UniProtKB - Q96FX7 (TRM61_HUMAN)
tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
TRMT61A
Functioni
Catalytic subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA (PubMed:16043508).
Catalytic subunit of mRNA N1-methyltransferase complex, which mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries (PubMed:29107537, PubMed:29072297).
3 PublicationsCatalytic activityi
- adenosine58 in tRNA + S-adenosyl-L-methionine = H+ + N1-methyladenosine58 in tRNA + S-adenosyl-L-homocysteinePROSITE-ProRule annotation1 PublicationEC:2.1.1.220PROSITE-ProRule annotation1 Publication
- adenosine in mRNA + S-adenosyl-L-methionine = H+ + N1-methyladenosine in mRNA + S-adenosyl-L-homocysteine2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 87 | S-adenosyl-L-methionine; via amide nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 135 | S-adenosyl-L-methioninePROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 140 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 181 | S-adenosyl-L-methioninePROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 278 | SubstrateCombined sources1 Publication | 1 |
GO - Molecular functioni
- mRNA (adenine-N1-)-methyltransferase activity Source: UniProtKB
- tRNA (adenine-N1-)-methyltransferase activity Source: InterPro
GO - Biological processi
- mRNA methylation Source: UniProtKB
- tRNA methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.220, 2681 |
PathwayCommonsi | Q96FX7 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
SignaLinki | Q96FX7 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A (EC:2.1.1.2201 Publication)Alternative name(s): mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A1 Publication (EC:2.1.1.-2 Publications) tRNA(m1A58)-methyltransferase subunit TRMT61A Short name: tRNA(m1A58)MTase subunit TRMT61A |
Gene namesi | Name:TRMT61A Synonyms:C14orf172, TRM61 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:23790, TRMT61A |
neXtProti | NX_Q96FX7 |
VEuPathDBi | HostDB:ENSG00000166166 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Other locations
- tRNA (m1A) methyltransferase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 115708 |
OpenTargetsi | ENSG00000166166 |
PharmGKBi | PA164726783 |
Miscellaneous databases
Pharosi | Q96FX7, Tbio |
Genetic variation databases
BioMutai | TRMT61A |
DMDMi | 74760827 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000233094 | 2 – 289 | tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61AAdd BLAST | 288 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 263 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q96FX7 |
jPOSTi | Q96FX7 |
MassIVEi | Q96FX7 |
MaxQBi | Q96FX7 |
PaxDbi | Q96FX7 |
PeptideAtlasi | Q96FX7 |
PRIDEi | Q96FX7 |
ProteomicsDBi | 76570 |
PTM databases
GlyGeni | Q96FX7, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96FX7 |
PhosphoSitePlusi | Q96FX7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000166166, Expressed in epithelium of bronchus and 244 other tissues |
ExpressionAtlasi | Q96FX7, baseline and differential |
Genevisiblei | Q96FX7, HS |
Organism-specific databases
HPAi | ENSG00000166166, Low tissue specificity |
Interactioni
Subunit structurei
Heterotetramer; composed of two copies of TRMT6 and two copies of TRMT61A.
1 Publication1 PublicationBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 125450, 49 interactors |
IntActi | Q96FX7, 19 interactors |
MINTi | Q96FX7 |
STRINGi | 9606.ENSP00000374399 |
Miscellaneous databases
RNActi | Q96FX7, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q96FX7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 20 – 22 | Substrate bindingCombined sources1 Publication | 3 | |
Regioni | 35 – 42 | Substrate bindingCombined sources1 Publication | 8 | |
Regioni | 64 – 65 | Substrate bindingCombined sources1 Publication | 2 | |
Regioni | 85 – 89 | Substrate bindingCombined sources1 Publication | 5 | |
Regioni | 110 – 117 | Substrate bindingCombined sources1 Publication | 8 | |
Regioni | 114 – 116 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 3 | |
Regioni | 163 – 164 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 2 | |
Regioni | 180 – 183 | Substrate bindingCombined sources1 Publication | 4 | |
Regioni | 205 – 212 | Substrate bindingCombined sources1 Publication | 8 | |
Regioni | 245 – 272 | DisorderedSequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2915, Eukaryota |
GeneTreei | ENSGT00940000154239 |
HOGENOMi | CLU_025402_4_1_1 |
InParanoidi | Q96FX7 |
OMAi | VVYPKDA |
OrthoDBi | 1032689at2759 |
PhylomeDBi | Q96FX7 |
