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Protein

E3 ubiquitin-protein transferase RMND5B

Gene

RMND5B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972). Catalytic activity of the complex is required for normal cell proliferation (PubMed:29911972). The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (PubMed:29911972).1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri338 – 379RING-Gid-typePROSITE-ProRule annotationAdd BLAST42

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein transferase RMND5B (EC:2.3.2.271 Publication)
Alternative name(s):
Protein RMD5 homolog B
Gene namesi
Name:RMND5B
ORF Names:UNQ2508/PRO5996
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000145916.18
HGNCiHGNC:26181 RMND5B
neXtProtiNX_Q96G75

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi64777
OpenTargetsiENSG00000145916
PharmGKBiPA144596389

Polymorphism and mutation databases

DMDMi29840869

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657091 – 393E3 ubiquitin-protein transferase RMND5BAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ96G75
MaxQBiQ96G75
PaxDbiQ96G75
PeptideAtlasiQ96G75
PRIDEiQ96G75
ProteomicsDBi76601
76602 [Q96G75-2]

PTM databases

iPTMnetiQ96G75
PhosphoSitePlusiQ96G75

Expressioni

Gene expression databases

BgeeiENSG00000145916 Expressed in 217 organ(s), highest expression level in testis
CleanExiHS_RMND5B
ExpressionAtlasiQ96G75 baseline and differential
GenevisibleiQ96G75 HS

Organism-specific databases

HPAiHPA047778

Interactioni

Subunit structurei

Identified in the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5 (PubMed:29911972). Within this complex, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, WDR26, and RANBP9 and/or RANBP10 form the catalytic core, while GID4, MKLN1, ARMC8 and YPEL5 have ancillary roles (PubMed:29911972).1 Publication

Protein-protein interaction databases

BioGridi122287, 36 interactors
IntActiQ96G75, 20 interactors
MINTiQ96G75
STRINGi9606.ENSP00000320623

Structurei

3D structure databases

ProteinModelPortaliQ96G75
SMRiQ96G75
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 148LisHPROSITE-ProRule annotationAdd BLAST33
Domaini155 – 212CTLHPROSITE-ProRule annotationAdd BLAST58

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri338 – 379RING-Gid-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2817 Eukaryota
COG5109 LUCA
GeneTreeiENSGT00720000108814
HOGENOMiHOG000211399
HOVERGENiHBG054339
InParanoidiQ96G75
OMAiCQESGVV
OrthoDBiEOG091G0OLL
PhylomeDBiQ96G75
TreeFamiTF315176

Family and domain databases

CDDicd16795 dRING_RMD5B, 1 hit
InterProiView protein in InterPro
IPR013144 CRA_dom
IPR024964 CTLH/CRA
IPR006595 CTLH_C
IPR006594 LisH
IPR037681 RMD5B_dRING
PfamiView protein in Pfam
PF10607 CLTH, 1 hit
SMARTiView protein in SMART
SM00757 CRA, 1 hit
SM00668 CTLH, 1 hit
SM00667 LisH, 1 hit
PROSITEiView protein in PROSITE
PS50897 CTLH, 1 hit
PS50896 LISH, 1 hit
PS51867 ZF_RING_GID, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96G75-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEQCACVERE LDKVLQKFLT YGQHCERSLE ELLHYVGQLR AELASAALQG
60 70 80 90 100
TPLSATLSLV MSQCCRKIKD TVQKLASDHK DIHSSVSRVG KAIDRNFDSE
110 120 130 140 150
ICGVVSDAVW DAREQQQQIL QMAIVEHLYQ QGMLSVAEEL CQESTLNVDL
160 170 180 190 200
DFKQPFLELN RILEALHEQD LGPALEWAVS HRQRLLELNS SLEFKLHRLH
210 220 230 240 250
FIRLLAGGPA KQLEALSYAR HFQPFARLHQ REIQVMMGSL VYLRLGLEKS
260 270 280 290 300
PYCHLLDSSH WAEICETFTR DACSLLGLSV ESPLSVSFAS GCVALPVLMN
310 320 330 340 350
IKAVIEQRQC TGVWNHKDEL PIEIELGMKC WYHSVFACPI LRQQTSDSNP
360 370 380 390
PIKLICGHVI SRDALNKLIN GGKLKCPYCP MEQNPADGKR IIF
Length:393
Mass (Da):44,414
Last modified:December 1, 2001 - v1
Checksum:i8C0C3E67996A5502
GO
Isoform 2 (identifier: Q96G75-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.

Note: No experimental confirmation available.
Show »
Length:333
Mass (Da):37,741
Checksum:iC0E1C3D61E8DD1F2
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5H6G4F5H6G4_HUMAN
E3 ubiquitin-protein transferase RM...
RMND5B
380Annotation score:
D6RIF9D6RIF9_HUMAN
E3 ubiquitin-protein transferase RM...
RMND5B
148Annotation score:
D6RER3D6RER3_HUMAN
E3 ubiquitin-protein transferase RM...
RMND5B
114Annotation score:
D6RFE6D6RFE6_HUMAN
E3 ubiquitin-protein transferase RM...
RMND5B
77Annotation score:
D6R9A2D6R9A2_HUMAN
E3 ubiquitin-protein transferase RM...
RMND5B
62Annotation score:
D6RFK1D6RFK1_HUMAN
E3 ubiquitin-protein transferase RM...
RMND5B
56Annotation score:

