UniProtKB - Q96FX2 (DPH3_HUMAN)
Protein
DPH3 homolog
Gene
DPH3
Organism
Homo sapiens (Human)
Status
Functioni
Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in elongation factor 2 (EEF2) and which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta).By similarity
Down-regulation increases extracellular release of proteoglycans, indicating a possible role in the secretion process. Stimulates binding of GNEFR to SEC5.2 Publications
: peptidyl-diphthamide biosynthesis Pathwayi
This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 26 | Zinc or ironPROSITE-ProRule annotation | 1 | |
Metal bindingi | 28 | Zinc or ironPROSITE-ProRule annotation | 1 | |
Metal bindingi | 48 | Zinc or ironPROSITE-ProRule annotation | 1 | |
Metal bindingi | 51 | Zinc or ironPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- negative regulation of protein secretion Source: UniProtKB
- peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: UniProtKB
- positive regulation of binding Source: UniProtKB
- tRNA wobble uridine modification Source: GO_Central
Keywordsi
Ligand | Iron, Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q96FX2 |
Reactomei | R-HSA-5358493, Synthesis of diphthamide-EEF2 |
UniPathwayi | UPA00559 |
Names & Taxonomyi
Protein namesi | Recommended name: DPH3 homologAlternative name(s): CSL-type zinc finger-containing protein 2 DelGEF-interacting protein 1 Short name: DelGIP1 |
Gene namesi | Name:DPH3 Synonyms:DESR1, ZCSL2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000154813.9 |
HGNCi | HGNC:27717, DPH3 |
MIMi | 608959, gene |
neXtProti | NX_Q96FX2 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 285381 |
OpenTargetsi | ENSG00000154813 |
PharmGKBi | PA162384061 |
Miscellaneous databases
Pharosi | Q96FX2, Tdark |
Polymorphism and mutation databases
BioMutai | DPH3 |
DMDMi | 29611922 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082620 | 1 – 82 | DPH3 homologAdd BLAST | 82 |
Proteomic databases
EPDi | Q96FX2 |
jPOSTi | Q96FX2 |
MassIVEi | Q96FX2 |
MaxQBi | Q96FX2 |
PaxDbi | Q96FX2 |
PeptideAtlasi | Q96FX2 |
PRIDEi | Q96FX2 |
ProteomicsDBi | 76568 [Q96FX2-1] 76569 [Q96FX2-2] |
PTM databases
PhosphoSitePlusi | Q96FX2 |
Expressioni
Tissue specificityi
Widely expressed with highest levels in small intestine, spleen, thymus, heart, liver and lung.2 Publications
Gene expression databases
Bgeei | ENSG00000154813, Expressed in quadriceps femoris and 215 other tissues |
ExpressionAtlasi | Q96FX2, baseline and differential |
Genevisiblei | Q96FX2, HS |
Organism-specific databases
HPAi | ENSG00000154813, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsQ96FX2
With | #Exp. | IntAct |
---|---|---|
MEOX2 [Q6FHY5] | 3 | EBI-465363,EBI-16439278 |
PIH1D2 [Q8WWB5] | 6 | EBI-465363,EBI-10232538 |
SERGEF [Q9UGK8] | 7 | EBI-465363,EBI-465368 |
ZNF438 [Q7Z4V0] | 3 | EBI-465363,EBI-11962468 |
Protein-protein interaction databases
BioGRIDi | 130097, 10 interactors |
IntActi | Q96FX2, 9 interactors |
STRINGi | 9606.ENSP00000419599 |
Miscellaneous databases
RNActi | Q96FX2, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q96FX2 |
SMRi | Q96FX2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96FX2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 60 | DPH-type MBPROSITE-ProRule annotationAdd BLAST | 57 |
Domaini
The DPH-type metal-binding (MB) domain can bind either zinc or iron ions.PROSITE-ProRule annotation
Sequence similaritiesi
Belongs to the DPH3 family.Curated
Phylogenomic databases
eggNOGi | KOG2923, Eukaryota |
GeneTreei | ENSGT00390000007225 |
HOGENOMi | CLU_155991_3_0_1 |
InParanoidi | Q96FX2 |
OMAi | SLMVRVI |
PhylomeDBi | Q96FX2 |
TreeFami | TF315102 |
Family and domain databases
Gene3Di | 3.10.660.10, 1 hit |
InterProi | View protein in InterPro IPR007872, DHP_MB_dom IPR036671, DHP_MB_sf |
Pfami | View protein in Pfam PF05207, zf-CSL, 1 hit |
