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Protein

Microspherule protein 1

Gene

MCRS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus (PubMed:11948183). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. May also be an inhibitor of TERT telomerase activity (PubMed:15044100). Binds to G-quadruplex structures in mRNA (PubMed:16571602). Binds to RNA homopolymer poly(G) and poly(U) (PubMed:16571602).4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator
Biological processDNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3214847 HATs acetylate histones
R-HSA-5689603 UCH proteinases
R-HSA-5696394 DNA Damage Recognition in GG-NER

Names & Taxonomyi

Protein namesi
Recommended name:
Microspherule protein 1
Alternative name(s):
58 kDa microspherule protein
Cell cycle-regulated factor p78
INO80 complex subunit J
MCRS2
Gene namesi
Name:MCRS1
Synonyms:INO80Q, MSP58
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000187778.13
HGNCiHGNC:6960 MCRS1
MIMi609504 gene
neXtProtiNX_Q96EZ8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10445
OpenTargetsiENSG00000187778
PharmGKBiPA30708

Polymorphism and mutation databases

BioMutaiMCRS1
DMDMi24638035

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000963051 – 462Microspherule protein 1Add BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei22PhosphoserineCombined sources1
Modified residuei102PhosphoserineBy similarity1
Modified residuei103PhosphothreonineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei123N6-acetyllysineCombined sources1
Modified residuei130N6-acetyllysineCombined sources1
Modified residuei282PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96EZ8
MaxQBiQ96EZ8
PaxDbiQ96EZ8
PeptideAtlasiQ96EZ8
PRIDEiQ96EZ8
ProteomicsDBi76477
76478 [Q96EZ8-2]
76479 [Q96EZ8-3]

PTM databases

iPTMnetiQ96EZ8
PhosphoSitePlusiQ96EZ8
SwissPalmiQ96EZ8

Expressioni

Tissue specificityi

Detected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes.

Developmental stagei

Cell-cycle regulated: levels are highest early in S phase; not detectable in G2.

Gene expression databases

BgeeiENSG00000187778 Expressed in 216 organ(s), highest expression level in apex of heart
CleanExiHS_MCRS1
ExpressionAtlasiQ96EZ8 baseline and differential
GenevisibleiQ96EZ8 HS

Organism-specific databases

HPAiHPA039057

Interactioni

Subunit structurei

Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the N-terminus of INO80 (PubMed:16230350, PubMed:18922472, PubMed:21303910). Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10 (PubMed:15960975). Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1 (PubMed:20018852). Interacts with NOP2 (PubMed:9654073). Interacts with PINX1 (PubMed:15044100). Interacts with TERT (PubMed:15044100). Interacts with CCDC85B (PubMed:17014843). Interacts with DAXX (PubMed:11948183). Interacts (via N-terminus) with FMR1 (via phosphorylated form) (PubMed:16571602). Interacts with FXR1 AND FXR2 (PubMed:16571602).10 Publications
(Microbial infection) Interacts with Herpes simplex virus ICP22.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi115710, 78 interactors
ComplexPortaliCPX-809 NSL histone acetyltransferase complex
CPX-846 INO80 chromatin remodeling complex
CORUMiQ96EZ8
IntActiQ96EZ8, 128 interactors
MINTiQ96EZ8
STRINGi9606.ENSP00000349640

Structurei

3D structure databases

ProteinModelPortaliQ96EZ8
SMRiQ96EZ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini363 – 419FHAPROSITE-ProRule annotationAdd BLAST57

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili301 – 335Sequence analysisAdd BLAST35

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi113 – 123Nuclear localization signalSequence analysisAdd BLAST11

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 102Ser-richAdd BLAST92

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2293 Eukaryota
ENOG410XS0S LUCA
GeneTreeiENSGT00390000005536
HOGENOMiHOG000007227
HOVERGENiHBG052434
InParanoidiQ96EZ8
KOiK11674
OMAiFLVNYEL
OrthoDBiEOG091G07QN
PhylomeDBiQ96EZ8
TreeFamiTF318119

