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UniProtKB - Q96FW1 (OTUB1_HUMAN)

Entry version 179 (10 Feb 2021)
Sequence version 2 (01 Mar 2004)
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Protein

Ubiquitin thioesterase OTUB1

Gene

OTUB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.
Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain.

Miscellaneous

In the structure described by PubMed:18954305, the His-265 active site of the catalytic triad is located too far to interact directly with the active site Cys-91. A possible explanation is that OTUB1 is in inactive conformation in absence of ubiquitin and a conformation change may move His-265 in the proximity of Cys-91 in presence of ubiquitin substrate.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

By free ubiquitin: binding of free ubiquitin triggers conformational changes in the OTU domain and formation of a ubiquitin-binding helix in the N-terminus, promoting binding of the conjugated donor ubiquitin in UBE2N/UBC13 to OTUB1.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei23Required for proximal ubiquitin-binding1 Publication1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei88Combined sources1 Publication1
Active sitei91Nucleophile1 Publication1 Publication1
Sitei221Interacts with free ubiquitin1 Publication1
Sitei235Interacts with free ubiquitin1 Publication1
Sitei237Interacts with free ubiquitin1 Publication1
Sitei261Interacts with free ubiquitin1 Publication1
Active sitei2651 Publication1 Publication1
Sitei266Interacts with free ubiquitinCombined sources2 Publications1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processAdaptive immunity, DNA damage, DNA repair, Immunity, Ubl conjugation pathway

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q96FW1

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-5689880, Ub-specific processing proteases
R-HSA-5689896, Ovarian tumor domain proteases

SIGNOR Signaling Network Open Resource

More...SIGNORi		Q96FW1

Protein family/group databases

MEROPS protease database

More...MEROPSi		C65.001

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ubiquitin thioesterase OTUB1 (EC:3.4.19.121 Publication)
Alternative name(s):
Deubiquitinating enzyme OTUB1
OTU domain-containing ubiquitin aldehyde-binding protein 1
Otubain-1
Short name:
hOTU1
Ubiquitin-specific-processing protease OTUB1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:OTUB1
Synonyms:OTB1, OTU1
ORF Names:HSPC263
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 11

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:23077, OTUB1

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		608337, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q96FW1

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000167770.11

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi23C → A: Abolishes only ubiquitin-vinylsulfone adduct formation. 1 Publication1
Mutagenesisi33Q → R: Impairs inhibition of UBE2N/UBC13. 1 Publication1
Mutagenesisi37I → T: Impairs inhibition of UBE2N/UBC13. 1 Publication1
Mutagenesisi39Q → L: Does not affect activity in DNA repair and ability to inhibit UBE2N/UBC13. 1 Publication1
Mutagenesisi87P → G: Slightly improves ability to cleave 'K63'-linked ubiquitin. 1 Publication1
Mutagenesisi88D → E: Abolishes hydrolase activity in vitro. Abolishes ability to inhibit RNF168; when associated with S-91 and A-265. 2 Publications1
Mutagenesisi91C → A: Prevents RNF128 autoubiquitination, and stabilizes RNF128 in vivo. Abolishes both ubiquitin-binding and adduct formation with ubiquitin-vinylsulfone. 7 Publications1
Mutagenesisi91C → S: Abolishes hydrolase activity in vitro. Does not affect ability to inhibit RNF168. Abolishes ability to inhibit RNF168; when associated with A-88 and A-265. 7 Publications1
Mutagenesisi116A → T: Does not affect ability to inhibit UBE2N/UBC13. 1 Publication1
Mutagenesisi134T → R: Impairs inhibition of UBE2N/UBC13. 1 Publication1
Mutagenesisi137D → G: Impairs inhibition of UBE2N/UBC13. 1 Publication1
Mutagenesisi176R → L: No effect on RNF128. 1 Publication1
Mutagenesisi190F → S: Fails to inhibit ubiquitin conjugation by UBE2N/UBC13. 1 Publication1
Mutagenesisi212C → A: No effect on RNF128. 2 Publications1
Mutagenesisi261Y → H: Impairs inhibition of UBE2N/UBC13. 1 Publication1
Mutagenesisi263P → L: Fails to inhibit ubiquitin conjugation by UBE2N/UBC13. 1 Publication1
Mutagenesisi265H → A: Abolishes ability to inhibit RNF168; when associated with A-88 and S-91. 2 Publications1
Mutagenesisi265H → R: Abolishes hydrolase activity in vitro. 2 Publications1