SUPFAMi | SSF144217, SSF144217, 1 hit |
PROSITEi | View protein in PROSITE PS51074, DPH_MB, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q96FX2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAVFHDEVEI EDFQYDEDSE TYFYPCPCGD NFSITKEDLE NGEDVATCPS
60 70 80
CSLIIKVIYD KDQFVCGETV PAPSANKELV KC
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012411 | 36 – 60 | Missing in isoform 2. 1 PublicationAdd BLAST | 25 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK022970 mRNA No translation available. BC010181 mRNA Translation: AAH10181.1 |
CCDSi | CCDS2629.1 [Q96FX2-1] CCDS43058.1 [Q96FX2-2] |
RefSeqi | NP_001040899.1, NM_001047434.2 [Q96FX2-2] NP_996662.1, NM_206831.2 [Q96FX2-1] |
Genome annotation databases
Ensembli | ENST00000383775; ENSP00000373285; ENSG00000154813 [Q96FX2-2] ENST00000488423; ENSP00000419599; ENSG00000154813 [Q96FX2-1] |
GeneIDi | 285381 |
KEGGi | hsa:285381 |
UCSCi | uc003cau.4, human [Q96FX2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK022970 mRNA No translation available. BC010181 mRNA Translation: AAH10181.1 |
CCDSi | CCDS2629.1 [Q96FX2-1] CCDS43058.1 [Q96FX2-2] |
RefSeqi | NP_001040899.1, NM_001047434.2 [Q96FX2-2] NP_996662.1, NM_206831.2 [Q96FX2-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JR7 | NMR | - | A | 1-81 | [»] | |
BMRBi | Q96FX2 | |||||
SMRi | Q96FX2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 130097, 10 interactors |
IntActi | Q96FX2, 9 interactors |
STRINGi | 9606.ENSP00000419599 |
PTM databases
PhosphoSitePlusi | Q96FX2 |
Polymorphism and mutation databases
BioMutai | DPH3 |
DMDMi | 29611922 |
Proteomic databases
EPDi | Q96FX2 |
jPOSTi | Q96FX2 |
MassIVEi | Q96FX2 |
MaxQBi | Q96FX2 |
PaxDbi | Q96FX2 |
PeptideAtlasi | Q96FX2 |
PRIDEi | Q96FX2 |
ProteomicsDBi | 76568 [Q96FX2-1] 76569 [Q96FX2-2] |
Protocols and materials databases
Antibodypediai | 26896, 36 antibodies |
DNASUi | 285381 |
Genome annotation databases
Ensembli | ENST00000383775; ENSP00000373285; ENSG00000154813 [Q96FX2-2] ENST00000488423; ENSP00000419599; ENSG00000154813 [Q96FX2-1] |
GeneIDi | 285381 |
KEGGi | hsa:285381 |
UCSCi | uc003cau.4, human [Q96FX2-1] |
Organism-specific databases
CTDi | 285381 |
DisGeNETi | 285381 |
EuPathDBi | HostDB:ENSG00000154813.9 |
GeneCardsi | DPH3 |
HGNCi | HGNC:27717, DPH3 |
HPAi | ENSG00000154813, Low tissue specificity |
MIMi | 608959, gene |
neXtProti | NX_Q96FX2 |
OpenTargetsi | ENSG00000154813 |
PharmGKBi | PA162384061 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2923, Eukaryota |
GeneTreei | ENSGT00390000007225 |
HOGENOMi | CLU_155991_3_0_1 |
InParanoidi | Q96FX2 |
OMAi | SLMVRVI |
PhylomeDBi | Q96FX2 |
TreeFami | TF315102 |
Enzyme and pathway databases
UniPathwayi | UPA00559 |
PathwayCommonsi | Q96FX2 |
Reactomei | R-HSA-5358493, Synthesis of diphthamide-EEF2 |
Miscellaneous databases
BioGRID-ORCSi | 285381, 655 hits in 834 CRISPR screens |
ChiTaRSi | DPH3, human |
EvolutionaryTracei | Q96FX2 |
GenomeRNAii | 285381 |
Pharosi | Q96FX2, Tdark |
PROi | PR:Q96FX2 |
RNActi | Q96FX2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000154813, Expressed in quadriceps femoris and 215 other tissues |
ExpressionAtlasi | Q96FX2, baseline and differential |
Genevisiblei | Q96FX2, HS |
Family and domain databases
Gene3Di | 3.10.660.10, 1 hit |
InterProi | View protein in InterPro IPR007872, DHP_MB_dom IPR036671, DHP_MB_sf |
Pfami | View protein in Pfam PF05207, zf-CSL, 1 hit |
SUPFAMi | SSF144217, SSF144217, 1 hit |
PROSITEi | View protein in PROSITE PS51074, DPH_MB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPH3_HUMAN | |
Accessioni | Q96FX2Primary (citable) accession number: Q96FX2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 4, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - UniProtKB entry view manual
User manual for the UniProtKB entry view