Sequence cautioni

The sequence BAB15303 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0131741 – 60Missing in isoform 2. 1 PublicationAdd BLAST60

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ494789 mRNA Translation: ABF50942.1
AY359092 mRNA Translation: AAQ89450.1
AK025971 mRNA Translation: BAB15303.1 Different initiation.
AK125337 mRNA Translation: BAG54186.1
AL713670 mRNA Translation: CAD28476.1
CH471165 Genomic DNA Translation: EAW53849.1
CH471165 Genomic DNA Translation: EAW53850.1
BC009911 mRNA Translation: AAH09911.1
CCDSiCCDS4431.1 [Q96G75-1]
RefSeqiNP_001275723.1, NM_001288794.1 [Q96G75-1]
NP_001275724.1, NM_001288795.1
NP_073599.2, NM_022762.4 [Q96G75-1]
XP_005266026.1, XM_005265969.3 [Q96G75-1]
XP_005266028.1, XM_005265971.4 [Q96G75-2]
XP_005266029.1, XM_005265972.3 [Q96G75-2]
XP_016865216.1, XM_017009727.1
XP_016865217.1, XM_017009728.1 [Q96G75-2]
UniGeneiHs.27222

Genome annotation databases

EnsembliENST00000313386; ENSP00000320623; ENSG00000145916 [Q96G75-1]
ENST00000515098; ENSP00000420875; ENSG00000145916 [Q96G75-1]
GeneIDi64777
KEGGihsa:64777
UCSCiuc003mim.5 human [Q96G75-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ494789 mRNA Translation: ABF50942.1
AY359092 mRNA Translation: AAQ89450.1
AK025971 mRNA Translation: BAB15303.1 Different initiation.
AK125337 mRNA Translation: BAG54186.1
AL713670 mRNA Translation: CAD28476.1
CH471165 Genomic DNA Translation: EAW53849.1
CH471165 Genomic DNA Translation: EAW53850.1
BC009911 mRNA Translation: AAH09911.1
CCDSiCCDS4431.1 [Q96G75-1]
RefSeqiNP_001275723.1, NM_001288794.1 [Q96G75-1]
NP_001275724.1, NM_001288795.1
NP_073599.2, NM_022762.4 [Q96G75-1]
XP_005266026.1, XM_005265969.3 [Q96G75-1]
XP_005266028.1, XM_005265971.4 [Q96G75-2]
XP_005266029.1, XM_005265972.3 [Q96G75-2]
XP_016865216.1, XM_017009727.1
XP_016865217.1, XM_017009728.1 [Q96G75-2]
UniGeneiHs.27222

3D structure databases

ProteinModelPortaliQ96G75
SMRiQ96G75
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122287, 36 interactors
IntActiQ96G75, 20 interactors
MINTiQ96G75
STRINGi9606.ENSP00000320623

PTM databases

iPTMnetiQ96G75
PhosphoSitePlusiQ96G75

Polymorphism and mutation databases

DMDMi29840869

Proteomic databases

EPDiQ96G75
MaxQBiQ96G75
PaxDbiQ96G75
PeptideAtlasiQ96G75
PRIDEiQ96G75
ProteomicsDBi76601
76602 [Q96G75-2]

Protocols and materials databases

DNASUi64777
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313386; ENSP00000320623; ENSG00000145916 [Q96G75-1]
ENST00000515098; ENSP00000420875; ENSG00000145916 [Q96G75-1]
GeneIDi64777
KEGGihsa:64777
UCSCiuc003mim.5 human [Q96G75-1]

Organism-specific databases

CTDi64777
DisGeNETi64777
EuPathDBiHostDB:ENSG00000145916.18
GeneCardsiRMND5B
HGNCiHGNC:26181 RMND5B
HPAiHPA047778
neXtProtiNX_Q96G75
OpenTargetsiENSG00000145916
PharmGKBiPA144596389
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2817 Eukaryota
COG5109 LUCA
GeneTreeiENSGT00720000108814
HOGENOMiHOG000211399
HOVERGENiHBG054339
InParanoidiQ96G75
OMAiCQESGVV
OrthoDBiEOG091G0OLL
PhylomeDBiQ96G75
TreeFamiTF315176

Miscellaneous databases

ChiTaRSiRMND5B human
GeneWikiiRMND5B
GenomeRNAii64777
PROiPR:Q96G75

Gene expression databases

BgeeiENSG00000145916 Expressed in 217 organ(s), highest expression level in testis
CleanExiHS_RMND5B
ExpressionAtlasiQ96G75 baseline and differential
GenevisibleiQ96G75 HS

Family and domain databases

CDDicd16795 dRING_RMD5B, 1 hit
InterProiView protein in InterPro
IPR013144 CRA_dom
IPR024964 CTLH/CRA
IPR006595 CTLH_C
IPR006594 LisH
IPR037681 RMD5B_dRING
PfamiView protein in Pfam
PF10607 CLTH, 1 hit
SMARTiView protein in SMART
SM00757 CRA, 1 hit
SM00668 CTLH, 1 hit
SM00667 LisH, 1 hit
PROSITEiView protein in PROSITE
PS50897 CTLH, 1 hit
PS50896 LISH, 1 hit
PS51867 ZF_RING_GID, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRMD5B_HUMAN
AccessioniPrimary (citable) accession number: Q96G75
Secondary accession number(s): Q1HE27, Q6UVY7, Q9H6F6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: December 1, 2001
Last modified: October 10, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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