UniProtKB - Q96EY1 (DNJA3_HUMAN)
DnaJ homolog subfamily A member 3, mitochondrial
DNAJA3
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 236 | Zinc 1Combined sources1 Publication | 1 | |
Metal bindingi | 239 | Zinc 1Combined sources1 Publication | 1 | |
Metal bindingi | 253 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 256 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 275 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 278 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 289 | Zinc 1Combined sources1 Publication | 1 | |
Metal bindingi | 292 | Zinc 1Combined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 223 – 301 | CR-typeAdd BLAST | 79 |
GO - Molecular functioni
- ATP binding Source: InterPro
- GTPase regulator activity Source: Ensembl
- Hsp70 protein binding Source: Ensembl
- IkappaB kinase complex binding Source: UniProtKB
- interferon-gamma receptor binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- NF-kappaB binding Source: UniProtKB
- protein-containing complex binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
- transcription factor binding Source: UniProtKB
- unfolded protein binding Source: Ensembl
GO - Biological processi
- activation-induced cell death of T cells Source: Ensembl
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- cell aging Source: Ensembl
- mitochondrial DNA replication Source: Ensembl
- mitochondrion organization Source: GO_Central
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- negative regulation of interferon-gamma-mediated signaling pathway Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- negative regulation of programmed cell death Source: Ensembl
- negative regulation of protein kinase activity Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- neuromuscular junction development Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of protein ubiquitination Source: UniProtKB
- positive regulation of T cell proliferation Source: Ensembl
- protein folding Source: UniProtKB
- protein stabilization Source: UniProtKB
- response to heat Source: InterPro
- response to interferon-gamma Source: UniProtKB
- skeletal muscle acetylcholine-gated channel clustering Source: UniProtKB
- small GTPase mediated signal transduction Source: Ensembl
- T cell differentiation in thymus Source: Ensembl
Keywordsi
Molecular function | Chaperone |
Biological process | Apoptosis |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: DnaJ homolog subfamily A member 3, mitochondrialAlternative name(s): DnaJ protein Tid-1 Short name: hTid-1 Hepatocellular carcinoma-associated antigen 57 Tumorous imaginal discs protein Tid56 homolog |
Gene namesi | Name:DNAJA3 Synonyms:HCA57, TID1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11808 DNAJA3 |
MIMi | 608382 gene |
neXtProti | NX_Q96EY1 |
Subcellular locationi
Plasma membrane
- postsynaptic cell membrane By similarity; Peripheral membrane protein By similarity
Mitochondrion
Cytosol
- cytosol By similarity
Note: Recruited to the postsynaptic cell membrane of the neuromuscular junction through interaction with MUSK.By similarity
Cytosol
- cytosol Source: UniProtKB
Mitochondrion
- mitochondrial matrix Source: UniProtKB
- mitochondrial nucleoid Source: UniProtKB
- mitochondrion Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- extrinsic component of plasma membrane Source: UniProtKB
- postsynaptic membrane Source: UniProtKB
Other locations
- cell junction Source: UniProtKB-KW
- cytoplasm Source: UniProtKB
- intracellular membrane-bounded organelle Source: HPA
- neuromuscular junction Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Membrane, Mitochondrion, Postsynaptic cell membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 121 | H → Q: Loss of modulation of apoptosis. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 9093 |
OpenTargetsi | ENSG00000103423 |
PharmGKBi | PA27410 |
Miscellaneous databases
Pharosi | Q96EY1 |
Polymorphism and mutation databases
BioMutai | DNAJA3 |
DMDMi | 311033374 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000007256 | ? – 480 | DnaJ homolog subfamily A member 3, mitochondrial |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 58 | Omega-N-methylarginine; by CARM11 Publication | 1 | |
Modified residuei | 134 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 238 | Omega-N-methylarginine; by CARM11 Publication | 1 | |
Modified residuei | 293 | Omega-N-methylarginine; by CARM11 Publication | 1 | |
Modified residuei | 398 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | Q96EY1 |
jPOSTi | Q96EY1 |
MassIVEi | Q96EY1 |
MaxQBi | Q96EY1 |
PaxDbi | Q96EY1 |
PeptideAtlasi | Q96EY1 |
PRIDEi | Q96EY1 |
ProteomicsDBi | 17904 76466 [Q96EY1-1] 76467 [Q96EY1-2] |
PTM databases
iPTMneti | Q96EY1 |
PhosphoSitePlusi | Q96EY1 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000103423 Expressed in 224 organ(s), highest expression level in adrenal gland |
ExpressionAtlasi | Q96EY1 baseline and differential |
Genevisiblei | Q96EY1 HS |
Organism-specific databases
HPAi | CAB016095 HPA040875 HPA044229 |
Interactioni
Subunit structurei
Interacts with JAK2, HSPA9B and IFN-gammaR2 chain.