Organism-specific databases

DisGeNET

More...DisGeNETi		55611

Open Targets

More...OpenTargetsi		ENSG00000167770

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA134988141

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q96FW1, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		OTUB1

Domain mapping of disease mutations (DMDM)

More...DMDMi		44888286

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002210082 – 271Ubiquitin thioesterase OTUB1Add BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylalanineCombined sources1
Modified residuei16PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

The CPTAC Assay portal

More...CPTACi		CPTAC-244
CPTAC-245

Encyclopedia of Proteome Dynamics

More...EPDi		Q96FW1

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q96FW1

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q96FW1

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q96FW1

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q96FW1

PeptideAtlas

More...PeptideAtlasi		Q96FW1

PRoteomics IDEntifications database

More...PRIDEi		Q96FW1

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		76566 [Q96FW1-1]
76567 [Q96FW1-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q96FW1

MetOSite database of methionine sulfoxide sites

More...MetOSitei		Q96FW1

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q96FW1

SwissPalm database of S-palmitoylation events

More...SwissPalmi		Q96FW1

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells.2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000167770, Expressed in anterior cingulate cortex and 246 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q96FW1, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q96FW1, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000167770, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Isoform 1 and isoform 2 interact with RNF128. Isoform 1 forms a ternary complex with RNF128 and USP8.

Isoform 1 interacts with the C-terminal UCH catalytic domain of USP8. Isoform 2 does not associate with USP8.

Interacts with FUS, ESR1 and RACK1.

Interacts with UBE2N/UBC13.

