UniProtKB - Q96F24 (NRBF2_HUMAN)
Nuclear receptor-binding factor 2
NRBF2
Functioni
May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).
1 PublicationInvolved in starvation-induced autophagy probably by its association with PI3K complex I (PI3KC3-C1). However, effects has been described variably. Involved in the induction of starvation-induced autophagy (PubMed:24785657).
Stabilzes PI3KC3-C1 assembly and enhances ATG14-linked lipid kinase activity of PIK3C3 (By similarity).
Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1 (PubMed:25086043).
May be involved in autophagosome biogenesis (PubMed:25086043).
May play a role in neural progenitor cell survival during differentiation (By similarity).
By similarity2 PublicationsGO - Biological processi
- autophagy Source: GO_Central
- regulation of lipid kinase activity Source: Ensembl
- response to endoplasmic reticulum stress Source: Ensembl
Keywordsi
Biological process | Autophagy, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q96F24 |
Reactomei | R-HSA-383280, Nuclear Receptor transcription pathway |
SignaLinki | Q96F24 |
SIGNORi | Q96F24 |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear receptor-binding factor 2Short name: NRBF-2 Alternative name(s): Comodulator of PPAR and RXR |
Gene namesi | Name:NRBF2 Synonyms:COPR |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19692, NRBF2 |
MIMi | 616477, gene |
neXtProti | NX_Q96F24 |
VEuPathDBi | HostDB:ENSG00000148572 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- Cytoplasmic vesicle 1 Publication
- autophagosome Curated
Nucleus
- nucleoplasm Source: Reactome
Other locations
- autophagosome Source: UniProtKB-SubCell
- cytoplasm Source: LIFEdb
- cytoplasmic vesicle Source: UniProtKB-KW
- phosphatidylinositol 3-kinase complex, class III Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 144 – 145 | LL → AA: Decreased interaction with nuclear receptors. 1 Publication | 2 |
Organism-specific databases
DisGeNETi | 29982 |
OpenTargetsi | ENSG00000148572 |
PharmGKBi | PA134946285 |
Miscellaneous databases
Pharosi | Q96F24, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295924 |
Genetic variation databases
BioMutai | NRBF2 |
DMDMi | 74731648 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000235816 | 1 – 287 | Nuclear receptor-binding factor 2Add BLAST | 287 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 113 | PhosphoserineCombined sources | 1 | |
Modified residuei | 268 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q96F24 |
jPOSTi | Q96F24 |
MassIVEi | Q96F24 |
MaxQBi | Q96F24 |
PaxDbi | Q96F24 |
PeptideAtlasi | Q96F24 |
PRIDEi | Q96F24 |
ProteomicsDBi | 5374 76486 [Q96F24-1] 76487 [Q96F24-2] |
PTM databases
GlyGeni | Q96F24, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96F24 |
PhosphoSitePlusi | Q96F24 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000148572, Expressed in blood and 231 other tissues |
Genevisiblei | Q96F24, HS |
Organism-specific databases
HPAi | ENSG00000148572, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with PPARA, PPARD and PPARG.
Interacts with RARA, RARG and RXRA in the presence of bound ligand (PubMed:15610520).
Interacts with SCOC (PubMed:22354037). Associates with the PI3K complex I (PI3KC3-C1); the direct binding partner in the complex is reported variably as PIK3R4 or ATG14 (PubMed:24785657).
4 PublicationsBinary interactionsi
Q96F24
Protein-protein interaction databases
BioGRIDi | 119009, 58 interactors |
IntActi | Q96F24, 29 interactors |
MINTi | Q96F24 |
STRINGi | 9606.ENSP00000277746 |
Miscellaneous databases
RNActi | Q96F24, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q96F24 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 83 – 122 | DisorderedSequence analysisAdd BLAST | 40 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 168 – 209 | Sequence analysisAdd BLAST | 42 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 141 – 145 | Nuclear receptor interaction motif | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 105 – 122 | Polar residuesSequence analysisAdd BLAST | 18 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QRE0, Eukaryota |
GeneTreei | ENSGT00390000000984 |
HOGENOMi | CLU_098323_0_0_1 |
InParanoidi | Q96F24 |
OMAi | TWQKFSA |
OrthoDBi | 1613014at2759 |
PhylomeDBi | Q96F24 |
TreeFami | TF328627 |
Family and domain databases
IDEALi | IID00676 |
InterProi | View protein in InterPro IPR039679, NRBF2 IPR015056, NRBF2_C IPR033393, NRBF2_MIT |
PANTHERi | PTHR14964, PTHR14964, 1 hit |
Pfami | View protein in Pfam PF08961, NRBF2, 1 hit PF17169, NRBF2_MIT, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEVMEGPLNL AHQQSRRADR LLAAGKYEEA ISCHKKAAAY LSEAMKLTQS
60 70 80 90 100
EQAHLSLELQ RDSHMKQLLL IQERWKRAQR EERLKAQQNT DKDAAAHLQT
110 120 130 140 150
