UniProtKB - Q96FT7 (ASIC4_HUMAN)
Protein
Acid-sensing ion channel 4
Gene
ASIC4
Organism
Homo sapiens (Human)
Status
Functioni
Probable cation channel with high affinity for sodium. In vitro, has no proton-gated channel activity.1 Publication
GO - Molecular functioni
- ion channel activity Source: ProtInc
- ligand-gated sodium channel activity Source: GO_Central
- sodium ion transmembrane transporter activity Source: ProtInc
GO - Biological processi
- behavioral fear response Source: Ensembl
- sodium ion transmembrane transport Source: GO_Central
Keywordsi
Molecular function | Ion channel, Sodium channel |
Biological process | Ion transport, Sodium transport, Transport |
Ligand | Sodium |
Enzyme and pathway databases
PathwayCommonsi | Q96FT7 |
Reactomei | R-HSA-2672351, Stimuli-sensing channels |
Names & Taxonomyi
Protein namesi | Recommended name: Acid-sensing ion channel 4Short name: ASIC4 Alternative name(s): Amiloride-sensitive cation channel 4 Amiloride-sensitive cation channel 4, pituitary |
Gene namesi | Name:ASIC4 Synonyms:ACCN4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:21263, ASIC4 |
MIMi | 606715, gene |
neXtProti | NX_Q96FT7 |
VEuPathDBi | HostDB:ENSG00000072182.12 |
Subcellular locationi
Other locations
- Membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 128 – 195 | CytoplasmicSequence analysisAdd BLAST | 68 | |
Transmembranei | 196 – 216 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 217 – 546 | ExtracellularSequence analysisAdd BLAST | 330 | |
Transmembranei | 547 – 567 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 568 – 647 | CytoplasmicSequence analysisAdd BLAST | 80 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 549 | G → A: No effect on channel function. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 55515 |
PharmGKBi | PA134956731 |
Miscellaneous databases
Pharosi | Q96FT7, Tbio |
Genetic variation databases
BioMutai | ASIC4 |
DMDMi | 296434387 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000181304 | 1 – 647 | Acid-sensing ion channel 4Add BLAST | 647 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 245 ↔ 329 | By similarity | ||
Disulfide bondi | 307 ↔ 314 | By similarity | ||
Glycosylationi | 318 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 370 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 423 ↔ 483 | By similarity | ||
Disulfide bondi | 445 ↔ 479 | By similarity | ||
Disulfide bondi | 449 ↔ 477 | By similarity | ||
Glycosylationi | 484 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | Q96FT7 |
PaxDbi | Q96FT7 |
PeptideAtlasi | Q96FT7 |
PRIDEi | Q96FT7 |
ProteomicsDBi | 76552 [Q96FT7-1] 76553 [Q96FT7-2] 76554 [Q96FT7-4] |
PTM databases
GlyGeni | Q96FT7, 3 sites |
iPTMneti | Q96FT7 |
PhosphoSitePlusi | Q96FT7 |
Expressioni
Tissue specificityi
Expressed in pituitary gland. Weakly expressed in brain, vestibular system and organ of Corti.1 Publication
Gene expression databases
Bgeei | ENSG00000072182, Expressed in pituitary gland and 110 other tissues |
Genevisiblei | Q96FT7, HS |
Organism-specific databases
HPAi | ENSG00000072182, Tissue enhanced (brain, pituitary gland) |
Interactioni
Subunit structurei
Homotrimer or heterotrimer with other ASIC proteins.
