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Protein

Multidrug and toxin extrusion protein 1

Gene

SLC47A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes.5 Publications

Kineticsi

  1. KM=0.38 mM for TEA2 Publications
  2. KM=0.10 mM for MPP2 Publications
  3. KM=0.17 mM for cimetidine2 Publications
  4. KM=0.78 mM for metformin2 Publications
  5. KM=2.1 mM for guanidine2 Publications
  6. KM=1.23 mM for procainamide2 Publications
  7. KM=0.07 mM for topotecan2 Publications
  8. KM=0.47 mM for estrone sulfate2 Publications
  9. KM=2.64 mM for acyclovir2 Publications
  10. KM=5.12 mM for ganciclovir2 Publications
  1. Vmax=1.185 nmol/min/mg enzyme toward TEA2 Publications
  2. Vmax=0.735 nmol/min/mg enzyme toward MPP2 Publications
  3. Vmax=0.135 nmol/min/mg enzyme toward cimetidine2 Publications
  4. Vmax=2.23 nmol/min/mg enzyme toward metformin2 Publications
  5. Vmax=0.89 nmol/min/mg enzyme toward guanidine2 Publications
  6. Vmax=3.78 nmol/min/mg enzyme toward procainamide2 Publications
  7. Vmax=0.21 nmol/min/mg enzyme toward topotecan2 Publications
  8. Vmax=0.265 nmol/min/mg enzyme toward estrone sulfate2 Publications
  9. Vmax=0.62 nmol/min/mg enzyme toward acyclovir2 Publications
  10. Vmax=1.08 nmol/min/mg enzyme toward ganciclovir2 Publications

pH dependencei

Optimum pH is 8.5. Active from pH 6 to 8.5.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-HSA-425366 Transport of bile salts and organic acids, metal ions and amine compounds

Protein family/group databases

TCDBi2.A.66.1.14 the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug and toxin extrusion protein 1
Short name:
MATE-1
Short name:
hMATE-1
Alternative name(s):
Solute carrier family 47 member 1
Gene namesi
Name:SLC47A1
Synonyms:MATE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000142494.13
HGNCiHGNC:25588 SLC47A1
MIMi609832 gene
neXtProtiNX_Q96FL8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Topological domaini59 – 72ExtracellularSequence analysisAdd BLAST14
Transmembranei73 – 93HelicalSequence analysisAdd BLAST21
Topological domaini94 – 123CytoplasmicSequence analysisAdd BLAST30
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 152ExtracellularSequence analysis8
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 176CytoplasmicSequence analysis3
Transmembranei177 – 197HelicalSequence analysisAdd BLAST21
Topological domaini198 – 216ExtracellularSequence analysisAdd BLAST19
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Topological domaini238 – 256CytoplasmicSequence analysisAdd BLAST19
Transmembranei257 – 276HelicalSequence analysisAdd BLAST20
Topological domaini277 – 295ExtracellularSequence analysisAdd BLAST19
Transmembranei296 – 316HelicalSequence analysisAdd BLAST21
Topological domaini317 – 336CytoplasmicSequence analysisAdd BLAST20
Transmembranei337 – 357HelicalSequence analysisAdd BLAST21
Topological domaini358 – 370ExtracellularSequence analysisAdd BLAST13
Transmembranei371 – 391HelicalSequence analysisAdd BLAST21
Topological domaini392 – 408CytoplasmicSequence analysisAdd BLAST17
Transmembranei409 – 429HelicalSequence analysisAdd BLAST21
Topological domaini430 – 437ExtracellularSequence analysis8
Transmembranei438 – 458HelicalSequence analysisAdd BLAST21
Topological domaini459 – 546CytoplasmicSequence analysisAdd BLAST88
Transmembranei547 – 567HelicalSequence analysisAdd BLAST21
Topological domaini568 – 570ExtracellularSequence analysis3

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi273E → Q: No change in subcellular location and abolition of MATE1-dependent TEA transport activity. 1 Publication1

Organism-specific databases

DisGeNETi55244
OpenTargetsiENSG00000142494
PharmGKBiPA162403808

Chemistry databases

ChEMBLiCHEMBL1743126
DrugBankiDB00787 Aciclovir
DB01237 Bromodiphenhydramine
DB00567 Cephalexin
DB00501 Cimetidine
DB00331 Metformin
DB00877 Sirolimus
GuidetoPHARMACOLOGYi1216

Polymorphism and mutation databases

BioMutaiSLC47A1
DMDMi74731723

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003128451 – 570Multidrug and toxin extrusion protein 1Add BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ96FL8
PaxDbiQ96FL8
PeptideAtlasiQ96FL8
PRIDEiQ96FL8
ProteomicsDBi76538
76539 [Q96FL8-2]
76540 [Q96FL8-3]