6 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Q96FW1
With#Exp.IntAct
A2M [P01023]3EBI-1058491,EBI-640741
APP [P05067]3EBI-1058491,EBI-77613
ATXN1 [P54253]6EBI-1058491,EBI-930964
BAG6 - isoform 2 [P46379-2]3EBI-1058491,EBI-10988864
BIRC2 [Q13490]3EBI-1058491,EBI-514538
BRK1 [Q8WUW1]3EBI-1058491,EBI-2837444
DCTN1 - isoform 5 [Q14203-5]3EBI-1058491,EBI-25840379
DMWD [G5E9A7]3EBI-1058491,EBI-10976677
DNAJB6 - isoform B [O75190-2]3EBI-1058491,EBI-12593112
DNALI1 [O14645]3EBI-1058491,EBI-395638
DNM2 - isoform 2 [P50570-2]3EBI-1058491,EBI-10968534
FUS [P35637]3EBI-1058491,EBI-400434
GFAP [P14136]3EBI-1058491,EBI-744302
GLE1 [Q53GS7]3EBI-1058491,EBI-1955541
GRN [P28799]3EBI-1058491,EBI-747754
HEXB [P07686]3EBI-1058491,EBI-7133736
HIF1AN [Q9NWT6]5EBI-1058491,EBI-745632
HSPB1 [P04792]3EBI-1058491,EBI-352682
HTRA2 [O43464]3EBI-1058491,EBI-517086
ICAM5 [Q9UMF0]3EBI-1058491,EBI-6398041
JPH3 [Q8WXH2]3EBI-1058491,EBI-1055254
KIF1B - isoform 2 [O60333-2]3EBI-1058491,EBI-10975473
KLF11 [O14901]3EBI-1058491,EBI-948266
MDM2 [Q00987]5EBI-1058491,EBI-389668
MECP2 [P51608]3EBI-1058491,EBI-1189067
NDUFV2 [P19404]3EBI-1058491,EBI-713665
NF2 - isoform 4 [P35240-4]3EBI-1058491,EBI-1014514
PARK7 [Q99497]4EBI-1058491,EBI-1164361
PRKN - isoform 5 [O60260-5]3EBI-1058491,EBI-21251460
PRPS1 [P60891]3EBI-1058491,EBI-749195
PSEN1 - isoform 2 [P49768-2]3EBI-1058491,EBI-11047108
RNF11 [Q9Y3C5]3EBI-1058491,EBI-396669
SNCA [P37840]3EBI-1058491,EBI-985879
SOD1 [P00441]3EBI-1058491,EBI-990792
SPRED1 [Q7Z699]3EBI-1058491,EBI-5235340
TARDBP [Q13148]8EBI-1058491,EBI-372899
TP53 [P04637]8EBI-1058491,EBI-366083
TPP1 [O14773]3EBI-1058491,EBI-2800203
TSC1 [Q86WV8]3EBI-1058491,EBI-12806590
TTR [P02766]4EBI-1058491,EBI-711909
UBA1 [P22314]4EBI-1058491,EBI-709688
UBB [P0CG47]3EBI-1058491,EBI-413034
UBE2D1 [P51668]14EBI-1058491,EBI-743540
UBE2D2 [P62837]16EBI-1058491,EBI-347677
UBE2D3 [P61077]14EBI-1058491,EBI-348268
UBE2D4 [Q9Y2X8]9EBI-1058491,EBI-745527
UBE2E1 [P51965]3EBI-1058491,EBI-348546
UBE2E2 [Q96LR5]8EBI-1058491,EBI-2129763
UBE2E3 [Q969T4]8EBI-1058491,EBI-348496
VHL - isoform 2 [P40337-2]3EBI-1058491,EBI-12157263
WFS1 [O76024]3EBI-1058491,EBI-720609
ypkA [Q56921] from Yersinia enterocolitica.5EBI-1058491,EBI-8022937
ypkA [Q9RI12] from Yersinia pestis.3EBI-1058491,EBI-2849107
Isoform 1 [Q96FW1-1]
With#Exp.IntAct
UBE2N [P61088]5EBI-15972141,EBI-1052908

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		120751, 240 interactors

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		Q96FW1

Database of interacting proteins

More...DIPi		DIP-41158N

Protein interaction database and analysis system

More...IntActi		Q96FW1, 371 interactors

Molecular INTeraction database

More...MINTi		Q96FW1

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000444357

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q96FW1, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q96FW1

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		Q96FW1

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini80 – 271OTUPROSITE-ProRule annotationAdd BLAST192

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni130 – 138Ubiquitin-conjugating enzyme E2 binding1 Publication9
Regioni169 – 177Ubiquitin-conjugating enzyme E2 binding1 Publication9
Regioni189 – 195Free ubiquitin bindingCombined sources2 Publications7
Regioni206 – 213Ubiquitin-conjugating enzyme E2 bindingCombined sources2 Publications8
Regioni214 – 221Free ubiquitin bindingCombined sources2 Publications8
Regioni245 – 251Free ubiquitin bindingCombined sources2 Publications7

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

In addition to ubiquitin-binding at the Cys-91 active site, a proximal ubiquitin-binding site is also present at Cys-23 Occupancy of the active site is needed to enable tight binding to the second site. Distinct binding sites for the ubiquitins may allow to discriminate among different isopeptide linkages (i.e. 'Lys-48'-, 'Lys-63'-linked polyubiquitin) in polyubiquitin substrates and achieve linkage-specific deubiquitination.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the peptidase C65 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG3991, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00390000006979

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q96FW1

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q96FW1

TreeFam database of animal gene trees

More...TreeFami		TF314145

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.1300.20, 1 hit
3.30.200.60, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR003323, OTU_dom
IPR030298, OTUB1
IPR016615, Otubain
IPR038765, Papain-like_cys_pep_sf
IPR019400, Peptidase_C65_otubain
IPR042468, Peptidase_C65_otubain_sub1
IPR042467, Peptidase_C65_otubain_sub2