By similarityBinary interactionsi
Hide detailsQ96FT7
With | #Exp. | IntAct |
---|---|---|
OTX2 - isoform 2 [P32243-2] | 3 | EBI-9116154,EBI-9087860 |
Isoform 2 [Q96FT7-2]
With | #Exp. | IntAct |
---|---|---|
WFS1 [O76024] | 3 | EBI-25898949,EBI-720609 |
Isoform 3 [Q96FT7-4]
Protein-protein interaction databases
BioGRIDi | 120693, 46 interactors |
IntActi | Q96FT7, 74 interactors |
STRINGi | 9606.ENSP00000326627 |
Miscellaneous databases
RNActi | Q96FT7, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC4 subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4294, Eukaryota |
HOGENOMi | CLU_020415_1_2_1 |
InParanoidi | Q96FT7 |
OMAi | AIIHEYC |
OrthoDBi | 686369at2759 |
PhylomeDBi | Q96FT7 |
TreeFami | TF330663 |
Family and domain databases
InterProi | View protein in InterPro IPR001873, ENaC IPR020903, ENaC_CS |
PANTHERi | PTHR11690, PTHR11690, 1 hit |
Pfami | View protein in Pfam PF00858, ASC, 1 hit |
PRINTSi | PR01078, AMINACHANNEL |
PROSITEi | View protein in PROSITE PS01206, ASC, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q96FT7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLSGAAGAAR RGGAALAPSL TRSLAGTHAG ADSCAGADKG SHKETIEERD
60 70 80 90 100
KRQQRQQRQR QHQGCGAAGS GSDSPTSGPH PVPVLFPLAL SLEEQPLPPL
110 120 130 140 150
PLGRAPGLLA REGQGREALA SPSSRGQMPI EIVCKIKFAE EDAKPKEKEA
160 170 180 190 200
GDEQSLLGAV APGAAPRDLA TFASTSTLHG LGRACGPGPH GLRRTLWALA
210 220 230 240 250
LLTSLAAFLY QAAGLARGYL TRPHLVAMDP AAPAPVAGFP AVTLCNINRF
260 270 280 290 300
RHSALSDADI FHLANLTGLP PKDRDGHRAA GLRYPEPDMV DILNRTGHQL
310 320 330 340 350
ADMLKSCNFS GHHCSASNFS VVYTRYGKCY TFNADPRSSL PSRAGGMGSG
360 370 380 390 400
LEIMLDIQQE EYLPIWRETN ETSFEAGIRV QIHSQEEPPY IHQLGFGVSP
410 420 430 440 450
GFQTFVSCQE QRLTYLPQPW GNCRAESELR EPELQGYSAY SVSACRLRCE
460 470 480 490 500
KEAVLQRCHC RMVHMPDSLG GGPEGPCFCP TPCNLTRYGK EISMVRIPNR
510 520 530 540 550
GSARYLARKY NRNETYIREN FLVLDVFFEA LTSEAMEQRA AYGLSALLGD
560 570 580 590 600
LGGQMGLFIG ASILTLLEIL DYIYEVSWDR LKRVWRRPKT PLRTSTGGIS
610 620 630 640
TLGLQELKEQ SPCPSRGRVE GGGVSSLLPN HHHPHGPPGG LFEDFAC
Sequence cautioni
The sequence CAB93980 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAC51338 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052038 | 614 | P → Q. Corresponds to variant dbSNP:rs6436153Ensembl. | 1 | |
Natural variantiVAR_052039 | 616 | R → L3 PublicationsCorresponds to variant dbSNP:rs11689281Ensembl. | 1 | |
Natural variantiVAR_059806 | 619 | V → A3 PublicationsCorresponds to variant dbSNP:rs11695248Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015609 | 413 – 417 | LTYLP → VSISC in isoform 2. 1 Publication | 5 | |
Alternative sequenceiVSP_015610 | 418 – 647 | Missing in isoform 2. 1 PublicationAdd BLAST | 230 | |
Alternative sequenceiVSP_015611 | 466 | P → PGNETICPPNIYIECADHTL in isoform 3. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ271643 Genomic DNA Translation: CAB93980.1 Different initiation. AC009955 Genomic DNA No translation available. AC053503 Genomic DNA Translation: AAY15054.1 AC139723 Genomic DNA No translation available. BC010439 mRNA Translation: AAH10439.1 BC031812 mRNA Translation: AAH31812.1 AJ408881 , AJ408882, AJ408883, AJ408884 Genomic DNA Translation: CAC51338.1 Different initiation. |