Family and domain databases

CDDicd00060 FHA, 1 hit
InterProiView protein in InterPro
IPR000253 FHA_dom
IPR037912 MCRS1
IPR025999 MCRS_N
IPR008984 SMAD_FHA_dom_sf
PANTHERiPTHR13233 PTHR13233, 1 hit
PfamiView protein in Pfam
PF00498 FHA, 1 hit
PF13325 MCRS_N, 1 hit
SMARTiView protein in SMART
SM00240 FHA, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
PROSITEiView protein in PROSITE
PS50006 FHA_DOMAIN, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96EZ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDKDSQGLLD SSLMASGTAS RSEDEESLAG QKRASSQALG TIPKRRSSSR
60 70 80 90 100
FIKRKKFDDE LVESSLAKSS TRAKGASGVE PGRCSGSEPS SSEKKKVSKA
110 120 130 140 150
PSTPVPPSPA PAPGLTKRVK KSKQPLQVTK DLGRWKPADD LLLINAVLQT
160 170 180 190 200
NDLTSVHLGV KFSCRFTLRE VQERWYALLY DPVISKLACQ AMRQLHPEAI
210 220 230 240 250
AAIQSKALFS KAEEQLLSKV GSTSQPTLET FQDLLHRHPD AFYLARTAKA
260 270 280 290 300
LQAHWQLMKQ YYLLEDQTVQ PLPKGDQVLN FSDAEDLIDD SKLKDMRDEV
310 320 330 340 350
LEHELMVADR RQKREIRQLE QELHKWQVLV DSITGMSSPD FDNQTLAVLR
360 370 380 390 400
GRMVRYLMRS REITLGRATK DNQIDVDLSL EGPAWKISRK QGVIKLKNNG
410 420 430 440 450
DFFIANEGRR PIYIDGRPVL CGSKWRLSNN SVVEIASLRF VFLINQDLIA
460
LIRAEAAKIT PQ
Length:462
Mass (Da):51,803
Last modified:December 1, 2001 - v1
Checksum:iF6B7CC8A2AAF16BC
GO
Isoform 2 (identifier: Q96EZ8-2) [UniParc]FASTAAdd to basket
Also known as: MCRS2

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MDK → MTRGTGGTAQRGRSGP

Show »
Length:475
Mass (Da):53,001
Checksum:i5ABAFCB412408F76
GO
Isoform 3 (identifier: Q96EZ8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Note: No experimental confirmation available. May be due to intron retention.
Show »
Length:449
Mass (Da):50,413
Checksum:i46DB62C0D3158EC1
GO
Isoform 4 (identifier: Q96EZ8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-191: Missing.

Note: No experimental confirmation available.
Show »
Length:271
Mass (Da):31,134
Checksum:i9B6338AC1B6BAF5E
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8W126F8W126_HUMAN
Microspherule protein 1
MCRS1
318Annotation score:
H0YIA0H0YIA0_HUMAN
Microspherule protein 1
MCRS1
173Annotation score:
F8VVA6F8VVA6_HUMAN
Microspherule protein 1
MCRS1
185Annotation score:
F8VP44F8VP44_HUMAN
Microspherule protein 1
MCRS1
205Annotation score:
F8W0I9F8W0I9_HUMAN
Microspherule protein 1
MCRS1
144Annotation score:
H0YI67H0YI67_HUMAN
Microspherule protein 1
MCRS1
64Annotation score:
F8VZC2F8VZC2_HUMAN
Microspherule protein 1
MCRS1
61Annotation score:

Sequence cautioni

The sequence AAC68599 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245A → G in AAC52086 (PubMed:9654073).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035473441V → I in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs780785469Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0545711 – 191Missing in isoform 4. CuratedAdd BLAST191
Alternative sequenceiVSP_0162591 – 13Missing in isoform 3. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_0162601 – 3MDK → MTRGTGGTAQRGRSGP in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015308 mRNA Translation: AAC52086.1
AF068007 mRNA Translation: AAC68599.1 Frameshift.
AY336730 mRNA Translation: AAQ84517.1
BX538079 mRNA Translation: CAD98003.1
AC020612 Genomic DNA No translation available.
CH471111 Genomic DNA Translation: EAW58078.1
BC011794 mRNA Translation: AAH11794.1
CCDSiCCDS31795.1 [Q96EZ8-2]
CCDS61118.1 [Q96EZ8-4]
CCDS8787.1 [Q96EZ8-1]
RefSeqiNP_001012300.1, NM_001012300.1 [Q96EZ8-2]
NP_001265270.1, NM_001278341.1 [Q96EZ8-4]
NP_006328.2, NM_006337.4 [Q96EZ8-1]
XP_005268629.1, XM_005268572.3 [Q96EZ8-1]
XP_016874178.1, XM_017018689.1 [Q96EZ8-2]
XP_016874179.1, XM_017018690.1 [Q96EZ8-2]
UniGeneiHs.25313