SHKPSAEDAE GQSPLSQKYS PSTEKCLPEI QGIFDRDPDT LLYLLQQKSE
160 170 180 190 200
PAEPCIGSKA PKDDKTIIEE QATKIADLKR HVEFLVAENE RLRKENKQLK
210 220 230 240 250
AEKARLLKGP IEKELDVDAD FVETSELWSL PPHAETATAS STWQKFAANT
260 270 280
GKAKDIPIPN LPPLDFPSPE LPLMELSEDI LKGFMNN
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 150 | E → G in BAB70833 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 284 | F → L in CAB66591 (PubMed:11230166).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054555 | 1 – 38 | MEVME…HKKAA → MFPGATTPLPKAAAYPGVYG SNGRTPQP in isoform 3. 1 PublicationAdd BLAST | 38 | |
Alternative sequenceiVSP_018488 | 90 – 139 | Missing in isoform 2. 1 PublicationAdd BLAST | 50 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY267839 mRNA Translation: AAP03081.1 AY267840 mRNA Translation: AAP03082.1 AF267866 mRNA Translation: AAG44735.1 Frameshift. AL136656 mRNA Translation: CAB66591.1 AK054957 mRNA Translation: BAB70833.1 AK301656 mRNA Translation: BAG63132.1 AL590502 Genomic DNA No translation available. CH471083 Genomic DNA Translation: EAW54241.1 BC011707 mRNA Translation: AAH11707.1 |
CCDSi | CCDS60537.1 [Q96F24-3] CCDS7268.1 [Q96F24-1] |
RefSeqi | NP_001269334.1, NM_001282405.1 [Q96F24-3] NP_110386.2, NM_030759.4 [Q96F24-1] |
Genome annotation databases
Ensembli | ENST00000277746; ENSP00000277746; ENSG00000148572 ENST00000435510; ENSP00000397502; ENSG00000148572 [Q96F24-3] |
GeneIDi | 29982 |
KEGGi | hsa:29982 |
MANE-Selecti | ENST00000277746.11; ENSP00000277746.6; NM_030759.5; NP_110386.2 |
UCSCi | uc001jmj.6, human [Q96F24-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY267839 mRNA Translation: AAP03081.1 AY267840 mRNA Translation: AAP03082.1 AF267866 mRNA Translation: AAG44735.1 Frameshift. AL136656 mRNA Translation: CAB66591.1 AK054957 mRNA Translation: BAB70833.1 AK301656 mRNA Translation: BAG63132.1 AL590502 Genomic DNA No translation available. CH471083 Genomic DNA Translation: EAW54241.1 BC011707 mRNA Translation: AAH11707.1 |
CCDSi | CCDS60537.1 [Q96F24-3] CCDS7268.1 [Q96F24-1] |
RefSeqi | NP_001269334.1, NM_001282405.1 [Q96F24-3] NP_110386.2, NM_030759.4 [Q96F24-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4ZEY | X-ray | 1.50 | A | 4-86 | [»] | |
SMRi | Q96F24 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119009, 58 interactors |
IntActi | Q96F24, 29 interactors |
MINTi | Q96F24 |
STRINGi | 9606.ENSP00000277746 |
Chemistry databases
ChEMBLi | CHEMBL4295924 |
PTM databases
GlyGeni | Q96F24, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96F24 |
PhosphoSitePlusi | Q96F24 |
Genetic variation databases
BioMutai | NRBF2 |
DMDMi | 74731648 |
Proteomic databases
EPDi | Q96F24 |
jPOSTi | Q96F24 |
MassIVEi | Q96F24 |
MaxQBi | Q96F24 |
PaxDbi | Q96F24 |
PeptideAtlasi | Q96F24 |
PRIDEi | Q96F24 |
ProteomicsDBi | 5374 76486 [Q96F24-1] 76487 [Q96F24-2] |
Protocols and materials databases
Antibodypediai | 28311, 302 antibodies from 30 providers |
DNASUi | 29982 |
Genome annotation databases
Ensembli | ENST00000277746; ENSP00000277746; ENSG00000148572 ENST00000435510; ENSP00000397502; ENSG00000148572 [Q96F24-3] |
GeneIDi | 29982 |
KEGGi | hsa:29982 |
MANE-Selecti | ENST00000277746.11; ENSP00000277746.6; NM_030759.5; NP_110386.2 |
UCSCi | uc001jmj.6, human [Q96F24-1] |
Organism-specific databases
CTDi | 29982 |
DisGeNETi | 29982 |
GeneCardsi | NRBF2 |
HGNCi | HGNC:19692, NRBF2 |
HPAi | ENSG00000148572, Low tissue specificity |
MIMi | 616477, gene |
neXtProti | NX_Q96F24 |
OpenTargetsi | ENSG00000148572 |
PharmGKBi | PA134946285 |
VEuPathDBi | HostDB:ENSG00000148572 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QRE0, Eukaryota |
GeneTreei | ENSGT00390000000984 |
HOGENOMi | CLU_098323_0_0_1 |
InParanoidi | Q96F24 |
OMAi | TWQKFSA |
OrthoDBi | 1613014at2759 |
PhylomeDBi | Q96F24 |
TreeFami | TF328627 |
Enzyme and pathway databases
PathwayCommonsi | Q96F24 |
Reactomei | R-HSA-383280, Nuclear Receptor transcription pathway |
SignaLinki | Q96F24 |
SIGNORi | Q96F24 |
Miscellaneous databases
BioGRID-ORCSi | 29982, 109 hits in 985 CRISPR screens |
ChiTaRSi | NRBF2, human |
GeneWikii | NRBF2 |
GenomeRNAii | 29982 |
Pharosi | Q96F24, Tbio |
PROi | PR:Q96F24 |
RNActi | Q96F24, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000148572, Expressed in blood and 231 other tissues |
Genevisiblei | Q96F24, HS |
Family and domain databases
IDEALi | IID00676 |
InterProi | View protein in InterPro IPR039679, NRBF2 IPR015056, NRBF2_C IPR033393, NRBF2_MIT |
PANTHERi | PTHR14964, PTHR14964, 1 hit |
Pfami | View protein in Pfam PF08961, NRBF2, 1 hit PF17169, NRBF2_MIT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NRBF2_HUMAN | |
Accessioni | Q96F24Primary (citable) accession number: Q96F24 Secondary accession number(s): A6PW36 Q9H2I2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references