UniProtKB - Q96FA3 (PELI1_HUMAN)
E3 ubiquitin-protein ligase pellino homolog 1
PELI1
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- ubiquitin protein ligase activity Source: UniProtKB
GO - Biological processi
- interleukin-1-mediated signaling pathway Source: Reactome
- negative regulation of necroptotic process Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: Ensembl
- negative regulation of T cell proliferation Source: Ensembl
- positive regulation of B cell proliferation Source: Ensembl
- positive regulation of cytokine production Source: Ensembl
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Reactome
- positive regulation of protein ubiquitination Source: Ensembl
- positive regulation of toll-like receptor 3 signaling pathway Source: Ensembl
- positive regulation of toll-like receptor 4 signaling pathway Source: Ensembl
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein K48-linked ubiquitination Source: UniProtKB
- protein K63-linked ubiquitination Source: UniProtKB
- protein polyubiquitination Source: GO_Central
- regulation of necroptotic process Source: Reactome
- regulation of Toll signaling pathway Source: InterPro
- response to dsRNA Source: Ensembl
- response to lipopolysaccharide Source: ARUK-UCL
- Toll signaling pathway Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | Q96FA3 |
Reactomei | R-HSA-5675482, Regulation of necroptotic cell death R-HSA-9020702, Interleukin-1 signaling R-HSA-937039, IRAK1 recruits IKK complex R-HSA-975144, IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation |
SignaLinki | Q96FA3 |
SIGNORi | Q96FA3 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase pellino homolog 1 (EC:2.3.2.271 Publication)Short name: Pellino-1 Alternative name(s): Pellino-related intracellular-signaling molecule RING-type E3 ubiquitin transferase pellino homolog 1Curated |
Gene namesi | Name:PELI1 Synonyms:PRISM |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8827, PELI1 |
MIMi | 614797, gene |
neXtProti | NX_Q96FA3 |
VEuPathDBi | HostDB:ENSG00000197329.11 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- condensed nuclear chromosome kinetochore Source: Ensembl
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 104 | R → A: Loss of ability to ubiquitinate RIPK3. Loss of interaction with RIPK1, IRAK1 and RIPK3. 1 Publication | 1 | |
Mutagenesisi | 313 | H → A: Loss of ability to ubiquitinate RIPK3. No loss of interaction with RIPK3. 1 Publication | 1 | |
Mutagenesisi | 336 | C → A: Loss of ability to ubiquitinate RIPK3. No loss of interaction with RIPK3. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 57162 |
OpenTargetsi | ENSG00000197329 |
PharmGKBi | PA33172 |
Miscellaneous databases
Pharosi | Q96FA3, Tbio |
Genetic variation databases
BioMutai | PELI1 |
DMDMi | 37999756 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194172 | 1 – 418 | E3 ubiquitin-protein ligase pellino homolog 1Add BLAST | 418 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q96FA3 |
MassIVEi | Q96FA3 |
MaxQBi | Q96FA3 |
PaxDbi | Q96FA3 |
PeptideAtlasi | Q96FA3 |
PRIDEi | Q96FA3 |
ProteomicsDBi | 76505 |
PTM databases
iPTMneti | Q96FA3 |
PhosphoSitePlusi | Q96FA3 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000197329, Expressed in bone marrow and 238 other tissues |
ExpressionAtlasi | Q96FA3, baseline and differential |
Genevisiblei | Q96FA3, HS |
Organism-specific databases
HPAi | ENSG00000197329, Tissue enhanced (bone) |
Interactioni
Subunit structurei
Interacts with MAP3K7. Upon IL1B treatment, forms a complex with TRAF6, IRAK1, IRAK4 and MYD88; this complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents the complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression (PubMed:12496252, PubMed:12804775, PubMed:16951688).
Interacts (via atypical FHA domain) with RIPK3; preferentially binds to the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609).
Interacts with RIPK1 and IRAK1 (PubMed:29883609).
4 PublicationsBinary interactionsi
Hide detailsQ96FA3
Protein-protein interaction databases
BioGRIDi | 121418, 54 interactors |
CORUMi | Q96FA3 |
DIPi | DIP-32488N |
IntActi | Q96FA3, 28 interactors |
MINTi | Q96FA3 |
STRINGi | 9606.ENSP00000351789 |
Miscellaneous databases
RNActi | Q96FA3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 200 | FHA; atypicalBy similarityAdd BLAST | 188 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 311 – 399 | Ring-like domain; necessary for ubiqitination of RIPK31 PublicationAdd BLAST | 89 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3842, Eukaryota |
GeneTreei | ENSGT00950000183050 |
HOGENOMi | CLU_029221_2_0_1 |
InParanoidi | Q96FA3 |
OMAi | TFAFNPC |
OrthoDBi | 837213at2759 |
PhylomeDBi | Q96FA3 |
TreeFami | TF314338 |
Family and domain databases
InterProi | View protein in InterPro IPR006800, Pellino_fam |