Genome annotation databases

EnsembliENST00000343810; ENSP00000345358; ENSG00000187778 [Q96EZ8-1]
ENST00000357123; ENSP00000349640; ENSG00000187778 [Q96EZ8-2]
ENST00000546244; ENSP00000444982; ENSG00000187778 [Q96EZ8-4]
ENST00000550165; ENSP00000448056; ENSG00000187778 [Q96EZ8-1]
GeneIDi10445
KEGGihsa:10445
UCSCiuc001rui.3 human [Q96EZ8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015308 mRNA Translation: AAC52086.1
AF068007 mRNA Translation: AAC68599.1 Frameshift.
AY336730 mRNA Translation: AAQ84517.1
BX538079 mRNA Translation: CAD98003.1
AC020612 Genomic DNA No translation available.
CH471111 Genomic DNA Translation: EAW58078.1
BC011794 mRNA Translation: AAH11794.1
CCDSiCCDS31795.1 [Q96EZ8-2]
CCDS61118.1 [Q96EZ8-4]
CCDS8787.1 [Q96EZ8-1]
RefSeqiNP_001012300.1, NM_001012300.1 [Q96EZ8-2]
NP_001265270.1, NM_001278341.1 [Q96EZ8-4]
NP_006328.2, NM_006337.4 [Q96EZ8-1]
XP_005268629.1, XM_005268572.3 [Q96EZ8-1]
XP_016874178.1, XM_017018689.1 [Q96EZ8-2]
XP_016874179.1, XM_017018690.1 [Q96EZ8-2]
UniGeneiHs.25313

3D structure databases

ProteinModelPortaliQ96EZ8
SMRiQ96EZ8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115710, 78 interactors
ComplexPortaliCPX-809 NSL histone acetyltransferase complex
CPX-846 INO80 chromatin remodeling complex
CORUMiQ96EZ8
IntActiQ96EZ8, 128 interactors
MINTiQ96EZ8
STRINGi9606.ENSP00000349640

PTM databases

iPTMnetiQ96EZ8
PhosphoSitePlusiQ96EZ8
SwissPalmiQ96EZ8

Polymorphism and mutation databases

BioMutaiMCRS1
DMDMi24638035

Proteomic databases

EPDiQ96EZ8
MaxQBiQ96EZ8
PaxDbiQ96EZ8
PeptideAtlasiQ96EZ8
PRIDEiQ96EZ8
ProteomicsDBi76477
76478 [Q96EZ8-2]
76479 [Q96EZ8-3]

Protocols and materials databases

DNASUi10445
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000343810; ENSP00000345358; ENSG00000187778 [Q96EZ8-1]
ENST00000357123; ENSP00000349640; ENSG00000187778 [Q96EZ8-2]
ENST00000546244; ENSP00000444982; ENSG00000187778 [Q96EZ8-4]
ENST00000550165; ENSP00000448056; ENSG00000187778 [Q96EZ8-1]
GeneIDi10445
KEGGihsa:10445
UCSCiuc001rui.3 human [Q96EZ8-1]

Organism-specific databases

CTDi10445
DisGeNETi10445
EuPathDBiHostDB:ENSG00000187778.13
GeneCardsiMCRS1
HGNCiHGNC:6960 MCRS1
HPAiHPA039057
MIMi609504 gene
neXtProtiNX_Q96EZ8
OpenTargetsiENSG00000187778
PharmGKBiPA30708
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2293 Eukaryota
ENOG410XS0S LUCA
GeneTreeiENSGT00390000005536
HOGENOMiHOG000007227
HOVERGENiHBG052434
InParanoidiQ96EZ8
KOiK11674
OMAiFLVNYEL
OrthoDBiEOG091G07QN
PhylomeDBiQ96EZ8
TreeFamiTF318119

Enzyme and pathway databases

ReactomeiR-HSA-3214847 HATs acetylate histones
R-HSA-5689603 UCH proteinases
R-HSA-5696394 DNA Damage Recognition in GG-NER

Miscellaneous databases

ChiTaRSiMCRS1 human
GeneWikiiMCRS1
GenomeRNAii10445
PROiPR:Q96EZ8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000187778 Expressed in 216 organ(s), highest expression level in apex of heart
CleanExiHS_MCRS1
ExpressionAtlasiQ96EZ8 baseline and differential
GenevisibleiQ96EZ8 HS

Family and domain databases

CDDicd00060 FHA, 1 hit
InterProiView protein in InterPro
IPR000253 FHA_dom
IPR037912 MCRS1
IPR025999 MCRS_N
IPR008984 SMAD_FHA_dom_sf
PANTHERiPTHR13233 PTHR13233, 1 hit
PfamiView protein in Pfam
PF00498 FHA, 1 hit
PF13325 MCRS_N, 1 hit
SMARTiView protein in SMART
SM00240 FHA, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
PROSITEiView protein in PROSITE
PS50006 FHA_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMCRS1_HUMAN
AccessioniPrimary (citable) accession number: Q96EZ8
Secondary accession number(s): O14742
, O75497, Q6VN53, Q7Z372
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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