The PANTHER Classification System

More...PANTHERi		PTHR12931, PTHR12931, 1 hit
PTHR12931:SF19, PTHR12931:SF19, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF10275, Peptidase_C65, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF013503, Ubiquitin_thioesterase_Otubain, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF54001, SSF54001, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50802, OTU, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96FW1-1) [UniParc]FASTAAdd to basket
Also known as: Otubain-1

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE 
        60         70         80         90        100
RLELSVLYKE YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH 
       110        120        130        140        150
LEALLDDSKE LQRFKAVSAK SKEDLVSQGF TEFTIEDFHN TFMDLIEQVE 
       160        170        180        190        200
KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV 
       210        220        230        240        250
KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHIFP 
       260        270 
EGSEPKVYLL YRPGHYDILY K
Length:271
Mass (Da):31,284
Last modified:March 1, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i63188EE1DC5FD66F
GO
Isoform 2 (identifier: Q96FW1-2) [UniParc]FASTAAdd to basket
Also known as: ARF-1

The sequence of this isoform differs from the canonical sequence as follows:
     1-112: MAAEEPQQQK...ALLDDSKELQ → MMKPSWLSRT...MLGPPFHPTP

Note: Lacks the catalytic sites for protease activity.Curated
Show »
Length:315
Mass (Da):35,285
Checksum:i3B37EB8F1B3B2404
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KR44J3KR44_HUMAN
Ubiquitin thioesterase
OTUB1
272Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F5H6Q1F5H6Q1_HUMAN
Ubiquitin thioesterase
OTUB1
280Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F5GYN4F5GYN4_HUMAN
Ubiquitinyl hydrolase 1
OTUB1
241Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F5GYJ8F5GYJ8_HUMAN
Ubiquitinyl hydrolase 1
OTUB1
286Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F5H3F0F5H3F0_HUMAN
Ubiquitinyl hydrolase 1
OTUB1
170Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F5H4F3F5H4F3_HUMAN
Ubiquitin thioesterase OTUB1
OTUB1
102Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAF28941 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti61Y → C in AAH10368 (PubMed:15489334).Curated1
Sequence conflicti151K → R in BAA90956 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0094641 – 112MAAEE…SKELQ → MMKPSWLSRTEFSKRLLCRT LWCQSGWSSRSYTRSMLKMT TSINRRSRTSTKSTRTSARP GLTATVSIGLSDSPTWRHCW MTARSCSGEKGGHWAPRQVG VYLLPGRVGCVSSRVSPSFP GDGLDSGLARRGSAVSALAS GLVEEPMLGPPFHPTP in isoform 2. 1 PublicationAdd BLAST112

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AY177200 mRNA Translation: AAO27702.1
AF161381 mRNA Translation: AAF28941.1 Different initiation.
AK000120 mRNA Translation: BAA90956.1
AP000721 Genomic DNA No translation available.
BC007519 mRNA Translation: AAH07519.1
BC010368 mRNA Translation: AAH10368.1
BC107701 mRNA Translation: AAI07702.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS8055.1 [Q96FW1-1]

NCBI Reference Sequences

More...RefSeqi		NP_060140.2, NM_017670.2 [Q96FW1-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000428192; ENSP00000402551; ENSG00000167770 [Q96FW1-1]
ENST00000538426; ENSP00000444357; ENSG00000167770 [Q96FW1-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		55611

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:55611

UCSC genome browser

More...UCSCi		uc001nyf.2, human [Q96FW1-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY177200 mRNA Translation: AAO27702.1
AF161381 mRNA Translation: AAF28941.1 Different initiation.
AK000120 mRNA Translation: BAA90956.1
AP000721 Genomic DNA No translation available.
BC007519 mRNA Translation: AAH07519.1
BC010368 mRNA Translation: AAH10368.1
BC107701 mRNA Translation: AAI07702.1
CCDSiCCDS8055.1 [Q96FW1-1]
RefSeqiNP_060140.2, NM_017670.2 [Q96FW1-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZFYX-ray1.69A40-271[»]
3VONX-ray3.15A/H/O/V/c/j45-271[»]
4DDGX-ray3.30A/B/C/J/K/L25-271[»]
4DDIX-ray3.80A/B/C25-271[»]
4DHZX-ray3.11A1-45[»]
4I6LX-ray2.49A45-271[»]
4LDTX-ray1.90A1-45[»]
SMRiQ96FW1
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi120751, 240 interactors
CORUMiQ96FW1
DIPiDIP-41158N
IntActiQ96FW1, 371 interactors
MINTiQ96FW1
STRINGi9606.ENSP00000444357