Interacts with Ras GTPase-activating protein 1 (RASA1).
Isoform 2 interacts with MUSK (via the cytoplasmic domain) (By similarity).
By similarityBinary interactionsi
GO - Molecular functioni
- Hsp70 protein binding Source: Ensembl
- interferon-gamma receptor binding Source: UniProtKB
- NF-kappaB binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
- transcription factor binding Source: UniProtKB
- unfolded protein binding Source: Ensembl
Protein-protein interaction databases
BioGridi | 114547, 121 interactors |
DIPi | DIP-33870N |
IntActi | Q96EY1, 78 interactors |
MINTi | Q96EY1 |
STRINGi | 9606.ENSP00000262375 |
Structurei
Secondary structure
3D structure databases
SMRi | Q96EY1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96EY1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 93 – 158 | JAdd BLAST | 66 | |
Repeati | 236 – 243 | CXXCXGXG motif | 8 | |
Repeati | 253 – 260 | CXXCXGXG motif | 8 | |
Repeati | 275 – 282 | CXXCXGXG motif | 8 | |
Repeati | 289 – 296 | CXXCXGXG motif | 8 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 223 – 301 | CR-typeAdd BLAST | 79 |
Keywords - Domaini
Repeat, Transit peptide, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0715 Eukaryota COG0484 LUCA |
GeneTreei | ENSGT00940000155280 |
HOGENOMi | HOG000203530 |
InParanoidi | Q96EY1 |
KOi | K09504 |
OMAi | MNMDDIF |
OrthoDBi | 894595at2759 |
PhylomeDBi | Q96EY1 |
TreeFami | TF105152 |
Family and domain databases
CDDi | cd06257 DnaJ, 1 hit cd10719 DnaJ_zf, 1 hit |
Gene3Di | 1.10.287.110, 1 hit |
HAMAPi | MF_01152 DnaJ, 1 hit |
InterProi | View protein in InterPro IPR012724 DnaJ IPR002939 DnaJ_C IPR001623 DnaJ_domain IPR018253 DnaJ_domain_CS IPR008971 HSP40/DnaJ_pept-bd IPR001305 HSP_DnaJ_Cys-rich_dom IPR036410 HSP_DnaJ_Cys-rich_dom_sf IPR036869 J_dom_sf |
Pfami | View protein in Pfam PF00226 DnaJ, 1 hit PF01556 DnaJ_C, 1 hit PF00684 DnaJ_CXXCXGXG, 1 hit |
PRINTSi | PR00625 JDOMAIN |
SMARTi | View protein in SMART SM00271 DnaJ, 1 hit |
SUPFAMi | SSF46565 SSF46565, 1 hit SSF49493 SSF49493, 1 hit SSF57938 SSF57938, 1 hit |
PROSITEi | View protein in PROSITE PS00636 DNAJ_1, 1 hit PS50076 DNAJ_2, 1 hit PS51188 ZF_CR, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAARCSTRWL LVVVGTPRLP AISGRGARPP REGVVGAWLS RKLSVPAFAS
60 70 80 90 100
SLTSCGPRAL LTLRPGVSLT GTKHNPFICT ASFHTSAPLA KEDYYQILGV
110 120 130 140 150
PRNASQKEIK KAYYQLAKKY HPDTNKDDPK AKEKFSQLAE AYEVLSDEVK
160 170 180 190 200
RKQYDAYGSA GFDPGASGSQ HSYWKGGPTV DPEELFRKIF GEFSSSSFGD
210 220 230 240 250
FQTVFDQPQE YFMELTFNQA AKGVNKEFTV NIMDTCERCN GKGNEPGTKV
260 270 280 290 300
QHCHYCGGSG METINTGPFV MRSTCRRCGG RGSIIISPCV VCRGAGQAKQ
310 320 330 340 350
KKRVMIPVPA GVEDGQTVRM PVGKREIFIT FRVQKSPVFR RDGADIHSDL
360 370 380 390 400
FISIAQALLG GTARAQGLYE TINVTIPPGT QTDQKIRMGG KGIPRINSYG
410 420 430 440 450
YGDHYIHIKI RVPKRLTSRQ QSLILSYAED ETDVEGTVNG VTLTSSGGST
460 470 480
MDSSAGSKAR REAGEDEEGF LSKLKKMFTS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketI3L1T6 | I3L1T6_HUMAN | DnaJ homolog subfamily A member 3, ... | DNAJA3 | 185 | Annotation score: | ||