RefSeqi | NP_061144.3, NM_018674.5 NP_878267.2, NM_182847.2 |
Genome annotation databases
Ensembli | ENST00000347842; ENSP00000326627; ENSG00000072182 ENST00000358078; ENSP00000350786; ENSG00000072182 |
GeneIDi | 55515 |
KEGGi | hsa:55515 |
UCSCi | uc002vma.4, human [Q96FT7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ271643 Genomic DNA Translation: CAB93980.1 Different initiation. AC009955 Genomic DNA No translation available. AC053503 Genomic DNA Translation: AAY15054.1 AC139723 Genomic DNA No translation available. BC010439 mRNA Translation: AAH10439.1 BC031812 mRNA Translation: AAH31812.1 AJ408881 , AJ408882, AJ408883, AJ408884 Genomic DNA Translation: CAC51338.1 Different initiation. |
RefSeqi | NP_061144.3, NM_018674.5 NP_878267.2, NM_182847.2 |
3D structure databases
SMRi | Q96FT7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 120693, 46 interactors |
IntActi | Q96FT7, 74 interactors |
STRINGi | 9606.ENSP00000326627 |
PTM databases
GlyGeni | Q96FT7, 3 sites |
iPTMneti | Q96FT7 |
PhosphoSitePlusi | Q96FT7 |
Genetic variation databases
BioMutai | ASIC4 |
DMDMi | 296434387 |
Proteomic databases
MassIVEi | Q96FT7 |
PaxDbi | Q96FT7 |
PeptideAtlasi | Q96FT7 |
PRIDEi | Q96FT7 |
ProteomicsDBi | 76552 [Q96FT7-1] 76553 [Q96FT7-2] 76554 [Q96FT7-4] |
Protocols and materials databases
Antibodypediai | 20142, 137 antibodies |
DNASUi | 55515 |
Genome annotation databases
Ensembli | ENST00000347842; ENSP00000326627; ENSG00000072182 ENST00000358078; ENSP00000350786; ENSG00000072182 |
GeneIDi | 55515 |
KEGGi | hsa:55515 |
UCSCi | uc002vma.4, human [Q96FT7-1] |
Organism-specific databases
CTDi | 55515 |
DisGeNETi | 55515 |
GeneCardsi | ASIC4 |
HGNCi | HGNC:21263, ASIC4 |
HPAi | ENSG00000072182, Tissue enhanced (brain, pituitary gland) |
MIMi | 606715, gene |
neXtProti | NX_Q96FT7 |
PharmGKBi | PA134956731 |
VEuPathDBi | HostDB:ENSG00000072182.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4294, Eukaryota |
HOGENOMi | CLU_020415_1_2_1 |
InParanoidi | Q96FT7 |
OMAi | AIIHEYC |
OrthoDBi | 686369at2759 |
PhylomeDBi | Q96FT7 |
TreeFami | TF330663 |
Enzyme and pathway databases
PathwayCommonsi | Q96FT7 |
Reactomei | R-HSA-2672351, Stimuli-sensing channels |
Miscellaneous databases
BioGRID-ORCSi | 55515, 3 hits in 988 CRISPR screens |
ChiTaRSi | ASIC4, human |
GeneWikii | ACCN4 |
GenomeRNAii | 55515 |
Pharosi | Q96FT7, Tbio |
PROi | PR:Q96FT7 |
RNActi | Q96FT7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000072182, Expressed in pituitary gland and 110 other tissues |
Genevisiblei | Q96FT7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR001873, ENaC IPR020903, ENaC_CS |
PANTHERi | PTHR11690, PTHR11690, 1 hit |
Pfami | View protein in Pfam PF00858, ASC, 1 hit |
PRINTSi | PR01078, AMINACHANNEL |
PROSITEi | View protein in PROSITE PS01206, ASC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASIC4_HUMAN | |
Accessioni | Q96FT7Primary (citable) accession number: Q96FT7 Secondary accession number(s): Q53SB7 Q9NQA4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2005 |
Last sequence update: | May 18, 2010 | |
Last modified: | April 7, 2021 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families