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Protein

Glucosamine 6-phosphate N-acetyltransferase

Gene

GNPNAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + D-glucosamine 6-phosphate = CoA + N-acetyl-D-glucosamine 6-phosphate.2 Publications

Kineticsi

  1. KM=26 µM for acetyl-coenzyme A1 Publication
  2. KM=97 µM for glucosamine-6-phosphate1 Publication

    Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I).
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Glucosamine 6-phosphate N-acetyltransferase (GNPNAT1)
    2. Phosphoacetylglucosamine mutase (PGM3), Phosphoacetylglucosamine mutase (PGM3), Phosphoacetylglucosamine mutase
    This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei61SubstrateCombined sources1 Publication1 Publication1
    Binding sitei175SubstrateCombined sources1 Publication1 Publication1
    Binding sitei181SubstrateCombined sources1 Publication1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAcyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.3.1.4 2681
    ReactomeiR-HSA-446210 Synthesis of UDP-N-acetyl-glucosamine
    SABIO-RKiQ96EK6
    UniPathwayi
    UPA00113;UER00529

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosamine 6-phosphate N-acetyltransferase (EC:2.3.1.42 Publications)
    Alternative name(s):
    Phosphoglucosamine acetylase
    Phosphoglucosamine transacetylase
    Gene namesi
    Name:GNPNAT1
    Synonyms:GNA1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 14

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000100522.8
    HGNCiHGNC:19980 GNPNAT1
    MIMi616510 gene
    neXtProtiNX_Q96EK6

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Endosome, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi156E → A: Reduces affinity for glucosamine-6-phosphate 6-fold. 1 Publication1
    Mutagenesisi156E → D: Slightly reduced catalytic activity. 1 Publication1

    Organism-specific databases

    OpenTargetsiENSG00000100522
    PharmGKBiPA134901326

    Polymorphism and mutation databases

    BioMutaiGNPNAT1
    DMDMi47116568

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000745531 – 184Glucosamine 6-phosphate N-acetyltransferaseAdd BLAST184

    Proteomic databases

    EPDiQ96EK6
    MaxQBiQ96EK6
    PaxDbiQ96EK6
    PeptideAtlasiQ96EK6
    PRIDEiQ96EK6
    ProteomicsDBi76418

    PTM databases

    iPTMnetiQ96EK6
    PhosphoSitePlusiQ96EK6

    Expressioni

    Gene expression databases

    BgeeiENSG00000100522 Expressed in 194 organ(s), highest expression level in testis
    CleanExiHS_GNPNAT1
    ExpressionAtlasiQ96EK6 baseline and differential
    GenevisibleiQ96EK6 HS

    Organism-specific databases

    HPAiHPA044647

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-3913338,EBI-3913338

    GO - Molecular functioni

    Protein-protein interaction databases

    BioGridi122317, 18 interactors
    IntActiQ96EK6, 5 interactors
    MINTiQ96EK6
    STRINGi9606.ENSP00000216410

    Structurei

    Secondary structure

    1184
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliQ96EK6
    SMRiQ96EK6
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ96EK6

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini39 – 184N-acetyltransferasePROSITE-ProRule annotationAdd BLAST146

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni108 – 111Substrate bindingCombined sources1 Publication1 Publication4
    Regioni120 – 122Substrate bindingCombined sources1 Publication1 Publication3
    Regioni130 – 135Acetyl-CoA bindingCombined sources1 Publication6
    Regioni151 – 152Substrate bindingCombined sources1 Publication1 Publication2
    Regioni165 – 167Acetyl-CoA bindingCombined sources1 Publication3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG3396 Eukaryota
    COG0454 LUCA
    GeneTreeiENSGT00390000008666
    HOGENOMiHOG000106325
    HOVERGENiHBG048986
    InParanoidiQ96EK6
    KOiK00621
    OMAiVGCYKSI
    OrthoDBiEOG091G0EMF
    PhylomeDBiQ96EK6
    TreeFamiTF313790

    Family and domain databases

    InterProiView protein in InterPro
    IPR016181 Acyl_CoA_acyltransferase
    IPR000182 GNAT_dom
    IPR039143 GNPNAT1
    PANTHERiPTHR13355 PTHR13355, 1 hit
    PfamiView protein in Pfam
    PF00583 Acetyltransf_1, 1 hit
    SUPFAMiSSF55729 SSF55729, 1 hit
    PROSITEiView protein in PROSITE
    PS51186 GNAT, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