PTM databases

iPTMnetiQ96FL8
PhosphoSitePlusiQ96FL8

Expressioni

Tissue specificityi

Expressed in adrenal gland, and to a lower extent in liver, skeletal muscle and kidney (especially found in luminal membranes of the urinary tubules, bile caniculi and brush border membranes).2 Publications

Gene expression databases

BgeeiENSG00000142494 Expressed in 175 organ(s), highest expression level in adrenal gland
CleanExiHS_SLC47A1
ExpressionAtlasiQ96FL8 baseline and differential
GenevisibleiQ96FL8 HS

Organism-specific databases

HPAiHPA021987

Interactioni

Protein-protein interaction databases

BioGridi120535, 42 interactors
IntActiQ96FL8, 1 interactor
STRINGi9606.ENSP00000270570

Chemistry databases

BindingDBiQ96FL8

Structurei

3D structure databases

ProteinModelPortaliQ96FL8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1347 Eukaryota
COG0534 LUCA
GeneTreeiENSGT00390000015713
HOGENOMiHOG000060313
HOVERGENiHBG056043
InParanoidiQ96FL8
KOiK03327
OMAiLCMEWWA
OrthoDBiEOG091G06X2
PhylomeDBiQ96FL8
TreeFamiTF324441

Family and domain databases

InterProiView protein in InterPro
IPR002528 MATE_fam
PfamiView protein in Pfam
PF01554 MatE, 2 hits
TIGRFAMsiTIGR00797 matE, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q96FL8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEAPEEPAPV RGGPEATLEV RGSRCLRLSA FREELRALLV LAGPAFLVQL
60 70 80 90 100
MVFLISFISS VFCGHLGKLE LDAVTLAIAV INVTGVSVGF GLSSACDTLI
110 120 130 140 150
SQTYGSQNLK HVGVILQRSA LVLLLCCFPC WALFLNTQHI LLLFRQDPDV
160 170 180 190 200
SRLTQTYVTI FIPALPATFL YMLQVKYLLN QGIVLPQIVT GVAANLVNAL
210 220 230 240 250
ANYLFLHQLH LGVIGSALAN LISQYTLALL LFLYILGKKL HQATWGGWSL
260 270 280 290 300
ECLQDWASFL RLAIPSMLML CMEWWAYEVG SFLSGILGMV ELGAQSIVYE
310 320 330 340 350
LAIIVYMVPA GFSVAASVRV GNALGAGDME QARKSSTVSL LITVLFAVAF
360 370 380 390 400
SVLLLSCKDH VGYIFTTDRD IINLVAQVVP IYAVSHLFEA LACTSGGVLR
410 420 430 440 450
GSGNQKVGAI VNTIGYYVVG LPIGIALMFA TTLGVMGLWS GIIICTVFQA
460 470 480 490 500
VCFLGFIIQL NWKKACQQAQ VHANLKVNNV PRSGNSALPQ DPLHPGCPEN
510 520 530 540 550
LEGILTNDVG KTGEPQSDQQ MRQEEPLPEH PQDGAKLSRK QLVLRRGLLL
560 570
LGVFLILLVG ILVRFYVRIQ
Length:570
Mass (Da):61,922
Last modified:December 1, 2001 - v1
Checksum:iCA940CBC2F6F5CC4
GO
Isoform 2 (identifier: Q96FL8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     286-290: ILGMV → LYEDG
     291-570: Missing.

Note: No experimental confirmation available.
Show »
Length:290
Mass (Da):31,907
Checksum:i662AE04699124D14
GO
Isoform 3 (identifier: Q96FL8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     559-570: VGILVRFYVRIQ → AGVRWCDHSSLQPRTLGLQAILLCQPPE

Note: No experimental confirmation available.
Show »
Length:586
Mass (Da):63,549
Checksum:iA1BF0F64D815E7A8
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7EX57E7EX57_HUMAN
Multidrug and toxin extrusion prote...
SLC47A1
443Annotation score:
I3L345I3L345_HUMAN
Multidrug and toxin extrusion prote...
SLC47A1
268Annotation score:
J3KSS8J3KSS8_HUMAN
Multidrug and toxin extrusion prote...
SLC47A1
316Annotation score:
J3KRZ3J3KRZ3_HUMAN
Multidrug and toxin extrusion prote...
SLC47A1
99Annotation score:

Sequence cautioni

The sequence AAH50592 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50L → R in BAD96345 (Ref. 2) Curated1
Sequence conflicti309P → L in BAA91852 (PubMed:14702039).Curated1
Sequence conflicti448F → L in BAD96345 (Ref. 2) Curated1
Sequence conflicti507N → D in BAD96345 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037587338V → I. Corresponds to variant dbSNP:rs35790011Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029903286 – 290ILGMV → LYEDG in isoform 2. 1 Publication5
Alternative sequenceiVSP_029904291 – 570Missing in isoform 2. 1 PublicationAdd BLAST280
Alternative sequenceiVSP_029905559 – 570VGILV…YVRIQ → AGVRWCDHSSLQPRTLGLQA ILLCQPPE in isoform 3. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001709 mRNA Translation: BAA91852.1
AK222625 mRNA Translation: BAD96345.1
CH471212 Genomic DNA Translation: EAW50893.1
CH471212 Genomic DNA Translation: EAW50894.1
BC010661 mRNA Translation: AAH10661.1
BC050592 mRNA Translation: AAH50592.1 Different initiation.
BC058882 mRNA Translation: AAH58882.1
CCDSiCCDS11209.1 [Q96FL8-1]
RefSeqiNP_060712.2, NM_018242.2 [Q96FL8-1]
UniGeneiHs.232054

Genome annotation databases

EnsembliENST00000270570; ENSP00000270570; ENSG00000142494 [Q96FL8-1]
ENST00000395585; ENSP00000378951; ENSG00000142494 [Q96FL8-3]
GeneIDi55244
KEGGihsa:55244
UCSCiuc002gvx.4 human [Q96FL8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001709 mRNA Translation: BAA91852.1
AK222625 mRNA Translation: BAD96345.1
CH471212 Genomic DNA Translation: EAW50893.1
CH471212 Genomic DNA Translation: EAW50894.1
BC010661 mRNA Translation: AAH10661.1
BC050592 mRNA Translation: AAH50592.1 Different initiation.
BC058882 mRNA Translation: AAH58882.1
CCDSiCCDS11209.1 [Q96FL8-1]
RefSeqiNP_060712.2, NM_018242.2 [Q96FL8-1]
UniGeneiHs.232054

3D structure databases

ProteinModelPortaliQ96FL8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120535, 42 interactors
IntActiQ96FL8, 1 interactor
STRINGi9606.ENSP00000270570

Chemistry databases

BindingDBiQ96FL8
ChEMBLiCHEMBL1743126
DrugBankiDB00787 Aciclovir
DB01237 Bromodiphenhydramine
DB00567 Cephalexin
DB00501 Cimetidine
DB00331 Metformin
DB00877 Sirolimus
GuidetoPHARMACOLOGYi1216

Protein family/group databases

TCDBi2.A.66.1.14 the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily

PTM databases

iPTMnetiQ96FL8
PhosphoSitePlusiQ96FL8

Polymorphism and mutation databases

BioMutaiSLC47A1
DMDMi74731723

Proteomic databases

MaxQBiQ96FL8
PaxDbiQ96FL8
PeptideAtlasiQ96FL8
PRIDEiQ96FL8
ProteomicsDBi76538
76539 [Q96FL8-2]
76540 [Q96FL8-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000270570; ENSP00000270570; ENSG00000142494 [Q96FL8-1]
ENST00000395585; ENSP00000378951; ENSG00000142494 [Q96FL8-3]
GeneIDi55244
KEGGihsa:55244
UCSCiuc002gvx.4 human [Q96FL8-1]

Organism-specific databases

CTDi55244
DisGeNETi55244
EuPathDBiHostDB:ENSG00000142494.13
GeneCardsiSLC47A1
H-InvDBiHIX0013619
HGNCiHGNC:25588 SLC47A1
HPAiHPA021987
MIMi609832 gene
neXtProtiNX_Q96FL8
OpenTargetsiENSG00000142494
PharmGKBiPA162403808
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1347 Eukaryota
COG0534 LUCA
GeneTreeiENSGT00390000015713
HOGENOMiHOG000060313
HOVERGENiHBG056043
InParanoidiQ96FL8
KOiK03327
OMAiLCMEWWA
OrthoDBiEOG091G06X2
PhylomeDBiQ96FL8
TreeFamiTF324441

Enzyme and pathway databases

ReactomeiR-HSA-425366 Transport of bile salts and organic acids, metal ions and amine compounds

Miscellaneous databases

ChiTaRSiSLC47A1 human
GeneWikiiSLC47A1
GenomeRNAii55244
PROiPR:Q96FL8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000142494 Expressed in 175 organ(s), highest expression level in adrenal gland
CleanExiHS_SLC47A1
ExpressionAtlasiQ96FL8 baseline and differential
GenevisibleiQ96FL8 HS

Family and domain databases

InterProiView protein in InterPro
IPR002528 MATE_fam
PfamiView protein in Pfam
PF01554 MatE, 2 hits
TIGRFAMsiTIGR00797 matE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiS47A1_HUMAN
AccessioniPrimary (citable) accession number: Q96FL8
Secondary accession number(s): Q53HF5
, Q6PD77, Q86VL4, Q9NVA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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