Protein family/group databases

MEROPSiC65.001

PTM databases

iPTMnetiQ96FW1
MetOSiteiQ96FW1
PhosphoSitePlusiQ96FW1
SwissPalmiQ96FW1

Genetic variation databases

BioMutaiOTUB1
DMDMi44888286

Proteomic databases

CPTACiCPTAC-244
CPTAC-245
EPDiQ96FW1
jPOSTiQ96FW1
MassIVEiQ96FW1
MaxQBiQ96FW1
PaxDbiQ96FW1
PeptideAtlasiQ96FW1
PRIDEiQ96FW1
ProteomicsDBi76566 [Q96FW1-1]
76567 [Q96FW1-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		29104, 451 antibodies

The CPTC Antibody Portal

More...CPTCi		Q96FW1, 3 antibodies

The DNASU plasmid repository

More...DNASUi		55611

Genome annotation databases

EnsembliENST00000428192; ENSP00000402551; ENSG00000167770 [Q96FW1-1]
ENST00000538426; ENSP00000444357; ENSG00000167770 [Q96FW1-1]
GeneIDi55611
KEGGihsa:55611
UCSCiuc001nyf.2, human [Q96FW1-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		55611
DisGeNETi55611

GeneCards: human genes, protein and diseases

More...GeneCardsi		OTUB1
HGNCiHGNC:23077, OTUB1
HPAiENSG00000167770, Low tissue specificity
MIMi608337, gene
neXtProtiNX_Q96FW1
OpenTargetsiENSG00000167770
PharmGKBiPA134988141
VEuPathDBiHostDB:ENSG00000167770.11

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG3991, Eukaryota
GeneTreeiENSGT00390000006979
InParanoidiQ96FW1
PhylomeDBiQ96FW1
TreeFamiTF314145

Enzyme and pathway databases

PathwayCommonsiQ96FW1
ReactomeiR-HSA-5689880, Ub-specific processing proteases
R-HSA-5689896, Ovarian tumor domain proteases
SIGNORiQ96FW1

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		55611, 27 hits in 881 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		OTUB1, human
EvolutionaryTraceiQ96FW1

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		OTUB1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		55611
PharosiQ96FW1, Tbio

Protein Ontology

More...PROi		PR:Q96FW1
RNActiQ96FW1, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000167770, Expressed in anterior cingulate cortex and 246 other tissues
ExpressionAtlasiQ96FW1, baseline and differential
GenevisibleiQ96FW1, HS

Family and domain databases

Gene3Di1.20.1300.20, 1 hit
3.30.200.60, 1 hit
InterProiView protein in InterPro
IPR003323, OTU_dom
IPR030298, OTUB1
IPR016615, Otubain
IPR038765, Papain-like_cys_pep_sf
IPR019400, Peptidase_C65_otubain
IPR042468, Peptidase_C65_otubain_sub1
IPR042467, Peptidase_C65_otubain_sub2
PANTHERiPTHR12931, PTHR12931, 1 hit
PTHR12931:SF19, PTHR12931:SF19, 1 hit
PfamiView protein in Pfam
PF10275, Peptidase_C65, 1 hit
PIRSFiPIRSF013503, Ubiquitin_thioesterase_Otubain, 1 hit
SUPFAMiSSF54001, SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50802, OTU, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiOTUB1_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q96FW1
Secondary accession number(s): Q32Q78
, Q96II3, Q9NXQ4, Q9P0B8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: February 10, 2021
This is version 179 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
  5. Peptidase families
    Classification of peptidase families and list of entries
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