    Q96EK6-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKPDETPMFD PSLLKEVDWS QNTATFSPAI SPTHPGEGLV LRPLCTADLN
    60 70 80 90 100
    RGFFKVLGQL TETGVVSPEQ FMKSFEHMKK SGDYYVTVVE DVTLGQIVAT
    110 120 130 140 150
    ATLIIEHKFI HSCAKRGRVE DVVVSDECRG KQLGKLLLST LTLLSKKLNC
    160 170 180
    YKITLECLPQ NVGFYKKFGY TVSEENYMCR RFLK
    Length:184
    Mass (Da):20,749
    Last modified:December 1, 2001 - v1
    Checksum:iE9F31803CF633BBF
    GO

    Computationally mapped potential isoform sequencesi

    There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    G3V5E4G3V5E4_HUMAN
    Glucosamine 6-phosphate N-acetyltra...
    GNPNAT1
    113Annotation score:
    G3V4W4G3V4W4_HUMAN
    Glucosamine 6-phosphate N-acetyltra...
    GNPNAT1
    163Annotation score:

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AK090577 mRNA Translation: BAC03482.1
    BC012179 mRNA Translation: AAH12179.1
    CCDSiCCDS9712.1
    RefSeqiNP_932332.1, NM_198066.3
    XP_005268069.1, XM_005268012.2
    XP_006720301.1, XM_006720238.3
    UniGeneiHs.478025

    Genome annotation databases

    EnsembliENST00000216410; ENSP00000216410; ENSG00000100522
    GeneIDi64841
    KEGGihsa:64841
    UCSCiuc001xab.4 human

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AK090577 mRNA Translation: BAC03482.1
    BC012179 mRNA Translation: AAH12179.1
    CCDSiCCDS9712.1
    RefSeqiNP_932332.1, NM_198066.3
    XP_005268069.1, XM_005268012.2
    XP_006720301.1, XM_006720238.3
    UniGeneiHs.478025

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2HUZX-ray2.67A/B1-184[»]
    2O28X-ray1.80A/B1-184[»]
    3CXPX-ray2.01A1-184[»]
    3CXQX-ray2.30A1-184[»]
    3CXSX-ray2.70A1-184[»]
    ProteinModelPortaliQ96EK6
    SMRiQ96EK6
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi122317, 18 interactors
    IntActiQ96EK6, 5 interactors
    MINTiQ96EK6
    STRINGi9606.ENSP00000216410

    PTM databases

    iPTMnetiQ96EK6
    PhosphoSitePlusiQ96EK6

    Polymorphism and mutation databases

    BioMutaiGNPNAT1
    DMDMi47116568

    Proteomic databases

    EPDiQ96EK6
    MaxQBiQ96EK6
    PaxDbiQ96EK6
    PeptideAtlasiQ96EK6
    PRIDEiQ96EK6
    ProteomicsDBi76418

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000216410; ENSP00000216410; ENSG00000100522
    GeneIDi64841
    KEGGihsa:64841
    UCSCiuc001xab.4 human

    Organism-specific databases

    CTDi64841
    EuPathDBiHostDB:ENSG00000100522.8
    GeneCardsiGNPNAT1
    HGNCiHGNC:19980 GNPNAT1
    HPAiHPA044647
    MIMi616510 gene
    neXtProtiNX_Q96EK6
    OpenTargetsiENSG00000100522
    PharmGKBiPA134901326
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG3396 Eukaryota
    COG0454 LUCA
    GeneTreeiENSGT00390000008666
    HOGENOMiHOG000106325
    HOVERGENiHBG048986
    InParanoidiQ96EK6
    KOiK00621
    OMAiVGCYKSI
    OrthoDBiEOG091G0EMF
    PhylomeDBiQ96EK6
    TreeFamiTF313790

    Enzyme and pathway databases

    UniPathwayi
    UPA00113;UER00529

    BRENDAi2.3.1.4 2681
    ReactomeiR-HSA-446210 Synthesis of UDP-N-acetyl-glucosamine
    SABIO-RKiQ96EK6

    Miscellaneous databases

    ChiTaRSiGNPNAT1 human
    EvolutionaryTraceiQ96EK6
    GenomeRNAii64841
    PROiPR:Q96EK6
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000100522 Expressed in 194 organ(s), highest expression level in testis
    CleanExiHS_GNPNAT1
    ExpressionAtlasiQ96EK6 baseline and differential
    GenevisibleiQ96EK6 HS

    Family and domain databases

    InterProiView protein in InterPro
    IPR016181 Acyl_CoA_acyltransferase
    IPR000182 GNAT_dom
    IPR039143 GNPNAT1
    PANTHERiPTHR13355 PTHR13355, 1 hit
    PfamiView protein in Pfam
    PF00583 Acetyltransf_1, 1 hit
    SUPFAMiSSF55729 SSF55729, 1 hit
    PROSITEiView protein in PROSITE
    PS51186 GNAT, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGNA1_HUMAN
    AccessioniPrimary (citable) accession number: Q96EK6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: December 1, 2001
    Last modified: September 12, 2018
    This is version 146 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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    Main funding by: National Institutes of Health

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