10A62-259[»]
ProteinModelPortaliQ96GG9
SMRiQ96GG9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119920, 237 interactors
DIPiDIP-42121N
ELMiQ96GG9
IntActiQ96GG9, 31 interactors
MINTiQ96GG9
STRINGi9606.ENSP00000292782

PTM databases

iPTMnetiQ96GG9
PhosphoSitePlusiQ96GG9

Polymorphism and mutation databases

BioMutaiDCUN1D1
DMDMi73919222

Proteomic databases

EPDiQ96GG9
MaxQBiQ96GG9
PaxDbiQ96GG9
PeptideAtlasiQ96GG9
PRIDEiQ96GG9
ProteomicsDBi76634

Protocols and materials databases

DNASUi54165
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292782; ENSP00000292782; ENSG00000043093
GeneIDi54165
KEGGihsa:54165
UCSCiuc003fld.2 human

Organism-specific databases

CTDi54165
DisGeNETi54165
EuPathDBiHostDB:ENSG00000043093.13
GeneCardsiDCUN1D1
HGNCiHGNC:18184 DCUN1D1
HPAiHPA035911
MIMi605905 gene
neXtProtiNX_Q96GG9
OpenTargetsiENSG00000043093
PharmGKBiPA142672008
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3077 Eukaryota
ENOG410XTIJ LUCA
GeneTreeiENSGT00550000074675
HOGENOMiHOG000241761
HOVERGENiHBG055256
InParanoidiQ96GG9
KOiK17822
OMAiWPSVIDE
OrthoDBiEOG091G0HHH
PhylomeDBiQ96GG9
TreeFamiTF313332

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000043093-MONOMER
ReactomeiR-HSA-8951664 Neddylation

Miscellaneous databases

ChiTaRSiDCUN1D1 human
GeneWikiiDCUN1D1
GenomeRNAii54165
PROiPR:Q96GG9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000043093 Expressed in 228 organ(s), highest expression level in right testis
CleanExiHS_DCUN1D1
ExpressionAtlasiQ96GG9 baseline and differential
GenevisibleiQ96GG9 HS

Family and domain databases

InterProiView protein in InterPro
IPR014764 DCN-prot
IPR005176 PONY_dom
IPR009060 UBA-like_sf
PANTHERiPTHR12281 PTHR12281, 1 hit
PfamiView protein in Pfam
PF03556 Cullin_binding, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
PROSITEiView protein in PROSITE
PS51229 DCUN1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDCNL1_HUMAN
AccessioniPrimary (citable) accession number: Q96GG9
Secondary accession number(s): B2RB37
, Q7L3G9, Q8TEX7, Q9H6M1, Q9HCT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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