I3L1I6 | I3L1I6_HUMAN | DnaJ homolog subfamily A member 3, ... | DNAJA3 | 122 | Annotation score: | ||
I3L1T9 | I3L1T9_HUMAN | DnaJ homolog subfamily A member 3, ... | DNAJA3 | 98 | Annotation score: | ||
I3L3D3 | I3L3D3_HUMAN | DnaJ homolog subfamily A member 3, ... | DNAJA3 | 77 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 320 | M → W in AAF66245 (PubMed:12097419).Curated | 1 | ||
Isoform 3 (identifier: Q96EY1-3) | |||||
Sequence conflicti | 33 | Y → H in BAG58345 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027965 | 75 | N → Y4 PublicationsCorresponds to variant dbSNP:rs1139653Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055728 | 3 – 186 | ARCST…PEELF → EPQAERPRLCVFPDLLRPPS AADIETWCQPY in isoform 3. 1 PublicationAdd BLAST | 184 | |
Alternative sequenceiVSP_007425 | 448 – 453 | GSTMDS → KRSTGN in isoform 2 and isoform 3. 4 Publications | 6 | |
Alternative sequenceiVSP_007426 | 454 – 480 | Missing in isoform 2 and isoform 3. 4 PublicationsAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061749 mRNA Translation: AAC29066.1 AF411044 mRNA Translation: AAL35323.1 AK295391 mRNA Translation: BAG58345.1 AK314218 mRNA Translation: BAG36892.1 AC012676 Genomic DNA No translation available. BC007225 mRNA Translation: AAH07225.1 BC011855 mRNA Translation: AAH11855.1 BC012343 mRNA Translation: AAH12343.1 Different initiation. BC014062 mRNA Translation: AAH14062.1 BC020248 mRNA Translation: AAH20248.1 BC030145 mRNA Translation: AAH30145.1 BC032100 mRNA Translation: AAH32100.1 AF244136 mRNA Translation: AAF66245.1 Sequence problems. |
CCDSi | CCDS10515.1 [Q96EY1-1] CCDS45400.1 [Q96EY1-2] CCDS66930.1 [Q96EY1-3] |
RefSeqi | NP_001128582.1, NM_001135110.2 [Q96EY1-2] NP_001273445.1, NM_001286516.1 [Q96EY1-3] NP_005138.3, NM_005147.5 [Q96EY1-1] |
Genome annotation databases
Ensembli | ENST00000262375; ENSP00000262375; ENSG00000103423 [Q96EY1-1] ENST00000355296; ENSP00000347445; ENSG00000103423 [Q96EY1-2] ENST00000431375; ENSP00000393970; ENSG00000103423 [Q96EY1-3] ENST00000612103; ENSP00000477570; ENSG00000276726 [Q96EY1-1] ENST00000614397; ENSP00000479815; ENSG00000276726 [Q96EY1-2] |
GeneIDi | 9093 |
KEGGi | hsa:9093 |
UCSCi | uc002cwk.4 human [Q96EY1-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061749 mRNA Translation: AAC29066.1 AF411044 mRNA Translation: AAL35323.1 AK295391 mRNA Translation: BAG58345.1 AK314218 mRNA Translation: BAG36892.1 AC012676 Genomic DNA No translation available. BC007225 mRNA Translation: AAH07225.1 BC011855 mRNA Translation: AAH11855.1 BC012343 mRNA Translation: AAH12343.1 Different initiation. BC014062 mRNA Translation: AAH14062.1 BC020248 mRNA Translation: AAH20248.1 BC030145 mRNA Translation: AAH30145.1 BC032100 mRNA Translation: AAH32100.1 AF244136 mRNA Translation: AAF66245.1 Sequence problems. |
CCDSi | CCDS10515.1 [Q96EY1-1] CCDS45400.1 [Q96EY1-2] CCDS66930.1 [Q96EY1-3] |
RefSeqi | NP_001128582.1, NM_001135110.2 [Q96EY1-2] NP_001273445.1, NM_001286516.1 [Q96EY1-3] NP_005138.3, NM_005147.5 [Q96EY1-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CTT | NMR | - | A | 213-303 | [»] | |