TreeFami | TF315053 |
Family and domain databases
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR014816, tRNA_MeTrfase_Gcd14 |
PANTHERi | PTHR12133, PTHR12133, 1 hit |
Pfami | View protein in Pfam PF08704, GCD14, 1 hit |
PIRSFi | PIRSF017269, GCD14, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51620, SAM_TRM61, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSFVAYEELI KEGDTAILSL GHGAMVAVRV QRGAQTQTRH GVLRHSVDLI
60 70 80 90 100
GRPFGSKVTC GRGGWVYVLH PTPELWTLNL PHRTQILYST DIALITMMLE
110 120 130 140 150
LRPGSVVCES GTGSGSVSHA IIRTIAPTGH LHTVEFHQQR AEKAREEFQE
160 170 180 190 200
HRVGRWVTVR TQDVCRSGFG VSHVADAVFL DIPSPWEAVG HAWDALKVEG
210 220 230 240 250
GRFCSFSPCI EQVQRTCQAL AARGFSELST LEVLPQVYNV RTVSLPPPDL
260 270 280
GTGTDGPAGS DTSPFRSGTP MKEAVGHTGY LTFATKTPG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0Y2Q1 | H0Y2Q1_HUMAN | tRNA (adenine(58)-N(1))-methyltrans... | TRMT61A | 191 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_026053 | 66 | V → A1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL133367 Genomic DNA No translation available. AL139300 Genomic DNA No translation available. BC010167 mRNA Translation: AAH10167.1 AK097771 mRNA Translation: BAC05168.1 Sequence problems. |
CCDSi | CCDS41994.1 |
RefSeqi | NP_689520.2, NM_152307.2 |
Genome annotation databases
Ensembli | ENST00000389749; ENSP00000374399; ENSG00000166166 |
GeneIDi | 115708 |
KEGGi | hsa:115708 |
MANE-Selecti | ENST00000389749.9; ENSP00000374399.4; NM_152307.3; NP_689520.2 |
UCSCi | uc001yng.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL133367 Genomic DNA No translation available. AL139300 Genomic DNA No translation available. BC010167 mRNA Translation: AAH10167.1 AK097771 mRNA Translation: BAC05168.1 Sequence problems. |
CCDSi | CCDS41994.1 |
RefSeqi | NP_689520.2, NM_152307.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5CCB | X-ray | 2.00 | A | 1-289 | [»] | |
5CCX | X-ray | 2.10 | A | 1-289 | [»] | |
5CD1 | X-ray | 3.60 | A/D | 1-289 | [»] | |
SMRi | Q96FX7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 125450, 49 interactors |
IntActi | Q96FX7, 19 interactors |
MINTi | Q96FX7 |
STRINGi | 9606.ENSP00000374399 |
PTM databases
GlyGeni | Q96FX7, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96FX7 |
PhosphoSitePlusi | Q96FX7 |
Genetic variation databases
BioMutai | TRMT61A |
DMDMi | 74760827 |
Proteomic databases
EPDi | Q96FX7 |
jPOSTi | Q96FX7 |
MassIVEi | Q96FX7 |
MaxQBi | Q96FX7 |
PaxDbi | Q96FX7 |
PeptideAtlasi | Q96FX7 |
PRIDEi | Q96FX7 |
ProteomicsDBi | 76570 |
Protocols and materials databases
Antibodypediai | 6565, 73 antibodies from 20 providers |
DNASUi | 115708 |
Genome annotation databases
Ensembli | ENST00000389749; ENSP00000374399; ENSG00000166166 |
GeneIDi | 115708 |
KEGGi | hsa:115708 |
MANE-Selecti | ENST00000389749.9; ENSP00000374399.4; NM_152307.3; NP_689520.2 |
UCSCi | uc001yng.4, human |
Organism-specific databases
CTDi | 115708 |
DisGeNETi | 115708 |
GeneCardsi | TRMT61A |
HGNCi | HGNC:23790, TRMT61A |
HPAi | ENSG00000166166, Low tissue specificity |
neXtProti | NX_Q96FX7 |
OpenTargetsi | ENSG00000166166 |
PharmGKBi | PA164726783 |
VEuPathDBi | HostDB:ENSG00000166166 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2915, Eukaryota |
GeneTreei | ENSGT00940000154239 |
HOGENOMi | CLU_025402_4_1_1 |
InParanoidi | Q96FX7 |
OMAi | VVYPKDA |
OrthoDBi | 1032689at2759 |
PhylomeDBi | Q96FX7 |
TreeFami | TF315053 |
Enzyme and pathway databases
BRENDAi | 2.1.1.220, 2681 |
PathwayCommonsi | Q96FX7 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
SignaLinki | Q96FX7 |
Miscellaneous databases
BioGRID-ORCSi | 115708, 252 hits in 1049 CRISPR screens |
ChiTaRSi | TRMT61A, human |
GenomeRNAii | 115708 |
Pharosi | Q96FX7, Tbio |
PROi | PR:Q96FX7 |
RNActi | Q96FX7, protein |
Gene expression databases
Bgeei | ENSG00000166166, Expressed in epithelium of bronchus and 244 other tissues |
ExpressionAtlasi | Q96FX7, baseline and differential |
Genevisiblei | Q96FX7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR014816, tRNA_MeTrfase_Gcd14 |
PANTHERi | PTHR12133, PTHR12133, 1 hit |
Pfami | View protein in Pfam PF08704, GCD14, 1 hit |
PIRSFi | PIRSF017269, GCD14, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51620, SAM_TRM61, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRM61_HUMAN | |
Accessioni | Q96FX7Primary (citable) accession number: Q96FX7 Secondary accession number(s): A6NN78, Q8N7Q9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2006 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families