PANTHERi | PTHR12098, PTHR12098, 1 hit |
Pfami | View protein in Pfam PF04710, Pellino, 1 hit |
PIRSFi | PIRSF038886, Pellino, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MFSPDQENHP SKAPVKYGEL IVLGYNGSLP NGDRGRRKSR FALFKRPKAN
60 70 80 90 100
GVKPSTVHIA CTPQAAKAIS NKDQHSISYT LSRAQTVVVE YTHDSNTDMF
110 120 130 140 150
QIGRSTESPI DFVVTDTVPG SQSNSDTQSV QSTISRFACR IICERNPPFT
160 170 180 190 200
ARIYAAGFDS SKNIFLGEKA AKWKTSDGQM DGLTTNGVLV MHPRNGFTED
210 220 230 240 250
SKPGIWREIS VCGNVFSLRE TRSAQQRGKM VEIETNQLQD GSLIDLCGAT
260 270 280 290 300
LLWRTAEGLS HTPTVKHLEA LRQEINAARP QCPVGFNTLA FPSMKRKDVV
310 320 330 340 350
DEKQPWVYLN CGHVHGYHNW GNKEERDGKD RECPMCRSVG PYVPLWLGCE
360 370 380 390 400
AGFYVDAGPP THAFSPCGHV CSEKTTAYWS QIPLPHGTHT FHAACPFCAH
410
QLAGEQGYIR LIFQGPLD
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 11 | S → F in CAC04320 (PubMed:11132151).Curated | 1 | |
Sequence conflicti | 100 | F → S in BAB55280 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 260 | S → P in BAB55280 (PubMed:14702039).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278859 mRNA Translation: CAC04320.1 AF302505 mRNA Translation: AAG15393.1 AF300987 mRNA Translation: AAG17451.1 BC011419 mRNA Translation: AAH11419.1 Different initiation. BC050019 mRNA Translation: AAH50019.1 BC063611 mRNA Translation: AAH63611.1 AK027668 mRNA Translation: BAB55280.1 Different initiation. |
CCDSi | CCDS1876.1 |
RefSeqi | NP_065702.2, NM_020651.3 XP_011531296.1, XM_011532994.2 XP_016860009.1, XM_017004520.1 |
Genome annotation databases
Ensembli | ENST00000358912; ENSP00000351789; ENSG00000197329 |
GeneIDi | 57162 |
KEGGi | hsa:57162 |
UCSCi | uc002sct.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278859 mRNA Translation: CAC04320.1 AF302505 mRNA Translation: AAG15393.1 AF300987 mRNA Translation: AAG17451.1 BC011419 mRNA Translation: AAH11419.1 Different initiation. BC050019 mRNA Translation: AAH50019.1 BC063611 mRNA Translation: AAH63611.1 AK027668 mRNA Translation: BAB55280.1 Different initiation. |
CCDSi | CCDS1876.1 |
RefSeqi | NP_065702.2, NM_020651.3 XP_011531296.1, XM_011532994.2 XP_016860009.1, XM_017004520.1 |
3D structure databases
SMRi | Q96FA3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 121418, 54 interactors |
CORUMi | Q96FA3 |
DIPi | DIP-32488N |
IntActi | Q96FA3, 28 interactors |
MINTi | Q96FA3 |
STRINGi | 9606.ENSP00000351789 |
PTM databases
iPTMneti | Q96FA3 |
PhosphoSitePlusi | Q96FA3 |
Genetic variation databases
BioMutai | PELI1 |
DMDMi | 37999756 |
Proteomic databases
EPDi | Q96FA3 |
MassIVEi | Q96FA3 |
MaxQBi | Q96FA3 |
PaxDbi | Q96FA3 |
PeptideAtlasi | Q96FA3 |
PRIDEi | Q96FA3 |
ProteomicsDBi | 76505 |
Protocols and materials databases
Antibodypediai | 16046, 227 antibodies |
DNASUi | 57162 |
Genome annotation databases
Ensembli | ENST00000358912; ENSP00000351789; ENSG00000197329 |
GeneIDi | 57162 |
KEGGi | hsa:57162 |
UCSCi | uc002sct.5, human |
Organism-specific databases
CTDi | 57162 |
DisGeNETi | 57162 |
GeneCardsi | PELI1 |
HGNCi | HGNC:8827, PELI1 |
HPAi | ENSG00000197329, Tissue enhanced (bone) |
MIMi | 614797, gene |
neXtProti | NX_Q96FA3 |
OpenTargetsi | ENSG00000197329 |
PharmGKBi | PA33172 |
VEuPathDBi | HostDB:ENSG00000197329.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3842, Eukaryota |
GeneTreei | ENSGT00950000183050 |
HOGENOMi | CLU_029221_2_0_1 |
InParanoidi | Q96FA3 |
OMAi | TFAFNPC |
OrthoDBi | 837213at2759 |
PhylomeDBi | Q96FA3 |
TreeFami | TF314338 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q96FA3 |
Reactomei | R-HSA-5675482, Regulation of necroptotic cell death R-HSA-9020702, Interleukin-1 signaling R-HSA-937039, IRAK1 recruits IKK complex R-HSA-975144, IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation |
SignaLinki | Q96FA3 |
SIGNORi | Q96FA3 |
Miscellaneous databases
BioGRID-ORCSi | 57162, 7 hits in 1000 CRISPR screens |
ChiTaRSi | PELI1, human |
GeneWikii | PELI1 |
GenomeRNAii | 57162 |
Pharosi | Q96FA3, Tbio |
PROi | PR:Q96FA3 |
RNActi | Q96FA3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000197329, Expressed in bone marrow and 238 other tissues |
ExpressionAtlasi | Q96FA3, baseline and differential |
Genevisiblei | Q96FA3, HS |
Family and domain databases
InterProi | View protein in InterPro IPR006800, Pellino_fam |
PANTHERi | PTHR12098, PTHR12098, 1 hit |
Pfami | View protein in Pfam PF04710, Pellino, 1 hit |
PIRSFi | PIRSF038886, Pellino, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PELI1_HUMAN | |
Accessioni | Q96FA3Primary (citable) accession number: Q96FA3 Secondary accession number(s): Q96SM0, Q9GZY5, Q9HCX0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 24, 2003 |
Last sequence update: | October 24, 2003 | |
Last modified: | April 7, 2021 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families