2DN9 | NMR | - | A | 93-158 | [»] | |
SMRi | Q96EY1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGridi | 114547, 121 interactors |
DIPi | DIP-33870N |
IntActi | Q96EY1, 78 interactors |
MINTi | Q96EY1 |
STRINGi | 9606.ENSP00000262375 |
PTM databases
iPTMneti | Q96EY1 |
PhosphoSitePlusi | Q96EY1 |
Polymorphism and mutation databases
BioMutai | DNAJA3 |
DMDMi | 311033374 |
Proteomic databases
EPDi | Q96EY1 |
jPOSTi | Q96EY1 |
MassIVEi | Q96EY1 |
MaxQBi | Q96EY1 |
PaxDbi | Q96EY1 |
PeptideAtlasi | Q96EY1 |
PRIDEi | Q96EY1 |
ProteomicsDBi | 17904 76466 [Q96EY1-1] 76467 [Q96EY1-2] |
Protocols and materials databases
DNASUi | 9093 |
Genome annotation databases
Ensembli | ENST00000262375; ENSP00000262375; ENSG00000103423 [Q96EY1-1] ENST00000355296; ENSP00000347445; ENSG00000103423 [Q96EY1-2] ENST00000431375; ENSP00000393970; ENSG00000103423 [Q96EY1-3] ENST00000612103; ENSP00000477570; ENSG00000276726 [Q96EY1-1] ENST00000614397; ENSP00000479815; ENSG00000276726 [Q96EY1-2] |
GeneIDi | 9093 |
KEGGi | hsa:9093 |
UCSCi | uc002cwk.4 human [Q96EY1-1] |
Organism-specific databases
CTDi | 9093 |
DisGeNETi | 9093 |
GeneCardsi | DNAJA3 |
HGNCi | HGNC:11808 DNAJA3 |
HPAi | CAB016095 HPA040875 HPA044229 |
MIMi | 608382 gene |
neXtProti | NX_Q96EY1 |
OpenTargetsi | ENSG00000103423 |
PharmGKBi | PA27410 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0715 Eukaryota COG0484 LUCA |
GeneTreei | ENSGT00940000155280 |
HOGENOMi | HOG000203530 |
InParanoidi | Q96EY1 |
KOi | K09504 |
OMAi | MNMDDIF |
OrthoDBi | 894595at2759 |
PhylomeDBi | Q96EY1 |
TreeFami | TF105152 |
Miscellaneous databases
ChiTaRSi | DNAJA3 human |
EvolutionaryTracei | Q96EY1 |
GeneWikii | DNAJA3 |
GenomeRNAii | 9093 |
Pharosi | Q96EY1 |
PROi | PR:Q96EY1 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000103423 Expressed in 224 organ(s), highest expression level in adrenal gland |
ExpressionAtlasi | Q96EY1 baseline and differential |
Genevisiblei | Q96EY1 HS |
Family and domain databases
CDDi | cd06257 DnaJ, 1 hit cd10719 DnaJ_zf, 1 hit |
Gene3Di | 1.10.287.110, 1 hit |
HAMAPi | MF_01152 DnaJ, 1 hit |
InterProi | View protein in InterPro IPR012724 DnaJ IPR002939 DnaJ_C IPR001623 DnaJ_domain IPR018253 DnaJ_domain_CS IPR008971 HSP40/DnaJ_pept-bd IPR001305 HSP_DnaJ_Cys-rich_dom IPR036410 HSP_DnaJ_Cys-rich_dom_sf IPR036869 J_dom_sf |
Pfami | View protein in Pfam PF00226 DnaJ, 1 hit PF01556 DnaJ_C, 1 hit PF00684 DnaJ_CXXCXGXG, 1 hit |
PRINTSi | PR00625 JDOMAIN |
SMARTi | View protein in SMART SM00271 DnaJ, 1 hit |
SUPFAMi | SSF46565 SSF46565, 1 hit SSF49493 SSF49493, 1 hit SSF57938 SSF57938, 1 hit |
PROSITEi | View protein in PROSITE PS00636 DNAJ_1, 1 hit PS50076 DNAJ_2, 1 hit PS51188 ZF_CR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DNJA3_HUMAN | |
Accessioni | Q96EY1Primary (citable) accession number: Q96EY1 Secondary accession number(s): B2RAJ5 Q9NYH8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2003 |
Last sequence update: | November 2, 2010 | |
Last modified: | November 13, 2019 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references